不同干酵母对百香果酒的发酵影响

以百香果为原料,选用6种酿酒活性干酵母发酵制备百香果酒,为百香果产业深加工与多元化利用提供技术依据。研究测定了发酵过程中百香果酒的含糖量、p H、酒精度和总酸含量变化,并结合感官评价,分析不同酵母菌对百香果酒酿造的影响,筛选出最适的活性酿酒酵母。在此基础上探讨了不同原料果汁浓度对百香果酒酿造的影响。结果发现,在发酵温度25℃、初始糖度25.6 Brix、酵母菌接种量0.1%的条件下,安琪RW活性干酵母对百香果酒的发酵能力强且感官评分最高(89分),且在原料果汁浓度为50%时百香果酒品质最佳,酒体呈淡黄澄清透亮,具有百香果酒特有的香味,酸甜可口,口感柔和。     

野木瓜果酒专用醇备的筛选

野木瓜中富含齐墩果酸,有保护肝脏的作用。饮用野木瓜酿制的果酒可缓解酒精对肝脏的伤害。为选育适合野木瓜果酒发酵的酵母,对野木瓜果实中的天然酵母、安琪葡萄酒活性干酵母、安琪黄酒活性干酵母进行了发酵力、发酵速度等方面的比较筛选。试验结果表明,从野木瓜果实中分离的A2酵母,对野木瓜果酒发酵具有优良的酿造特性,发酵力高,发酵速度快,发酵后的果酒酒体澄清,色泽浅黄,口味协调,香味纯正,是野木瓜果酒酿造的优良天然酵母茵。     

桑葚果酒的发酵工艺研究

以桑葚为原料,安琪活性干酵母SY为发酵菌种,采用全汁发酵法酿造桑葚果酒,通过单因素和响应面试验优化,确定了桑葚果酒的最佳发酵工艺条件：起始糖度为204 g/L,活性干酵母接种量为1.4 g/L,不添加偏重亚硫酸钾,起始pH自然,发酵温度为30 ℃,发酵时间8 d,制备得到的桑葚果酒的酒精度可达到9.3%vol,残总糖18.48 g/L,总酸8.12 g/L。该条件下,桑葚果酒酒液澄清透亮,颜色纯正,酒香果香浓郁,酸甜适口,口感醇厚,风味独特,感官评分为88.7分。     

红树莓果酒主发酵工艺的优化

本文对二氧化碳浸渍法酿造红树莓果酒的主发酵工艺进行了系统的研究。以野生红树莓为原料,安琪高活性葡萄酒用干酵母为发酵菌种,残糖质量分数、酒精度、总酸和感官评价分数为指标;在单因素实验基础上,采用L9(34)正交优化实验,探究出红树莓果酒酿造的最佳主发酵工艺为:pH2.7、发酵温度22℃、初糖质量分数23%、酵母接种量4%。在此工艺条件下主发酵6d,得到红树莓果酒:残糖10.11g/L(以葡萄糖计),酒精度12.0%vol,总酸17.22g/L(以柠檬酸计),感官评分为75.83±3.06;红树莓果酒呈酒红色,澄清透明,有光泽,味纯正爽怡,有清雅果香与和谐酒香。     

早酥梨酒酿造中葡萄酒酵母的选择研究

以甘肃的早酥梨为原料进行发酵研究,通过考察不同酿酒酵母对早酥梨酒发酵中可溶性固形物含量(SSC)、残糖含量、酒精度、果酒品质和感官评价等理化指标的影响,最终证明酿造早酥梨酒最适宜的菌种为葡萄酒酵母。     

自然发酵和活性干酵母发酵金秋梨酒研究

采用人工活性干酵母和自然发酵两种发酵方式发酵生产金秋梨酒。结果表明,采用安琪活性干酵母发酵,相比自然发酵,发酵期短2d,发酵后残糖低24g／L,酒精度高2．9％vol,感官评价较优,自然发酵方式成本较低。     

香蕉菠萝复合果酒发酵工艺参数的研究

分析发酵条件对香蕉菠萝复合果酒品质的影响,确定最佳工艺参数。以香蕉、菠萝为原料,以酒精度、残糖量、总酸和感官为评价指标,采用单因素和正交试验确定了发酵温度、初始糖度、pH值及酵母用量的最适水平。结果表明,复合果酒的最佳工艺参数为发酵温度24℃、初始糖度20°Bx、pH3.6、酵母接种量1.1 g/L。采用该工艺酿造出来的复合果酒感官评价最好。     

低度甜型菠萝香蕉复合果酒酿造工艺优化

实验以成熟度良好的菠萝和香蕉为主要原料,通过单因素和正交实验进行菠萝与香蕉用量配比、发酵浸渍方式、初始发酵糖度及最终残糖对低度甜型菠萝香蕉复合果酒品质影响的研究。确定了最佳工艺条件为原料菠萝与香蕉质量比为2∶1、初始发酵糖度210 g/L、保留残糖90 g/L及无浸渍发酵,最终酒度为7.1%vol。此条件下所酿低度甜型菠萝香蕉果酒酒体呈浅金黄色,澄清有光泽,果香较典型,酒体协调,余味良好。     

菠萝果酒的发酵工艺研究

通过以菠萝为主发酵原料,采用安琪活性干酵母生产菠萝果酒,发酵过程中采用正交试验,优化工艺过程中的关键性因素,最终获得了一种色、香、味、外观俱佳的菠萝酒.并通过研究得出生产菠萝酒的最佳工艺条件:即以酒精度为评价指标的最佳发酵条件组合为:发酵温度24℃,干酵母接种量0.2 g/L,菠萝汁糖度24°Bé;以感官评价为指标的最...     

番木瓜果酒发酵及其抗氧化能力分析

以番木瓜为原料,经破碎、酶解、成分调整后接种葡萄酒活性干酵母,于（22±1） ℃发酵制备番木瓜果酒。研究发酵过程中总糖、还原糖、滴定酸含量与抗氧化能力的变化,明确番木瓜果酒的理化指标与感官品质。结果表明,番木瓜果酒发酵过程中总糖、还原糖含量逐渐降低,滴定酸含量增加,总酚含量、DPPH自由基清除率和亚铁还原能力（FRAP值）降低,pH值变化无规律性。番木瓜果酒酒精度10.7%vol,残糖量4.4 g/L,滴定酸4.5 g/L,总酚含量3.0 g/L,DPPH自由基清除率63.0%,FRAP值2.33 mmol/L,属半干型果酒,抗氧化能力较强,理化与感官品质均符合相关果酒标准要求。     

面包酵母高糖发酵力与蔗糖酶活力关系的研究

本文研究了面包酵母高糖耐性与蔗糖酶活性的关系。通过对八株酵母菌株的蔗糖酶活性和高糖面团发酵力比较分析,其中ADY2蔗糖酶酶活最大,BY-6最小,分别为128.70 U/g干酵母和30.55 U/g干酵母,而在高糖面团中发酵力却是BY-6最大,ADY2最小,CO2的产生量分别为850 ccm和225 ccm,证实了较低蔗糖酶活性的面包酵母菌株具有在高糖面团中发酵力较高的特性。通过测定蔗糖酶酶活相差较大的株菌BY-6和ADY2在蔗糖模拟面团中的蔗糖消耗和葡萄糖积累曲线,结果表明ADY2不仅蔗糖消耗速度比BY-6快,且其积累葡萄糖的速度比BY-6快,同时所积累的最高葡萄糖量也比BY-6高,分别为5.89?10-2和4.50?10-2 g/mL。此外,即便是蔗糖酶酶活低且高糖面团发酵力大菌株BY-6在蔗糖模拟面团培养基中仍有较多葡萄糖积累,因此选育蔗糖酶水解生成葡萄糖速度与其利用葡萄糖速度一致或相差不大的菌株是我们选育耐高糖面包酵母菌株的一个控制靶点。     

活性干酵母在杨梅酒酿制中的应用

研究了活性干酵母应用于发酵型杨梅酒的特性。试验结果表明 ,SO2 浓度和活性干酵母的添加量显著影响杨梅酒的发酵速率和口感。文中采用了单因素发酵性能试验和比较试验。结果表明 ,当发酵用杨梅原汁SO2 质量浓度在 5 0～ 10 0mg/L ,活性干酵母的添加量在10 6 ～ 10 7/mL条件下 ,杨梅汁发酵迅速 ,得到的酒液品质与使用As 14 5 0鲜酵母发酵得到的杨梅酒相近 ,口感较佳。活性干酵母适用于杨梅果酒的酿造     

Co60诱变原生质体选育高产酒精酵母

将较高产酒精酵母制备成原生质体,经Co^60诱变后,采用四级筛选,得到高产酒精酵母菌株Co-158,其遗传性状稳定。以玉米粉糖化醪为基质发酵72h,成熟醪酒精体积分数为17～％（v／v）,残还原糖为0．0137％。Co-158菌;洙成熟醪酒精体积分数比出发菌株提高了16．34％,比ADY提高了24．48％,残还原糖含量亦远远低于出发菌株和ADY。     

酿酒ADY在黄酒生产中的应用

黄酒活性干酵母实现了酒母商品化，是酒母培养工艺的重大创新，对促进黄酒工业现代化具有重要意义。论述了黄酒活性干酵母在机制麦曲黄酒、乌衣红曲酒和红曲酒、传统黄酒、酒母扩大培养、夏季籼米黄酒生产中的应用，以及AADY在米曲生产中的应用等。     

ADY用于黄酒残糟发酵的研究报告

对黄酒生产的副产物经蒸馏后的残糟发酵制酒进行了利用活性干酵母（简称ADY）的研究，通过不同试验方案的对比，确定了利用ADY替代传统酒母进行残糟发酵的工艺路线，并确定了ADY的最佳用量。使成品酒出酒率提高2.11%，经多年的应用情况表明，成品酒质稳定，效益增加显著。     

几种面包活性干酵母发酵不加糖面团性能的比较

对市场上的5种面包活性干酵母(ADY)进行了发酵性能测定。结果表明,不同面包酵母对低糖面团与不加糖面团发酵力存在差异,其中ADY1基本无差别,ADY2的差别较小(3.2%),而ADY4(11.9%)、ADY5(9.4%)的差别较大。通过对5种酵母麦芽糖发酵速度和葡萄糖阻遏程度的分析表明,干酵母ADY1、ADY2发酵麦芽糖速度较快,葡萄糖阻遏程度较小;而ADY4、ADY5发酵麦芽糖速度较慢,葡萄糖阻遏程度较高。可见,麦芽糖发酵速度快、葡萄糖阻遏程度小的酵母品种比较适合于不加糖面团的发酵。     

Effect of active dry yeast on lactation performance,methane production,and ruminal fermentation patterns in early-lactating Holstein cows

This study was conducted to examine the effect of active dry yeast (ADY) supplementation on lactation performance, ruminal fermentation patterns, and CH₄ emissions and to determine an optimal ADY dose. Sixty Holstein dairy cows in early lactation (52 ± 1.2 DIM) were used in a randomized complete design. Cows were blocked by parity (2.1 ± 0.2), milk production (35 ± 4.6 kg/d), and body weight (642 ± 53 kg) and assigned to 1 of 4 treatments. Cows were fed ADY at doses of 0, 10, 20, or 30 g/d per head for 91 d, with 84 d for adaptation and 7 d for sampling. Although dry matter intake was not affected by ADY supplementation, the yield of actual milk, 4% fat-corrected milk, milk fat yield, and feed efficiency increased quadratically with increasing ADY supplementation. Yields of milk protein and lactose increased linearly with increasing ADY doses, whereas milk urea nitrogen concentration and somatic cell count decreased quadratically. Ruminal pH and ammonia concentration were not affected by ADY supplementation, whereas ruminal concentration of total volatile fatty acid increased quadratically. Digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, nonfiber carbohydrate, and crude protein increased quadratically with increasing ADY supplementation. Supplementation of ADY did not affect blood concentration of total protein, triglyceride, aspartate aminotransferase, and alanine aminotransferase, whereas blood urea nitrogen, cholesterol, and nonesterified fatty acid concentrations decreased quadratically with increasing ADY supplementation. Methane production was not affected by ADY supplementation when expressed as grams per day or per kilogram of actual milk yield, dry matter intake, digested organic matter, and digested nonfiber carbohydrate, whereas a trend of linear and quadratic decrease of CH₄ production was observed when expressed as grams per kilogram of fat-corrected milk and digested neutral detergent fiber. In conclusion, feeding ADY to early-lactating cows improved lactation performance by increasing nutrient digestibility. The optimal ADY dose should be 20 g/d per head.     

Rehydration of Active Dry Brewing Yeast and its Effect on Cell Viability

The use of active dry yeast (ADY) in the brewing industry is becoming increasingly attractive due to several key features, including its capacity to be stored conveniently and to be prepared rapidly for use. However, some studies have reported that undesirable effects may occur when employing lager strains in the ADY form, such as abnormal flocculation, haze formation and a less stable foam structure. These effects have been linked to reduced viability, caused by cell death during the drying and rehydration processes. It is known that the means by which yeast is rehydrated is important to maintain membrane integrity and to prevent potentially lethal damage from occurring. In order to determine the impact of rehydration conditions on yeast viability, three industrially manufactured ADY strains were examined. Each strain was rehydrated using a variety of parameters and monitored for cell viability using slide culture and a variety of brightfield and fluorescent stains.     

马铃薯雪花全粉制酒工艺优化

以马铃薯雪花全粉为原料,安琪酿酒活性干酵母(angel active dry yeast,ADY)为发酵剂,制得马铃薯酒;通过单因素和正交试验优化马铃薯酒发酵的工艺条件。结果表明:最佳发酵工艺条件为料水比1∶7.0(g/mL),发酵温度18℃,糖化酶添加量120 U/g,蛋白酶添加量4 U/g,ADY添加量为0.005%,按此工艺发酵,所得酒度为5.3%vol,总高级醇含量为103.23 mg/L。     

Identification by phenotypic and genetic approaches of an indigenous Saccharomyces cerevisiae wine strain with high desiccation tolerance

During active dry yeast (ADY) production process, cells are exposed to multiple stresses, such as thermal, oxidative and hyperosmotic shock. Previously, by analysing cells in exponential growth phase, we selected an indigenous Saccharomyces cerevisiae wine strain, namely CD‐6Sc, for its higher tolerance to desiccation and higher expression of specific desiccation stress‐related genes in comparison to other yeast strains. In this study, we performed a desiccation treatment on stationary phase cells by comparing the efficacy of two different methods: a ‘laboratory dry test’ on a small scale (mild stress) and a treatment by spray‐drying (severe stress), one of the most appropriate preservation method for yeasts and other micro‐organisms. The expression of selected desiccation‐related genes has been also assessed in order to validate predictive markers for desiccation tolerance. Our data demonstrate that the ‘mild’ and the ‘severe’ desiccation treatments give similar results in terms of cell recovery, but the choice of marker genes strictly depends on the growth phase in which cells undergo desiccation. The indigenous CD‐6Sc was ultimately identified as a high dehydration stress‐tolerant indigenous strain suitable for ADY production. This study highlights the exploitation of natural yeast biodiversity as a source of hidden technological features and as an alternative approach to strain improvement by genetic modifications. Copyright © 2017 John Wiley & Sons, Ltd.