| ANA、抗dsDNA、抗ENA抗体联合检测对系统性红斑狼疮患者的诊断及临床意义 |
| |
| 引用本文： | 张晓梅,罗兴燕,连金泉. ANA、抗dsDNA、抗ENA抗体联合检测对系统性红斑狼疮患者的诊断及临床意义[J]. 矿产勘查, 2010, 0(1): 60-62 |
| |
| 作者姓名： | 张晓梅 罗兴燕 连金泉 |
| |
| 作者单位： | 上饶市人民医院检验科,江西上饶334000 |
| |
| 摘 要： | 目的探讨抗核抗体（ANA）、抗双链DNA抗体（抗dsDNA）、抗可提取核抗原（抗ENA）抗体联合检测对系统性红斑狼疮（SLE）患者的诊断及意义。方法分别检测92例SLE患者（SLE组）和80例健康体检者（对照组）的ANA、抗dsDNA、抗ENA抗体。ANA采用间接免疫荧光法测定，抗dsDNA采用胶体金斑点渗滤法测定，抗ENA抗体采用免疫印迹法测定。结果①SLE组患者的ANA、抗dsDNA、抗ENA抗体的阳性率分别为89．1％、47．8％、50．0％，三者联合检测的阳性率为94．5％，均明显高于对照组（P〈0．01）。②SLE组患者抗ENA各项抗体阳性率为：抗Sm抗体22．8％、抗SSA26．1％、抗SSB10．9％、抗UIRNP15．2％、抗rRNP9．7％、抗scl-70 1.1％、抗Jo-1。③SLE组患者中的40例伴发狼疮性肾炎（LN组），52例为非狼疮性。肾炎（非LN组），其中LN组抗dsDNA有30例阳性，阳性率为75．0％，非LN组抗dsDNA有14例阳性，阳性率为26．9％，两者有显著差异（P〈0．01）。结论ANA、抗dsDNA、抗ENA抗体的检测对SLE的诊断与鉴别诊断、病情的发展、疗效的观察均有重要的意义，而且联合检测可提高检出率，可以互相弥补。
|
| 关 键 词： | 抗核抗体 抗双链DNA抗体 抗可提取核抗原抗体 系统性红斑狼疮 |
The Diagnosis and the Clinical Significance of Combined Detection of ANA,Anti-dsDNA,and anti-ENA Antibody in Systematic Lupus Erthematosus |
| |
| ZHANG Xiao-mei,LUO Xing-yan,LIAN Jin-quan. The Diagnosis and the Clinical Significance of Combined Detection of ANA,Anti-dsDNA,and anti-ENA Antibody in Systematic Lupus Erthematosus[J]. Mineral Exploration, 2010, 0(1): 60-62 |
| |
| Authors: | ZHANG Xiao-mei LUO Xing-yan LIAN Jin-quan |
| |
| Affiliation: | (Department of Clinical Laboratory, Shangrao People＇s Hospital, Shangrao 334000, China) |
| |
| Abstract: | Objective To investigate the diagnostic significance of combined determination of the anti-nuclear antibody （ANA）, anti-dsDNA, anti-extractable nuclear antigens （ENA） antibody in systematic lupus erythematosus. Methods Ninety-two SLE patients （SLE group） and 80 healthy people （control group）were examined and compared. ANA was tested by indirect immunofluorescence;anti-dsDNA was tested by immofiltration assay;anti-ENA antibody was tested by immunoblotting technique. Results （1） The positive rate of ANA,anti-dsDNA and anti-ENA antibody in the SLE group patients were 89.1% ,47.8% and 50.0% respectively; the positive rate of combined determination of ANA, anti-dsDNA and anti-ENA antibody was 94.5 %. All the data in SLE group were higher than those in control group, which showed significant differences（P〈 0.01）. （2）The respective antibody positive rates of anti-ENA antibody in SLE group patients：the anti-Sin was 22.8%,the anti-SSA was 26. 1%,the anti-SSB was 10. 9%,the anti-U1RNP was 15.2%,the anti-rRNP was 9. 7%,the anti-scl-70 was 1. 1% and anti-Jo-1 was zero. （3）Of SLE group patients,40 were lupus nephtritis （LN group）,52 were not lupus nephtritis（NLN group）; the anti-dsDNA of the 30 patients in LN group was positive,whose rate was 75.0%; the 14 patients in NLN group are positive,whose rate is 26.9% ,which shows significant differences（P〈0.01）. Conclusion The determination of ANA,anti-dsDNA,and anti-ENA antibody may have important significance in diagnosis,differentiation, monitoring and treatment. The combined determination of ANA, anti-dsDNA and anti-ENA antibody could improve the determination percentage in SLE, which is complementary with each other. |
| |
| Keywords: | ANA anti-dsDNA anti-ENA antibody systematic lupus erythematosus |
| 本文献已被 维普 等数据库收录！ |
|
