The sensitivity of the blood lymphocytes in stomach cancer patients to met-enkephalin, dopamine and serotonin

Sensitivity of blood lymphocytes to the stimulating effect of met-enkephalin and dopamine was shown to decrease at late stages of the gastric cancer (IIIB-IV) or in the case of metastases to other organs, while to the inhibitory effect of serotonin it increased as compared to early stages (I-IIA). Using specific blockers of opioid, dopamine and serotonin receptors it was shown that these effects were mediated through stimulation of respective type receptors.     

Surface phenotype and suppressor function of the blood lymphocytes in multiple myeloma

Surface phenotype of peripheral blood lymphocytes was studied in 18 patients with myeloma by combined rosette-forming and immunofluorescent methods. A high content of B lymphocytes with the identical type of immunoglobulin, and a decreased level of T lymphocytes were revealed. The increased activity of spontaneous and Con A-induced suppressor activity and cytochemical changes of some intracellular hydrolytic enzymes were found in lymphocytes of patients with myeloma.     

Effect of indomethacin on the immunological indices of patients with inoperable stomach cancer

Immunoregulatory T-lymphocytes (T mu- and T gamma-lymphocytes) and circulating immune complexes were studied in inoperable patients with the stomach carcinoma of stages III-IV. It is established that the absolute number of T-lymphocytes, mainly of T-helpers/inductors increases after the administration of indomethacin and 5-fluorouracil with indomethacin. The T mu/T gamma-lymphocyte ratio increases and approaches the control values. The level of the circulating immune complexes tends to be normalized. The main effect of indomethacin is determined by the effect of T-lymphocytes on immunoregulatory subpopulations.     

Cyclic adenosine monophosphate content in the lymphocytes of cancer patients

Examinations have been carried out on 83 cancer patients (aged 34-72), 24 patients with atherosclerosis (aged 38-68) and 34 healthy persons (aged 20-69). The cAMP concentration decrease with age in lymphocytes of healthy persons is confirmed. This index in patients with lung carcinoma, breast carcinoma in remission and in patients with atherosclerosis was identical with that of healthy persons, but in patients with breast and corpus uteri carcinomas without metastases it was higher than in healthy persons of the similar age. The amount of cAMP in lymphocytes is compared with the magnitude of the blast transformation and with the amount of cholesterol in blood and lymphocytes in examined persons. The cAMP level in lymphocytes is observed to increase in the process of hyperlipidemia disappearance in cancer patients and in those with atherosclerosis.     

δ—氨基酮戊酸—光动力疗法对红斑狼疮患者外周血中激活T淋巴细胞的影响

本文探讨了δ-氨基酮戊酸—光动力疗法 (ALA -PDT)对活动期红斑狼疮 (SLE)患者外周血中激活T淋巴细胞的影响。实验采用免疫荧光单标记—流式细胞仪检测 12例活动期SLE患者外周血中CD71阳性淋巴细胞百分比 ,观察其在ALA -PDT前后的变化。发现ALA -PDT能显著降低CD71阳性淋巴细胞百分比 ,ALA -PDT前CD71阳性细胞百分比为 12 31±1 40 ,ALA -PDT后继续培养 18小时下降至 6 5 9± 0 95 ,有非常显著的差异 (P <0 0 1)。本试验证实ALA -PDT能降低活动期SLE外周血中激活T淋巴细胞百分比 ,即可能抑制活动期SLE外周血中激活的T淋巴细胞的活性     

Production of interleukin-2 by peripheral blood lymphocytes from patients with soft tissue sarcomas

Interleukin-2 (IL-2) production of phytohemagglutinin-stimulated peripheral blood lymphocytes (PBL) was studied in 9 healthy subjects and 19 patients with soft tissue sarcomas. Mean IL-2 production by PBL in 19 patients was significantly diminished as compared with the control. Surgery leads to an increase of IL-2 production, however, the levels observed in the control do not restore completely.     

Immunosuppressive activity of the blood serum of patients with cancer of the corpus uteri

In 40 patients with endometrial carcinoma of I-IV stage reaction of suppression of migration and adhesion of leukocytes (RSML and RSAL) in response to antigen prepared from the same tumour and in the presence of autoserum of blood of these patients, was studied. The index of antibody-forming cells (IAFC) and percentage of rosette-producing cells (RPC) in mice spleen after their infusion before immunization by sheep erythrocytes blood serum of the mentioned patients were also investigated. It was ascertained, that in all cases with using of autoserum of patients with endometrial carcinoma, especially on late stages, significant diminution of positive RSML and RSAL and decrease of IAFC and RPC take place. It was concluded that blood serum of patients with endometrial carcinoma gives evidence of immunosuppressive activity at late stages of the process.     

Use of the polyamine test in the joint administration of 5-fluorouracil and indomethacin in stomach cancer patients

Excretion of polyamines (spermine, spermidine and putrescine) with the urine was studied in patients with carcinoma of the stomach of the III-IV stage after intravenous injection of 5-fluorouracil and indomethacin. A 40% increase of the polyamine level in the urine was observed at the beginning and towards the end of drug infusion course as well as a decrease of these indices at the top point of chemotherapy and towards the end of it. It is emphasized that an increase of the polyamine amount following the separate use of 5-fluorouracil is less expressed than following combined use of 5-fluorouracil and indomethacin.     

人外周血淋巴细胞体外刺激活化的原子力显微镜研究

本文利用原子力显微镜,对分离的人外周血淋巴细胞和经多克隆刺激剂佛波醇酯(PDB)和离子霉素(Ion)刺激活化的淋巴细胞形态和膜表面超微结构的形态学变化进行了研究。结果表明:淋巴细胞在受刺激后形貌发生明显变化,尺寸变大,同时细胞表面超微结构也趋复杂,出现免疫突触。这些形态变化是与淋巴细胞的功能相适应的,AFM是研究淋巴细胞形态学变化和细胞结构功能之间关系的有力工具。     

窄谱中波紫外线联合卤米松对寻常型白癜风患者皮肤炎症及黑素细胞功能的影响

探讨窄谱中波紫外线(NV-UVB)联合卤米松对寻常型白癜风患者皮肤炎症及黑素细胞(MC)功能的影响.方法:选取2017年1月至2018年12月本院100例寻常型白癜风患者,依据随机数字表分为A组和B组,每组50例,B组给予0.05％卤米松治疗,A组在此基础上联合NV-UVB治疗,比较两组皮肤炎症[白介素(IL)-2、I...     

Distribution of one of the ascitic fluid antigens of ovarian cancer patients in malignant tumors of the stomach and intestines

The indirect immunofluorescent technique was used to study the distribution of the ascitic fluid antigen (AgD) on histological sections from surgical specimens of gastric and colon tumours from proximal to distal lines of resection. AgD was found in gastric tumours exceptionally in the tumour area and in colon cancer--both in the tumour stroma and in surrounding histologically normal mucosa.     

Ultrastructural characteristics of NKHI+- and CD8+-subpopulations of large granule-containing lymphocytes in the blood of patients with Hodgkin's disease

Ultrastructure of NKH1+ and CD8+ subpopulations of large granular lymphocytes (LGL) obtained by the flow cytometry method using corresponding monoclonal antibodies was studied in patients with Hodgkin's disease. Electron microscopy has identified different changes in ultrastructure of LGL, especially of osmiophilic granules. The results indicate significant changes in the functional activity and defect of LGL in blood at early stages of the disease.     

Intensity of the synthesis of RNA precursors in blood lymphocytes of cattle infected with leukemia virus

The peripheral blood lymphocytes of the cattle with lymphatic leukemia experimentally induced by BLV and of the animals from the same group with persistent lymphocytosis show the lower level of the pre-RNA-synthetic activity per cell when compared with the lymphocytes of healthy cattle (as evaluated by autoradiography with 3H-uridine as a precursor). At the same time the RNA-synthetic activity in the population as a whole increases as a result of growth of the RNA synthesizing cells (a labelling index), which is confirmed by enhancement of DNA-dependent RNA-polymerase activities and 3H-uridine in vitro incorporation into lymphocytes revealed by the liquid scintillation counting method.     

Detection of viral particles in the blood lymphocytes of a patient with T-cell acute lymphoblastic leukemia

The electron microscopic examinations of the short-term cultures of mononuclear cells from patients with T-cell acute lymphoblastic leukemia were conducted for the detection of viral particles. The viral particles which correspond in their morphology to C type retroviruses are observed in the intercellular space of the culture.     

Quantitative immunoenzyme determination of the lactoferrin and alpha-lactalbumin in the blood serum of cancer patients

An enzyme-linked immunosorbent assay (ELISA) has been used for quantitative determination of lactoferrin (Lf) and alpha-lactalbumin (alpha-La) in human blood serum. The mean concentration of Lf in normal serum was 265 +/- 21 ng/ml and that of alpha-La did not exceed 5 ng/ml. The increased Lf level (above 450 ng/ml) was found in 16 of 30 (53%) patients with gastrointestinal cancer, in 11 of 25 (44%)--with lung cancer, in 13 of 42 (31%)--with breast cancer and more rarely--in other cancer patients. The increased alpha-La level (above 5.0 mg/ml) was found in 5 of 42 patients with breast cancer and very rarely--in other cancer patients. A conclusion is drawn that serological measurements of Lf and alpha-La were of no diagnostic value for breast cancer.     

腹腔镜胆囊切除术治疗肝硬化合并的胆囊疾患

目的：探讨肝硬化合并胆囊病变患者行腹腔镜胆囊切除术（LC）的临床特点。方法：对照研究同一时期36例肝硬化伴胆囊病变和41例不伴肝硬化的胆囊病变患者行LC术的治疗经过及结果。结果：在肝硬化患者组,LC完成34便,因术中出血中转2例,术后发生并发症3例,两组在手术时间、术中出血量和术后住院时间均有显著性差异（P〈0.05）;两组均无严重并发症和手术死亡。结论：肝硬化患者行LC难度较大,但安全可行,疗效满意,注重围手术期处理,掌握手术特点是LC成功的关键。     

Local despiralization of DNA in lymphocytes of patients with chronic lympholeukemia

DNA isolated from blood lymphocytes of 43 patients with chronic lymphoid leukemia was studied for the content of local despiralized sites by the kinetic formaldehyde method. The patients were classified into three groups: before, during and after chemotherapy by cytostatic drugs (cyclophosphamide or chlorambucil). It is found that DNA from leukosis lymphocytes contain more despiralized sites as against to DNA from normal lymphocytes and that after chemotherapy a degree of DNA despiralization decreases.     

Characteristics of the carbohydrate structure of alpha 2-macroglobulin in stomach cancer

A comparative study was made for alpha 2M preparations obtained from plasma of patients with gastric carcinoma and healthy individuals. No significant differences were found in trypsin-binding activity of alpha 2M, in isoelectric focusing microheterogeneity and in binding with lectins of Limulus polyphemus hemolymph. The study of alpha 2M-phytohemagglutinin (PHA) binding in sera of patients with gastrointestinal carcinoma and healthy people revealed highly significant differences. The alpha 2M preparations from the gastric carcinoma patients had less affinity for PHA. The data suggest the existence of an abnormal carbohydrate structure of alpha 2M in gastric carcinoma patients.     

甘露聚糖肽(力尔凡)治疗胃癌的临床研究

目的:观察甘露聚糖肽联合化疗治疗胃癌的疗效。方法:104例胃癌患者随机分成两组。观察组52例,应用甘露聚糖肽联合化疗药物治疗,甘露聚糖肽每日静点20mg,连用2周。对照组52例,单用化疗药物治疗。两组化疗用药方案相同。评价治疗前后两组病人的血象、血清肿瘤标志物水平、病人的不良临床表现(乏力、食欲减退、恶心、呕吐、手脚麻木)改善情况。结果:①观察组血象(WBC、RBC、HB、PLT)在治疗前后无明显变化P>0.05;而对照组治疗后血象下降较治疗前有显著差异P<0.05:两组治疗后血象变化具有统计学意义P<0.05。②观察组治疗前后血清肿瘤标志物CA-199、CEA、CA-153水平变化不显著,与对照组相仿,两组治疗后血清肿瘤标志物CA-199、CEA、CA-153水平变化无统计学意义P>0.05。③观察组治疗后不良临床症状改善明显,生活质量明显提高,与对照组比较有显著差异P<0.05;结论:甘露聚糖肽具有良好稳定化疗病人血象、改善病人全身状况、抑制肿瘤细胞繁殖、提高患者生活质量的作用。     

Effects of recombinant interleukin-2 on several characteristics of functional activity of lymphocytes from the lymph nodes regional to tumor and mononuclear cells of peripheral blood in cancer patients

The level of endogenous production of IL-2 by lymphocytes of lymph nodes regional to tumour and by mononuclear cells of peripheral blood, proliferative response of these cell to recombinant IL-2, as well as a modifying influence of autologous serum and actively proliferating bioptats of autologous tumours on enumerated parameters have been studied in cancer patients (tumours of the head and neck and locomotor system). Regional IL-2-dependent immunotherapy of malignant tumors with obligatory preliminary testing for individual sensitivity of the tumor bioptat to the influence of the RIL-2 and RIL-2 activated lymphocytes is shown to be promising.