Highly Selective Stable Isotope Labeling of Histidine Residues by Using a Novel Precursor in E. coli‐Based Overexpression Systems |
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| Authors: | Julia Schörghuber Dr. Leonhard Geist Dr. Gerald Platzer Prof. Dr. Robert Konrat Dr. Roman J. Lichtenecker |
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| Affiliation: | 1. Institute of Organic Chemistry, University of Vienna, Vienna, Austria;2. Christian Doppler Laboratory for High-Content Structural Biology, and Biotechnology/Department of Structural and Computational Biology, University of Vienna, Vienna, Austria |
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| Abstract: | The importance of NMR spectroscopy in unraveling the structural and dynamic properties of proteins is ever‐expanding owing to progress in experimental techniques, hardware development, and novel labeling approaches. Multiple sophisticated methods of aliphatic residue labeling can be found in the literature, whereas the selective incorporation of NMR active isotopes into other amino acids still holds the potential for improvement. In order to close this methodological gap, we present a novel metabolic precursor for cell‐based protein overexpression to assemble 13C/2H isotope patterns in the peptide backbone, as well as in side chain positions of a mechanistically distinguished histidine residue. |
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| Keywords: | histidine imidazole isotope labeling NMR spectroscopy protein expression |
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