Hydrogel–silver nanoparticle composites: A new generation of antimicrobials

Design of consistant and eco‐friendly methods for the synthesis of silver nanoparticles (AgNPs) is a significant forward direction in the field of application of antibacterial bionanotechnology. One among the available options is hydrogel templates, which are highly useful to achieve this goal. This investigation involves the development of poly(acrylamide)/poly(vinyl alcohol) hydrogel–silver nanocomposites (HSNCs) to achieve AgNPs of ～2–3 nm size in gel networks. The nanocomposite synthesis process is quite convenient, direct, and very fast, and the obtained hydrogel AgNP composites can be used for antibacterial and wound dressing applications. All the nanocomposite aqueous solutions have shown absorption peaks at 420 nm in UV–visible absorption spectrum corresponding to the Plasmon absorbance of AgNPs. X‐ray diffraction spectrum of the HSNC exhibited 2θ values matching with silver nanocrystals. Transmission electron microscopy images of nanocomposites represent discrete AgNPs throughout the gel networks in the range of 2–3 nm. The developed nanocomposites were evaluated for antibacterial application on E. coli. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

甲型H1N1流感病毒裂解疫苗动物体内的安全性和免疫原性

目的评价甲型H1N1流感病毒裂解疫苗在动物体内的安全性和免疫原性。方法按《中国药典》三部(2005版)方法进行异常毒性试验。将小鼠随机分为7组,每组10只,实验组小鼠分别经小鼠后腿胫前肌肌肉注射不同血凝素含量(15、30、45μg/0.5 ml)的甲型H1N1流感病毒裂解疫苗,同时设疫苗对照组(相应规格的季节性H1N1流感病毒裂解疫苗)及阴性对照组(PBS),注射剂量均为0.1 ml/只,间隔21 d加强免疫1次,分别于初免后0、21、28、35及42 d采集眼静脉血,分离血清,检测血凝抑制(hemagglutination inhibition,HI)抗体水平。结果异常毒性试验结果符合《中国药典》三部(2005版)判定标准。与阴性对照组比较,初免后21、28、35、42 d,各剂量实验组小鼠血清抗体水平均明显升高,21 d即上升到较高水平,28 d达到峰值(P均<0.01);初免后0、21、28、35、42 d,除35 d外,均高于相应剂量疫苗对照组(P均>0.05)。初免后21 d,15、30、45μg/0.5 ml剂量实验组小鼠血清中HI抗体阳转率分别为80%、90%和90%,HI抗体保护率分别为100%、90%和90%,与相应剂量疫苗对照组相比,差异均无统计学意义(P均>0.05);初免后28、35、42 d,15、45μg/0.5 ml剂量实验组HI抗体阳转率维持在80%以上,30μg/0.5 ml剂量实验组HI抗体阳转率略低,而各剂量实验组HI抗体保护率均在90%¹00%之间。结论甲型H1N1流感病毒裂解疫苗在昆明小鼠中具有良好的安全性和免疫原性。     

Alloferon and Zanamivir Show Effective Antiviral Activity against Influenza A Virus (H1N1) Infection In Vitro and In Vivo

The use of vaccines is the most effective and reliable method for the prevention of viral infections. However, research on evaluation of effective therapeutic agents for use in treatment after infection is necessary. Zanamivir was administered through inhalation for treatment of pandemic influenza A/H1N1 in 2009. However, the emergence of drug-resistant strains can occur rapidly. Alloferon, an immunomodulatory drug developed as an NK cell activator, exerts antiviral effects against various viruses, particularly influenza viruses. Therefore, alloferon and zanamivir were administered in combination in an effort to improve the antiviral effect of zanamivir by reducing H1N1 resistance. First, we confirmed that administration of combined treatment would result in effective inhibition of viral proliferation in MDCK and A549 cells infected with H1N1. Production of IL-6 and MIP-1α in these cells and the activity of p38 MAPK and c-Jun that are increased by H1N1 were inhibited by combined treatment. Mice were then infected intranasally with H1N1, and examination of the antiviral efficacy of the alloferon/zanamivir combination was performed. The results showed that combined treatment after infection with H1N1 prevented weight loss, increased the survival rate, and improved lung fibrosis. Combined treatment also resulted in reduced infiltration of neutrophils and macrophages into the lungs. Combined treatment effectively inhibited the activity of p38 MAPK and c-Jun in lung tissue, which was increased by infection with H1N1. Therefore, the combination of alloferon/zanamivir effectively prevents the development of H1N1-mediated inflammation in the lungs by inhibiting the production of inflammatory mediators and migration of inflammatory cells into lung tissue.     

Preparation,characterization, and antibacterial activity of chitosan/silicone rubber filled zeolite,silver, and copper nanocomposites against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus

The ongoing emergence of antibiotic-resistant bacteria has become one of the biggest threats to global health and development today. Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) are important antibiotic-resistant bacteria due to their increasing resistance to a broad array of antimicrobial agents. Herein, we developed a novel antibacterial nanocomposite based on chitosan and liquid silicone rubber filled zeolite-A, Ag, and Cu nanoparticles with remarkable antibacterial activity against P. aeruginosa and MRSA. The antibacterial activity of the nanocomposite was studied by disc diffusion and broth culture methods. Besides, the mechanical properties, wetting behavior, and chemical structure of the present nanocomposite were also investigated. The results exhibited that the inhibition zone diameter of the nanocomposite for P. aeruginosa and MRSA were 40 and 27 mm, respectively. It also took approximately 1 h to inhibit the growth of the tested bacteria. The nanocomposite sample with a thickness of around 1 mm showed an elastic elongation of nearly 49% and a contact angle of roughly 120°. Thus, the present nanocomposite was found to be useful in killing and inhibiting the growth of P. aeruginosa and MRSA, and it could also be qualified as a superior elastic and hydrophobic material.     

甲型H1N1流感病毒裂解疫苗在动物体内诱导中和抗体水平及其过敏原性

目的评价甲型H1N1流感病毒裂解疫苗在动物体内诱导的中和抗体水平及其过敏原性。方法将昆明小鼠随机分为7组,分别经腹腔注射3批甲型H1N1流感病毒裂解疫苗(剂量分别为15、30、45μg/0.5 ml)、3批季节性H1N1流感病毒裂解疫苗(剂量分别为15、30、45μg/0.5 ml)和PBS(阴性对照),每只0.5 ml,间隔21 d加强免疫1次,分别于免疫前、初次免疫后21 d及加强免疫后14 d采血,分离血清,采用固定病毒-稀释血清法检测小鼠血清中和抗体滴度。将豚鼠随机分为4组,分别经腹腔注射上述3批甲型H1N1流感病毒裂解疫苗和PBS(阴性对照)各0.5 ml,隔日注射1次,共3次,第1次注射后第14和21 d,分别经静脉注射同一批号的疫苗1.0 ml攻击,观察豚鼠的过敏反应。结果初次免疫后21 d,甲型H1N1流感病毒裂解疫苗15、30、45μg剂量组免疫小鼠血清中和抗体滴度分别为免疫前和阴性对照组的47.9、113.5和319.9倍,且呈剂量依赖性;加强免疫后14 d,甲型H1N1流感病毒裂解疫苗各剂量组中和抗体滴度比初次免疫后21 d均明显升高,也呈剂量依赖性,且均高于与相应剂量的季节性H1N1流感病毒裂解疫苗。甲型H1N1流感病毒裂解疫苗各剂量组豚鼠均无过敏反应发生。结论甲型H1N1流感病毒裂解疫苗在昆明小鼠体内可诱导产生保护性中和抗体,对豚鼠无过敏反应。     

不同裂解工艺制备的甲型H1N1流感病毒裂解疫苗的透射电子显微镜观察

目的透射电子显微镜观察不同工艺制备的甲型H1N1流感病毒裂解疫苗形态,筛选最佳裂解工艺,为疫苗的大规模生产提供参考依据。方法应用透射电镜对4种不同裂解工艺制备的甲型H1N1流感病毒疫苗进行形态观察,采用中和试验分析其免疫原性,异常毒性试验分析其安全性。结果透射电镜观察显示4种样品均无完整病毒,样品1裂解程度最大,囊膜破裂,多数为病毒碎片;样品2大多数囊膜未破损,缺少纤突;样品3较完整的病毒颗粒略小(约30~40 nm),纤突清晰;样品4大多数病毒颗粒保留着纤突,但纤突已不整齐清晰。样品2组小鼠中和抗体滴度最低,而样品1、3、4组较高,与样品2组相比,差异明显,其中以样品3组最高;样品2组小鼠、豚鼠体重增加不明显,而样品1、3、4组小鼠、豚鼠体重增加明显,与样品2组相比差异显著,其中以样品3组最明显。结论电镜观察可直观反映疫苗裂解程度;3种裂解工艺均可用于疫苗生产,其中样品3具有较好的免疫原性和安全性,其工艺可作为最佳裂解工艺用于后续疫苗生产。     

H1N1亚型流感病毒M1基因重组杆状病毒的构建及鉴定

目的构建H1N1亚型流感病毒M1基因重组杆状病毒,并进行鉴定。方法 PCR扩增H1N1亚型流感病毒(A/PR/8/34)全长M1基因,与pFastBacdua(lpFBD)载体连接,构建重组杆状病毒转移载体pFBD-M1,转化含有Bacimd和Helper质粒的DH10Bac感受态细胞,获得骨架质粒rBacmid-M1,将其转染sf9昆虫细胞,获得重组杆状病毒rBac-M1。采用噬斑形成法检测病毒滴度,PCR法检测M1基因的插入,间接免疫荧光、Western blot和ELISA法检测M1蛋白的表达。结果重组杆状病毒骨架质粒rBacmid-M1经PCR鉴定证实构建正确;第3代rBac-M1病毒滴度为3×108pfu/ml;感染rBac-M1的sf9细胞经PCR扩增可见3 300 bp的条带,间接免疫荧光检测可见特异性绿色荧光,Western blot检测可与鼠抗流感病毒(PR8)和鼠抗M1蛋白(PR8)多克隆抗体发生特异性反应,ELISA检测M1蛋白可与鼠抗流感病毒(PR8)多克隆抗体发生特异性反应,具有良好的反应原性。结论已成功构建了H1N1亚型流感病毒M1基因重组杆状病毒,为进一步研究流感病毒M1的功能及新型流感疫苗开发奠定了基础。     

影响禽流感病毒(H3N2/H4N6)感染机制的生物信息学分析

目的通过生物信息学分析禽流感病毒WDK/ST/27/03(H3N2)和Dk/ST/472/03(H4N6)HA、NA氨基酸序列,进一步探讨影响禽流感病毒感染血液肿瘤细胞株K-562和HL-60的机制。方法 RT-PCR扩增WDK/ST/27/03(H3N2)和Dk/ST/472/03(H4N6)HA、NA基因,经1%琼脂糖凝胶电泳鉴定,对纯化的PCR产物进行测序。在GenBank中选取登录的H3N2和H4N6序列,利用在线生物信息工具ClustalW2(http://www.ebi.ac.uk/Tools/msa/clustalw2/)对这些序列和WDK/ST/27/03(H3N2)和Dk/ST/472/03(H4N6)HA、NA氨基酸序列进行比对分析。结果 RT-PCR扩增产物的大小与预期相符。生物信息学分析显示,WDK/ST/27/03(H3N2)HA的裂解位点是QNR*GLFG,Dk/ST/472/03(H4N6)HA的裂解位点是KAAR*GLFG,此位置的氨基酸无变化。HA的226位氨基酸为Gln(Q),228位氨基酸为Gly(G),具有禽流感病毒HA受体结合位点的特征,影响受体结合的位点98Y、136S、152W、183H、190E、194L、222W、225G、227S未见变异。NA活性位点及影响酶活的位点均无变异,但WDK/ST/27/03(H3N2)NA在接近N-末端的茎部61⁷9位缺失19个氨基酸,致使NA茎部变短,且丢失2个潜在的糖基化位点。结论 WDK/ST/27/03(H3N2)的NA茎部缺失突变可能是造成其吸附、繁殖和复制能力低于Dk/ST/472/03(H4N6)的一个因素。     

A novel efficient RhB absorbent of Mo2N/MoO2 composite nanofibers for wastewater treatment

Mo₂N/MoO₂ composite nanofibers have been prepared via an electrospinning and controlled nitridation process. The composite nanofibers exhibit a highly efficient Rhodamine B (RhB) absorption behavior with a rate constant of 0.153 g min⁻¹ mg⁻¹, which is about 20 times of the commercial-activated carbon material. Furthermore, the nanofibers show stable absorption activity after recycled by an environmental friendly procedure for four times. The excellent absorption performance of Mo₂N/MoO₂ composite nanofibers demonstrates a promising application of Mo₂N-related materials as an absorbent for wastewater treatment.     

Synthesis of carbon nanotube/titanate nanotube composites with photocatalytic activity for H2S oxidation

Composites of carbon nanotubes – titanate nanotubes (MWNTs/ TNTs) – were successfully synthesized by the hydrothermal reaction process. The morphology of the MWNTs/TNTs composites was observed by transmission electron microscopy. Fourier transform infrared spectroscopy was used to characterize the molecular interactions between TNTs and MWNTs in the composites. The X-ray diffraction of TNTs and MWNTs/TNTs composites exhibited the existence of titanate phase (100), (102), (020) and (422). The charge recombination of MWNTs/TNTs composites was examined by photoluminescence spectrum. The photocatalytic activity of TNTs and MWNTs/TNTs composites was evaluated on the basis of the model oxidation reaction of H₂S.     

H5N1流感病毒疫苗株(NIBRG-14)在MDCK-G1细胞上的遗传稳定性

目的分析H5N1流感病毒疫苗株(NIBRG-14)在MDCK-G1细胞上的遗传稳定性。方法将H5N1种子毒株在MDCK-G1细胞上传代,收获病毒液作为P2病毒,继续传至P15,每隔5代[即P1(原代)、P5、P10、P15]进行测序。分别以含不同浓度(1、2、4μg/mL)TPCK-trypsin的培养基及不同病毒接种MOI(1、0. 1、0. 01、0. 001、0. 000 1、0. 000 01)在MDCK-G1细胞上培养P1 H5N1病毒,检测血凝滴度,计算CCID50,确定最适MOI及TPCK-trypsin浓度。将P1和P15 H5N1病毒接种鸡胚,收获尿囊液,经Sepharose 4 Fast Flow凝胶柱层析法纯化病毒后,进行电镜观察;采用培养法和指示细胞培养法(DNA染色法)检测支原体。结果 H5N1流感病毒疫苗株(NIBRG-14)在MDCKG1细胞传代过程中血凝滴度增加,从1∶128升至1∶1 024,P1、P5、P10和P15 H5N1种子病毒8条基因序列(PB1、PB2、PA、HA、NP、NA、M、NS)经DNAMAN比对结果一致。H5N1种子病毒株在MDCK-G1细胞传代的最适TPCKtrypsin浓度为4μg/mL,最适MOI为0. 001。两种方法检测P1、P15 H5N1种子病毒株均未被支原体感染。结论H5N1种子病毒株在MDCK细胞中传至P15时,病毒滴度增加但基因序列未发生改变,表明H5N1种子病毒株在MDCK-G1细胞中传代具有一定的遗传稳定性。     

静注甲型H1N1流感人免疫球蛋白的研制

目的研制高效价静注甲型H1N1流感人免疫球蛋白。方法用甲型H1N1流感疫苗(简称甲流)对固定供血浆者按疫苗说明书免疫后2周开始采集原料血浆,采用血凝抑制法检测原料血浆和制品的甲流抗体效价;采用柱层析法从高效价甲流免疫血浆中提取富含IgM免疫球蛋白的样品,模拟低温乙醇蛋白分离法工艺从小量混合血浆分离IgG样品,测定血浆和提取样品的甲流抗体效价,分析甲流中和抗体效价与IgG、IgM含量的对应关系,判断甲流中和抗体的免疫球蛋白类型;按本公司静脉注射人免疫球蛋白(Intravenous immunoglobulin,IVIG)生产工艺制备静注甲型H1N1流感人免疫球蛋白,并与普通IVIG及相应合并血浆的甲流抗体效价进行比较。结果筛选到甲流抗体效价≥320 HU/ml的合格血浆9 866袋,合格率达33.5%,其中抗体效价≥640 HU/ml的血浆占合格血浆的49%,血浆质量符合《中国药典》三部(2010版)"血液制品生产用人血浆规程"要求;甲流抗体效价与IgG含量呈相应比例关系,而与IgM含量无关,甲流中和抗体的免疫球蛋白类型以IgG为主;制备的甲流人免疫球蛋白制品的甲流中和抗体效价达2 560 HU/ml,为普通IVIG的197倍,其他质量指标符合《中国药典》三部(2010版)"静注人免疫球蛋白制检规程"要求。结论成功研制了静注甲型H1N1流感人免疫球蛋白,在甲流疫情得到有效控制的情况下,仍具有战略储备意义。     

抗静电和电磁屏蔽木材/金属复合材料的研究进展

介绍了具有电磁屏蔽和抗静电功能的木材/金属复合材料的制造方法及试验结果。     

以MDCK为基质的H5N1流感全病毒灭活疫苗的制备及其免疫学评价

目的利用生物反应器培养技术大规模制备以MDCK细胞为基质的H5N1流感全病毒灭活疫苗,并进行免疫学评价。方法利用7. 5 L生物反应器,以5 g/L微载体培养MDCK-G1细胞,当细胞密度达到1. 5×10⁶或2. 5×10⁶个/mL时,以0. 001、0. 000 1、0. 000 01 MOI接种H5N1流感病毒,每12 h取样检测流感病毒血凝滴度,并观察细胞形态;利用40 L生物反应器进行放大培养,收获病毒液后进行纯化。以不同剂量的细胞基质和鸡胚基质流感疫苗免疫BALB/c小鼠及攻毒,检测免疫原性。结果当细胞密度达1. 5×10⁶个/mL时,以0. 000 1 MOI接种H5N1流感病毒48 h后,流感病毒滴度可达1∶1 024。纯化后病毒液杂蛋白去除率为91. 2%,回收率为87. 9%,总蛋白与HA比值低于4. 5,符合《中国药典》三部(2015版)相关规定。各剂量组细胞基质疫苗血凝抑制试验中和抗体滴度略高于鸡胚基质疫苗,其中10μg剂量组差异无统计学意义(P> 0. 05),0. 1μg剂量组差异有统计...     

Microwave Absorption of SiC/HfCxN1−x/C Ceramic Nanocomposites with HfCxN1−x‐Carbon Core–Shell Particles

The dielectric properties of high‐temperature stable single‐source precursor‐derived SiC/HfC_xN_1?x/C ceramic nanocomposites are determined by microwave absorption in the X‐band (8.2–12.4 GHz) at room temperature. The samples synthesized at 1700°C, denoted as SiC/5HfC_xN_1?x/C‐1700°C and SiC/15HfC_xN_1?x/C‐1700°C ceramics, comprising ≈ 1.3 and ≈ 4.2 vol% HfC_xN_1?x, respectively, show enhanced microwave absorption capability superior to hafnium‐free SiC/C‐1700°C. The minimum reflection loss of SiC/5HfC_xN_1?x/C‐1700°C and SiC/15HfC_xN_1?x/C‐1700°C are ?47 and ?32 dB, and the effective absorption bandwidth amount to 3.1 and 3.6 GHz, respectively. Segregated carbon, including graphitic carbon homogeneously dispersed in the SiC matrix and less ordered carbon deposited as a thin film on HfC_xN_1?x nanoparticles, accounts for the unique dielectric behavior of the SiC/HfC_xN_1?x/C ceramics. Due to their large reflection loss and their high chemical and temperature stability, SiC/5HfC_xN_1?x/C‐1700°C and SiC/15HfC_xN_1?x/C‐1700°C ceramics are promising candidate materials for electromagnetic interference applications in harsh environment.     

Electrochemical detection of avian influenza virus H5N1 gene sequence using a DNA aptamer immobilized onto a hybrid nanomaterial-modified electrode

A sensitive electrochemical method for the detection of avian influenza virus (AIV) H5N1 gene sequence using a DNA aptamer immobilized onto a hybrid nanomaterial-modified electrode was developed. To enhance the selectivity and sensitivity, the modified electrode was assembled with multi-wall carbon nanotubes (MWNT), polypyrrole nanowires (PPNWs) and gold nanoparticles (GNPs). This electrode offered a porous structure with a large effective surface area, highly electrocatalytic activities and electronic conductivity. Therefore, the amount of DNA aptamer immobilized onto the electrode was increased while the accessibility of the detection target was maintained. The biosensor is based on the hybridization and preferred orientation of a DNA aptamer immobilized onto a modified electrode surface with its target (H5N1 specific sequence) present in solution. It is selective for the H5N1 specific sequence, and the signal of the indicator was approximately linear to log(concentration) of the H5N1 specific sequence from 5.0 × 10⁻¹² to 1.0 × 10⁻⁹ M (R = 0.9863) with a detection limit of 4.3 × 10⁻¹³ M. These studies showed that the new hybrid nanomaterial (MWNT/PPNWs/GNPs) and the DNA aptamer could be used to fabricate an electrochemical biosensor for gene sequence detection. Furthermore, this design strategy is expected to have extensive applications in other biosensors.     

Transport processes of hydrated ions at polymer/oxide/metal interfaces: Part 1. Transport at interfaces of polymer coated oxide covered iron and zinc substrates

Localised electrochemical and spectroscopic techniques were jointly applied for the evaluation of hydrated ion transport processes along polymer/oxide/metal interfaces. In situ Scanning Kelvin Probe (SKP) studies of the local interfacial potentials of organically coated oxide covered zinc and iron substrates were performed in humid nitrogen atmospheres. They were supported by ex situ small spot X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectroscopy (ToF-SIMS) analysis of the interfacial ion distribution. Based on the experimental results it is concluded that also in atmospheres of strongly reduced oxygen partial pressure at which no corrosive delamination takes place, a negatively charged layer of adsorbed hydroxide ions determines ion transport processes along interfaces between polymer films and oxide covered metals. No ion transport was observed for zinc substrates while hydrated cations were selectively transported along the polymer/iron interface. The reduction of oxygen molecules on the highly reactive iron oxide surface is assumed to be responsible for the generation of adsorbed interfacial hydroxide ions. On the other hand such oxygen reduction induced hydroxide formation in humid nitrogen atmospheres with strongly reduced oxygen partial pressure does not seem to take place on oxide covered zinc. The variation of free volumes at the polymer/substrate interface did not lead to a principal change of this phenomenon.     

用于分离DMF/H2O体系的Me-silicalite-1渗透汽化分子筛膜的制备

采用二次生长法制备了用于渗透汽化分离DMF/H2O体系的杂原子取代Me-silicalite-1/α-Al2O3(Me=Co、Fe)分子筛复合膜,通过X射线衍射(XRD)、扫描电镜(SEM)、傅里叶红外光谱(FT-IR)和紫外漫反射(UV-Vis)等方法对分子筛膜进行表征,结果表明金属离子进入分子筛骨架。渗透汽化实验显示,金属离子掺杂的silicalite-1/α-Al2O3分子筛复合膜,具有较好的分离性能且优先透过有机胺,有利于工业中DMF溶剂的回收和利用。随着操作温度升高,分离因子减小,渗透通量增加。40℃分离质量分数为5%的DMF/H2O混合液时,Co-silicalite-1/α-Al2O3分子筛膜和Fe-silicalite-1/α-Al2O3分子筛膜的分离因子分别为4.4和2.9,渗透通量分别为0.66和0.84 kg·m-2·h-1。     

A TPD study of H2 ON Ru/SiO2 and Ru-Cu/SiO2

In H₂ TPD from Ru/SiO₂, two desorption peaks were observed. Both exchanged H for D in sequential dosing experiments. These hydrogen adsorption states were also found for Ru-Cu/SiO₂, along with another, higher temperature state at 400–500 K. This last state was neither exchangeable with nor replaceable by deuterium subsequently dosed at 150 K. The three chemisorption states are attributed to hydrogen held at the interface between Ru and SiO₂ (< 300 K), adsorbed on Ru particles (310–380 K), and held at the Ru-Cu interface (> 400 K).     

A facile microwave enhanced synthesis of sulfur-containing 5-membered heterocycles derived from 2-mercaptobenzothiazole over ZnCl 2/DMF and antimicrobial activity evaluation

An efficient and extremely fast procedure for the synthesis of 4-thiazolidinones 4a–j by the reaction of arylidene-[(2-benzothiazolylthio)-acetamidyl] 3a–j with thioglycolic acid in DMF in the presence of a catalytic amount of anhydrous ZnCl ₂ under microwave irradiation is described. A considerable increase in the reaction rate has been observed with better yield in microwave technique. All the compounds have been screened for their antifungal activity against Candida albicans (ATCC-64550), Candida krusei (ATCC-14243) and Candida parapsilosis (ATCC-22019) and antibacterial activity against Escherchia coli (Gram?ve) (ATCC-8739), Staphylococcus aureus (Gram+ve) (ATCC-6538) and Bacillus substilis (Gram+ve) (ATCC-6633). The structures of the synthesised compounds 4a–j have been characterized on the basis of their elemental analysis and spectral data.