猪胸腺提取物在化妆品中的抗氧化功效研究

近年来,生物活性肽中的抗氧化活性肽能够清除自由基、保护细胞抵御氧化应激损伤,从而达到抗衰老的生物活性逐渐被证实.目前,关于猪胸腺提取物抗氧化能力的研究鲜见报道.本次试验以北京修正制药有限公司提供的猪胸腺提取物为研究对象,通过生物体外的DPPH自由基与ABTS+自由基清除试验,探讨其抗氧化能力.结果表明,猪胸腺提取物的浓...     

莲花提取物体外抗氧化和保湿功效评价

考察莲花提取物体外抗氧化和保湿功效。利用MTT法检测莲花提取物的细胞毒性,以及莲花提取物对2,2-二(4-叔辛基苯基)-1-苦肼(DPPH)自由基清除活性和对HaCaT细胞水通道蛋白3(AQP3)和丝聚蛋白(FLG)基因表达的影响。结果显示,莲花提取物无细胞毒性且具有极好的DPPH自由基清除活性,能够有效促进天然保湿因子AQP3和FLG的表达,以编号为61040的莲花提取物效果最好。     

(动物)胸腺提取物细胞毒性、抗氧化能力及美白功效探究

本文主要探讨由北京修正制药有限公司生产的(动物)胸腺提取物(提取来源：幼猪胸腺)应用于化妆品中细胞毒性、抗氧化能力及美白功效的探究。使用四甲基偶氮唑盐(MTT)法评价其细胞毒性,使用超氧自由基清除法评价其抗氧化能力,使用酪氨酸酶活性抑制法评价其美白能力。结果显示(动物)胸腺提取物试验范围内,细胞增殖率均大于100%,无细胞毒性;随着(动物)胸腺提取物含量的增加,其对超氧阴离子自由基的清除能力逐渐上升;在0.625%以下,表现出一定的酪氨酸酶抑制能力。(动物)胸腺提取物作为化妆品原料,无细胞毒性,具有抗氧化、助渗透的皮肤调理剂。     

化妆品功效评价(Ⅱ)——保湿功效宣称的科学支持

概述了皮肤的保湿机制与常见保湿剂的作用机理,介绍了物理化学法、细胞生物法、三维重组皮肤模型替代法、主观评估、客观仪器评估等保湿化妆品功效评价方法,提供了保湿功效评价的新思路,展望了保湿化妆品功效评价的发展方向。     

保湿化妆品功效评价与发展展望

保湿化妆品越来越被人们所重视,其安全性和功效性评价自然成为消费者和生产厂家所关注的重点。本文从科学角度介绍了保湿化妆品的保湿原理,主要活性成分,作用机制,并重点介绍了国内外对保湿化妆品功效评价的进展情况,包括保湿活性成分的检测和分析、重量法、Corneometer仪器电容测试法、Skincon仪器电导测试法、Tewameter仪器经表皮水分流失值测试法、IR测试,以及一些其它的保湿功效评价方法,等等,同时展望了保湿化妆品的未来发展趋势,为我国保湿化妆品产品的发展提供了参考。     

一款含鲜奶面霜的保湿功效评价研究

以主客观结合方法对一款含鲜奶面霜进行单次和长期使用后的保湿功效研究,评价鲜牛奶作为保湿剂在护肤品中的保湿性能。选择30名18～55周岁女性受试者为研究对象,采用随机双盲安慰剂对照的实验设计,通过无创仪器检测并记录实验组和对照组受试者使用对应产品0 min、30 min、1 h、3 h、6 h、14 d、28 d时脸颊处角质层水分含量、经皮失水率值及VISIA-CR全脸拍摄图像,并收集受试者主观评价结果,借助SPSS数据统计软件对实验结果进行统计分析。结果显示,与不含鲜奶的基质相比,含鲜奶面霜对面部角质层水分含量的提高、经皮失水率的改善、肌肤泛红等问题的改善都具有显著作用,说明鲜牛奶作为保湿剂添加到护肤品中,具有良好的补水保湿、增强皮肤屏障功能的作用。     

一款美白产品的功效研究

制备一种美白精华乳,并对其美白和保湿功效进行研究。选择24名女性志愿者,按要求在前臂内侧持续使用美白精华乳8周,测试皮肤相应位置的MI值、L*值、ITA°值、皮肤角质层水分含量的变化情况。结果表明,使用化妆品8周后,皮肤的MI值、L*值、ITA°值和水分含量与初始值之间具有显著性差异(P<0.05),表明产品具有一定的美白和保湿功效。     

橙皮提取物的抗氧化作用研究

用Na_2S_2O_3—I_2滴定法测定了橙皮提取物在油脂中的抗氧化作用,结果表明,不同溶剂的提取物均表现出一定的抗氧化活性;以橙皮乙醇提取物为实验对象,测定其与数种增效剂在油脂中的协同抗氧化作用,和用Ce~(3+)荧光分光光度法测定了其对Fenton反应产生的羟自由基·OH的清除率,结果表明橙皮乙醇提取物与抗坏血酸的协同抗氧化作用最好,对Fenton反应产生的·OH具有清除作用。     

文献资料法支持化妆品保湿功效宣称的建议

《化妆品功效宣称评价规范》规定文献资料可用于化妆品保湿功效宣称的支持,但对于文献资料应满足何种条件尚无具体规定。通过汇总和分析作用机理、理化试验、细胞试验、皮肤模型、动物试验、人体试验6种类型的文献,就支持保湿功效的文献资料所应符合的要求提出了建议,同时就驻留类产品的文献资料在淋洗类化妆品中的应用,以及专利权利要求书中试验数据的规范使用展开了讨论。目前的建议是基于行业现状提出的,今后应该与时俱进地加以修改完善,使化妆品的功效宣称依据更加科学和充分。     

灵芝提取液的发酵优化及保湿性能评价

以灵芝提取液为原料,在纳豆芽孢杆菌、酿酒酵母菌和开菲尔菌这3种菌株中筛选出适宜的发酵菌株,结果发现选择纳豆芽孢杆菌作为菌种较为合适。以保湿率为评价指标,通过单因素及正交试验对灵芝提取液发酵工艺条件进行了优化。实验结果表明：碳源果糖6%、氮源氯化铵8.5%、接种量12%、发酵温度31℃、发酵周期32 h为发酵的最佳工艺条件,在此条件下得到的灵芝提取发酵液8 h保湿率为11.51%,保湿能力强于阳性对照组SK-Ⅱ和10%甘油,且优于灵芝提取液和空白培养基。     

Antioxidant Activity of a Red Lentil Extract and Its Fractions

Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtained using acetone-water (1:1; v/v) as the mobile phase. Phenolic compounds present in the crude extract and its fractions demonstrated antioxidant and antiradical activities as revealed from studies using a β-carotene-linoleate model system, the total antioxidant activity (TAA) method, the DPPH radical-scavenging activity assay, and a reducing power evaluation. Results of these assays showed the highest values when tannins (fraction 2) were tested. For instance, the TAA of the tannin fraction was 5.85 μmol Trolox^® eq./mg, whereas the crude extract and fraction 1 showed 0.68 and 0.33 μmol Trolox^® eq./mg, respectively. The content of total phenolics in fraction 2 was the highest (290 mg/g); the tannin content, determined using the vanillin method and expressed as absorbance units at 500 nm per 1 g, was 129. There were 24 compounds identified in the crude extract using an HPLC-ESI-MS method: quercetin diglycoside, catechin, digallate procyanidin, and p-hydroxybenzoic were the dominant phenolics in the extract.     

芦荟防晒保湿乳液的制备

探讨了防晒机理,利用物理防晒和化学防晒相结合的方法,制备了一种含芦荟和TiO2的保湿防晒乳液;主要讨论了反应温度、芦荟和TiO2含量对产品保湿性、防晒性的影响。实验表明,所制备的芦荟防晒保湿乳液符合相关行业标准,具有较好的防晒、保湿性能。     

Antioxidant Effects of the Ethanol Extract from Flower of Camellia japonica via Scavenging of Reactive Oxygen Species and Induction of Antioxidant Enzymes

The aim of this study was to investigate the antioxidant properties of the ethanol extract of the flower of Camellia japonica (Camellia extract). Camellia extract exhibited 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) scavenging activity in human HaCaT keratinocytes. In addition, Camellia extract scavenged superoxide anion generated by xanthine/xanthine oxidase and hydroxyl radical generated by the Fenton reaction (FeSO(4) + H(2)O(2)) in a cell-free system, which was detected by electron spin resonance spectrometry. Furthermore, Camellia extract increased the protein expressions and activity of cellular antioxidant enzymes, such as superoxide dismutase, catalase and glutathione peroxidase. These results suggest that Camellia extract exhibits antioxidant properties by scavenging ROS and enhancing antioxidant enzymes. Camellia extract contained quercetin, quercetin-3-O-glucoside, quercitrin and kaempferol, which are antioxidant compounds.     

Antioxidant,Analgesic, Anti-Inflammatory,and Hepatoprotective Effects of the Ethanol Extract of Mahonia oiwakensis Stem

The aim of this study was to evaluate pharmacological properties of ethanol extracted from Mahonia oiwakensis Hayata stems (MOS_EtOH). The pharmacological properties included antioxidant, analgesic, anti-inflammatory and hepatoprotective effects. The protoberberine alkaloid content of the MOS_EtOH was analyzed by high-performance liquid chromatography (HPLC). The results revealed that three alkaloids, berberine, palmatine and jatrorrhizine, could be identified. Moreover, the MOS_EtOH exhibited antioxidative activity using the DPPH assay (IC₅₀, 0.743 mg/mL). The DPPH radical scavenging activity of MOS_EtOH was five times higher that that of vitamin C. MOS_EtOH was also found to inhibit pain induced by acetic acid, formalin, and carrageenan inflammation. Treatment with MOS_EtOH (100 and 500 mg/kg) or silymarin (200 mg/kg) decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared with the CCl₄-treated group. Histological evaluation showed that MOS_EtOH reduced the degree of liver injury, including vacuolization, inflammation and necrosis of hepatocytes. The anti-inflammatory and hepatoprotective effect of MOS_EtOH were found to be related to the modulation of antioxidant enzyme activity in the liver and decreases in malondialdehyde (MDA) level and nitric oxide (NO) contents. Our findings suggest that MOS_EtOH has analgesic, anti-inflammatory and hepatoprotective effects. These effects support the use of MOS_EtOH for relieving pain and inflammation in folk medicine.     

Molecular Characterization of Thymus capitellatus Extracts and Their Antioxidant,Neuroprotective and Anti-Proliferative Activities

Thymus capitellatus Hoffmanns & Link is an endemic species of the Iberian Peninsula listed as near-threatened, due to its restricted geographical distribution, occurring mainly in Portugal’s mainland. In this work, we detail for the first time T. capitellatus extracts’ phytochemical composition, as well as an evaluation of bioactivities to point out potential health benefits. Aqueous decoction (AD) and hydroethanolic (HE) extracts were obtained, both rich in flavonoids. However, quercetin-(?)-O-hexoside was identified as the main compound in T. capitellatus HE extract, while the phenolic acid rosmarinic acid was the main component of AD extracts. In addition, HE extract presents significant amounts of salvianolic acids and of the terpenoids oleanolic and ursolic acid. Both extracts showed antioxidant activity, evaluated by their capacity to scavenge ABTS and superoxide radicals, as well as an ability to prevent lipid peroxidation. AD extracts were also effective in scavenging hydroxyl and nitric oxide radicals. As potential functional foods, T. capitellatus extracts presented neuroprotective and anti-diabetic activity, in addition to time- and dose-dependent anti-proliferative activity against Caco-2 (colorectal adenocarcinoma) and HepG2 (hepatic carcinoma) cells. HE extract presented higher cytotoxicity than AD extract, and HepG2 cells were more resistant than Caco-2 cells. After 24 h exposure to HE extract, the IC₅₀ values were 330 μg/mL and 447 μg/mL for Caco-2 and HepG2 cells, respectively. T. capitellatus has potential as a functional food or as a source of bioactive molecules. These results also highlight the need to preserve species with as yet unknown molecular compositions and potential medicinal applications.     

柠檬提取液生物合成纳米硒及其抗氧化性

以柠檬提取液为原料生物合成纳米硒,借助UV-Vis技术跟踪纳米硒的形成过程,采用XRD对纳米硒化学组成和结构进行了表征,通过SEM观察其表面形貌,用粒径仪测定了纳米粒径,利用FTIR分析了纳米硒表面与生物分子的化学作用,并对制备的纳米硒清除超氧阴离子自由基、羟基自由基和1,1-二苯基-2-三硝基苯肼(DPPH)自由基的能力进行了测试。结果表明,当pH=10时,柠檬提取液料液比为〔1∶5(g∶m L)〕可还原50 mmol/L的亚硒酸生成无定形态纳米硒,反应时间不同,所得纳米硒粒径也不同,反应1 h时合成纳米硒粒径介于78.8～122.4 nm、反应2 h时粒径介于164.2～295.3 nm,呈球形,分散性较好;柠檬提取液既是合成纳米硒的还原剂又是稳定剂,其生物分子与纳米硒表面的化学作用是纳米硒稳定存在的基础;纳米硒的抗氧化活性与质量浓度呈正相关,抗氧化性顺序为:VC>小尺寸纳米硒>大尺寸纳米硒。     

Hepatoprotective and Antioxidant Effects of Licorice Extract against CCl(4)-Induced Oxidative Damage in Rats

Licorice has been used in Chinese folk medicine for the treatment of various disorders. Licorice has the biological capabilities of detoxication, antioxidation, and antiinfection. In this study, we evaluated the antihepatotoxic effect of licorice aqueous extract (LE) on the carbon tetrachloride (CCl(4))-induced liver injury in a rat model. Hepatic damage, as reveled by histology and the increased activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) activities, and decreased levels of serum total protein (TP), albumin (Alb) and globulin (G) were induced in rats by an administration of CCl(4) at 3 mL/kg b.w. (1:1 in groundnut oil). Licorice extract significantly inhibited the elevated AST, ALP and ALT activities and the decreased TP, Alb and G levels caused by CCl(4) intoxication. It also enhanced liver super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), Glutathione S-transferase (GST) activities and glutathione (GSH) level, reduced malondialdehyde (MDA) level. Licorice extract still markedly reverses the increased liver hydroxyproline and serum TNF-α levels induced by CCl(4) intoxication. The data of this study support a chemopreventive potential of licorice extract against liver oxidative injury.