| 以恶臭假单胞菌发酵制备D-氨基葡萄糖酸 |
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| 引用本文： | 袁静,孟笑,何冰芳. 以恶臭假单胞菌发酵制备D-氨基葡萄糖酸[J]. 过程工程学报, 2009, 9(1): 123-127 |
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| 作者姓名： | 袁静 孟笑 何冰芳 |
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| 作者单位： | 南京工业大学制药与生命科学学院,江苏,南京,210009;南京工业大学制药与生命科学学院,江苏,南京,210009;南京工业大学制药与生命科学学院,江苏,南京,210009 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，江苏省自然科学基金 |
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| 摘 要： | 从土样中分离到D-氨基葡萄糖酸高产菌株GNA5，综合Biolog细菌自动鉴定系统与16S rDNA序列分析结果，鉴定为恶臭假单胞菌(Pseudomonas putida). P. putida GNA5能耐受较高浓度底物D-氨基葡萄糖，可发酵积累D-氨基葡萄糖酸. 初步优化培养基为(g/L)：D-氨基葡萄糖盐酸盐30，葡萄糖5，尿素5, KH2PO4 2, MgSO4×7H2O 0.5, CaCO3 10, pH 7.0. 菌株GNA5在该培养基中发酵48 h可制备25.5 g/L的D-氨基葡萄糖酸，摩尔转化率达93.91%. 自行筛选的P. putida GNA5显示了底物转化能力强及产物分解能力弱的特点，具有进一步产业化开发的潜力.
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| 关 键 词： | D-氨基葡萄糖酸 氧化发酵 恶臭假单胞菌 D-氨基葡萄糖 |
| 收稿时间： | 2008-06-18 |
| 修稿时间： | 2008-08-21 |
Production of D-Glucosaminic Acid by Fermentation with Pseudomonas putida |
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| YUAN Jing,MENG Xiao,HE Bing-fang. Production of D-Glucosaminic Acid by Fermentation with Pseudomonas putida[J]. Chinese Journal of Process Engineering, 2009, 9(1): 123-127 |
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| Authors: | YUAN Jing MENG Xiao HE Bing-fang |
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| Affiliation: | College of Life Science and Pharmacy, Nanjing University of TechnologyCollege of Life Science and Pharmacy, Nanjing University of TechnologyCollege of Life Science and Pharmacy, Nanjing University of Technology, The Provincial Key Laboratory of Industrial Biotechnology |
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| Abstract: | Strain GNA5 producing D-glucosaminic acid from D-glucosamine was isolated from soil samples.It was identified as Pseudomonas putida based on the assay of Biology Automated Microbiology Identification System and analysis of 16S ribosomal DNA sequence.P.putida GNA5 could grow in the medium containing high concentration of D-glucosamine, and D-glucosaminic acid was stably accumulated in the oxidative fermentation process.The preliminary optimized culture medium composition(g/L) for the production of D-glucosam... |
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| Keywords: | D-glucosaminic acid oxidative fermentation Pseudomonas putida D-glucosamine |
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