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| 无溶剂体系中酶催化合成结构脂质条件初探 | |
| 引用本文： | 陈翔,王瑛瑶,栾霞,张蕊,赵玉生. 无溶剂体系中酶催化合成结构脂质条件初探[J]. 中国油脂, 2010, 35(3) |
| 作者姓名： | 陈翔 王瑛瑶 栾霞 张蕊 赵玉生 |
| 作者单位： | 1. 国家粮食局,科学研究院,北京,100037;郑州轻工业学院,食品工程系,郑州,450002 2. 国家粮食局,科学研究院,北京,100037 3. 郑州轻工业学院,食品工程系,郑州,450002 |
| 摘 要： | 以菜籽油和辛酸为原料,在无溶剂体系中用脂肪酶催化酸解合成结构脂质。对6种不同来源的脂肪酶进行筛选,结果表明Lipozyme RMIM催化活性高、Sn-1,3位特异性强。以Lipozyme RMIM为催化用酶,考察了反应时间、反应温度、底物比(菜籽油与辛酸摩尔比)、加酶量、体系水分含量对酸解反应的影响。结果表明,在反应时间15 h,反应温度50℃,底物比1∶4,加酶量10%条件下,辛酸合成率达40%。 |
| 关 键 词： | 菜籽油 辛酸 脂肪酶 酸解 结构脂质 |
Enzymatic synthesis of structured lipids in a solvent-free system |
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| CHEN Xiang,WANG Yingyao,LUAN Xia,ZHANG Rui,ZHAO Yusheng. Enzymatic synthesis of structured lipids in a solvent-free system[J]. China Oils and Fats, 2010, 35(3) | |
| Authors: | CHEN Xiang WANG Yingyao LUAN Xia ZHANG Rui ZHAO Yusheng |
| Abstract: | Lipase-catalyzed acidolysis of rapeseed oil with caprylic acid was performed to produce low-calorie structured lipids in a solvent-free system.Six commercial lipases were screened for their ability to catalyze acidolysis and the results indicated that the immobilized Lipozyme RM IM had more catalysis ability and Sn-1,3-specificity.Acidolysis parameters,such as time,reaction temperature,substrate molar ratio,enzyme load,water content were investigated.Under these conditions of 10% of the total weight of substrates for immobilized lipase,molar ratio of rapeseed oil to caprylic acid 1∶ 4 and reaction 15 h at 50 ℃,the incorporation of caprylic acid into rapeseed oil reached 40%. |
| Keywords: | rapeseed oil caprylic acid lipase acidolysis structured lipids |
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