| 基于UHPLC-Q-TOF/MS法同时检测猪肉中主要抗生素类兽药残留 |
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| 引用本文： | 裴斐,韩萍,王杰,马宁,苏安祥,杨文建,胡秋辉. 基于UHPLC-Q-TOF/MS法同时检测猪肉中主要抗生素类兽药残留[J]. 食品工业科技, 2022, 43(10): 298-304. DOI: 10.13386/j.issn1002-0306.2021080169 |
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| 作者姓名： | 裴斐 韩萍 王杰 马宁 苏安祥 杨文建 胡秋辉 |
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| 作者单位： | 南京财经大学食品科学与工程学院，江苏省粮油品质控制及深加工技术重点实验室，江苏省现代粮食流通与安全协同创新中心，江苏南京 210023 |
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| 基金项目： | 江苏高校“青蓝工程”资助项目；江苏省高校优势学科建设工程资助项目（PAPD）。 |
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| 摘 要： | 建立了QuEChERS净化结合超高效液相色谱串联四级杆飞行时间质谱(UHPLC-Q-TOF/MS)法同时筛查猪肉中10种抗生素类兽药残留量的分析方法.样品经过Na2EDTA-Mcllvaine缓冲溶液-0.1％甲酸乙腈-NaCl-无水Na2SO4-C18的提取净化.通过Infinity Lab Poroshell 12...
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| 关 键 词： | QuEChERS 超高效液相色谱串联四级杆飞行时间质谱(UHPLC-Q-TOF/MS) 猪肉 抗生素 |
| 收稿时间： | 2021-08-17 |
Simultaneous Determination of Major Antibiotics Veterinary Drug Residues in Pork by UHPLC-Q-TOF/MS |
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| PEI Fei,HAN Ping,WANG Jie,MA Ning,SU Anxiang,YANG Wenjian,HU Qiuhui. Simultaneous Determination of Major Antibiotics Veterinary Drug Residues in Pork by UHPLC-Q-TOF/MS[J]. Science and Technology of Food Industry, 2022, 43(10): 298-304. DOI: 10.13386/j.issn1002-0306.2021080169 |
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| Authors: | PEI Fei HAN Ping WANG Jie MA Ning SU Anxiang YANG Wenjian HU Qiuhui |
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| Affiliation: | College of Food Science and Engineering, Nanjing University of Finance and Economics, Jiangsu Key Laboratory of Grain and Oil Quality Control and Deep Processing, Jiangsu Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China |
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| Abstract: | A method for simultaneous determination of 10 veterinary antibiotics residues in pork by QuEChERS purification combined with ultra performance liquid chromatography tandem quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF/MS) was developed. The sample was extracted and purified by Na2EDTA-Mcllvaine buffer solution-0.1% formic acid acetonitrile-NaCl-anhydrous Na2SO4-C18. The separation was performed on an Infinity Lab Poroshell 120 EC-C18 column and analyzed under the positive ion multi-reaction monitoring mode. The results showed that the correlation coefficients of 10 veterinary drugs were greater than 0.99, and the linear range was 5.0~100.0 μg/kg. The average recoveries were 72.3%~117.3% at the spiked levels of 5, 10, 20 μg/kg. The intra-day relative standard deviations (RSD) was 0.9% to 10.4%, and the within-day RSD was 1.1% to 13.4%. The limit of detection (LOD) was 0.25~1.0 μg/kg, and the limit of quantification (LOQ) was 0.5~5 μg/kg. This method was used to detect 50 fresh raw pork samples purchased in the market. One sample was positive for tetracycline, and none of the other samples were detected. The method was simple and fast to operate, had good precision and limit of quantification, and was suitable for the rapid detection of 10 antibiotic veterinary drugs in pork. |
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