| 鸡肉组织中氯羟吡啶残留超高效液相色谱检测方法研究 |
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| 引用本文： | 闫小峰,孙志文. 鸡肉组织中氯羟吡啶残留超高效液相色谱检测方法研究[J]. 食品科学, 2009, 30(10): 209-211. DOI: 10.7506/spkx1002-6630-200910047 |
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| 作者姓名： | 闫小峰 孙志文 |
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| 作者单位： | 北京市兽药监察所 |
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| 摘 要： | 对鸡肉组织中氯羟吡啶残留高效液相色谱方法进行了研究，建立了鸡肉组织中氯羟吡啶残留超高效液相色谱检测方法。取鸡肉样品，用乙腈提取，正己烷脱脂，过氧化铝B 柱，用20ml 甲醇洗脱，减压蒸干，残留物用甲醇溶解，紫外检测器在267nm 波长下测定，流动相为乙腈:水(10:90，V/V)。氯羟吡啶浓度在10～1000μg/L 范围内，呈良好的线性，相关系数＞ 0.999，在10、50、100μg/kg 三个添加水平，平均回收率60.5%～94.6%，日内变异系数在4.2%～10.2%之间，日间变异系数在6.6%～12.7%之间，方法检测限为5μg/kg，定量限为15μg/kg。
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| 关 键 词： | 氯羟吡啶 超高效液相色谱 鸡肉 残留 |
| 收稿时间： | 2008-07-23 |
Determination of Clopidol Residue in Chicken by Ultra Performance Liquid Chromatography |
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| YAN Xiao-feng,SUN Zhi-wen. Determination of Clopidol Residue in Chicken by Ultra Performance Liquid Chromatography[J]. Food Science, 2009, 30(10): 209-211. DOI: 10.7506/spkx1002-6630-200910047 |
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| Authors: | YAN Xiao-feng SUN Zhi-wen |
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| Affiliation: | (Beijing Institute of Veterinary Drug Control, Beijing 100107, China) |
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| Abstract: | An ultra performance liquid chromatography (UPLC) method for determination of clopidol in chicken was developed. The clopidol residue in chicken was extracted by acetonitrile, defatted by n-hexane, purified by alumina B cartridges, analyzed by HPLC with UV detector at 267 nm and acetonitrile-water (10:90, V/V) solvent as mobile phase, and quantified by using external standard calibration curve. The results showed that there was a nice linear relationship (r ＞ 0.999) when clopidol concentration was in the range of 10 to 1000 μg/L. The average recoveries at three addition levels (10, 50 and 100 μg/kg) were 60.5% to 94.6%. The within-day and inter-day variation coefficients were between 4.2% and 10.2% and between 6.6% and 12.7%, respectively. The limit of detection was 5 μg/kg, and the limit of quantification was 15 μg/kg. |
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| Keywords: | clopidol ultra performance liquid chromatography (UPLC) chicken residue |
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