| HPLC法检测蜜饯中20种合成着色剂含量 |
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| 引用本文： | 冉丹,罗苏苏,张可欣,肖玥惠子,王淑霞,徐思敏,李萌. HPLC法检测蜜饯中20种合成着色剂含量[J]. 食品工业科技, 2022, 43(16): 281-289. DOI: 10.13386/j.issn1002-0306.2021100307 |
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| 作者姓名： | 冉丹 罗苏苏 张可欣 肖玥惠子 王淑霞 徐思敏 李萌 |
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| 作者单位： | 湖南省产商品质量检验研究院食品安全监测与预警湖南省重点实验室，湖南长沙 410007 |
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| 基金项目： | 湖南省科技创新平台与人才计划项目（2019TP1058）。 |
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| 摘 要： | 建立蜜饯中柠檬黄、新红、苋菜红、靛蓝、丽春红S、胭脂红、喹啉黄、日落黄、诱惑红、亮蓝、酸性红44、酸性红、食品红1、橙黄1、酸性红50、专利蓝V、赤藓红、酸性橙2、酸性橙8、亮蓝G共20种合成着色剂的高效液相色谱测定方法。试样用甲醇/氨水溶液提取，混合型弱阴离子反相固相萃取柱净化，甲醇/20 mmol/L乙酸铵为流动相梯度洗脱，二极管阵列检测器多波长检测，外标法定量。结果表明，该方法在0.1~20 μg/mL的线性范围内相关系数均大于0.999，加标回收率范围为84.0%~104.5%，相对标准偏差（RSD）为1.3%~5.3%。该方法操作便捷、稳定性好、回收率高，适用于蜜饯中20种合成着色剂的同时分析检测。该方法可以扩展应用于其他同结构性质的色素分析，对其他食品类别中色素的检测也具有参考意义。
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| 关 键 词： | 合成色素 甲醇/氨水提取 混合型弱阴离子反相固相萃取 高效液相色谱法 蜜饯 |
| 收稿时间： | 2021-11-01 |
Determination of 20 Kinds of Synthetic Colorants in Candied Fruit by HPLC |
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| RAN Dan,LUO Susu,ZHANG Kexin,XIAO Yuehuizi,WANG Shuxia,XU Simin,LI Meng. Determination of 20 Kinds of Synthetic Colorants in Candied Fruit by HPLC[J]. Science and Technology of Food Industry, 2022, 43(16): 281-289. DOI: 10.13386/j.issn1002-0306.2021100307 |
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| Authors: | RAN Dan LUO Susu ZHANG Kexin XIAO Yuehuizi WANG Shuxia XU Simin LI Meng |
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| Affiliation: | Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Hunan Institute of Product Quality Inspectionb, Changsha 410007, China |
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| Abstract: | A method for the determination of tartrazine, xinhong, amaranth, indigo, ponceau S, carmine, quinoline yellow, sunset yellow, temptation red, bright blue, acid red 44, acid red, food red 1, orange yellow 1, acid red 50, patent blue V, erythrin, acid orange 2, acid orange 8 and bright blue G in candied fruit was established by high-performance liquid chromatography. The sample was extracted by methanol/ammonia solution, purified by mixed weak anion reversed phase solid phase extraction, eluted by methanol/20 mmol/L ammonium acetate as mobile phase, detected by multi-wavelength diode array detector, and quantified by external standard method. The correlation coefficients were higher than 0.999 in the linear range of 0.1~20 μg/mL. The recoveries were 84.0%~104.5%, the relative standard deviations (RSDs) were 1.3%~5.3%. Results showed that, the method had the advantages of convenient operation, good stability and high recovery, and was suitable for simultaneous analysis of 20 synthetic colorants in candied fruit. The method could be extended to the analysis of other colorants with the same structure property, and could be reference significance for the detection of colorants in other food categories. |
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