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Dr. Yansong Wang Bernhard Hoermann Dr. Karolina Pavic Malgorzata Trebacz Dr. Pablo Rios Prof. Dr. Maja Köhn 《Chembiochem : a European journal of chemical biology》2019,20(1):66-71
Protein phosphatase-1 (PP1)-disrupting peptides (PDPs) are selective chemical modulators of PP1 that liberate the active PP1 catalytic subunit from regulatory proteins; thus allowing the dephosphorylation of nearby substrates. We have optimized the original cell-active PDP3 for enhanced stability, and obtained insights into the chemical requirements for stabilizing this 23-mer peptide for cellular applications. The optimized PDP-Nal was used to dissect the involvement of PP1 in the MAPK signaling cascade. Specifically, we have demonstrated that, in human osteosarcoma (U2OS) cells, phosphoMEK1/2 is a direct substrate of PP1, whereas dephosphorylation of phosphoERK1/2 is indirect and likely mediated through enhanced tyrosine phosphatase activity after PDP-mediated PP1 activation. Thus, as liberators of PP1 activity, PDPs represent a valuable tool for identifying the substrates of PP1 and understanding its role in diverse signaling cascades. 相似文献
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Cover Feature: Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding (ChemBioChem 23/2017) 下载免费PDF全文
Florian Lehner Dr. Denis Kudlinzki Dr. Christian Richter Dr. Henrike M. Müller‐Werkmeister Katharina B. Eberl Prof. Dr. Jens Bredenbeck Prof. Dr. Harald Schwalbe Dr. Robert Silvers 《Chembiochem : a European journal of chemical biology》2017,18(23):2294-2294
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Cover Picture: A Helix‐Stabilized Cell‐Penetrating Peptide as an Intracellular Delivery Tool (ChemBioChem 2/2016) 下载免费PDF全文
Hiroko Yamashita Prof. Makoto Oba Dr. Takashi Misawa Prof. Masakazu Tanaka Dr. Takayuki Hattori Dr. Mikihiko Naito Dr. Masaaki Kurihara Dr. Yosuke Demizu 《Chembiochem : a European journal of chemical biology》2016,17(2):113-113
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Cover Feature: Kinetic Analysis of PRMT1 Reveals Multifactorial Processivity and a Sequential Ordered Mechanism (ChemBioChem 1/2018) 下载免费PDF全文
Jennifer I. Brown Dr. Timo Koopmans Jolinde van Strien Dr. Nathaniel I. Martin Dr. Adam Frankel 《Chembiochem : a European journal of chemical biology》2018,19(1):2-2
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Front Cover: A Possible Path to Prebiotic Peptides Involving Silica and Hydroxy Acid‐Mediated Amide Bond Formation (ChemBioChem 18/2018) 下载免费PDF全文
Aaron D. McKee Martin Solano Andrew Saydjari Prof. Dr. Christopher J. Bennett Prof. Dr. Nicholas V. Hud Prof. Dr. Thomas M. Orlando 《Chembiochem : a European journal of chemical biology》2018,19(18):1896-1896
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Cover Picture: Peptidyl Succinimidyl Peptides as Taspase 1 Inhibitors (ChemBioChem 15/2014) 下载免费PDF全文
Johannes van den Boom Marija Mamić Daniele Baccelliere Dr. Susanne Zweerink Dr. Farnusch Kaschani Prof. Dr. Shirley Knauer Prof. Dr. Peter Bayer Prof. Dr. Markus Kaiser 《Chembiochem : a European journal of chemical biology》2014,15(15):2165-2165
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Prof. Dr. Emily R. Derbyshire Dr. Vanessa Zuzarte‐Luís Dr. Andreia D. Magalhães Dr. Nobutaka Kato Dr. Paul C. Sanschagrin Dr. Jinhua Wang Dr. Wenjun Zhou Dr. Chandrasekhar V. Miduturu Prof. Dr. Ralph Mazitschek Prof. Dr. Piotr Sliz Prof. Dr. Maria M. Mota Prof. Dr. Nathanael S. Gray Prof. Dr. Jon Clardy 《Chembiochem : a European journal of chemical biology》2014,15(13):1841-1841
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