| 高效液相色谱柱前衍生法测定蜜饯中的甜蜜素 |
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| 引用本文： | 何良兴,林赛君,柯春晖,郭柏坤. 高效液相色谱柱前衍生法测定蜜饯中的甜蜜素[J]. 食品科学, 2009, 30(4): 204-206. DOI: 10.7506/spkx1002-6630-200904043 |
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| 作者姓名： | 何良兴 林赛君 柯春晖 郭柏坤 |
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| 作者单位： | 1.杭州市余杭区质量计量监测中心 2.杭州市质量技术监督检测院 |
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| 摘 要： | 目的：建立简便、准确、灵敏的测定蜜饯中甜蜜素含量方法。方法：在酸性介质条件下，溶液中的甜蜜素同次氯酸钠溶液发生反应，其衍生产物在紫外-HPLC 法下测定。结果：方法最低检出限为0.05g/kg，其在0.02～1.00mg/ml 范围内呈线性关系，相关系数为0.9995，方法的相对标准偏差为1.56%～4.49%，回收率为91.5%～98.5%，检测结果与GB/T5009.97 - 2003 气相色谱法无显著差异。结论：本法灵敏度高，操作简单，稳定性和重复性好，适合于蜜饯中甜蜜素含量的测定。
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| 关 键 词： | 甜蜜素 高效液相色谱 柱前衍生 |
| 收稿时间： | 2008-02-27 |
Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization |
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| HE Liang-xing,LIN Sai-jun,KE Chun-hui,GUO Bai-kun. Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization[J]. Food Science, 2009, 30(4): 204-206. DOI: 10.7506/spkx1002-6630-200904043 |
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| Authors: | HE Liang-xing LIN Sai-jun KE Chun-hui GUO Bai-kun |
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| Affiliation: | 1. Supervising and Testing Center for Quality and Measurement of Yuhang District of Hangzhou, Hangzhou 311199, China ；2. Hangzhou Institute of Test and Calibration for Quality and Technology Supervision, Hangzhou 301110, China |
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| Abstract: | Objective: to establish a simple, accurate and sensitive method for determination of cyclamate in preserved fruits by high-performance liquid chromatography with pre-column derivatization. Method: Through the reaction between cyclamate and sodium hypochlorite under acid medium, N,N-dichloro- cyclohexylamine was determined by UV-HPLC. Results: The detection limit is 0.05 g/kg. The calibration curve is linear in the range of 0.02 to 1.00 mg/ml with a correlation coefficient of 0.9995. The relative standard deviation is 1.56% to 4.49% and the recovery rate is in the range of 91.5% to 98.5%. The determination result has no significant deference from that by the method of GB5009.97 - 2003. Conclusion: The method is sensitivity, simple and stable and has good repetition. It can be used for the determination of cyclamate in preserved fruit. |
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| Keywords: | cyclamate high-performance liquid chromatography pre-column derivatization |
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