三种缝线材料对人肺腺癌细胞A549增殖和细胞周期的影响

Objective: The interaction of cell and medical biomaterial is one of the significant factors to affect clinical application of medical biomaterial. This research is to investigate three of suture lines how to affect the proliferation and cell cycle of lung adenocarcinoma cell A549 in vitro. Methods: Three of suture lines were respectively cultivated with lung adenocarcinoma cell A549, after of 72 hours, we detected absorptions of each group by MTT method in order to reflect the proliferation of lung adenocarcinoma cell A549, and also examined percentage of G1 period cells and S period cells of each group by flow cytometry. Results: Different of suture lines had different effects on the proliferation and cell cycle of lung adenocarcinoma cell A549 (P ＜ 0.05). The effect of absorbent suture line was the strongest on the proliferation and cell cycle of lung adenocarcinoma cell A549, the effect of chorda serica chirurgicalis was medium, and the effect of slide wire was poor. Different length of each suture line had different effects on the proliferation and cell cycle of lung adenocarcinoma cell A549 (P ＜ 0.05).Conclusion: Three of suture line materials have different effects on the proliferation and cell cycle of lung adenocarcinoma cell A549, with dose-effect relationship.     

血管平滑肌细胞的增殖因素及机制

血管平滑肌细胞(VSMC)增殖已成为目前心血管疾病研究领域里的热点,VSMC增殖、迁移及凋亡在冠状动脉粥样硬化(AS)、冠状动脉搭桥术、经皮冠状动脉介入治疗(PCI)术后血管再狭窄的发生和发展过程中起重要作用.本文作者对VSMC增殖及促增殖因素和机制进行综述.     

A1N抑制的取向硅钢工艺

本发明介绍一种隔离涂层,运用这种含有有机或无机酸的酰胺或酰亚胺的隔离涂层制作具有单取向性且导磁导μ_(10)≥1850高斯/奥斯特的电磁硅钢的工艺。实验室试验已表明:有些AlN抑制的取向硅钢在含有氮气保护的气氛中进行最终退火时,取得了好的磁性;而同样的退火在氢气保护下进行时则不能得到满意的磁性。推测这是     

树突状细胞联合CIK细胞应用于晚期恶性实体瘤治疗的临床疗效观察

Objective: The aim of this study was to observe the therapeutic effect of cytokine induced killer (CIK) cells in combination with dendritic cells (DCs) on advanced solid carcinoma patients. Methods: Isolated peripheral blood mononuclear cells (PBMCs) from 110 advanced solid tumor patients. Added granulocyte-macrophage colony-stimulating factor (GM-CSF),tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) to adherent cells to induce DCs, and sensitized DCs with antigens of autologous tumor cells or extrinsic tumor cell lines. Cultured suspending cells with interferon-γ(IFN-y), interleukin-2 (IL-2) and CD3 monoclonal antibody (CD3 McAb) to prepare CIK cells, then co-cultured with DCs. After analyzing the phenotype and checking tumor markers and immune function, the autologous CIK cells and DCs were transfused into the cancer patients.Results: Forty-two patients with measurable nidus, 2 achieved complete remission (CR), 9 partial remission (PR) and 15 stable disease (SD), while 37 patients with immeasurable nidus, 25 had efficient response. The tumor markers and immune function both improved significantly compared with those before treatment. Conclusion: DCs and CIK cells combinational treatment is safe and effective on advanced solid carcinoma and provide a new and efficacious immunity therapeutic methods for the cancer patients.     

辛伐他汀联合阿糖胞苷对K562细胞增殖与凋亡的影响

目的 探讨辛伐他汀(SV)联合阿糖胞苷(Ara-C)对K562细胞增殖与凋亡的影响.方法 不同浓度SV和Ara-C单用或者联合处理K562细胞,对照组为K562细胞.药物作用24、48、72 h后收集细胞,分别观察各组细胞形态,采用MTT法检测不同组别细胞的生长抑制率,采用流式细胞术检测细胞早期凋亡率、细胞坏死比例.结果 SV联合Ara-C组与单药组相比细胞形态明显有核固缩现象,且可见凋亡小体形成,并且随着处理时间的增加,抑制率也增大.其中15 μmol/L SV联合20 μmol/LAra-C的细胞抑制作用最为显著,72 h细胞抑制率为(72±1)%,明显高于15 μmol/L SV组的(45±2)%和20μmol/LAra-C组的(44±0)%(P＜0.01),表现为协同抑制作用(24、48 h金氏Q值为1.24和1.19).流式细胞术检测发现20、15和10μmol/LSV组K562细胞早期凋亡率AnnexinV明显高于对照K562细胞(P＜0.01),而且随着时间延长和剂量的增大早期凋亡率也增加(P＜0.05).20和15 μmol/LSV组早期凋亡率均高于10 μmol/LSV组,而前两者之间差异无统计学意义(P＞0.05).晚期凋亡细胞率(PI)各组中差异均无统计学意义(P＞0.05).结论 SV体外抑制K562细胞增殖及诱导细胞凋亡,SV与Ara-C具有协同作用,增加了K562细胞对化疗药物的敏感性.15 μmol/L可能为SV体外最佳作用浓度.     

MTAP、CDKN2A和CDKN2B在弥漫大B细胞淋巴瘤中的表达及其临床意义

目的 研究弥漫大B细胞淋巴瘤(DLBCL)MTAP、CDKN2A和CDKN2B基因的表达及其临床意义.方法 以实时定量聚合酶链反应(PCR)方法检测40例DLBCL及19例淋巴结反应性增生组织中MTAP、CDKN2A和CDKN2B基因的表达情况,结合临床特征进行分析,并进行随访.结果 DLBCL组MTAP、CDKN2A和CDKN2B基因表达水平较淋巴结反应性增生组降低,差异有统计学意义(P值分别为0.024、0.044和0.047);三者表达均与Ann Arbor临床分期相关(P值分别为0.004、0.001和0.027);与患者的性别、年龄、淋巴结外病变累及、ECOG体力评分、骨髓累及、血清乳酸脱氢酶水平均无明显相关(均P＞0.05).其中MTAP与CDKN2A基因表达情况还与B症状(P值分别为0.003和0.028)和国际预后指数(IPI)相关(P值分别为0.001和0.011).此外,生存分析结果显示,MTAP、CDKN2A和CDKN2B基因表达水平与患者总生存期相关(P值分别为0.022、0.019和0.042).结论 MTAP、CDKN2A和CDKN2B基因在DLBCL中呈低水平表达,与疾病进展和患者预后有关,可作为反映其生物学行为和评估患者临床疗效的分子标志物.     

pⅢA/N2期非小细胞肺癌患者外科治疗的预后分析

Objective: The aim of the study was to identify prognostic factors in non-small-cell lung cancer (NSCLC) with N2nodal involvement. Methods: A retrospective analysis of disease free survival and 5-year survival for NSCLC patients who underwent primary surgical resection without neoadjuvant chemotherapy were performed. Between January 1998 and May 2004, 133 patients were enrolled. Several factors such as age, sex, skip metastasis, number of N2 lymph node stations, type of resection, histology, adjuvant therapy etc., were recorded and analyzed. SPSS 16.0 software was used. Results: Overall 5-year survival for 133 patients was 32.33%, 5-year survival for single N2 station and multiple N2 stations sub-groups were 39.62% and 27.50% respectively, and 5-year survival for cN0-1 and cN2 sub-groups were 37.78% and 20.93% respectively.COX regression analysis revealed that number of N2 station (P= 0.013, OR: 0.490, 95% Cl: 0.427-0.781) and cN status (P =0.009, OR: 0.607, 95% Cl: 0.372-0.992) were two favorable prognostic factors of survival. Conclusion: Number of N2 station and cN status were two favorable prognostic factors of survival. In restrict enrolled circumstances, after combined therapy made up of surgery and postoperative adjuvant therapy have been performed, satisfied survival could be achieved.     

面向P2P网络的DDoS攻击抑制方法

提出了一种分布式的基于对P2P网络中各节点进行分级的DDoS攻击抑制方法,采集了多个能分别反映当前节点本身或周围节点网络状况的评级因子,并通过不确定性推理确定当前节点分级值.分级值决定转发率.使用线性分类作为丢包策略对需发送数据包进行分组、丢弃,以降低误报率.仿真实验表明该方法能够有效抑制P2P网络上的DDoS攻击,提高整个网络抗攻击弹性.     

喷洒CaCl_2溶液抑制烧结矿RDI的时效性研究

对喷洒CaCl2溶液以抑制烧结矿RDI的时效性进行了研究,并对其失效原因进行分析。     

Inhibitory Effects of Natural Compound Alantolactone on Human Non-small Cell Lung Cancer A549 Cells

Alantolactone is a natural compound identified from the roots of Inula helenium L. that has multiple bio-activities. We examined its inhibitory effects on human non-small cell lung cancer(NSCLC) A549 cells. The antiproliferative effect of alantolactone on A549 ceils was investigated via MTT[3'-(4,5dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide] assay and its apoptosis-inducing effect was determined by Hoechst staining and flow cytometry. We found that alantolactone significantly inhibited the proliferation of A549 cells and induced morphological changes typical for apoptosis. Flow cytometry analysis indicates dose-dependent cell cycle retardation at G0/G1 and S stages. The results indicate that alantolactone could be an attractive small-molecular natural compound for further development as a therapeutic drug against NSCLC.     

华蟾素注射液对人肝癌HepG-2细胞增殖及周期的影响

Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and cell cycle of human hepatoma HepG-2 cells. Methods: Cell proliferation was assessed by MTT assay, cell cycle distributionwas detected by the flow cytometry (FCM). The expression of Cyclin A, CDK2 mRNA levels were examined by RT-PCR.Quantitative colorimetric assay was used to analyze Cyclin NCDK2 activity in HepG-2 cells. Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose- and time-dependent ways; FCM analysis showed Cinobufacini injection induced cell cycle arrest at S phase; RT-PCR assay showed Cinobufacini injection down-regulated Cyclin A, CDK2expression at mRNA levels; Quantitative colorimetric assay showed Cinobufacini injection deceased Cyclin A/CDK2 activity in HepG-2 cells. Conclusion: Cinobufacini injection can inhibit human hepatoma HepG-2 cells growth, induce cell apoptosis and induce cell cycle arrest at S phase, the mechanism of which might be partly related to the down-regulation of Cyclin A,CDK2 mRNA expression and inhibition of Cyclin A/CDK2 activity.     

膀胱癌组织中MMP-2与TIMP-2 mRNA的表达及临床意义

Objective:The aim of the study was to investigate the expressions of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of MMP-2 (TIMP-2) mRNA in transitional cell carcinomas of bladder and discuss their clinical significances.Methods:Using RT-PCR and real time quantitative PCR (RQ-PCR) technique, the expressions of MMP-2 and TIMP-2 mRNA of 45 cases of bladder carcinoma (tumor group) and 10 cases of normal bladder tissue (control group) were analyzed. Results:MMP-2 and TIMP-2 were not expressed in control group. MMP-2 was expressed in 30 cases tumor samples and TIMP2 was expressed in 26 cases. The expression of MMP-2 mRNA in non-muscle invasive bladder cancers and grade I cancers significant (P < 0.05). The expression of MMP-2 in recurrent patients was higher than that in incipient patients. TIMP-2 mRNA expression decreased with grades and stage. The expression of TIMP-2 in non-muscle invasive bladder cancers and statistical difference between two groups (P < 0.05). TIMP-2 expression in incipient patients was higher than that in recurrent patients, but the difference was not significant (P > 0.05). Conclusion:These results suggest that MMP-2 and TIMP-2 play an important role in the invasion step of transitional cell carcinoma of bladder. MMP-2 may become a new approach to the diagnosis of bladder carcinoma.     

2-(2-吡啶偶氮)-5-二乙氨基酚固相萃取光度法测定水中痕量铜

在pH4．0HAc—NaAc缓冲介质中，溴化十六烷基三甲基铵(CTMAB)存在下，2—(2—吡啶偶氮)—5—二乙氨基酚(PADAP)与铜反应生成2：1稳定络合物，该络合物用watersPorapak^e Sep—Park固相萃取小柱富集．乙醇洗脱后光度法测定，可测定水样中μg/L级的铜。方法用于几种饮用水中铜的分析，结果令人满意。     

2-(5-碘-2-吡啶偶氮)-5-二甲氨基苯胺与钯显色反应的研究及其应用

探讨了2-(5-碘-2-吡啶偶氮)-5-二甲氨基苯胺(5-I-PADMA)与钯(Ⅱ)的显色反应。实验表明,在0.6 mol/L高氯酸介质中,5-I-PADMA与钯(Ⅱ) 反应形成摩尔比为1∶1的蓝色稳定络合物,该络合物最大吸收峰位于613 nm处。钯(Ⅱ)含量在0~0.6 μg/mL范围内符合比尔定律,线性回归方程为△A=0.828 4 ρ(μg/mL)- 0.001 6,相关系数r=0.999 9,表观摩尔吸光系数为8.82 × 10⁴ L·mol^-1·cm^-1。方法应用于钯分子筛和矿样中钯的测定,结果与原子吸收光谱法一致,相对标准偏差(n=6)为1.2%～1.4%。     

2-(5-溴-4-甲基-2-吡啶偶氮)-5-二甲氨基苯胺与镍显色反应的研究

研究了新试剂2-(5-溴-4-甲基-2-吡啶偶氮)-5-二甲氨基苯胺(5-Br-4-CH₃-PADMA)与镍的显色反应,建立了分光光度法测定镍的新方法。结果表明,在 pH 值为 4.2~6.0 的 HAc-NaAc 缓冲溶液中,在阴离子表面活性剂十二烷基硫酸钠(SDS)存在下,镍与 5-Br-4-CH₃-PADMA 形成稳定的组成比为1∶2的紫色配合物,其最大吸收波长位于 567 nm 处。镍的质量浓度在0～0.40 μg/mL范围内遵守比尔定律,校准曲线的线性相关系数r=0.999 2,表观摩尔吸光系数ε=1.22×10⁵ L·mol^-1·cm^-1。以硫脲和氟化铵做掩蔽剂可消除Cu²⁺、Fe³⁺ 和Pd²⁺等离子的干扰。方法用于铝合金中微量镍的测定,结果的相对标准偏差(RSD, n=6)为0.58%~0.98%,并与火焰原子吸收光谱法测定值一致。     

（2—苯并噻唑）—3—（4—哨基苯）—三氮烯与锌的显色反应的研究及应用

研究了新试剂(2 苯并噻唑) 3 (4 硝基苯) 三氮烯(BTNPT)与Zn 的反应。在阳离子表面活性剂溴化十六烷基三甲基铵(CTMAB)存在下,于pH10 14的缓冲溶液中,该试剂与锌可形成稳定的橙红色的络合物,其表观摩尔吸光系数为2 7×105,锌含量在0～12μg/25mL范围内符合比尔定律。     

1-(2-吡啶偶氮)-2-萘酚光度法测定钒钛磁铁矿中痕量镓

利用1-(2-吡啶偶氮)-2-萘酚(PAN)与镓反应生成红色络合物的特性,建立了光度法测定钒钛磁铁矿中镓的方法。实验表明:于25 mL比色管中,依次加入2.0 mL KHP-HCl缓冲溶液(pH 3.2)、4.0 mL 0.5 g/L PAN溶液、3.0 mL 95%乙醇,用水定容后反应10 min,用1 cm比色皿,于545 nm处采用分光光度法进行测定,镓的质量浓度在0.05～3 μg/mL范围内符合比尔定律,方法检出限为0.033 μg/mL,表观摩尔吸收系数ε=3.0×10⁴ L·mol^-1·cm^-1。将GBW 07226a钒钛磁铁矿精矿一号和GBW 07224钒钛磁铁矿原矿一号样品经氢氧化钠和过氧化钠碱熔、水浸取、过滤、酸化还原铁等步骤后,采用乙酸丁酯萃取、水反萃取,使镓与大量基体元素分离,再采用方法对样品中痕量镓进行测定,结果与认定值一致,相对标准偏差(RSD,n=3)小于15%。     

应用V-H_2O_2-TADAP三元配合物光度法测定钒

在pH5.6～6.0时钒(V)与2-(2-噻吩偶氮)-5-二乙氨基酚(TADAP)形成λmax位于590nm的红色配合物。当加入过氧化氢时则形成组成为1:1:1的三元配合物,反应pH值向酸性方向移动,适宜的pH值为1.10～1.50,λmax移至600nm,显色反应具有较高的灵敏度(ε=5.1×10_4L·mol~(-1)·cm~(-1)和良好的选择性,方法可用于钢铁和其他样品中钒的直接分光光度测定。