| 玉竹多糖对D-半乳糖诱导A7r5细胞衰老的保护作用 |
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| 引用本文： | 李明慧,侯俊宇,胡迪,刘静,赵梦丹,葛俊宏,申野,李坦,彭钰博,刘鹏,孙新. 玉竹多糖对D-半乳糖诱导A7r5细胞衰老的保护作用[J]. 食品工业科技, 2022, 43(6): 380-388. DOI: 10.13386/j.issn1002-0306.2021070322 |
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| 作者姓名： | 李明慧 侯俊宇 胡迪 刘静 赵梦丹 葛俊宏 申野 李坦 彭钰博 刘鹏 孙新 |
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| 作者单位： | 1.北华大学基础医学院，吉林吉林 1320132.吉林医药学院药学院，吉林吉林 1320133.北华大学药学院，吉林吉林 1320134.北华大学公共卫生学院，吉林吉林 1320135.北华大学口腔医学院，吉林吉林 132013 |
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| 基金项目： | 吉林省科技厅重点实验室项目（20200201178JC）；吉林省发展改革委员会项目（2021C007）；吉林医药学院博士基金（JYBS2021026LK）；吉林医药学院横向科研项目（2021-HX002）。 |
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| 摘 要： | 目的:研究玉竹多糖(Polygonatum odoratum polysaccharides,POP)对D-半乳糖(D-galactose,D-gal)诱导大鼠胸大动脉平滑肌细胞(A7r5)衰老的保护作用.方法:采用水提醇沉法提取POP,并通过苯酚-硫酸法测得POP中总糖含量.应用D-gal建立A7r5细胞衰老模型,采...
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| 关 键 词： | 玉竹多糖 细胞衰老 β-半乳糖苷酶 活性氧 线粒体膜电位 |
| 收稿时间： | 2021-07-27 |
Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose |
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| LI Minghui,HOU Junyu,HU Di,LIU Jing,ZHAO Mengdan,GE Junhong,SHEN Ye,LI Tan,PENG Yubo,LIU Peng,SUN Xin. Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose[J]. Science and Technology of Food Industry, 2022, 43(6): 380-388. DOI: 10.13386/j.issn1002-0306.2021070322 |
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| Authors: | LI Minghui HOU Junyu HU Di LIU Jing ZHAO Mengdan GE Junhong SHEN Ye LI Tan PENG Yubo LIU Peng SUN Xin |
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| Affiliation: | 1.College of Basic Medicine, Beihua University, Jilin 132013, China2.College of Pharmacy, Jilin Medical College, Jilin 132013, China3.College of Pharmacy, Beihua University, Jilin 132013, China4.College of Public Health, Beihua University, Jilin 132013, China5.College of Stomatology, Beihua University, Jilin 132013, China |
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| Abstract: | Objective: The protective effect of Polygonatum odoratum polysaccharides (POP) on D-galactose (D-gal) induced senescence in rat aortic smooth muscle cells (A7r5) was investigated. Methods: Firstly, the POP was extracted by the water extraction and alcohol precipitation methods. Next, the phenol concentrated sulfuric acid method was applied to further measure the total sugar content of POP. In addition, the A7r5-senescent cell model was established by the D-galactose treatment. Finally, the MTT assay, β-galactosidase staining, reactive oxygen species (ROS) staining as well as JC-1 staining were applied to further detect the protective effect of POP in A7r5-senescent cells induced by D-galactose. Results: This study found an yield of POP was 8.23% and its total sugar content was 76.48%±0.036%. As a result, the MTT assay showed the viability of the A7r5 cells was increased to 174.89%±3.30% after 24 h treatment by 400 μg/mL of POP, while it was decreased to 65.93%±1.63% after 48 h treatment by 40 mg/mL of D-galactose. Interestingly, when a concentration of POP was 100 μg/mL, this study had found the most significant protective effect in A7r5-senescent cells induced by D-galactose, which increased its cell viability to 226.87%±12.58% as compared with the control group (P<0.001). Additionally, POP decreased the total β-galactosidase, inhibited its ROS levels, increased the number of positive cells after JC-1 staining, as well as protected the stability of mitochondrial membrane potential in A7r5 cells induced by D-galactose. Conclusion: POP had a potential role in the suppression of oxidative stress and cellular senescence in A7r5 cells induced by D-galactose, which could further support its anti-aging effects in vitro. |
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