高效液相色谱-荧光法测定红酒中的黄酮

实验建立了高效液相色谱法(HPLC)测定红酒中的槲皮素,桑色素和山奈酚的含量的方法。采用Polaris C18(2.0×100mm,3μm)色谱柱,柱温为60℃,流动相:A相0.1mol/LHAc,B相为乙腈,流速为0.2 mL/min,梯度淋洗,柱后衍生,激发波长438nm,发射波长483nm。结果:黄酮的线性范围为0.25mg/L~4.0mg/L。平均加样回收率89.7%~95.1%,相对标准偏差为1.37%~-2.98%。该方法简便、准确,可用于天然产物中槲皮素,桑色素和山奈酚含量的测定。     

RP-HPLC测定小茴香中槲皮素和山奈酚含量

建立用反相高效液相色谱法同时测定小茴香中槲皮素和山奈酚含量的方法。采用Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm);柱温:25℃;流动相:甲醇-0.4%磷酸(50∶50);流速:1mL/min;检测波长:360nm。槲皮素、山奈酚分别在2.19~109.6μg/mL和2.28~114.0μg/mL范围内线性关系良好。槲皮素和山奈酚的平均回收率分别为95.61%,94.41%,RSD分别为2.4%,3.0%。此方法简便、准确、可靠,可作为小茴香中槲皮素和山奈酚的定量分析方法。测定出小茴香总黄酮水解物中槲皮素和山奈酚的含量分别为:槲皮素0.29 mg/g,n=3,RSD=2.60%;山奈酚含量为:0.36mg/g,n=3,RSD=3.25%。     

HPLC-UV测定红酒中7种黄酮苷元及其生物活性物

建立了三元梯度的高效液相色谱法(HPLC)测定红酒中的桑色素、槲皮素,山奈酚、异鼠李素、木犀草素、芹菜素等苷元的含量的方法。采用Luna C18(4.6mm×150mm,3μm)色谱柱,预柱(4mm×3.0mm),柱温为50℃,流动相:A相乙腈,B相乙醇,C相为0.2%磷酸水溶液。流速为0.25mL/min,梯度淋洗,检测波长265nm。结果显示:各黄酮苷元在0.7mg/L~32.0mg/L呈线性关系,样品的加标平均回收率为98.2%~101.1%,RSD为1.88%~2.85%,该方法简便、准确,可用于天然产物中黄酮苷元的含量的测定。     

非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚

目的：以5g/100mL非离子表面活性剂Triton X-100为萃取剂,建立一种简单快速的采用超声提取,高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚的方法。方法：采用Phenomenex C18色谱柱(250mm×4.6mm,5.0μm),以甲醇-0.25%乙酸溶液为流动相梯度洗脱,流速1.0mL/min,紫外检测波长350nm。结果：绿原酸、槲皮素和山奈酚分别在0.011～220μg/mL(r＝0.9997)、0.065～130μg/mL(r＝0.9999)、0.068～136μg/mL(r＝0.9999)线性关系良好,检出限(RSN＝3)依次是1.94、0.22、0.27ng/mL;样品的加标回收率为90.40%～96.82%。结论：该方法具有样品前处理简单、绿色环保、灵敏快速等优点,可为枇杷叶中一些黄酮类物质和绿原酸的检测提供一个有效的方法。     

不同秋果型树莓中槲皮素和山奈酚含量的测定

通过高效液相色谱法测定,不同秋果型树莓中槲皮素和山奈酚的含量。高效液相色谱法的测定条件:色谱柱为安捷伦18反相色谱柱(250 mm×4.6 mm,5μm),甲醇-1%乙酸溶液为流动相,流速为1.000 mL/min,测定波长254 nm,进样量10μL。结果表明:槲皮素和山奈酚分别在0.11μg/mL～11μg/mL(R~2=0.999 8);0.11μg/mL～27.5μg/mL(R~2=0.999 7)呈良好的线性关系;加样回收率分别为为0.76%、0.91%。     

HPLC双波长法测定豌豆尖中槲皮素和山奈酚的含量

目的:建立HPLC双波长法同时测定豌豆尖中类黄酮水解产物槲皮素和山奈酚含量的方法。方法:先用甲醇-15%盐酸(4:1,v/v)混合液把豌豆尖中的类黄酮水解成槲皮素和山奈酚,后采用HPLC双波长法测定槲皮素和山奈酚的含量。GRACE Vision HT C_(18)色谱柱(250 mm×4.6mm,5μm),流动相为甲醇-0.1%冰醋酸(50:50,v/v),流速为1.0 mL/min,槲皮素检测波长为371 nm,山奈酚检测波长为366 nm,柱温为30℃。结果:槲皮素和山奈酚分别在(0.05~0.3)μg、(0.025~0.15)μg范围内线性关系良好,平均加标回收率分别为99.30%(RSD=1.74%)、98.33%(RSD=1.21%)。结论:该方法操作简便、结果可靠、重现性好,可用于豌豆尖中槲皮素和山奈酚含量的同时测定。     

高效液相色谱-二极管阵列检测法同时测定不同品种巴旦杏中槲皮素、山奈酚的含量

目的:建立用高效液相色谱-二极管阵列检测法测定新疆不同品种巴旦杏仁中槲皮素、山奈酚含量的方法。方法:色谱柱:HC-C18柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.4%磷酸溶液(55∶45,V/V);检测波长:360 nm;柱温:30℃;流速:1.00 mL/min。结果:槲皮素和山奈酚分别在1.0~30μg/mL(r=0.999 3)和0.5~40μg/mL(r=0.999 8)质量浓度范围内呈良好的线性关系,平均回收率分别为102.0%(相对标准偏差为2.08%,n=5)和102.8%(相对标准偏差为2.21%,n=5)。结论:该方法简便、快速、有效、准确、具有良好的重复性和回收率,可作为巴旦杏仁的质量控制和评价。     

UPLC测定豌豆尖中槲皮素和山奈酚的含量

目的:采用超高效液相色谱法(UPLC)测定豌豆尖中类黄酮水解产物槲皮素和山奈酚的含量。方法:采用Waters ACQUITY UPLC BEH Shield RP18色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.1%冰醋酸水溶液(60:40)为流动相,流速为0.4 m L/min,槲皮素检测波长为371 nm,山奈酚检测波长为366 nm,柱温为30℃,进样量2.0μL。结果:槲皮素和山奈酚在1.5 min时间内达到基线分离,分别在(0.03~0.18)mg/m L、(0.015~0.15)mg/m L范围内与峰面积线性关系良好,平均回收率分别为101.37%、100.64%,RSD分别为0.84%、0.37%。结论:该方法快速、准确、稳定,可用于豌豆尖中槲皮素和山奈酚的含量测定。     

HPLC法同时检测5种龙胆草黄酮类物质

比较不同来源龙胆草提取物对芦丁、槲皮素、木犀草素、山奈酚、异鼠李素5种黄酮物质的高效液相色谱法检测及龙胆草来源对其黄酮物质含量的影响。试验采用REPROSIL-PUR BASIC C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相A相为0.30%乙酸水溶液;流动相B相为85%甲醇水溶液,梯度洗脱,流速1.0 mL/min,VWD检测波长283 nm;柱温40℃。检测方法显示,5种黄酮物质的检测线性范围为0.02~200 mg/L;检出限(S/N=3)在0.003~0.050mg/kg之间,加标回收率(N=6)在86.03%~105.39%之间,检测方法灵敏有效。检测结果表明,不同来源龙胆草提取物得到的龙胆草提取物中芦丁、槲皮素、木犀草素、山奈酚、异鼠李素5种龙胆草提取物黄酮物质的总含量以内蒙古呼伦贝尔产龙胆草提取物最高。     

蒲公英中黄酮类物质的快速检测

比较不同来源蒲公英提取物对芦丁、槲皮素、木犀草素、山奈酚、异鼠李素5种黄酮物质的高效液相色谱法检测及蒲公英来源对其黄酮物质含量的影响。采用BREEZE-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相A为0.25%乙酸水溶液;流动相B为90%甲醇水溶液,梯度洗脱,流速0.75 mL/min,DAD检测波长283 nm;柱温40℃。检测方法显示,5种黄酮物质的检测线性范围为0.02~500 mg/L;检出限(S/N=3)在0.002~0.04 mg/kg;在加标浓度10.0,20.0和50.0 mg/kg时,加标回收率(n=6)在86.25%~107.10%,检测方法灵敏有效。检测结果表明,不同来源蒲公英得到的蒲公英提取物中芦丁、槲皮素、木犀草素、山奈酚、异鼠李素5种蒲公英提取物黄酮物质总含量以江苏徐州产蒲公英提取物最高。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the