Polymeric micelles based on hyaluronic acid and phospholipids: Design,characterization, and cytotoxicity |
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| Authors: | Ebrahim Saadat Mohsen Amini Rassoul Dinarvand Farid A. Dorkoosh |
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| Affiliation: | 1. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Novel amphiphilic copolymers were synthesized and characterized by 1H NMR using Hyaluronic acid (HA) as a hydrophilic part and phosphatidylethanolamine (PE) including 1,2‐dimiristoyl‐sn‐glycerol‐3‐phosphatidylethanolamine (DMPE) and 1,2‐distearoyl‐sn‐glycerol‐3‐phosphatidylethanolamine (DSPE) as a hydrophobic segment. The newly developed HA‐PE copolymers form a micelle in an aqueous media. The micellar properties, including critical micelle concentration (CMC) with pyrene as a fluorescence probe and micelle morphology, using transmission electron microscopy were assessed. It was found that the CMC values for HA‐DMPE and HA‐DSPE were 15.5 and 13.4 μg/mL, respectively. Also micelles were spherical in shape and within the size range of 162–214 nm. The solubility of cholesterol, a highly hydrophobic compound, was enhanced to 0.25 mg/mL which is much higher than it is in water (0.0001 mg/mL). In vitro cytotoxicity assay of HA‐PE copolymers showed no toxicity on human breast cancer cell line (MCF‐7). These results suggest that HA‐PE micelles could be considered as a promising carrier for delivery of hydrophobic compounds. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40944. |
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| Keywords: | biocompatibility copolymers micelles self‐assembly |
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