| 甘草次酸对小鼠急性放射性肺损伤的防治作用 |
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| 引用本文： | 张纬建,许建华,张洁旻,陈金梅,陈秀英,杨梅春,吕文龙,洪金省. 甘草次酸对小鼠急性放射性肺损伤的防治作用[J]. 金属学报, 2015, 20(6): 629-633 |
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| 作者姓名： | 张纬建 许建华 张洁旻 陈金梅 陈秀英 杨梅春 吕文龙 洪金省 |
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| 作者单位： | 福建医科大学附属第一医院 |
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| 基金项目： | 福建省自然科学基金计划资助项目;福建省医学创新课题;福建省卫生厅青年科研课题 |
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| 摘 要： | 摘要 : 目的 建立C57BL/6小鼠急性放射性肺损伤模型，观察甘草次酸对急性放射性肺损伤的干预作用。方法 成年C57BL/6雌性小鼠90只，随机分为三组，每组30只：(1)空白对照组：不照射+生理盐水10ml/（kg.d）灌胃；(2)单纯照射组：照射+生理盐水10ml/（kg.d）灌胃；(3)甘草次酸组（GA组）：照射+甘草次酸40mg /（kg.d）灌胃。IR组和GA组小鼠予6MV-X线12Gy全肺单次照射。照射后第2天、17天、30天随机从各组分别选取10只小鼠处死。取双侧肺组织行HE染色、Masson染色观察肺组织形态变化。结果 CG组小鼠肺组织结构正常。IR组小鼠肺组织在照射后第2天、17天以渗出为主，肺泡腔及间隔可见渗出、充血，伴有炎症细胞的浸润。照射后第30天，肺泡壁间隔增宽、肺泡结构变形、炎性细胞浸润，并可见胶原纤维沉积。GA组在不同时间点，肺泡炎及胶原沉积的情况均较IR组改善，任一时间点两组之间有显著性差异(P<0.05)。结论 C57BL/6小鼠全肺单次照射12Gy X射线可建立急性放射性肺损伤动物模型，甘草次酸可以减轻急性放射性肺损伤的炎症反应及改善胶原沉积。
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| 收稿时间： | 2014-10-29 |
| 修稿时间： | 2014-12-11 |
Build up acute radiation-induced lung injury model in mice and observe the protect effect of Glycyrrhetic acid |
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| Abstract: | Abstract Purpose: Establish acute radiation-induced lung injury model in mice, and observe the protect effect of glycyrrhetinic acid on the model. Materials and Methods: 90 adult C57BL /6 female mice were randomly divided into three groups: (1) control group (Conrol group): no irradiation; (2) glycyrrhetinic group (GA group): irradiation + glycyrrhetinic acid 40mg/kg by gavage; (3) irradiation group (IR group): irradiation + saline 0.01ml/g by gavage. Irradiation groups were exposed to a single fraction of X-rays (12 Gy) to the thorax. Two days ,17 days and 30 days after irradiation, random 10 mice of every group were executed. Histopathological analyses of lung tissues were done with HE and Masson staining to assess lung injury. Results: The control group showed no obvious inflammatory changes. However, massive infiltration of inflammatory cells in alveolae and alveolar septal were observed in the IR group on day-2 and day-17. Whereas, alveolar septal thickening, alveolar structure deformation, the infiltration of inflammatory cells, and collagen fibers deposition were observed in the IR group on day-30. Histologic scoring demonstrated that GA administration mitigated alveolitis and collagen deposition significantly as compared with NS administration at each time point (P<0.05). Conclusions: Lungs of C57BL /6 mice irradiated with a single fraction of 12 Gy X-ray can build up an animal model of acute radiation-induced lung injury. Glycyrrhetinic acid can alleviate the inflammatory reaction and collagen deposition of acute radiation-induced lung injury phase. |
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