共查询到18条相似文献,搜索用时 140 毫秒
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为了研究多巴在D(L)-2酒石酸异丁酯1,2二氯乙烷有机相和羟丙基-β-环糊精水相萃取体系中的分配行为;运用双相(O/W)识别手性萃取,考察酒石酸构型和浓度、羟丙基-β-环糊精浓度、水相pH值等因素对萃取性能的影响。羟丙基-β-环糊精对S-多巴对映体的识别能力大于对R-多巴对映体的识别能力,而L-酒石酸异丁酯的识别能力刚好相反;在羟丙基-β-环糊精和L-酒石酸异丁酯萃取体系中,多巴外消旋体一次萃取分离后R和S对映体的分配系数(kR和kS)分别为8.92和5.34,分离因子α达1.67;同时pH值和萃取剂浓度对手性分离能力有显著的影响。双相(O/W)识别手性萃取具有较强的手性分离能力,它对外消旋体化合物的制备性分离有着十分重要的意义。 相似文献
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采用非手性的Cu色谱柱,通过在流动相中加入手性选择剂β-环糊精和在样品中加入β-环糊精的方法实现对盐酸西布曲明对映体的拆分.流动相组成为含β-环糊精的甲醇-水(50∶50,V/V,pH=6).当手性选择剂β-环糊精的浓度为0.8mmol/L时,盐酸西布曲明对映体在进样后的5.7min得到了基线分离. 相似文献
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以组氨酸-β-环糊精(β-CD-E_2)衍生物作为手性选择剂,利用原位聚合反应制得新型β-CD衍生物手性高效毛细管电泳(HPCE)整体柱,将其应用于手性药物羟丙哌嗪的消旋体拆分。分别探究了不同缓冲体系的洗脱能力、缓冲液pH对分离的影响以及羟丙哌嗪手性拆分的线性范围。结果表明,在最佳条件下,羟丙哌嗪对映体在β-CD-E_2整体柱上能得到较好拆分,分离度Rs达到40.52,且羟丙哌嗪浓度在9.8×10~(-6)~2.0×10~(-5)mol/L范围内与对映体峰高、峰面积具有一定的线性关系。此工作将β-CD-E_2作为HPCE固定相,为手性药物羟丙哌嗪建立了一种新的分离分析体系。 相似文献
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HPLC手性流动相添加剂法拆分盐酸美西律对映体 总被引:1,自引:0,他引:1
以羟丙基-β-环糊精(HP-β-CD)作为手性流动相添加剂,于C18柱高效液相色谱建立了盐酸美西律对映体的拆分方法.方法:本文选用ODS Hypersil柱(4.6 mm×200 mm,4.5μm),以羟丙基-β-环糊精(HP-β-CD)作为手性流动相添加剂,流动相:乙腈-水(30:70,v/v),其中含17mmol·L-1HP-β-CD.pH值为6.32,流速0.20mL·min-1;紫外检测波长:261nm;进样量:10μL.考察了流动相种类、pH值和手性流动相添加剂浓度等因素对手性拆分的影响.结果:建立了羟丙基-β-环糊精手性流动相添加剂法拆分盐酸美西律对映体的方法.结论:此方法简便、快速,分离度好. 相似文献
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以羟丙基-β-环糊精(HP-β-CD)为手性流动相添加剂,研究了氟比洛芬对映体在反相高效液相色谱中的拆分。考察了羟丙基-β-环糊精的浓度、流动相pH值、有机调节剂、柱温对手性分离的影响,同时探讨了HP-β-CD对氟比洛芬对映体在反相高效液相中的分离机制且计算出相应的包结常数。确定了色谱条件:YMC-Pack ODS-A C18(150 mm×4.6 mm,5μm)色谱柱,流动相为0.5%乙酸(pH3.5,三乙胺调节)含25 mmol/L羟丙基-β-环糊精:甲醇(80:20,v/v),流速为1.0 mL/min,柱温为30℃,紫外检测波长为247 nm。通过容量因子(k’)的倒数1/k’对[HP-β-CD]的良好的线性关系证明氟比洛芬与HP-β-CD形成包结比为1:1包结物,(+)-氟比洛芬的包结常数为1.83 L/mol,(-)-氟比洛芬的包结常数为1.67 L/mol。 相似文献
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分别将β-环糊精、2,6-二甲基-β-环糊精和2,3,6-三甲基-β-环糊精作为手性流动相添加剂,系统地研究了乳酸对映体在反相HPLC系统中的拆分,考察了流动相种类、pH值、流速和手性流动相添加剂的浓度对手性分离的影响,建立了甲基化β-环糊精动态手性固定相法分离乳酸对映体的方法.工作曲线的线性范围为0.02~0.18g/L(D-,L-乳酸),线性相关系数为rL-乳酸=0.9990,rD-乳酸=0.9890,精密度(n=8)为RSDLL-乳酸=0.80%,RSDD-乳酸=1.54%. 相似文献
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采用β-环糊精超分子体系对映体利用荧光分析法进行手性识别作了研究。阐明了β-环糊精对不同氨基酸对映体的手性包络作用。 相似文献
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《分离科学与技术》2012,47(13):2099-2109
This paper reports on the determination of the intrinsic kinetics in biphasic recognition chiral extraction of phenylsuccinic acid enantiomers (H2A) by L-IBTA and HP-β-CD in a modified Lewis cell. The two-phase homogeneous reaction model was selected, because there is a finite physical solubility of phenylsuccinic acid enantiomers in both the aqueous phase and the organic phase. The regime analysis was split up in three parts: a regime analysis on the aqueous phase reaction in the absence of organic phase reaction, a regime analysis of the organic phase reaction in the absence of the aqueous phase reaction, and a regime analysis of reactions in both phases. The reactions have been found to be first order with respect to H2A and second order with respect to L-IBTA and HP-β-CD. Competitive extraction of H2A enantiomers with HP-β-CD and L-IBTA has great influence on the extraction process. With increase of HP-β-CD concentration, high enantioselectivity was obtained, but the extraction rate decreases. 相似文献
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This paper reports on determination of the intrinsic reaction kinetics in reactive extraction of α-cyclohexyl-mandelic acid (α-CHMA) enantiomers with hydroxypropyl-β-cyclodextrin (HP-β-CD) in a modified Lewis cell, in which HP-β-CD dissolved in 0.1 mol/l NaH2PO4/H3PO4 buffer solution was selected as chiral extractant. α-CHMA enantiomers were extracted from an organic phase to an aqueous phase in the extraction module. The theory of extraction accompanied by chemical reactions has been used to obtain the intrinsic kinetics of this extraction module. The different factors affecting the extraction rate, such as agitation speed, interfacial area, initial enantiomers concentration in an organic phase as well as HP-β-CD concentration in an aqueous phase were separately studied. The experimental results demonstrate that the extraction reaction kinetics is fast. The reactions between α-CHMA enantiomers and HP-β-CD in a stirred cell fall in regime 3 and are first order with respect to α-CHMA enantiomers and second order with respect to HP-β-CD with forward rate constants of 6.9×10−2 m6/(mol2 s) and 2.5×10−2 m6/(mol2 s) for S-α-CHMA and R-α-CHMA, respectively. These data will be useful in the design of the extraction process. 相似文献
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Domańska U Pelczarska A Pobudkowska A 《International journal of molecular sciences》2011,12(4):2383-2394
Guest-host complex formation of three drug derivatives of anthranilic acid, mefenamic acid, niflumic acid, and flufenamic acid with 2-hydroxypropyl-β-cyclodextrin (2HP-β-CD) in aqueous solutions was investigated using "Phase solubility study" with UV-vis spectrophotometry. Solubility of sparingly soluble drugs has been improved by addition of 2HP-β-CD at two temperatures 298.15 K and 310.15 K and two pH values 2 and 7. The influence of different 2HP-β-CD concentration on solubility of drugs at different pH and temperatures has been investigated. The 2HP-β-CD-drug complex stability constants (K(s)), and dissociations constants (K(d)), as well as the thermodynamic parameters of reaction, i.e., the free energy change (ΔG), the enthalpy change (ΔH) and the entropy change (ΔS), were determined. The experimental data indicated formation of 1:1 inclusion complexes, which were found effective binders increasing the solubility of drugs. 相似文献
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通过溶液共混法制备青藤碱-羟丙基-β-环糊精包合物,纯化后利用体视显微镜观察包合物的结晶形态,通过差热示重扫描、红外光谱、X射线粉末衍射及核磁共振波谱等方法对包合物进行性质分析,并通过相溶解度法计算包合物的包合常数。结果表明,青藤碱、羟丙基-β-环糊精包合后性质发生明显变化,羟丙基-β-环糊精能显著增加青藤碱的溶解度,包合物中青藤碱与羟丙基-β-环糊精包合分子个数比为1∶1,其包合常数为150.0 L/mol,反应的吉布斯自由能为-12.41 kJ/mol。 相似文献
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