电泳法在食品过敏原检测中的应用

本文简要介绍了食品过敏原的检测特点和难点,比较了目前常用的聚合酶链式反应法、环介导等温扩增法、酶联免疫法、液相色谱法和液相色谱质谱法等检测方法的优劣势。主要介绍了电泳技术的特点,总结了经典电泳技术在食品过敏原分析上的应用现状。详细介绍了近年来毛细管电泳技术在食品过敏原检测研究方面取得的进展,列举了区带毛细管电泳法、亲和毛细管电泳法、凝胶毛细管电泳法、动态涂层毛细管电泳法和芯片毛细管电泳法在致敏蛋白分析方面的应用,并对电泳法在食品过敏原分析中的发展趋势进行了展望。     

毛细管电泳在食品分析中的应用

介绍了毛细管电泳 (CE)在食品分析中的应用。重点介绍了食品中无机阳离子、无机阴离子、有机酸、碳水化合物、维生素、脂肪酸、氨基酸、食品添加剂及农药残留等成分的 CE分析方法。并就毛细管电泳法的检出下限、线性和精密度 ,与其它方法作了比较。     

毛细管电泳在蛋白质及其水解产物分析中的应用及进展

蛋白质是人体所有细胞和组织的重要成分,由氨基酸通过脱水缩合组成的多肽链经过盘曲折叠而构成。由于蛋白种类多且结构复杂,传统方法很难对其进行分离分析,电泳方法由此诞生。毛细管电泳是一种将电泳和色谱有机结合的快速分离技术。本文针对食品和药品领域,对毛细管电泳在蛋白质及其水解产物分析中的最新应用及研究进展进行了综述,主要从毛细管电泳的基本原理及主要类型、毛细管电泳与其他技术的联用及应用现状、毛细管电泳在食品和药品中蛋白质及其水解产物的分析应用方面进行了阐述和总结,并展望了毛细管电泳技术在特殊医学用途配方食品中蛋白质和多肽分子量测定方面的发展前景,以期为特殊医学用途配方食品中整蛋白及多肽分子量分布测定方法的开发提供参考。     

毛细管电泳在分析食品中农药残留的应用

人类食用被农药污染的食品后,其中的农药会贮存在体内,造成人的急性或慢性中毒。世界各国对农残问题高度重视,积极研发快速、可靠、灵敏的食品农药残留分析方法。毛细管电泳是近年发展起来的一种新型的分析技术,文中介绍了毛细管电泳在分析食品中农药残留的应用。     

毛细管电泳涂层研究及食品检测中的应用

毛细管电泳涂层技术因为分离碱性蛋白的需求而诞生,研究者致力于采用不同的材料和方法提高毛细管电泳的分离性能。目前,对毛细管内壁进行涂层改性依然是提高毛细管电泳的分离效果和重现性,抑制分析物与毛细管内壁间吸附作用的最有效、最常用的方法。随着毛细管电泳(CE)与质谱(MS),激光诱导荧光检测(LIF)等联用技术的发展,毛细管电泳涂层在生命科学技术、生物医药工程、环境卫生保护、食品质量检测等领域的广泛应用。本文根据涂层材料的种类和制备机理,对近年来毛细管电泳涂层技术的特点、发展和在食品检测领域的应用状况进行了综述。     

毛细管电泳在食品分析中的应用

论述毛细管电泳在食品分析中的应用,主要包括蛋白质,糖类,维生素,矿物质,有机酸,食品添加剂,农药残留量,生物毒素,抗生素残留量和食品中其它一些物质的测定。     

痕量食品外源药毛细管电泳检测方法研究进展

介绍毛细管电泳两种分离模式的基本原理和特点。论述毛细管电泳在线富集技术的优点及胶束电动毛细管色谱在线堆积、推扫富集技术检测原理。综述两种分离模式、两种在线富集技术在食品痕量外源药检测中的研究进展,并对毛细管电泳在线富集技术应用前景进行展望。     

毛细管电泳在食品添加剂检测中的应用

作者对毛细管电泳仪的工作原理、分离模式等内容进行了简要的介绍,综述了近年来毛细管电泳在食品添加剂检测中的应用,主要包括防腐剂、甜味剂、酸味剂、色素以及营养强化剂等的测定,展望了毛细管电泳技术在食品研究、开发中的应用前景。     

食品中生物胺检测方法及控制技术的研究进展

生物胺是广泛存在于各种食品中的一大类化合物,过量的生物胺存在危害性。本文首先简单阐述了食品中生物胺的存在情况,在此基础上对最新的、常用的生物胺检测方法高效液相色谱法、毛细管电泳法、气相色谱法等进行了分析比较,提出一些控制生物胺生成的技术及措施,同时对食品中生物胺的研究前景进行展望。     

食品中生物胺检测方法的研究进展

简述食品中生物胺的种类和产生形式,主要介绍液相色谱、气相色谱、薄层色谱、传感器和毛细管电泳等方法在生物胺检测中的应用研究进展,并展望了生物胺检测方法的发展方向。     

Pressure-assisted capillary electrophoresis mass spectrometry using combination of polarity reversion and electroosmotic flow for metabolomics anion analysis

We have developed a method based on capillary electrophoresis (CE) coupled to mass spectrometry (MS) that is a powerful tool for metabolome analysis. In this paper, a simple method for the simultaneous analysis of anionic metabolites such as sugar phosphates, organic acids, nucleotides and CoA compounds based on pressure-assisted capillary electrophoresis coupled to electrospray ionization mass spectrometry (PACE/ESI-MS) is described. The feature of this method is that CE polarity, where the inlet of the capillary is at the anode and the outlet at the cathode, is inverted from conventional CE analysis for anions. Moreover, an ordinary fused silica capillary was chosen instead of a specific cationic polymer-coated capillary (SMILE (+) capillary). A robust and inexpensive analytical method was established from the above-mentioned CE polarity and noncoated fused silica capillary. A trimethylamine acetate electrolyte, pH 10.0, provides a high resolution between isomers. Electrolyte flow using an air pump after electrophoresis enables the comprehensive and simultaneous analyses of sugar phosphates, organic acids, nucleotides and a CoA compound that to date have been impossible to analyze. Our method can be used as a de facto standard for metabolome analysis.     

植物乳杆菌6003对肌肉蛋白质的分解能力(Ⅰ)

本文研究了植物乳杆菌6003活细胞(Cell)、细胞裂解液(Cellextracts,CE)以及两者混合液(Cell+CE)对肌浆提取液中的蛋白质的分解作用。通过蛋白质、肽、游离氨基酸分析,表明Cell组以及Cell+CE组对肌浆提取液中蛋白质、肽分解和游离氨基酸的增加均有一定作用,其中Cell+CE组中这一现象更为显著。CE组对肌浆蛋白质的分解不起作用。     

植物乳杆菌6003对肌肉蛋白质的分解能力(Ⅱ)

本文研究了植物乳杆菌6003活细胞(Cell)、细胞裂解液(Cell extracs，CE)以及两者混合液(Cell CE)对肌原纤维提取液中的蛋白质的分解作用。通过蛋白质、肽、游离氨基酸分析，表明各实验组对肌原纤维提取液中蛋白、肽分解、游离氨基酸的增加作用较弱，其中Cell CE组中作用稍为显著，CE组对肌原纤维提取液中蛋白质的分解几乎不起作用。     

浊点萃取技术及其在食品检测预处理中的应用

浊点萃取技术是一种基于表面活性剂水溶液中相分离现象的环保新型液-液萃取技术,并实现了与HPLC、CE、FI等多种分析仪器的联用。介绍了其原理、操作流程、影响因素以及在食品检测预处理中的应用。     

脂肪酸检测在食品工业中的应用研究进展

脂肪酸是油脂的重要组成成分,不同食品脂肪酸组成与含量存在差异性,通过脂肪酸分析可以溯源产地、区分品种、分析品质优劣、开发高质量产品。文章论述了脂肪酸检测在肉品、乳与乳制品、植物油、药食同源食材中的应用。     

油脂加工减控反式脂肪酸技术研究进展

该文简介反式脂肪酸性质、来源、分类、对人体健康主要危害及世界各国管理规定,分析油脂加工过程中反式脂肪酸生成机理、途径及影响其生成工艺技术参数,重点综述阻断或减少油脂加工过程中反式脂肪酸生成途径与方法,并提出减控反式脂肪酸生成技术研究、反式脂肪酸安全风险分析与控制、监督管理措施等方面建议。     

Two-step mass spectrometric approach for the identification of diketopiperazines in chicken essence

Diketopiperazines (DKPs) have been found in a variety of foods and beverages that impart a metallic bitter taste. The presence of DKPs in chicken essence (CE) was first postulated by a two-step monitoring technique, with mass precursor ion scanning from the immonium ion of a specific amino acid first, followed by mass matching with a DKP database. The postulated DKPs were further verified by tandem mass spectrometric (MS/MS) analysis. It was found that this technique performed well, with about 20 DKPs tentatively identified. Proline was an important constituent of the DKPs in CE, together with other non-polar amino acids, such as glycine, alanine, valine, leucine/isoleucine and phenylalanine, which also showed great magnitude. Some proline-based DKPs in CE were quantified before and after thermal treatment at 130 °C for 1 h. The concentrations of the DKPs were determined not only by the relative ease of formation and the stability of the DKPs, but also by past thermal processings.     

Associations of cytological endometritis with energy metabolism and inflammation during the periparturient period and early lactation in dairy cows

Multiparous Holstein cows (n = 108) were used to determine the associations of cytological endometritis (CE) with plasma nonesterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) as markers of energy metabolism, calculated energy balance (EB), and plasma haptoglobin (Hp) as a marker of inflammation during the periparturient period and early lactation. Evaluation of endometrial cytology by low-volume uterine lavage was conducted on 1 d between 40 and 60 d postcalving. The incidence of CE among cows sampled was 40%. The area under the curve (AUC) was calculated for both NEFA and BHBA using data collected from 3 wk before to 3 wk after parturition. Data for NEFA and BHBA AUC were stratified into prepartum (wk −3 to parturition) and postpartum (parturition to wk +3) for statistical analysis. Prepartum AUC for neither NEFA nor BHBA was associated with subsequent CE; however, cows that subsequently developed CE tended to have higher postpartum AUC for NEFA and had higher postpartum AUC for BHBA. Consistent with the results for plasma NEFA and BHBA, calculated EB during the prepartum period was not different in cows that did or did not develop CE; however, cows with CE had lower EB during the 6-wk postpartum period compared with cows without CE. Analysis of EB by week (wk −3 to −1 before calving and wk +1 to +6 postcalving) indicated that EB in cows with CE was lower at wk +1, +2, and +3 and tended to be lower at wk +6 than cows without CE. Plasma Hp concentrations were analyzed from wk +1 to +8 of lactation; concentrations of Hp were not different during either wk +1 or the entire postpartum period between cows that did or did not develop CE. These results suggest that lower energy status during the first 3 wk postpartum, but not necessarily systemic inflammation, is associated with subsequent development of CE.     

Influence of finishing diet on fatty acid profiles of intramuscular lipids, triglycerides and phospholipids in muscles of the Iberian pig

Thirty Iberian × Duroc pigs allotted in groups of ten animals were fed in three traditional different management systems ('Montanera' (MO), fed on acorns; 'Recebo' (RE), fed on acorns and a commercial diet; and 'Cebo' (CE), fed on a commercial diet). Masseter muscle was obtained to evaluate the influence of management system on fatty acid (FA) composition of lean. The FA composition of the intramuscular total lipids, triglyceride (TG) and phospholipid (PL) fractions was evaluated. Muscle from MO pigs had greater quantities of monounsaturated fatty acids (MUFA) in the total lipids, triglyceride and phospholipid fractions than the other feedings. The percentage of saturated fatty acids (SFA) of i.m. total lipids and TGs increased (P < .05) with duration of feeding on RE and CE, from 35.13-35.10% in MO pigs to 37.47-37.84% in RE pigs and 39.98-41.11% in CE pigs. PLs from RE and CE pigs contained more C(18:2) and C(20:4) and less C(18:1) than MO pigs.