| 紫果西番莲果皮中多酚提取工艺的比较及优化 |
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| 引用本文： | 张玲, 马于然, 李春海. 紫果西番莲果皮中多酚提取工艺的比较及优化[J]. 食品工业科技, 2018, 39(16): 171-176. DOI: 10.13386/j.issn1002-0306.2018.16.031 |
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| 作者姓名： | 张玲 马于然 李春海 |
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| 作者单位： | 1. 广东省岭南特色果蔬加工及应用工程技术研究中心, 广东普通高校食品科学创新团队, 广东高校果蔬加工与贮藏工程技术开发中心, 广东茂名 525000;2. 广东石油化工学院环境与生物工程学院, 广东茂名 525000 |
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| 基金项目： | 广东普通高校食品科学创新团队项目(2016KCXTD020)广东省教育厅平台项目(2012gczxB001)。广东省岭南特色果蔬加工关键技术及应用工程技术研究中心项目(粤科函产学研字[2015]1487号) |
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| 摘 要： | 以紫果西番莲果皮为原料,研究乙醇提取和纤维素酶辅助提取果皮中多酚的最佳工艺条件并对提取效果进行比较。以多酚得率为指标,通过单因素试验考察各个因素对多酚提取效果的影响;采用L9(34)正交试验优化了纤维素酶辅助提取工艺条件。结果表明,乙醇提取法的最适工艺条件为乙醇体积分数60%,液料比30:1 mL/g,提取时间150 min,浸提温度为40 ℃,多酚得率为(11.648±0.118) mg/g;酶法辅助提取的最佳工艺条件为纤维素酶用量为25 mg/g,液料比为35:1 mL/g,酶解温度40 ℃,酶解时间为60 min,pH=5,多酚得率为(15.096±0.0948) mg/g。比较两种工艺条件下多酚最大得率可知,纤维素酶法辅助提取比乙醇提取法的多酚得率高出29.6%,证明纤维素酶对西番莲果中细胞壁的破碎效果较好,可以提高果皮中多酚的提取得率。
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| 关 键 词： | 西番莲果皮 多酚 乙醇提取 纤维素酶 得率 |
| 收稿时间： | 2017-11-15 |
Comparison and Optimization of Extraction Process of Polyphenols from Passiflora edulis Peel |
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| ZHANG Ling, MA Yu-ran, LI Chun-hai. Comparison and Optimization of Extraction Process of Polyphenols from Passiflora edulis Peel[J]. Science and Technology of Food Industry, 2018, 39(16): 171-176. DOI: 10.13386/j.issn1002-0306.2018.16.031 |
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| Authors: | ZHANG Ling MA Yu-ran LI Chun-hai |
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| Affiliation: | 1. Technology Research Center for Lingnan Characteristic Fruits & Vegetables Processing and Application Engineering of Guangdong Province, Food Science Innovation Team of Guangdong Higher Education Institutes, Development Center of Technology for Fruit & Vegetable Storage and Processing Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China;2. College of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China |
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| Abstract: | With the Passiflora edulis peel as raw material,the optimum conditions of ethanol extraction and cellulose-assisted extraction of polyphenols in the peel were studied and the extraction effects were compared. Taking polyphenol yield as an index,the influence of various factors on the extraction of polyphenols was investigated by single factor test. The L9(34)orthogonal test was used to optimize the process conditions of cellulose extraction. The results showed that the optimal conditions of ethanol extraction were ethanol 60%,liquid to solid ratio 30:1 mL/g,extraction time 150 min,extraction temperature 40 ℃,and the polyphenol yield was(11.648±0.118) mg/g. And the optimal extraction conditions for enzymatic extraction were as follows:The amount of cellulase 25 mg/g,the liquid to material ratio 35:1 mL/g,the enzymolysis temperature 40 ℃,the enzymolysis time 60 min,pH=5,and the polyphenol yield was(15.096±0.948) mg/g. By comparing the maximum yield of polyphenols in two kinds of processing conditions,the extraction rate of polyphenols from cellulase assisted extraction method was 29.6% higher than that of ethanol extraction method. It proved that cellulase had better effect on the cell wall fragmentation of Passiflora edulis,and which would improve the extraction yield of polyphenols in peel. |
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| Keywords: | Passiflora edulis peel polyphenol ethanol extraction cellulose yield |
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