共查询到20条相似文献，搜索用时 0 毫秒
1.
Alice Pranzetti Stéphanie Salaün Sophie Mieszkin Maureen E. Callow James A. Callow Jon A. Preece Paula M. Mendes 《Advanced functional materials》2012,22(17):3672-3681
Understanding how bacteria adhere to a surface is a critical step in the development of novel materials and coatings to prevent bacteria forming biofilms. Here, surface plasmon resonance (SPR) spectroscopy, in combination with self‐assembled monolayers (SAMs) that have different backbone structures and/or functional groups, is used for the first time to study the initial stages of bacterial adhesion to surfaces (i.e., initial interaction of cells with a surface, a process governed by van der Waals, electrostatic, and hydrophobic interactions). The work highlights SPR spectroscopy as a powerful and unique approach to probe bacterial adhesion in real time. SPR spectral data reveal different kinetics of adhesion for the interaction of two marine bacterial species (Marinobacter hydrocarbonoclasticus and Cobetia marina) to a range of organosulfur SAMs. Furthermore, the extent of adhesion is dependent on the backbone structures and functional groups of the SAMs. The role of extracellular polymeric substances (EPS) in bacterial adhesion is also investigated. Pre‐conditioning experiments with cell‐free culture supernatants, containing planktonic EPS, allow quantification of the amount adsorbed onto surfaces and directly account for the impact of EPS adsorption on bacterial adhesion in the assay. While the physicochemical characteristics of the surfaces play a significant role in determining bacterial cell adhesion for low levels of conditioning by planktonic EPS, greater levels of conditioning by EPS reduce the difference between surfaces. 相似文献
2.
Zhicheng Xu Litong Chen Liangliang Lu Ruichun Du Wencan Ma Yifeng Cai Xiaoming An Haomin Wu Qiong Luo Qiang Xu Qiuhong Zhang Xudong Jia 《Advanced functional materials》2021,31(1):2006432
Stretchable electrodes are playing important roles in the measurement of bio-electrical signals especially in wearable electronic devices. These electrodes usually adopt commercial elastomers such as polydimethylsiloxane or polystyrene-ethylene-butylene-styrene as substrates, which result in poor stability and reliability due to weak interfacial adhesion between electrodes and human skin. Here, dopamine is introduced into the hydrogen bonding based elastomer as pendent groups. The elastomer shows both mechanical strength and adhesion strength at the same time. It exhibits high stress at break (1.9 MPa) and high fracture strain (5100%). Significantly, it exhibits a high adhesive strength (≈62 kPa) and underwater adhesive strength (≈16 kPa) with epithelial tissue. Thus, a stretchable bio-interfacial electrode is fabricated by spray-coating silver nanowires on the elastic substrate, which is stretchable, self-healable, and highly adhesive and suitable for electromyogram measurement. 相似文献
3.
Pengbo Zhang Xin Zhou Ruilian Qi Panpan Gai Libing Liu Fengting Lv Shu Wang 《Advanced Electronic Materials》2019,5(8)
Low organism loading capacity and inefficient extracellular electron transport (EET) are still the bottlenecks hindering the development of bioelectrochemical systems (BESs). It is shown that cationic polythiophene derivative (PMNT) has the ability to simultaneously enhance bacteria biofilm formation, improve the bacteria viability, decrease the resistance value, and accelerate the EET process between exoelectrogen and the electrode. Shewanella oneidensis can form a robust and thick biofilm on the electrode surface in the presence of PMNT. Mediated by electron‐transporting PMNT, even bacteria far away from the electrode can transfer electrons to it. This bioelectrode is utilized as the anode to construct a microbial fuel cell, which exhibits a greatly increased maximum current density and power density and a prolonged lifetime by taking advantage of the unique properties of PMNT. Thus, cationic conductive polymers exhibit great potential as effective biofilm enhancers and electron transporters in BESs. 相似文献
4.
Huiyi Liang Yibo Du Lixin Liu Junqiu Liu Yongming Chen 《Advanced functional materials》2023,33(19):2213465
Excessive self-DNAs recognized by intracellular DNA sensors can initiate innate immunity to express disordered TNF-α or type I IFN resulting in several autoimmune diseases. Cationic polymers have been profoundly proved to alleviate the inflammatory symptoms by removing the debris of cell-free DNA (cfDNA). However, clinical applications of cationic materials have been impeded by concerns of their toxicity and the fate of cfDNA in polymer-cfDNA complex. Herein, it is showed that PEGylated polyimidazoles as a biomimetic DNase potently alleviate pathologic symptoms of self-DNA-associated rheumatoid arthritis (RA) rats and Trex1 (DNase III) deficient Aicardi-Goutiéres syndrome (AGS) mice. The mechanism studies demonstrate that the polyimidazole efficiently attacks the phosphodiester linkages of NAs and cleavages them into small pieces. As imidazole unit is a much weaker organic base that occurs in natural proteins, the polyimidazoles are less toxic to cells and tissues, as manifested by the IC50 values larger than 1000 µg mL−1. This work suggests that synthetic tailored DNase can be a new and safe therapeutic agent to treat chronic autoimmune and refractory inflammatory diseases by degradation of excessive nucleic acids. 相似文献
5.
本文采用了"多步的改进的动态分子梳"纳米操纵技术(multi-step modified dynamic molecular combing,MMDMC)实现样品制备获得不同拉伸程度单个DNA分子,利用原子力显微镜重定位成像和单分子分析,对反应前后不同拉伸程度DNA分子的DNase I酶切产生缺口情况进行统计,在一定拉伸范围内讨论了在表面上DNase I酶切反应速率变化的动力学信息.该不同拉伸程度的DNA与DNase I相互作用的表面机械生化过程对于生物分子多次反复相互作用研究体系具有代表性意义. 相似文献
6.
Zi-xuan Qin Chen-yu Wang Jiang-shan Zhang Zhang-yi Wang Yu Wei Yu-tao Li Shi-qi Dai Franklin R Tay Li-na Niu 《Advanced functional materials》2023,33(31):2211669
The advent of biotechnology has expedited the understanding of the biochemistry of deoxyribonucleic acids (DNA). In the past, DNA are thought to be present only in cell nucleus as bearers of the genetic code. With the identification of extracellular DNA in circulating body fluids, DNA are now utilized, at least experimentally, for diagnosis and treatment of diseases. Extracellular DNA of host origin trigger immune responses, and are closely linked to autoimmune disease, cancer-related inflammation, bacteria adhesion and thrombosis. Recent advancements in DNA nanotechnology have led to the development of a series of DNA-based materials for treating diseases because of their structural programmability. Current discussions on biosafety and immunogenicity of artificial DNA materials are insufficient. This issue severely restricts the clinical translation of these novel biotechnologies. The present review attempts to bridge the gap between natural extracellular DNA and their derivatives, DNA-based materials. The pathological attributes of endogenous extracellular DNA motivate the design of targeting DNA materials. In addition, the fate of exogenous DNA in the host inspires the optimization of DNA materials in reducing immune rejection. These bioinspired strategies provide the blueprint for utilizing DNA materials in the management of diseases that are currently challenging to diagnose or treat. 相似文献
7.
Sen Yang Jianpu Tang Yu Cheng Mingxing Liu Zhaoyue Lv Rui Zhang Chunzhi Zhang Chi Yao Dayong Yang 《Advanced functional materials》2024,34(44):2404900
Tumor-derived small extracellular vesicles (sEVs) are proven to play important roles in accelerating the progression of tumors. Destructing the sEVs at tumor sites is therefore a promising route to inhibit tumor progression. Herein, a smart DNA network-based sEV trap (DNET) is reported, which achieved the specific capture of tumor-derived sEVs through recognizing sEVs by polyaptamers, and the efficient destruction of sEVs via a photodynamic process at tumor sites. The DNET is constructed through the assembly of two DNA chains generated via rolling circle amplification. The DNA chains contain polyaptamers for capturing sEVs, polyvalent G-quadruplexes for loading photodynamic reagents, and complementary segments for forming a cross-linked network. Upon the irradiation of the laser, the captured sEVs in DNET are destructed, causing significant inhibition effects on the migration, invasion, and proliferation of glioblastoma cells. In particular, DNET achieved tumor inhibition rates of 39.70% in the zebrafish tumor model, and 73.10% in the nude mouse tumor model, respectively, demonstrating the significant efficacy of DNET in inhibiting tumor progression. 相似文献
8.
Jan J. T. M. Swartjes Deepak H. Veeregowda Henny C. van der Mei Henk J. Busscher Prashant K. Sharma 《Advanced functional materials》2014,24(28):4435-4441
Bacterial adhesion is problematic in many diverse applications. Coatings of hydrophilic polymer chains in a brush configuration reduce bacterial adhesion by orders of magnitude, but not to zero. Here, the mechanism by which polymer‐brush functionalized surfaces reduce bacterial adhesion from a flowing carrier fluid by relating bacterial adhesion with normally oriented adhesion and friction forces on polymer (PEG)‐brush coatings of different softness is studied. Softer brush coatings deform more than rigid ones, which yields extensive bond‐maturation and strong, normally oriented adhesion forces, accompanied by irreversible adhesion of bacteria. On rigid brushes, normally oriented adhesion forces remain small, allowing desorption and accordingly lower numbers of adhering bacteria result. Friction forces, generated by fluid flow and normally oriented adhesion forces, are required to oppose fluid shear forces and cause immobile adhesion. Summarizing, inclusion of friction forces and substratum softness provides a more complete mechanism of bacterial adhesion from flowing carrier fluids than available hitherto. 相似文献
9.
Jun Zhou Jiawei Mei Quan Liu Dongdong Xu Xiaoli Wang Xianzuo Zhang Wanbo Zhu Chen Zhu Jiaxing Wang 《Advanced functional materials》2023,33(12):2211811
Biofilm-infected diabetic wounds (BIDWs) with hyperglycemia and bacterial colonization are characterized by disordered inflammation and abnormal activation of NLRP3 inflammasome, leading to sustained macrophage M1 polarization and neutrophil extracellular traps (NETs) formation. An immoderate anti-inflammatory treatment that downregulates NLRP3 in turn promotes the persistence of biofilm infections and impairs the healing of BIDWs. Therefore, reconciling biofilm elimination and immune regulation holds the promise of curing BIDWs. Herein, a novel spatiotemporal on–off immunomodulatory therapy (SOIT) is proposed for treating BIDWs through biphasic regulation of NLRP3. Methacrylate gelatin hydrogels (Gel-MA) incorporated with graphene oxide (GO) and metformin-loaded mesoporous silicone nanospheres are synthesized and photo cross-linked to construct a nanocomposite hydrogel (MGO@GM). First, GO nanosheets released from MGO@GM inhibit bacterial biofilm formation and disrupt mature biofilms under near-infrared irradiation. Meanwhile, GO activates the NLRP3 to induce a macrophage-associated proinflammatory response against biofilms. Afterward, with the subsequent degradation of MGO@GM, released metformin reduces local hyperglycemia, downregulates NLRP3, and inhibits NETs formation. Furthermore, repolarized M2 macrophages alleviate the inflammatory microenvironment and promote tissue regeneration. Briefly, this SOIT strategy regulates the NLRP3 and rescues impaired innate immunity to facilitate anti-infection and tissue repair, which provides a new perspective for the future clinical treatment of BIDWs. 相似文献
10.
Siva Nemala Sankar João Fernandes Marília Barreiros dos Santos Begoña Espiña Pedro Alpuim Ander García Díez Senentxu Lanceros-Mendez Lalita Saini Suvigya Kaushik Gopinadhan Kalon Andrea Capasso 《Advanced functional materials》2023,33(23):2214889
Carbon-based materials, such as graphene oxide and reduced graphene oxide membranes have been recently used to fabricate ultrathin, high-flux, and energy-efficient membranes for ionic and molecular sieving in aqueous solution. However, these membranes appeared rather unstable during long-term operation in water with a tendency to swell over time. Membranes produced from pristine, stable, layered graphene materials may overcome these limitations while providing high-level performance. In this paper, an efficient and “green” strategy is proposed to fabricate µm-thick, graphene-based laminates by liquid phase exfoliation in Cyrene and vacuum filtration on a PVDF support. The membranes appear structurally robust and mechanically stable, even after 90 days of operation in water. In ion transport studies, the membranes show size selection (>3.3 Å) and anion-selectivity via the positively charged nanochannels forming the graphene laminate. In antibiotic (tetracycline) diffusion studies under dynamic conditions, the membrane achieve rejection rates higher than 95%. Sizable antibacterial properties are demonstrated in contact method tests with Staphylococcus aureus and Escherichia coli bacteria. Overall, these “green” graphene-based membranes represent a viable option for future water management applications. 相似文献
11.
Implant Materials: Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials (Adv. Funct. Mater. 40/2017) 
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下载免费PDF全文 Brigitte Altmann Lamprini Karygianni Ali Al‐Ahmad Frank Butz Maria Bächle Erik Adolfsson Tobias Fürderer Nicolas Courtois Paola Palmero Marie Follo Jérôme Chevalier Thorsten Steinberg Ralf Joachim Kohal 《Advanced functional materials》2017,27(40)
12.
Brigitte Altmann Lamprini Karygianni Ali Al‐Ahmad Frank Butz Maria Bächle Erik Adolfsson Tobias Fürderer Nicolas Courtois Paola Palmero Marie Follo Jérôme Chevalier Thorsten Steinberg Ralf Joachim Kohal 《Advanced functional materials》2017,27(40)
The development of long‐lasting zirconia‐based ceramics for implants, which are not prone to hydrothermal aging, is not satisfactorily solved. Therefore, this study is conceived as an overall evaluation screening of novel ceria‐stabilized zirconia–alumina–aluminate composite ceramics (ZA8Sr8‐Ce11) with different surface topographies for use in clinical applications. Ceria‐stabilized zirconia is chosen as the matrix for the composite material, due to its lower susceptibility to aging than yttria‐stabilized zirconia (3Y‐TZP). This assessment is carried out on three preclinical investigation levels, indicating an overall biocompatibility of ceria‐stabilized zirconia‐based ceramics, both in vitro and in vivo. Long‐term attachment and mineralized extracellular matrix (ECM) deposition of primary osteoblasts are the most distinct on porous ZA8Sr8‐Ce11p surfaces, while ECM attachment on 3Y‐TZP and ZA8Sr8‐Ce11 with compact surface texture is poor. In this regard, the animal study confirms the porous ZA8Sr8‐Ce11p to be the most favorable material, showing the highest bone‐to‐implant contact values and implant stability post implantation in comparison with control groups. Moreover, the microbiological evaluation reveals no favoritism of biofilm formation on the porous ZA8Sr8‐Ce11p when compared to a smooth control surface. Hence, together with the in vitro in vivo assessment analogy, the promising clinical potential of this novel ZA8Sr8‐Ce11 as an implant material is demonstrated. 相似文献
13.
Jing Lin Xianfang Cai Zili Liu Nan Liu Min Xie BingPu Zhou Huaquan Wang Zhanhu Guo 《Advanced functional materials》2020,30(23)
As a large number of strain sensors are put into practical use, their stability should be considered, especially in harsh environments containing water or microorganisms, which could affect strain sensing. Herein, a novel strategy to overcome liquid interference is proposed. The strain sensor is constructed with a sandwich architecture through layer‐by‐layer (LBL) spray‐coating of a 3‐(aminopropyl)triethoxysilane (APTES) bonding layer and multi‐walled carbon nanotubes/graphene (MWCNT/G) conductive layers on an elastomeric polydimethysiloxane (PDMS) substrate, and is further decorated with silver (Ag) nanoparticles and the (heptadecafluoro‐1,1,2,2‐tetradecyl) trimethoxysilane (FAS, F in short) to obtain a F/Ag/MWCNG/G‐PDMS (FAMG) strain sensor. The superhydrophobicity and underwater oleophobicity of the outer cover layer causes this FAMG strain sensor surface to exhibit stable strain sensing resistant to liquid interference upon stretching in the Cassie?Baxter wetting state, and resistance to bacterial adhesion (Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)). The sensor attains ultrasensitivity (with a maximum gauge factor of 1989 in the condition of liquid interference), broad strain range (0.1–170%), fast response time (150 ms), and stable response after 1000 stretching–releasing cycles. The ultrasensitivity is provided by propagation of cracks in MWCNT/G conductive layers and terminal fracture of the intermediate separating layers (APTES/MWCNT/G). The microbridge effect of MWCNTs and slippage of APTES/MWCNT/G provide a large strain range. The FAMG strain sensor is successfully used to monitor a series of human activities and an electronic bird under artificial rain and bacterial droplets, indicating the potential use of this sensor in complex environments. 相似文献
14.
Pasquale Sacco Gabriele Baj Fioretta Asaro Eleonora Marsich Ivan Donati 《Advanced functional materials》2020,30(31)
Recent evidence has led to the hypothesis that dissipation of energy through the viscoelastic extracellular matrix (ECM) can play a cardinal role in directing cell‐fate decisions, but whether and how it correlates with specific cell response is at present unclear. Here, viscoelastic and plastic 2D chitosan‐based substrates endowed with different dissipative energies are developed and cell behavior studied in terms of adhesion and spreading. While keeping constant stress relaxation and systematically decoupling overall stiffness from linear elongation, an energy dissipation term (J mol?1) is introduced, that is the molar energy required to deviate from linear stress–strain regime and enter into plastic region. Strikingly, an inverse relationship is unveiled between substrate dissipation energy and cell response, with high adhesion/high spreading and low adhesion/no spreading detected for substrates at low and high dissipation energy, respectively. It is concluded that cells decide how to react depending on the effective energy they can earmark for their functions. 相似文献
15.
Cairong Li Wei Zhang Yangyi Nie Dongchun Jiang Jingyi Jia Wenjing Zhang Long Li Zhenyu Yao Ling Qin Yuxiao Lai 《Advanced functional materials》2023,33(20):2214158
Bioinspired scaffolds with two distinct regions resembling stratified anatomical architecture provide potential strategies for osteochondral defect repair and are studied in preclinical animals. However, delamination of the two layers often causes tissue disjunction between the regenerated cartilage and subchondral bone, leading to few commercially available clinical applications. This study develops an integrated poly(ε-caprolactone) (PCL)-based scaffold for repairing osteochondral defects. An extracellular matrix (ECM)-incorporated 3D printing composite scaffold (ECM/PCL) coated with ECM hydrogel (E-co-E/PCL) is fabricated as the upper layer, and magnesium oxide nanoparticles coated with polydopamine (MgO@PDA)-incorporated composite scaffold (MD/PCL) is fabricated using 3D printing as the bottom layer. The physicochemical and mechanical properties of the bilayer scaffold meet the requirements in designing and fabricating the osteochondral scaffold, especially a strong interface possessed between the two layers. By in vitro study, the integrated scaffold stimulates proliferation, chondrogenic differentiation, and osteogenic differentiation of human bone mesenchymal stem cells. Moreover, the integrated bilayer scaffold exhibits well repair ability to facilitate simultaneous regeneration of cartilage and subchondral bone after implanting into the osteochondral defect in rats. In addition, cartilage “tidemarks” completely regenerated after 12 weeks of implantation of the bilayer scaffold, which indicates no tissue disjunctions formed between the regenerated cartilage and subchondral bone. 相似文献
16.
聚苯醚基材的对称、低活性分子结构使其具有十分稳定的化学性质,因此难以直接利用化学镀技术在基材表面沉积出高质量的金属层。针对此问题,文中采用化学蚀刻与多巴胺接枝相结合的方法对PPO基材进行表面改性处理,以调整表面粗糙度,改善表面亲水性。文中引入对Ag+具有吸附作用的活性官能团,提高了沉积金属层的导电性和附着力。各项表征测试证明了经过该方法制备出的铜层呈现出连续、均匀且致密堆积的形貌,并具有高纯度、良好的结晶度、优异的附着力以及低电阻率。 相似文献
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18.
Famin Qiu Satoshi Fujita Rami Mhanna Li Zhang Benjamin R. Simona Bradley J. Nelson 《Advanced functional materials》2015,25(11):1666-1671
Artificial micro‐/nanoswimmers have various potential applications including minimally invasive diagnosis and targeted therapies, environmental sensing and monitoring, cell manipulation and analysis, and lab‐on‐a‐chip devices. Inspired by natural motile bacteria such as E. Coli, artificial bacterial flagella (ABFs) are one kind of magnetic helical microswimmers. ABFs can perform 3D navigation in a controllable fashion with micrometer precision under low‐strength rotating magnetic fields (<10 mT) and are promising tools for targeted drug delivery in vitro and in vivo. In this work, the successful wirelessly targeted and single‐cell gene delivery to human embryonic kidney (HEK 293) cells using ABFs loaded with plasmid DNA (pDNA) in vitro is demonstrated for the first time. The ABFs are functionalized with lipoplexes containing pDNA to generate functionalized ABFs (f‐ABFs). The f‐ABFs are steered wirelessly by low‐strength rotating magnetic fields and deliver the loaded pDNA into targeted cells. The cells targeted by f‐ABFs are successfully transfected by the transported pDNA and expressed the encoding protein. These f‐ABFs may also be useful for in vivo gene delivery and other applications such as sensors, actuators, cell biology, and lab‐on‐a‐chip environments. 相似文献
19.
Zhenhong Luo Hengqing Cui Jiangna Guo Jieran Yao Xia Fang Feng Yan Bin Wang Hailei Mao 《Advanced functional materials》2021,31(23):2100336
Resistant bacteria have become a global threat. Even if bacteria are killed, their carried drug-resistant genes can remain in the environment and spread to other microbes via horizontal gene transfer. Development of antimicrobial materials with intrinsic gene break down activity can prevent the dissemination of released drug-resistant genes from the dead bacteria. Herein, imidazolium type poly(ionic liquid) (PIL)/cerium (IV) ion-based electrospun nanofibrous membranes (PIL-Ce) are synthesized. The effects of PIL and Ce moieties on the antimicrobial properties against Gram-negative Escherichia coli and kanamycin-resistant E. coli, and Gram-positive Staphylococcus aureus and methicillin-resistant S. aureus (MRSA), as well as deoxyribonuclease-mimic activities to the drug-resistant genes of KanR (E. coli) and mecA (MRSA) are investigated. The Ce-containing PIL membranes show the high efficiencies to eradicate bacteria and disintegrate drug-resistant genes. A wound treatment test using MRSA infected mice as the model further demonstrate that PIL-Ce membranes combine both antibacterial and DNase-mimic properties, and may have the potential application as a new “green” wound dressing to block the drug resistance spread in a clinical setting. 相似文献
20.
Chohei Pang Jae-Kang Kim Yingdan Wu Michael Yu Wang Hongyu Yu Metin Sitti 《Advanced functional materials》2023,33(38):2303116
The rapid growth in the miniaturized mechanical and electronic devices industry has created the need for temporary attachment systems that can carry out pick-and-place and transfer printing tasks for fragile and tiny parts. Current systems are limited by a fundamental trade-off between adhesive strength and state-changing trigger force, which causes the need for a rapidly switchable adhesive. In this study, an elastomeric microstructure is presented combining a trapezoidal-prism-shaped (TPS) and a mushroom-shaped microstructure, which overcomes the trade-off with the help of the TPS structure. The optimal design exhibits a strong adhesive strength of 87.8 kPa and a negligible detachment strength of <0.07 kPa with a low trigger shear stress of 10.7 kPa on smooth glass surfaces. The large tip-to-stem ratio (50 to 20 µm) enhances the suction effect, allowing the microstructure to maintain its adhesive performance even in wet conditions. Pick-and-place manipulation tasks of a single and an array of ultralight parts from micrometer to millimeter scales are performed to demonstrate the capability of handling fragile and tiny parts. Moreover, it demonstrates the ability to transfer parts across water and air interfaces. This proposed microstructure offers a facile solution for manipulating microscale fragile parts in dry and wet conditions. 相似文献