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The metal binding ability of Maillard reaction products (MRP) was investigated by spectrophotometric monitoring for complex/chelate
formation. In addition, the antioxidant activity of MRP, in the presence of Cu(II), was evaluated on a lipid model system
consisting of sunflower seed oil/water (1∶2). Results from the Rancimat test indicated a significant decrease in pro-oxidant
ability of Cu(II) in the presence of MRP in contrast to histidine alone, whether heated or not. Results from the diphenylpicrylhydrazyl
method showed that, in contrast to histidine (heated or not), the antiradical ability of MRP is reduced in the presence of
Cu(II), but depends on the reaction time between MRP and Cu(II). 相似文献
3.
The Antioxidative Effect of Maillard Reaction Products in Model Systems and Roasted Hazelnuts Solutions of glucose and various amino acids were heated in phosphate buffer pH 7.0. Browning and reducing power of these Maillard reaction solutions showed correlation and increase in the ordeaicid -neutral — basic amino acids. Upon addition to emulsions of methyl imoleate (ML) in water the antioxidative effect increased in the order basic — acid — neutral amino acids. The respective amino acids alone had no effect, various Amadori compounds (fructose-alanine. -arginine and -histidine) a prooxidative effect. Extracts from roasted, defatted hazelnuts also showed correlation between browning and reducing power. Water extracts possessed higher browning and reducing power than the corresponding methanol extracts; the antioxidative effect of both extracts in ML emulsions however was comparable and increased with the roasting time as well as with the amount of extract added. The extracts were separated by gel permeation chromatography on Sephadex G-15 into three main fractions. An antioxidative effect was mainly found in the fraction of low molecular colourless Maillard reaction products. 相似文献
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Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin-free egg yolk 总被引:12,自引:0,他引:12
Pyo-Jam Park Won-Kyo Jung Kyung-Soo Nam F. Shahidi Se-Kwon Kim 《Journal of the American Oil Chemists' Society》2001,78(6):651-656
The protein extracted from lecithin-free egg yolk, normally discarded by lecithin processing plants, was hydrolyzed with the
aid of Alcalase, a commercial enzyme. The hydrolysate was separated through a series of ultrafiltration membranes with molecular
weight cutoffs of 10, 5, and 1 kDa; and three types of permeates including 10 K (permeate from 10 kDa), 5 K (permeate from
5 kDa), and 1 K (permeate from 1 kDa) were obtained. The antioxidative efficacy of hydrolysates so obtained was investigated
and compared with α-tocopherol. Furthermore, two different peptides showing strong antioxidative activity were isolated from
the hydrolysates by using consecutive chromatographic methods including ion exchange chromatography on a SP-Sephadex C-25
column, gel filtration on a Sephadex G-25 column, and high-performance liquid chromatography on an octadecylsilane column.
The purity of the peptides was identified using capillary electrophoresis. The isolated peptides were composed of 10 and 15
amino acid residues, and both contained a leucine residue at their N-terminal positions. 相似文献
6.
Xubo Fang Ningning Xie Xiaoe Chen Hui Yu Jing Chen 《Food and Bioproducts Processing》2012,90(4):676-682
The squid muscle protein, extracted from by-products of flying squid (Ommastrephes bartrami) was hydrolyzed by five proteases (pepsin, trypsin, papain, alcalase and flavourzyme). DPPH radical scavenging power was used to evaluate antioxidative activity of hydrolysates. The hydrolysate obtained by papain exhibited the most excellent potential of antioxidative activity. Furthermore, response surface methodology (RSM) was employed to optimize hydrolysis conditions, including enzyme to substrate (E/S) ratio, reaction temperature, and hydrolysis time. The optimum conditions obtained were as follows: E/S ratio of 1.74%, temperature of 51 °C and time of 46 min, under which, DPPH radical scavenging activity of 74.25% was obtained. Moreover, it was found that the optimum hydrolysate of 8 mg/mL displayed relatively stronger inhibitory effect on lipid peroxidation compared with α-Tocopherol of 0.1 mg/mL. 相似文献
7.
Roasting-Related Changes in Oxidative Stability and Antioxidant Capacity of Apricot Kernel Oil 总被引:1,自引:0,他引:1
Gökhan Durmaz İhsan Karabulut Ali Topçu Meltem Asiltürk Türkan Kutlu 《Journal of the American Oil Chemists' Society》2010,87(4):401-409
Apricot kernels were roasted for various lengths of time (0–30 min) at 180 °C and changes in the oxidative stability, antioxidant
capacity, color, as well as the level of tocopherols and fatty acids of the apricot kernel oil (AKO) were monitored. While
the level of tocopherols decreased, the oxidative stability and antioxidant capacity of AKO increased with roasting, probably
due to the formation of antioxidative Maillard reaction products (MRPs) during the roasting. Medium roasted samples (15–20 min)
were found to be more resistant to oxidative deterioration. The oil from the 30-min roasted sample was more susceptible to
oxidation compared to the oil from the 20-min roasted sample in most of the stability tests. Relatively shorter roasting periods
(5–10 min) also led to a decrease in oxidative stability in comparison to the unroasted sample. Brownish color and antiradical
activity increased with roasting and the highest values were measured in the 30 min roasted sample. 相似文献
8.
The functional characteristics which include protein solubility at different pH, emulsifying and foaming properties, degree of hydrolysis, molecular weight distribution, antioxidant and ACE inhibitory activity of sesame protein hydrolysates prepared with pepsin, papain and alcalase enzymes were evaluated. The rate of degree of hydrolysis was found to reach maximum (25–30%) within the first time fragment i.e 10 min but 80% of hydrolysis was obtained in 120 min with alcalase. SDS-PAGE of hydrolysates with papain, pepsin and alcalase evinced bands of low molecular weight protein of 14.3 kDa and even lower for alcalase treatment of 120 min. Hydrolysates so formed were of improved functional properties as evident from emulsifying and foaming property. Hydrolysis with different proteases enhanced the protein solubility significantly at pH 7.0. Antioxidative assay revealed radical scavenging activity of the hydrolysates with papain hydrolysates showing maximum antioxidative efficacy. The ultra-filtered peptide fractions which showed comparable ACE inhibitory activity were sequenced by MALDI-TOF and matched to that of previously identified ACE inhibitory peptides. The results corroborate the ACE inhibitory effect of the peptides. Hence, these highly bioactive protein hydrolysates produced from waste sesame meals can be successfully employed in various functional food formulations. 相似文献
9.
B. E. Elizalde F. Bressa M. Dalla Rosa 《Journal of the American Oil Chemists' Society》1992,69(4):331-334
Maillard reaction volatile compounds were prepared by heating a glucose-glycine solution. The antioxidant effect of the volatiles
was tested in soybean oil (SBO) thermoxidation. Volatile compounds were transferred from the heated Maillard solution to the
oil with a gas-tight syringe after removing the same volume of oil headspace (air). Standard accelerated oxidation was performed
by heating the SBO at 90°C. Antioxidant activity was evaluated through peroxide value and headspace gas chromatographic analysis
of oil volatile compounds. Furthermore, some indices, such as protection factor and oxidation kinetic rate, were used to measure
the antioxidant effect. Maillard volatile effectiveness was related to browning level of glucose-glycine solution. The maximum
antioxidant effect was obtained with volatiles from 12–18 hr of heating the glucose-glycine solution. This result is related
to the quantity of Maillard volatiles transferred into the oil atmosphere and to the reduction power of the browned Maillard
solution.
Research fellow of Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina. 相似文献
10.
Xuebin Lu Yimin Zhang Ying Liang Jing Yang Shuting Zhang Eiji Suzuki 《Korean Journal of Chemical Engineering》2008,25(2):302-307
The object of this work was to study the xylose production by hydrolysis of corn stover with diluted sulfuric acid at 100
°C. Several concentrations of H2SO4 (2%, 4% and 6% w/w) and reaction time (0–300 min) were evaluated. Kinetic parameters of mathematical models for predicting
the concentrations of xylose, glucose and furfural in the hydrolysates were found. Optimal conditions for hydrolysis were
5.5% H2SO4 at 100 °C for 60 min; under these conditions, 86.7% of xylose yield and 2.82 g/g selectivity were attained, leading to liquors
containing up to 18.73 g/l xylose, 6.64 g/l glucose and 0.63 g/l furfural. The models could be successfully used to predict the concentrations of xylose, glucose and furfural within 0–300
min under experimental acid concentrations. Furthermore, the hydrolysis process of corn stover using dilute acid could be
conceived as the first stage of an integrated strategy for corn stover utilization. 相似文献
11.
Lin Li Lin Wang Jiang Liu Jiawei Yang Wenxia Wu Bo Zhang Wenyu Liu Changqing Wei 《Journal of the American Oil Chemists' Society》2020,97(12):1385-1399
Defatted flaxseed meal, as a high-protein by-product can be used as a good raw material for the Maillard reaction (MR). A MR model system was established using glucose, fructose, and glucose/fructose (1:1) to produce volatile compounds typically obtained from defatted flaxseed meal protein hydrolysate; these compounds were analyzed using head-space solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC–MS). From the 12 MR model reaction groups, the production of pyrazines was between 12.76% and 26.56%. In the 1G group (the group prepared using DH1 = 7.87% with a glucose concentration of 10%), the amount of pyrazines generated reached 26.56%, and this was higher than that of the other 11 model reaction groups. The effect of reaction temperature (100–150 °C), reaction time (10–60 min), and sugar concentration (5–30%) on pyrazine content was determined using the 1G group. The results of this study indicated that the effect of temperature on pyrazine production was higher than that exerted by reaction time and glucose addition. The pyrazine content reached the maximum at a reaction temperature of 140 °C. The degree of reaction and pH value were also determined; the results demonstrated that accumulation of pyrazines was suboptimal when the pH of the system was dramatically decreased. For the overall reaction, the pyrazine content remained similar to that of the final products; however, the pyrazine content exhibited little correlation with the intermediate products in the MR. 相似文献
12.
Ljiljana Popović Draginja Peričin Žužana Vaštag Senka Popović Vera Krimer Aleksandra Torbica 《Journal of the American Oil Chemists' Society》2013,90(8):1157-1165
Cucurbitin extracted from pumpkin (Cucurbita pepo) oil cake was enzymatically hydrolysed with three different enzymes viz. alcalase, flavourzyme and pepsin. Antioxidative and functional properties of cucurbitin hydrolysates with different degrees of hydrolysis (DH) were investigated. The antioxidant activity of the hydrolysates was strongly dependent on the enzyme used. The hydrolysates obtained by alcalase and pepsin showed antioxidative potential whereas flavourzyme hydrolysates did not demonstrate these activities. Reducing power and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging activity of cucurbitin hydrolysate were positively related to DH. The highest antioxidant activity was found in the hydrolysate obtained by alcalase at DH 25.6 % [reducing power of 0.25 ± 0.01 A700 nm and ABTS scavenging activity of 3.34 ± 0.02 mmol/L Trolox equivalent antioxidant coefficients (TEAC)]. Hydrolysis by all enzymes increased the protein solubility within the studied pH range. The best emulsion activity and stability index (EAI = 143.28 ± 3.05 m2/g and ESI = 87.5 ± 1.96 min) have hydrolysates produced by flavourzyme (DH 9.2 %) whereas alcalase produced hydrolysates with the best foaming capacity (FC = 242 ± 3.21). The results demonstrate that hydrolysates produced in the present study have good functional properties as well as antioxidant activity indicating their possible use in different food systems. 相似文献
13.
Casimir C. Akoh 《Journal of the American Oil Chemists' Society》1994,71(3):319-323
Two immobilized lipases fromCandida antarctica (SP 382) andC. cylindraceae, nowrugosa (2001), catalyzed the synthesis of novel acetylated glucose fatty acid esters with glucose pentaacetate (GP) and Trisun 80
(80% oleic) vegetable oil or methyl oleate as substrates in organic solvents. The relative yield was between 6.4–52%, and
the incorporation of oleic acid onto the glucose was between 31–100%. In addition, these enzymes were able to catalyze the
synthesis of glucose fatty acid esters with free glucose as the sugar substrate. The highest oleic acid incorporation (100%)
was obtained in benzene with SP 382 lipase and Trisun 80 as the acyl donor. With methyl oleate as the acyl donor, greater
incorporation was obtained in benzene (90.5%) compared to 75% in isooctane. The 2001 lipase was better in benzene/pyridine
(2∶1 vol/vol) 74%) and chloroform (61%) compared to benzene and isooctane. However, with free glucose and Trisun 80 as substrates,
both enzymes gave acceptable levels of oleic acid incorporation (82–100%) in benzene, benzene/pyridine and pyridine. The best
conditions for the ester interchange reaction reported are: lipase (10% by weight of substrate); incubation time 48 h; molar
ratio of Trisun/GP 1∶2; 3 mL solvent and 3% added water. These glucose esters have potential applications as emulsifiers in
food, cosmetics and pharmaceutical formulations. 相似文献
14.
Jinchao WEI Qipeng YUAN Tianxin WANG Le WANG 《Frontiers of Chemical Science and Engineering》2010,4(1):57
Xylitol, a five-carbon sugar alcohol, is a valuable sugar substitute, and widely used in the pharmaceutical, odontological and food industry due to its interesting properties. In the past decades, the xylitol industry has grown rapidly and more attention has been focused on xylitol purification, which possesses an important proportion of the whole industry. In our paper, the purification and crystallization of xylitol fermentation broth by biotechnology using corncob hydrolysates as substance were studied. An initial xylitol fermentation broth was decolored with activated carbon (1% M-1, 60°C, 165rpm), desalted with a combination of two ion-exchange resins (732 and D301), and residual sugars were separated with UBK-555(Ca2+). Then the solution was vacuum-concentrated up to supersaturation (750g/L xylitol). After adding 1% xylitol crystal seeds, the supersaturated solution was cooled to −20°C for 48h. The crystalline xylitol of a regular tetrahedral shape with purity 95% and crystallization yield 60.2% was obtained from the clarified xylitol fermentation broth. An intact, economical and environmental-friendly route of purification and crystallization of xylitol from fermentation of corncob hydrolysates was obtained, and its experimental procedure and data provided a sound basis for large-scale industrial production. 相似文献
15.
Takanori Tsuda Toshihiko Osawa Tsutomu Nakayama Shunro Kawakishi Katsumi Ohshima 《Journal of the American Oil Chemists' Society》1993,70(9):909-913
Antioxidative activity of pea bean (Phaseolus vulgaris L.) extract was evaluated by using a linoleic acid system, and the methanol extract exhibited strong antioxidative activity
as measured by the thiocyanate method. The crude methanol extract was partitioned between then-butanol phase (BP) and the water phase (WP). Then, the antioxidative activity of the BP and the WP was determined by using
a linoleic acid system. The WP showed strong antioxidative activity, while BP showed only weak activity as measured by the
thiocyanate method. Next, the synergistic antioxidative action of WP with α-tocopherol was examined by using linoleic acid
and liposome systems. The WP had a synergistic effect with α-tocopherol in both the food model and liposome systems. For purification
and isolation of the antioxidative substances of the pea bean, preparative high-performance liquid chromatography was carried
out with an octadecylsilyl column. Five fractions were collected, and antioxidative activity was determined in a linoleic
acid system. Although fraction 1 had strong activity by the thiocyanate method, the purification of this active fraction was
difficult; therefore, the partly characterized active fraction was investigated. The contents of total phenolics and sugars
were 0.31±0.01 mg/g of fraction 1 and 406.1±0.1 mg/g, respectively. The ninhydrin chromogenic reaction was positive, and the
ultraviolet absorption spectral λ max value in distilled water was 264.0 nm, indicating that the water-soluble antioxidative
components from pea bean may be a new type of antioxidant. Isolation and identification are currently being investigated. 相似文献
16.
Recently, we have reported that anthocyanins show strong antioxidative activity, but no attention has been paid to anthocyanins
from the viewpoint of the reaction mechanism of alkylperoxyl radicals; therefore, we investigated the reaction products of
antioxidative anthocyanins (cyanidin 3-O-β-d-glucoside). Cyanidin 3-O-β-d-glucoside was reacted with 2,2′-azobis(2,4-dimet hylvaleronitrile) to generate the alkylperoxyl radicals, and the reaction
products were isolated by high-performance liquid chromatography. The products were identified as 4,6-dihydroxy-2-O-β-d-glucosyl-3-oxo-2,3-dihydrobenzofuran and protocatechuic acid. Based on reaction products, the antioxidative mechanism of
cyanidin 3-O-β-d-glucoside may be different from that of α-tocopherol; cyanidin 3-O-β-d-glucoside would produce another radical scavenger, as it would break down the structure and scavenge the radicals. 相似文献
17.
Optimization of the Aqueous Enzymatic Extraction of Rapeseed Oil and Protein Hydrolysates 总被引:3,自引:0,他引:3
Shao Bing Zhang Zhang Wang Shi Ying Xu 《Journal of the American Oil Chemists' Society》2007,84(1):97-105
An aqueous enzymatic extraction method was developed to obtain free oil and protein hydrolysates from dehulled rapeseeds.
The rapeseed slurry was treated by the chosen combination of pectinase, cellulase, and β-glucanase (4:1:1, v/v/v) at concentration
of 2.5% (v/w) for 4 h. This was followed by sequential treatments consisting of alkaline extraction and an alkaline protease
(Alcalase 2.4L) hydrolysis to both produce a protein hydrolysate product and demulsify the oil. Response surface methodology
(RSM) was used to study and optimize the effects of the pH of the alkaline extraction (9.0, 10.0 and 11.0), the concentration
of the Alcalase 2.4L (0.5, 1.0 and 1.5%, v/w), and the duration of the hydrolysis (60, 120, and 180 min). Increasing the concentration
of Alcalase 2.4L and the duration of the hydrolysis time significantly increased the yields of free oil and protein hydrolysates
and the degree of protein hydrolysis (DH), while the alkaline extraction pH had a significant effect only on the yield of
the protein hydrolysates. Following an alkaline extraction at pH 10 for 30 min, we defined a practical optimum protocol consisting
of a concentration of 1.25–1.5% Alcalase 2.4L and a hydrolysis time between 150 and 180 min. Under these conditions, the yields
of free oil and protein hydrolysates were 73–76% and 80–83%, respectively. The hydrolysates consisted of approximately 96%
of peptides with a MW less than 1500, of which about 81% had a MW less than 600 Da. 相似文献
18.
Downstream Processes for Aqueous Enzymatic Extraction of Rapeseed Oil and Protein Hydrolysates 总被引:1,自引:2,他引:1
Shao Bing Zhang Zhang Wang Shi Ying Xu 《Journal of the American Oil Chemists' Society》2007,84(7):693-700
Downstream processes following aqueous enzymatic extraction (AEE) of rapeseed oil and protein hydrolysates were developed
to enhance the oil and protein yields as well as to purify the protein hydrolysates. The wet precipitate (meal residue) from
the AEE was washed with twofold water at 60 °C, pH 11 for 1 h. Emulsions from the AEE and the washing step were pooled and
submitted to a stepwise demulsification procedure consisting of storage-centrifugation and freezing–thawing followed by centrifugation.
Aqueous phases were pooled and adsorbed onto macroporous adsorption resins (MAR) to remove salts and sugars. Following extensive
rinsing with deionized water (pH 4), desorption was achieved by washing with 85% ethanol (v/v) to obtain crude rapeseed peptides
(CRPs). In a separate experiment, stepwise desorption was carried out with 25, 55, and 85% ethanol to separate the bitter
peptides from the other peptides. Using a combination of the AEE process, washing and demulsification steps, the yields of
the total free oil and protein hydrolysates were 88–90% and 94–97%, respectively. The protein recovery was 66.7% and the protein
content was enriched from 47.04 to 73.51% in the CRPs. No glucosinolates and phytic acid were detected in the CRPs. From the
stepwise desorption, a non-bitter fraction RP25 (containing 64–66% of total desorbed protein) had a bland color and significantly
higher protein content (81.04%) and hence was the more desirable product. 相似文献
19.
Kwang-Hyoung Lee Kyoung-Soo Ha Sung-Hoon Jo Chong M. Lee Young-Cheul Kim Kwang-Hoe Chung Young-In Kwon 《International journal of molecular sciences》2014,15(5):8352-8359
We have previously reported that Amadori compounds exert anti-diabetic effects by lowering sucrose-induced hyperglycemia in normal Sprague-Dawley rats. In the present study we extended our recent findings to evaluate whether α-glucosidase inhibitor arginyl-fructose (AF) lowers blood glucose level in diabetic db/db mice, a genetic model for type 2 diabetes. The db/db mice were randomly assigned to high-carbohydrate diets (66.1% corn starch) with and without AF (4% in the diet) for 6 weeks. Changes in body weight, blood glucose level, and food intake were measured daily for 42 days. Dietary supplementation of AF resulted in a significant decrease of blood glucose level (p < 0.001) and body weight (p < 0.001). The level of HbA1c, a better indicator of plasma glucose concentration over prolonged periods of time, was also significantly decreased for 6-week period (p < 0.001). Dietary treatment of acarbose® (0.04% in diet), a positive control, also significantly alleviated the level of blood glucose, HbA1c, and body weight. These results indicate that AF Maillard reaction product improves postprandial hyperglycemia by suppressing glucose absorption as well as decreasing HbA1c level. 相似文献
20.
Gelatin hydrolysates with antioxidative activity produced by protease from Bacillus amyloliquefaciens H11 with different hydrolysis times were prepared. Alpha-amino group content and antioxidative activities increased with increasing hydrolysis time (p < 0.05). When gelatin hydrolysate prepared with hydrolysis time of 3 h (GH-3H) was subjected to freeze drying and spray drying, the powder obtained from spray drying showed a decrease in antioxidant activity as measured by DPPH and ABTS radical scavenging activities, ferric-reducing antioxidant power, and metal chelating activity. Spray-dried gelatin hydrolysate (GH-3H-SD) showed higher whiteness with lower fishy odor and off-odor associated with fermentation. This was concomitant with the decreases in several odorous compounds in the sample, except for nonanal, which was higher in comparison with the powder obtained by freeze drying. Thus, spray drying could be an effective drying method to improve color and reduce undesirable odor of gelatin hydrolysate. 相似文献