Studies on dissolved oxygen concentration and sludge retention time affecting the full-scale activated sludge process

We have made a series of experiments on dissolved oxygen (DO) concentration and sludge retention time (SRT) to affect the full-scale activated sludge process.

For DO control, a better effluent water quality and a reduced current consumption have been achieved as compared with those in a constant air flow operation. The best control point for the DO control operation is at the outlet side of the aeration tank.

For SRT control, effluent COD seems to fluctuate slightly, and is favorably stable when SRT is between 10 and 20 days. It takes about twice as many days as SRT value set up for the total mass of sludge in the process (M) to be stabilized in case of making set value change as a result of simulation using mathematical models.     

Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge

The effect of dissolved oxygen (DO) on the communities of ammonia-oxidizing bacteria (AOB) in activated sludge was evaluated in lab-scale and full-scale reactors using the amoA gene as the basis for phylogenetic comparisons. Under controlled laboratory conditions, two chemostats seeded with activated sludge from the same source were operated with high-DO (8.5 mg/L) and low-DO (0.24 and 0.12 mg/L) concentrations for a period of 300 days. At the end of the operation period, the chemostats had enriched AOB communities that belonged to the Nitrosomonas europaea lineage, but were differentiable based on phylogenetic and kinetic analyses. The low-DO chemostat harbored the growth of two different groups within this lineage, differentiable by the amoA sequence comparison and by terminal fragment signatures. The difference in oxygen affinity between high-DO and low-DO enrichments was demonstrated by evaluating the growth kinetics as a function of oxygen concentration. The low-DO enrichment had a higher growth rate at DO concentrations below 4.7 mg/L, but the growth rate significantly decreased at higher DO concentrations, for which the high-DO enrichment experienced higher growth rates. In addition, the dynamic changes in AOB populations in two parallel trains within one full-scale treatment plant were evaluated in response to a significant reduction of DO in one of the treatment trains. Only the train operated with DO concentrations below 1mg/L favored the establishment of a population of AOB related to the N. europaea lineage.     

Response of dissolved oxygen to changes in influent organic loading to activated sludge systems

The major objective of this investigation was to examine the response of the activated sludge process to transient organic loadings and to evaluate the applicability of dissolved oxygen (DO) concentration and oxygen uptake rate as process control variables.A modified laboratory-scale activated sludge unit was used to continuously measure the ambient dissolved oxygen level. The change of the dissolved oxygen concentration responded to variations in the influent composition of the wastewater. First, the system was maintained at steady-state and system variables were measured for soluble TOC, MLVSS and oxygen uptake rate. The organic shock loading was induced by increasing or decreasing the baseline feeding of TOC concentration. As soon as a transient loading occurred the DO levels in the system were continuously monitored with the DO analyzer.A simplified material balance equation for dissolved oxygen in the system was developed and the dynamic behavior of oxygen uptake rate was examined. It was demonstrated that under transient conditions the changes in the dissolved oxygen concentration reflected the variations in the exogenous respiration rate of the biomass in the system due to fluctuations in the influent waste characteristics. Based upon the rapid response to the shock loading and the correlation of the change in the DO level to the magnitude of the shock loading, the two variables (DO concentration and oxygen uptake rate) generated from this biological monitor showed potential as a control variable for the activated sludge process.     

Nitrification process in activated sludge with suspended marble particles

The process of nitrification in activated sludge was investigated. As the solid support for nitrifiers' growth a suspension of marble particles has been used. The results proved the possibility of successful nitrification of 100 mg l^?1 NH₄-N simultaneously with the removal of 600 mg l^?1 COD.     

Microbial viability measurements and activated sludge kinetics

A laboratory study was performed to evaluate the influence of microbial viability information on a widely used steady-state kinetic model of slurry-type biological wastewater treatment processes. Adenosine triphosphate (ATP), determined by the luciferin-luciferase firefly lantern extract method using a liquid scintillation spectrometer to measure light emission, was used as the indicator of microbial viability. It was found that the microbial viability of activated sludge mixed liquor volatile suspended solids (MLVSS) exhibits a functional relationship with biological solids retention time (θ_c), i.e., being essentially 100% at low values of 0_c and decreasing to a nearly constant value at the higher values of 0_c, typical of activated sludge process operation. Values of the model coefficients were also affected by the microbial viability of the MLVSS.Based on mass balance considerations and ATP measurements, it is proposed that the MLVSS of an activated sludge system treating a soluble organic waste can be divided into the following three fractions: (I) active or viable microbial solids, (2) inert microbial debris solids, and (3) nonviable biodegradable microbial solids. Implications of viability information in design and operation are discussed.     

The dissolved oxygen profile—A valuable tool for control of the activated sludge process

Control of activated sludge process based on measurement of the dissolved oxygen profile has the distinct advantage of using sensors of proven reliability. These measurements, when coupled with the power of an on-line computer, permit the calculation of important variables which cannot be measured directly. The features of the profile which are of major significance are the position of the maximum slope, the value of the maximum slope, the DO concentration at the reactor outlet, the slope of the profile close to the outlet, and the second derivative of the profile towards the outlet. This paper demonstrates that the above mentioned features reflect both the hydraulic and organic loading, reaction rates, and degree of completion of the reactions in the activated sludge process. Simulations are presented to show how these features may be controlled by manipulating the mass of sludge in the reactor and the overall oxygen transfer rate coefficient.     

Nitrification in saline wastewater with high ammonia concentration in an activated sludge unit

A nitrifying activated sludge reactor fed with a high salinity medium was operated efficiently at ammonia loading rates between 1 and 4 g NH4+ -N l(-1) d(-1). The system became completely inefficient at inlet salt concentrations higher than 525 mM due to the mixed inhibition effect of salts and ammonia. The final product was mainly nitrate although dissolved oxygen limitations caused sporadic ammonia and nitrite accumulations. Specific nitrifying activity decreased due to the saline effect. A set of activity tests showed that in the continuous reactor non-adapted biomass is rather more sensitive than biomass to the saline effect. Physical properties of biomass in the reactor (sludge volumetric index and zone settling velocity) were not affected by the saline concentration, a biomass concentration of 20 gVSS l(-1) was achieved.     

The kinetics of protein removal by activated sludge

The removal of bovine serum albumin (BSA) by BSA acclimated activated sludge took place in two stages. Removal during stage 1 was attributed to adsorption. The removal of BSA during stage 2 was slower than stage 1. There was never an accumulation of protein degradation products and removal was accompanied by an increase in the metabolic activity of the activated sludge. The removal of BSA per unit biomass during stage 2 was influenced by both the concentration of BSA and the concentration of activated sludge At high concentrations of BSA, removal was preceded by a lag phase. The rate of removal during stage 2 was constant. Within limits the rate of removal of BSA was related to the substrate to biomass ( ) ratio of the system and to the concentration of BSA expressed per unit biomass remaining in equilibrium following stage 1 of BSA removal.     

Microbial uptake of cadmium and its effects on the biochemical oxygen demand at various temperatures

Uptake of cadmium by microbes at different temperatures has been studied at pH 7. Glycine was used as a source of carbon for microorganisms in the BOD bottle at 20, 30, 40 and 50°C with varying concentrations of cadmium: control 0.0437, 0.437, 0.875 and 1.31 mg 1⁻¹ in each set. The influence of temperatures on the toxic effects of cadmium has been studied with respect to rate constant (k) and ultimate biochemical oxygen demand which were calculated from BOD data using Thomas Graphical method. Consumed cadmium mg 1⁻¹ was determined after eight days and it varied from 14.04 to 32.40% at four temperatures. Highest consumption of Cd was noted in the set at 30 and 40°C and lowest at 50°C.     

Stimulating hydrogenotrophic denitrification in simulated groundwater containing high dissolved oxygen and nitrate concentrations

In agricultural areas, nitrate (NO3-) is a common groundwater pollutant as a result of extensive fertilizer application. At elevated concentrations, NO3- consumption causes methemoglobinemia in infants and has been linked to several cancers; therefore, its removal from groundwater is important. The addition of hydrogen gas (H2) via gas-permeable membranes has been shown to stimulate denitrification in a laboratory-scale reactor. This research, using large columns packed with aquifer material to which a simulated groundwater was fed, was conducted to further identify the conditions required for the use of membrane-delivered H2 in situ. In this study, we show that this novel technology was capable of treating highly contaminated (25 mg/L NO3- -N) and oxygenated (5.5mg/L dissolved oxygen) water, but that nutrient addition and gas pressure adjustment was required. Complete NO3- reduction was possible without the accumulation of either NO2- or N2O when the H2 lumen pressure was increased to 17 psi and phosphate was added to the groundwater. The total organic carbon content of the effluent, 110 cm downgradient of H2 addition, did not increase. The results from these experiments demonstrate that this technology can be optimized to provide effective NO3- removal in even challenging field applications.     

Effects of temperature and dissolved oxygen on sludge properties and their role in bioflocculation and settling

Effects of temperature (mesophilic (35 °C) vs. thermophilic (55 °C)) and dissolved oxygen (DO) concentration (under thermophilic conditions) on sludge properties and their role in bioflocculation and settling were studied using well-controlled sequencing batch reactors fed with a synthetic wastewater comprised of glucose and inorganic nutrients. Under a similar DO level, thermophilic sludge had a poorer bioflocculating ability and settleability than that of mesophilic sludge. Under a thermophilic condition, an increase in DO level led to a poorer settleability and a slightly improved bioflocculating ability. A poorer settleability was related to a higher level of filaments. Analysis of bound extracellular polymeric substances (EPS) indicates that thermophilic sludge had a higher level of total bound EPS content than that of mesophilic sludge under a similar DO level, and an increase in DO resulted in an increase in total bound EPS content in thermophilic sludge. Surface analysis of sludge by X-ray photoelectron spectroscopy (XPS) suggests that significant differences in the surface concentrations of elements N, C, O were observed between thermophilic and mesophilic sludge, implying significant differences in bound EPS composition. The results of gel permeation chromatography indicate that the weight-averaged molecular weight (M_w) of bound EPS covered a range of 1159 Da to 13220 Da. The distribution of EPS “species” at floc surfaces was shown by transmission electron microscopy (TEM) to be uneven; different kinds of nanoscale materials were distributed in a patchy manner at the floc-water interface. The results suggest that it is the role of specific EPS molecules rather than the quantity of bound EPS that determine the difference in bioflocculation behavior between thermophilic and mesophilic sludge. The strategy of increasing the DO level could not solve the biomass separation problems associated with thermophilic sludge.     

Bacterial growth kinetics in the completely mixed activated sludge treatment of fellmongery effluent

Fellmongery effluent was successfully treated (± 85% OA reduction) in a laboratory scale completely mixed activated sludge (CMAS) reactor with sludge recycle and controlled wasting. Varying the sludge age was found to have a significant effect on effluent quality. A mathematical model useful in the design and operation of effluent treatment plants was used to describe microbial growth in the treatment process. In the development of the model it is shown how a clear distinction must be made between the volatile suspended solids and the active volatile suspended solids for the model to accurately describe microbial growth kinetics.     

Metal loadings and removal at a municipal activated sludge plant

Influent total and dissolved concentrations of cadmium, chromium, copper, nickel and zinc to the Edmonton Gold Bar Wastewater Treatment Plant were measured at hourly intervals for several days. Metal concentrations were generally higher than in other large western Canadian cities and exhibited distinct diurnal variations. With the exceptions of nickel and zinc, the metals existed largely in the particulate phase. Samples collected from various points throughout the plant suggested that removal efficiencies for cadmium, chromium and copper were high and those for nickel and zinc were variable and generally low. Metal concentrations in the final effluent did not generally exceed the maximum levels allowed for Canadian drinking waters.     

Hydrolysis and acidification of waste activated sludge at different pHs

The effect of pH from 4.0 to 11.0 on the hydrolysis and acidification of waste activated sludge (WAS) was investigated. Experimental results showed that at room temperature the sludge hydrolysis was in the following order: alkaline>acidic>(neutral and blank test), and between pH 6.0 and 11.0 the sludge hydrolysis increased with pH. The three main components, soluble protein, carbohydrate and volatile fatty acids (VFAs) in the hydrolytic product were analyzed. It was observed that both the soluble protein and carbohydrate increased with pH in the pH range 7.0-11.0, but also increased to a smaller extent with pH from 7.0 to 4.0. The VFAs concentration was also affected by pH. Under alkaline conditions, the VFAs production was significantly higher than under other conditions. The concentration of VFAs on the 8th day of fermentation at pH 4.0, 7.0 and 10.0 was, respectively, 354.49, 842.00 and 2708.02 mg/L, while VFAs in the blank test was only 633.59 mg/L. The VFAs consisted of acetic, propionic, iso-butyric, n-butyric, iso-valeric and n-valeric acids, but acetic, propionic and iso-valeric were the three main products. Also, the release of soluble phosphorus and ammonia and the production of methane was studied during WAS fermentation at different pHs.     

Promotion and inhibition of oxygen transfer under fine bubble aeration by activated sludge

The promotion and inhibition of inactivated and activated sludge on oxygen mass transfer (OMT) were studied using lab‐scale experiments. The results showed that the α‐values and oxygen transfer efficiency (OTE) decreased with increasing mixed liquor suspended solids (MLSS) concentration (1–10 g/L). Although OMT promotion rate by microbial respiration in activated sludge system increased from 39.8–97.5% for the α‐values and OTE, the two parameters were found to fall sharply when MLSS concentration was over 5 g/L. This indicated that the sludge concentration is a major influence factor on OMT in activated sludge system. Such results provide valuable knowledge for the operating optimization of the aeration system in wastewater treatment process.     

Sludge settling and online NAD(P)H fluorescence profiles in wastewater treatment bioreactors operated at low dissolved oxygen concentrations

Biological nitrogen removal via simultaneous nitrification and denitrification (SND) may be achieved in the single-tank bioreactors operated at low dissolved oxygen concentrations (DO). The continuous-stirred tank reactor (CSTR) configuration and the low DO environments employed are; however, more conducive to growth of filamentous bacteria and, thus, poor sludge settling in clarifiers. In this work, a synthetic wastewater was treated in bench-scale (approximately 6L) bioreactors under either cyclic or constant-rate aeration, at various sludge retention times (SRT) and DO. The objective was to evaluate the effects of these factors on the sludge settling indicated by sludge volume index. The cyclic aeration was carried out by alternating the aeration between a higher rate for 1h and a lower (or zero) rate for 30 min. In different experiments, the DO during the period of higher aeration (HDO) was 0.4, 0.6, 0.8, or 2.0 mg/L and the DO during lower aeration (LDO) was 0.0 or 0.2mg/L. The sludge established under the cyclic aeration was found to settle better than that under constant-rate aeration. Shortening SRT also improved the sludge settling significantly. NAD(P)H fluorescence profiles in these bioreactors were monitored using an online fluorometer. A procedure was developed to quantitatively describe the metabolic state of sludge's heterotrophic population on a 0-1 scale using the fluorescence profile, with "0" corresponding to the fully anoxic-denitrifying state and "1" to the fully aerobic state.     

Reduction in chlorine requirements by control of nitrification in an oxygen activated sludge process

The objective of the study was to determine if chlorine could be saved by controlling nitrification in an oxygen activated sludge process so that the effluent would produce an ammonia concentration in the 2–4 mg l⁻¹ range. The hypothesis was that the ammonia would react with the chlorine to produce a stable combined residual at a much lower chlorine dosage than if the effluent was completely nitrified. In the latter case, breakpoint would have to be achieved with, potentially, a substantial amount of chlorine dissipated and lost in side reactions with residual organic materials.The study utilized two portable treatment units in two truck trailers on the site of the proposed 69th Street City of Houston 100 MGD Sewage Treatment Plant. The results indicated that chlorine requirements could be cut by one half or more if nitrification was controlled. The gross savings of chlorine were estimated to be in the one quarter of a million dollar range. Other findings were that there was no apparent effect of pH on chlorine residual in the range of 6.5–7.5; and that there was no apparent effect of operating the nitrification stage with air or high purity oxygen.     

A model of localised oxygen sinks around bacterial colonies within activated sludge

A new mathematical analysis of diffusional resistances of oxygen in activated sludge is developed. It assumes that the distribution of bacteria within activated sludges flocs are in colonies rather than the usual assumption of a homogeneous distribution of bacteria throughout the sludge. The solutions are for steady-state. The bacterial colony model results in a completely different shape of dissolved oxygen gradient in the floc when compared to the homogeneous bacterial distribution model. The new “bacterial colony” model predicts highly localised oxygen demands around the colony with maximum dissolved oxygen deficits in a 20–40 μm dia floc of 2–3 mg l⁻¹.     

Enumeration of acetate-consuming bacteria by microautoradiography under oxygen and nitrate respiring conditions in activated sludge

Microautoradiography was used to enumerate bacteria able to take up radiolabelled acetate in activated sludge using oxygen or nitrate as electron acceptors. In each of three wastewater treatment plants (WWTP) with nitrification and denitrification (N-removal), the number of bacteria consuming acetate under aerobic and anoxic conditions was identical in contrast to the acetate removal rates. The rates were clearly lower under anoxic conditions suggesting that the specific activity of the cells and not the number of active cells was reduced under anoxic conditions. The fraction of bacteria able to consume acetate varied in three WWTPs between 47% and 93% of the total number of bacteria as determined by DAPI. In a WWTP without N-removal only 20% of the bacteria were able to consume acetate under aerobic conditions and very few of these were able to do it under anoxic conditions. The cell specific acetate removal rates in all WWTPs were found to be 3.0-13.2 x 10(-15) mol cell(-1) h(-1) under aerobic conditions and between 1.9 and 9.1 x 10(-15) mol cell(-1) h(-1) under anoxic conditions.