| 溶血磷脂酸对人肺成纤维细胞I型胶原合成的影响 |
| |
| 引用本文： | 夏小春,李文林,石小玉,何晓璐,陈雄林,赵林. 溶血磷脂酸对人肺成纤维细胞I型胶原合成的影响[J]. 矿产勘查, 2010, 0(1): 1-4 |
| |
| 作者姓名： | 夏小春 李文林 石小玉 何晓璐 陈雄林 赵林 |
| |
| 作者单位： | [1]南昌大学研究生院医学部2007级 [2]南昌大学医学院组织学与胚胎学教研室 [3]江西省医学科学研究所,南昌330006 [4]九江学院组织学与胚胎学教研室,江西九江332000 |
| |
| 基金项目： | 基金项目：国家自然科学基金（30860118）;江西省卫生厅科技课题（20062016,20072017）;江西省教育厅科技课题[赣教技字（2007）81号] |
| |
| 摘 要： | 目的探讨溶血磷脂酸（lysophosphatidic acid，LPA）对人肺成纤维细胞（HFL-1）I型胶原（procollagentype I，PCOL I）合成的影响。方法将HFL-1培养后置于6孔板中，随机分为6组：对照组，LPAI、Ⅱ、Ⅲ组（用1umol．L-1。的LPA刺激6、12、24h后收集细胞），IL-13组（用100ug·L-1的IL-13作用48h后收集细胞），LPA＋IL-13组（用1umol·L-1的LPA刺激12h、100ug·L-1的IL-13刺激48h后收集细胞），每组3孔。用RT—PCR方法检测PCOLImRNA的表达；用免疫组化方法检测PCOLI蛋白的表达。结果免疫组化与RT—PCR方法均显示LPA刺激后，PCOLI的表达降低，IL-13刺激后，PCOLI的表达增高，且LPA＋IL-13组PCOLI表达比LPA组较高，比IL-13组较低。结论LPA可以下调HFL-1PCOLI的合成。
|
| 关 键 词： | 溶血磷脂酸 人肺成纤维细胞 白介素13 I型胶原 |
The Effect of Lysophosphatidic Acid on Procollagen Type I Gene Expression in Human Lung Fibroblasts |
| |
| XIA Xiao-chun,LI Wen-lin,SHI Xiao-yu,HE Xiao-lu,CHEN Xiong-lin,ZHAO Lin. The Effect of Lysophosphatidic Acid on Procollagen Type I Gene Expression in Human Lung Fibroblasts[J]. Mineral Exploration, 2010, 0(1): 1-4 |
| |
| Authors: | XIA Xiao-chun LI Wen-lin SHI Xiao-yu HE Xiao-lu CHEN Xiong-lin ZHAO Lin |
| |
| Affiliation: | 1a. 2007 Grade of Medical Department of Graduate School; 1b. Department of Histology and Embryology ,Medical College, Nanchang University ; 2. Institute of Medical Sciences, Nanchang 330006 ,China ; 3. Department of Histology and Embryology , Jiujiang College , Jiujiang 332000,China) |
| |
| Abstract: | Objective To investigate the effect of lysophosphatidic acid （LPA） on procollagen type I （PCOL I ） gene expression in human lung fibroblasts（HFL-1）. Methods HFL-1 cultured and then seeded 6-well plates,and then divided into 6 groups：control group, LPA I . II .III group（cells were stimulated with 1 umol . L- 1 LPA 6,12,24 h respectively,and then collected）, IL-13 group （cells were stimulated with 100 ug . L-1 IL-13 48 h and then collected） ,LPA＋IL-13 group（cells were stimulated with 1 umol . L-1 LPA 12 h and 100 ug . L-1 IL-13 48 h,then collected）,cells were cultured in 3 wells each group. The expression of PCOL I mRNA was evaluated by RT-PCR. The expression of PCOL I protein was evaluated by immunohistochemisty. Results The results of RT-PCR and immunohistochemisty both demonstrated that LPA could reduce the expression of PCOL I in HFL-1. In addition, the expression of PCOL I in IL-13 group was higher than that in control group,and IL-13＋LPA group was higher than that in LPA group but lower than IL-13 group. Conclusion The expression of PCOL I is down-regulated when HFL-1 cells are stimulated with LPA. |
| |
| Keywords: | lysophosphatidic acid HFL-1 IL-13 procollagen type I |
| 本文献已被 维普 等数据库收录！ |
|
