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Christopher J. Hill Jennifer R. Fleming Masoumeh Mousavinejad Rachael Nicholson Svetomir B. Tzokov Per A. Bullough Julius Bogomolovas Mark R. Morgan Olga Mayans Patricia Murray 《Advanced materials (Deerfield Beach, Fla.)》2019,31(17)
The development of extracellular matrix mimetics that imitate niche stem cell microenvironments and support cell growth for technological applications is intensely pursued. Specifically, mimetics are sought that can enact control over the self‐renewal and directed differentiation of human pluripotent stem cells (hPSCs) for clinical use. Despite considerable progress in the field, a major impediment to the clinical translation of hPSCs is the difficulty and high cost of large‐scale cell production under xeno‐free culture conditions using current matrices. Here, a bioactive, recombinant, protein‐based polymer, termed ZTFn, is presented that closely mimics human plasma fibronectin and serves as an economical, xeno‐free, biodegradable, and functionally adaptable cell substrate. The ZTFn substrate supports with high performance the propagation and long‐term self‐renewal of human embryonic stem cells while preserving their pluripotency. The ZTFn polymer can, therefore, be proposed as an efficient and affordable replacement for fibronectin in clinical grade cell culturing. Further, it can be postulated that the ZT polymer has significant engineering potential for further orthogonal functionalization in complex cell applications. 相似文献
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Metal Ion‐Induced Self‐Assembly of a Multi‐Responsive Block Copolypeptide into Well‐Defined Nanocapsules
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Mark B. van Eldijk Lise Schoonen Jeroen J. L. M. Cornelissen Roeland J. M. Nolte Jan C. M. van Hest 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(18):2476-2483
Protein cages are an interesting class of biomaterials with potential applications in bionanotechnology. Therefore, substantial effort is spent on the development of capsule‐forming designer polypeptides with a tailor‐made assembly profile. The expanded assembly profile of a triblock copolypeptide consisting of a metal ion chelating hexahistidine‐tag, a stimulus‐responsive elastin‐like polypeptide block, and a pH‐responsive morphology‐controlling viral capsid protein is presented. The self‐assembly of this multi‐responsive protein‐based block copolymer is triggered by the addition of divalent metal ions. This assembly process yields monodisperse nanocapsules with a 20 nm diameter composed of 60 polypeptides. The well‐defined nanoparticles are the result of the emergent properties of all the blocks of the polypeptide. These results demonstrate the feasibility of hexahistidine‐tags to function as supramolecular cross‐linkers. Furthermore, their potential for the metal ion‐mediated encapsulation of hexahistidine‐tagged proteins is shown. 相似文献
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Protein Delivery: Bioinspired Diselenide‐Bridged Mesoporous Silica Nanoparticles for Dual‐Responsive Protein Delivery (Adv. Mater. 29/2018)
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Dan Shao Mingqiang Li Zheng Wang Xiao Zheng Yeh‐Hsing Lao Zhimin Chang Fan Zhang Mengmeng Lu Juan Yue Hanze Hu Huize Yan Li Chen Wen‐fei Dong Kam W. Leong 《Advanced materials (Deerfield Beach, Fla.)》2018,30(29)
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WO3 Nanofiber‐Based Biomarker Detectors Enabled by Protein‐Encapsulated Catalyst Self‐Assembled on Polystyrene Colloid Templates
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Seon‐Jin Choi Sang‐Joon Kim Hee‐Jin Cho Ji‐Soo Jang Yi‐Min Lin Harry L. Tuller Gregory C. Rutledge Il‐Doo Kim 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(7):911-920
A novel catalyst functionalization method, based on protein‐encapsulated metallic nanoparticles (NPs) and their self‐assembly on polystyrene (PS) colloid templates, is used to form catalyst‐loaded porous WO3 nanofibers (NFs). The metallic NPs, composed of Au, Pd, or Pt, are encapsulated within a protein cage, i.e., apoferritin, to form unagglomerated monodispersed particles with diameters of less than 5 nm. The catalytic NPs maintain their nanoscale size, even following high‐temperature heat‐treatment during synthesis, which is attributed to the discrete self‐assembly of NPs on PS colloid templates. In addition, the PS templates generate open pores on the electrospun WO3 NFs, facilitating gas molecule transport into the sensing layers and promoting active surface reactions. As a result, the Au and Pd NP‐loaded porous WO3 NFs show superior sensitivity toward hydrogen sulfide, as evidenced by responses (Rair/Rgas) of 11.1 and 43.5 at 350 °C, respectively. These responses represent 1.8‐ and 7.1‐fold improvements compared to that of dense WO3 NFs (Rair/Rgas = 6.1). Moreover, Pt NP‐loaded porous WO3 NFs exhibit high acetone sensitivity with response of 28.9. These results demonstrate a novel catalyst loading method, in which small NPs are well‐dispersed within the pores of WO3 NFs, that is applicable to high sensitivity breath sensors. 相似文献
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SnO2 Nanoparticles: Graphene‐Skeleton Heat‐Coordinated and Nanoamorphous‐Surface‐State Controlled Pseudo‐Negative‐Photoconductivity of Tiny SnO2 Nanoparticles (Adv. Mater. 23/2015)
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Longwei Ding Nishuang Liu Luying Li Xing Wei Xianghui Zhang Jun Su Jiangyu Rao Congxing Yang Wenzhi Li Jianbo Wang Haoshuang Gu Yihua Gao 《Advanced materials (Deerfield Beach, Fla.)》2015,27(23):3579-3579
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Gary A. Dilts 《International journal for numerical methods in engineering》1999,44(8):1115-1155
The Smooth‐Particle‐Hydrodynamics (SPH) method is derived in a novel manner by means of a Galerkin approximation applied to the Lagrangian equations of continuum mechanics as in the finite‐element method. This derivation is modified to replace the SPH interpolant with the Moving‐Least‐Squares (MLS) interpolant of Lancaster and Saulkaskas, and define a new particle volume which ensures thermodynamic compatibility. A variable‐rank modification of the MLS interpolants which retains their desirable summation properties is introduced to remove the singularities that occur when divergent flow reduces the number of neighbours of a particle to less than the minimum required. A surprise benefit of the Galerkin SPH derivation is a theoretical justification of a common ad hoc technique for variable‐h SPH. The new MLSPH method is conservative if an anti‐symmetric quadrature rule for the stiffness matrix elements can be supplied. In this paper, a simple one‐point collocation rule is used to retain similarity with SPH, leading to a non‐conservative method. Several examples document how MLSPH renders dramatic improvements due to the linear consistency of its gradients on three canonical difficulties of the SPH method: spurious boundary effects, erroneous rates of strain and rotation and tension instability. Two of these examples are non‐linear Lagrangian patch tests with analytic solutions with which MLSPH agrees almost exactly. The examples also show that MLSPH is not absolutely stable if the problems are run to very long times. A linear stability analysis explains both why it is more stable than SPH and not yet absolutely stable and an argument is made that for realistic dynamic problems MLSPH is stable enough. The notion of coherent particles, for which the numerical stability is identical to the physical stability, is introduced. The new method is easily retrofitted into a generic SPH code and some observations on performance are made. Copyright © 1999 John Wiley & Sons, Ltd. 相似文献
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Bioinspired Diselenide‐Bridged Mesoporous Silica Nanoparticles for Dual‐Responsive Protein Delivery
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Dan Shao Mingqiang Li Zheng Wang Xiao Zheng Yeh‐Hsing Lao Zhimin Chang Fan Zhang Mengmeng Lu Juan Yue Hanze Hu Huize Yan Li Chen Wen‐fei Dong Kam W. Leong 《Advanced materials (Deerfield Beach, Fla.)》2018,30(29)
Controlled delivery of protein therapeutics remains a challenge. Here, the inclusion of diselenide‐bond‐containing organosilica moieties into the framework of silica to fabricate biodegradable mesoporous silica nanoparticles (MSNs) with oxidative and redox dual‐responsiveness is reported. These diselenide‐bridged MSNs can encapsulate cytotoxic RNase A into the 8–10 nm internal pores via electrostatic interaction and release the payload via a matrix‐degradation controlled mechanism upon exposure to oxidative or redox conditions. After surface cloaking with cancer‐cell‐derived membrane fragments, these bioinspired RNase A‐loaded MSNs exhibit homologous targeting and immune‐invasion characteristics inherited from the source cancer cells. The efficient in vitro and in vivo anti‐cancer performance, which includes increased blood circulation time and enhanced tumor accumulation along with low toxicity, suggests that these cell‐membrane‐coated, dual‐responsive degradable MSNs represent a promising platform for the delivery of bio‐macromolecules such as protein and nucleic acid therapeutics. 相似文献
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Protein‐Encapsulated Catalysts: WO3 Nanofiber‐Based Biomarker Detectors Enabled by Protein‐Encapsulated Catalyst Self‐Assembled on Polystyrene Colloid Templates (Small 7/2016)
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Seon‐Jin Choi Sang‐Joon Kim Hee‐Jin Cho Ji‐Soo Jang Yi‐Min Lin Harry L. Tuller Gregory C. Rutledge Il‐Doo Kim 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(7):964-964
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Bio‐Nanostructures: Protein Bricks: 2D and 3D Bio‐Nanostructures with Shape and Function on Demand (Adv. Mater. 20/2018)
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Jianjuan Jiang Shaoqing Zhang Zhigang Qian Nan Qin Wenwen Song Long Sun Zhitao Zhou Zhifeng Shi Liang Chen Xinxin Li Ying Mao David L. Kaplan Stephanie N. Gilbert Corder Xinzhong Chen Mengkun Liu Fiorenzo G. Omenetto Xiaoxia Xia Tiger H. Tao 《Advanced materials (Deerfield Beach, Fla.)》2018,30(20)
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One‐Pot Synthesis of Multiple Protein‐Encapsulated DNA Flowers and Their Application in Intracellular Protein Delivery
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Eunjung Kim Limor Zwi‐Dantsis Natalie Reznikov Catherine S. Hansel Shweta Agarwal Molly M. Stevens 《Advanced materials (Deerfield Beach, Fla.)》2017,29(26)
Inspired by biological systems, many biomimetic methods suggest fabrication of functional materials with unique physicochemical properties. Such methods frequently generate organic–inorganic composites that feature highly ordered hierarchical structures with intriguing properties, distinct from their individual components. A striking example is that of DNA–inorganic hybrid micro/nanostructures, fabricated by the rolling circle technique. Here, a novel concept for the encapsulation of bioactive proteins in DNA flowers (DNF) while maintaining the activity of protein payloads is reported. A wide range of proteins, including enzymes, can be simultaneously associated with the growing DNA strands and Mg2PPi crystals during the rolling circle process, ultimately leading to the direct immobilization of proteins into DNF. The unique porous structure of this construct, along with the abundance of Mg ions and DNA molecules present, provides many interaction sites for proteins, enabling high loading efficiency and enhanced stability. Further, as a proof of concept, it is demonstrated that the DNF can deliver payloads of cytotoxic protein (i.e., RNase A) to the cells without a loss in its biological function and structural integrity, resulting in highly increased cell death compared to the free protein. 相似文献