| 超高效液相色谱-串联质谱法测定食用油中的叔丁基对苯二酚 |
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| 引用本文： | 张朋杰,张宪臣,张 静,王 勇. 超高效液相色谱-串联质谱法测定食用油中的叔丁基对苯二酚[J]. 食品科学, 2009, 30(6): 143-145. DOI: 10.7506/spkx1002-6630-200906030 |
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| 作者姓名： | 张朋杰 张宪臣 张 静 王 勇 |
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| 作者单位： | 中山出入境检验检疫局 |
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| 基金项目： | 广东省自然科学基金资助项目(7301741)；中山市科技计划资助项目(20073A155) |
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| 摘 要： | 目的：建立一套稳定可靠的使用超高效液相色谱- 串联质谱(UPLC-MS/MS)测定食用油中的抗氧化剂叔丁基对苯二酚(TBHQ)的方法。方法：用乙腈振荡萃取样品中的TBHQ，以乙腈- 水为流动相洗脱，以反相C18 柱作为分离柱，以电喷雾离子源为接口，负离子模式扫描，选择监测(SRM)模式检测，外标法定量。结果：本方法的检出限为0.10mg/kg，在0.05～2.5μg/ml 浓度范围内呈良好线性，相关系数大于0.999，回收率83%～115%。结论：实验结果表明该方法灵敏度高，检出限低，确证能力强，分析时间短，能够准确的测定食用油中的抗氧化剂TBHQ 的含量。
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| 关 键 词： | 超高效液相色谱-串联质谱法 选择监测(SRM)模式 叔丁基对苯二酚 食用油 |
| 收稿时间： | 2008-05-21 |
Determination of Tertiary Butyhydroquinone in Edible Oils by Ultra Performance Liquid Chromatography-Tantem ass Spectrometry |
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| ZHANG Peng-jie,ZHANG Xian-chen,ZHANG Jing,WANG Yong. Determination of Tertiary Butyhydroquinone in Edible Oils by Ultra Performance Liquid Chromatography-Tantem ass Spectrometry[J]. Food Science, 2009, 30(6): 143-145. DOI: 10.7506/spkx1002-6630-200906030 |
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| Authors: | ZHANG Peng-jie ZHANG Xian-chen ZHANG Jing WANG Yong |
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| Affiliation: | (Zhongshan Exit-Entry Inspection and Quarantine Bureau, Zhongshan 528403, China) |
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| Abstract: | Objective: To develop a stable and reliable method of ultra performance liquid chromatography-tantem mass pectrometry (UPLC-TMS) for determining tertiary butyhydroquinone (TBHQ) content in edible oils. Methods: TBHQ in amples was extracted with acetonitrile, eluted by the mobile phase of acetonitrile-water with reserved C18 column as the separation olumn, scanned by negative ion detection mode with electrospray ionization as joint, detected by selected reaction monitoring SRM) mode and calibrated by external standard. Result: The detection limit of TBHQ is 0.10 mg/kg, the correlation coefficient f linear calibration curve is over 0.999 in the range of 0.05 to 2.5 μg/ml, and the recovery rate is 83% to 115%. Conclusion: The ethod is of high sensitivity, low detection limit, good determination capacity and short analysis time, and can be used to ccurately determine TBHQ content in edible oil. |
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| Keywords: | ultra performance liquid chromatography-tantem mass spectrometry (UPLC-TMS) elected reaction monitoring SRM) mode tertiary butyhydroquinone (TBHQ) edible oil |
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