共查询到20条相似文献,搜索用时 15 毫秒
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Dr. Hui Cai Björn Palitzsch Dr. Sebastian Hartmann Natascha Stergiou Prof. Dr. Horst Kunz Prof. Dr. Edgar Schmitt Dr. Ulrika Westerlind 《Chembiochem : a European journal of chemical biology》2015,16(6):959-967
Mucin glycoproteins are important diagnostic and therapeutic targets for cancer treatment. Although several strategies have been developed to explore anti‐tumor vaccines based on MUC1 glycopeptides, only few studies have focused on vaccines directed against the tumor‐associated MUC4 glycoprotein. MUC4 is an important tumor marker overexpressed in lung cancer and uniquely expressed in pancreatic ductual adenocarcinoma. The aberrant glycosylation of MUC4 in tumor cells results in an exposure of its peptide backbone and the formation of tumor‐associated glycopeptide antigens. Due to the low immunogenicity of these endogenous structures, their conjugation with immune stimulating peptide or protein carriers are required. In this study, MUC4 tandem‐repeat glycopeptides were conjugated to the tetanus toxoid and used for vaccination of mice. Immunological evaluations showed that our MUC4‐based vaccines induced very strong antigen‐specific immune responses. In addition, antibody binding epitope analysis on glycopeptide microarrays, were demonstrating a clear glycosylation site dependence of the induced antibodies. 相似文献
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A Synthetic MUC1 Glycopeptide Bearing βGalNAc‐Thr as a Tn Antigen Isomer Induces the Production of Antibodies against Tumor Cells 下载免费PDF全文
Dr. Vanessa Leiria Campo Thalita B. Riul Leandro Oliveira Bortot Maristela B. Martins‐Teixeira Marcelo Fiori Marchiori Emanuela Iaccarino Dr. Menotti Ruvo Prof. Dr. Marcelo Dias‐Baruffi Prof. Dr. Ivone Carvalho 《Chembiochem : a European journal of chemical biology》2017,18(6):527-538
This study presents the synthesis of the novel protected O‐glycosylated amino acid derivatives 1 and 2 , containing βGalNAc‐SerOBn and βGalNAc‐ThrOBn units, respectively, as mimetics of the natural Tn antigen (αGalNAc‐Ser/Thr), along with the solid‐phase assembly of the glycopeptides NHAcSer‐Ala‐Pro‐Asp‐Thr[αGalNAc]‐Arg‐Pro‐Ala‐Pro‐Gly‐BSA ( 3 ‐BSA) and NHAcSer‐Ala‐Pro‐Asp‐Thr[βGalNAc]‐Arg‐Pro‐Ala‐Pro‐Gly‐BSA ( 4 ‐BSA), bearing αGalNAc‐Thr or βGalNAc‐Thr units, respectively, as mimetics of MUC1 tumor mucin glycoproteins. According to ELISA tests, immunizations of mice with βGalNAc‐glycopeptide 4 ‐BSA induced higher sera titers (1:320 000) than immunizations with αGalNAc‐glycopeptide 3 ‐BSA (1:40 000). Likewise, flow cytometry assays showed higher capacity of the obtained anti‐glycopeptide 4 ‐BSA antibodies to recognize MCF‐7 tumor cells. Cross‐recognition between immunopurified anti‐βGalNAc antibodies and αGalNAc‐glycopeptide and vice versa was also verified. Lastly, molecular dynamics simulations and surface plasmon resonance (SPR) showed that βGalNAc‐glycopeptide 4 can interact with a model antitumor monoclonal antibody (SM3). Taken together, these data highlight the improved immunogenicity of the unnatural glycopeptide 4 ‐BSA, bearing βGalNAc‐Thr as Tn antigen isomer. 相似文献
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Inside Cover: Synthesis of Cholesterol‐Substituted Glycopeptides for Tailor‐Made Glycocalyxification of Artificial Membrane Systems (ChemBioChem 15/2016) 下载免费PDF全文
Dr. Nicolai Stuhr‐Hansen Dr. Josef Madl Sarah Villringer Dr. Ulrika Aili Prof. Dr. Winfried Römer Dr. Ola Blixt 《Chembiochem : a European journal of chemical biology》2016,17(15):1386-1386
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Inside Cover: Microdomain Formation Controls Spatiotemporal Dynamics of Cell‐Surface Glycoproteins (ChemBioChem 14/2015) 下载免费PDF全文
Leonhard Möckl Andrea K. Horst Katharina Kolbe Prof. Dr. Thisbe K. Lindhorst Prof. Dr. Christoph Bräuchle 《Chembiochem : a European journal of chemical biology》2015,16(14):1966-1966
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Inside Back Cover: Expanding the Imine Reductase Toolbox by Exploring the Bacterial Protein‐Sequence Space (ChemBioChem 12/2015) 下载免费PDF全文
Dr. Dennis Wetzl Dr. Marco Berrera Dr. Nicolas Sandon Dr. Dan Fishlock Dr. Martin Ebeling Prof. Dr. Michael Müller Dr. Steven Hanlon Dr. Beat Wirz Dr. Hans Iding 《Chembiochem : a European journal of chemical biology》2015,16(12):1823-1823
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Inside Cover: Biological Evaluation of Multivalent Lewis X–MGL‐1 Interactions (ChemBioChem 6/2014) 下载免费PDF全文
Dr. Magdalena Eriksson Dr. Sonia Serna Dr. Maha Maglinao Dr. Mark K. Schlegel Prof. Dr. Peter H. Seeberger Dr. Niels‐Christian Reichardt Dr. Bernd Lepenies 《Chembiochem : a European journal of chemical biology》2014,15(6):770-770
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Back Cover: Directed Divergent Evolution of a Thermostable D‐Tagatose Epimerase towards Improved Activity for Two Hexose Substrates (ChemBioChem 4/2015) 下载免费PDF全文
Andreas Bosshart Dr. Chee Seng Hee Dr. Matthias Bechtold Prof. Tilman Schirmer Prof. Sven Panke 《Chembiochem : a European journal of chemical biology》2015,16(4):696-696