Influence of size distribution of proteins,thiol and disulfide content in whole wheat flour on rheological and chapati texture of Indian wheat varieties

The influence of protein composition, as measured by size-exclusion high performance liquid chromatography (SE-HPLC), on rheological properties and chapati texture was investigated in the whole wheat flours of eight Indian wheat cultivars grown at a single location. Proteins were extracted using two-step procedure: extraction with buffer containing 0.5% SDS (SDS buffer), followed by sonication. The results showed that SDS buffer extracted 72–90% of the total flour protein in different varieties and 7–11% protein was extracted from the remaining residues by sonication. The proteins extracted were fractionated by SE-HPLC into large polymeric proteins (>130 kDa), small polymeric proteins (80–130 kDa) and monomeric proteins (10–80 kDa). Total polymeric protein content in the flour protein showed a significant positive correlation with dough hardness (r = 0.71, p < 0.05) and positive correlation with chapati texture (r = 0.58, p < 0.05). Of the SDS extractable polymeric proteins, large polymeric protein in flour protein had significant positive correlation to dough hardness (r = 0.89, p < 0.05) and chapati cutting force, which reflects the chapati texture (r = 0.70, p < 0.05). Protein disulfide content showed positive correlation to dough hardness (r = 0.66, p < 0.05) and texture of chapati (r = 0.58, p < 0.05) while protein thiol content showed significant negative correlation to chapati texture (r = −0.77, p < 0.05). Thus, the results indicate that high proportion of SDS extractable large polymeric protein in flour protein increases the toughness of chapati texture while flours having high thiol content decrease the toughness of chapati.     

Rheological properties of gluten-free bread formulations

In this study, the rheological properties of rice bread dough containing different gums with or without emulsifiers were determined. In addition, the quality of rice breads (volume, firmness and sensory analysis) was evaluated. Different gums (xanthan gum, guar gum, locust bean gum (LBG), hydroxyl propyl methyl cellulose (HPMC), pectin, xanthan–guar, and xanthan–LBG blend) and emulsifiers (Purawave^™ and DATEM) were used to find the best formulation for gluten-free breads. Rice dough and wheat dough containing no gum and emulsifier were used as control formulations. The rice dough containing different gums with or without emulsifiers at 25 °C showed shear-thinning behavior with a flow behavior index (n) ranging from 0.33–0.68 (except pectin containing samples) and consistency index (K) ranging from 2.75–61.7 Pa sⁿ. The highest elastic (G′) and loss (G″) module were obtained for rice dough samples containing xanthan gum, xanthan–guar and xanthan–LBG blend with DATEM. When Purawave^™ was used as an emulsifier, dough samples had relatively smaller consistency index and viscoelastic moduli values compared to DATEM. The viscoelastic parameters of rice dough were found to be related to bread firmness. Addition of DATEM improved bread quality in terms of specific volume and sensory values.     

Utilization of chestnut flour in gluten-free bread formulations

In this study, gluten-free bread formulations using chestnut and rice flours at different ratios (0/100, 10/90, 20/80, 30/70, 40/60, 50/50 and 100/0) were tested. In addition, the influence of hydrocolloid blend (xanthan-locustbean gum (LBG), xanthan-guar gum blend) and emulsifier DATEM on the rheological properties of dough formulations and quality parameters of breads were also investigated for the samples having chestnut/rice flour ratio of 10/90, 20/80, 30/70 and 40/60. Herschel-Bulkley model was found to explain the flow behavior of all dough formulations. The power-law index (n) of dough samples at 25 °C ranged from 0.52 to 0.87, the consistency index (K) of the samples ranged from 3.6 to 79 Pa sⁿ and the yield stress of the samples ranged from 4.8 to 85.9 Pa. The breads prepared with chestnut/rice flour ratio of 30/70 and containing xanthan-guar blend and emulsifier, had higher quality in terms of hardness, specific volume, color and sensory values. However, elevated levels of chestnut flour led to some deterioration in quality parameters (low volume, harder texture and darker color) regardless of gum blend and emulsifier addition.     

Shelf stability and sensory properties of flour tortillas fortified with pinto bean (Phaseolus vulgaris L.) flour: Effects of hydrocolloid addition

To flour tortillas formulations containing 25 g/100 g of pinto bean flour, 0.5 g/100 g and 0.75 g/100 g of guar gum and sodium carboxymethyl cellulose (CMC) were added and their shelf stability was studied at 4 and 25 °C over 7 days. Texture, determined instrumentally, rollability, and water holding capacity were the main parameters studied. Selected samples were evaluated by 55 participants to determine consumer acceptability. Firmness and cohesiveness were negatively affected by the addition of bean flour, however, this effect was partially overcome by the addition of hydrocolloids. Guar gum had a positive significant influence on water holding capacity and texture over time (P < 0.001), while CMC had no positive effects. Despite the instrumental texture data, which showed that bean tortillas had inferior attributes than the wheat control, consumers found the overall texture and acceptability of bean tortillas with and without guar gum on the range of “like very much” and “like moderately”, which was significantly higher than the wheat control (P < 0.01). Based on physical and sensorial properties it would appear that these foods are industrially feasible and highly acceptable by health-conscious consumers.     

Effect of hydrocolloids on the rheological, microscopic, mass transfer characteristics during frying and quality characteristics of puri

Effect of hydrocolloids such as guar gum (GG), arabic gum (AG), carrageenan (CG), locust bean gum (LBG), xanthan gum (XN), hydroxypropyl methylcellulose (HPMC) and carboxymethylcellulose (CMC) at 0.5% w/w level on rheological and quality parameters of puri from whole wheat flour was studied. Hydrocolloids like CMC, XN and HPMC increased the water absorption of puri dough, while it decreased in all other samples. The dough development time and mixing tolerance index values increased, while dough stability did not get affected. On addition of hydrocolloid, there was a reduction in the pasting temperature, while the peak viscosity, hot paste viscosity and cold paste viscosity values increased. Hardness, cohesiveness and adhesiveness properties of the puri dough increased with the addition of hydrocolloids. All the hydrocolloids used in general helped in retention of moisture in the puri and hence remained softer and pliable, while there was a significant reduction in the oil content of puri samples containing hydrocolloids. Among the different hydrocolloids used, addition of guar gum at 0.5% w/w level led to puris having improved quality characteristics to a greater extent with respect to moisture retention, lowering of oil content upon frying with softer and pliable texture and better keeping quality. The mass transfer studies confirmed that the mass transfer co-efficient values for moisture loss and oil uptake were lower in puris with guar gum than control.     

Evaluation of l-ascorbic acid oxidation on SH concentration in soy-wheat composite dough during resting period

The effect of l-ascorbic acid (l-AA) on free sulfhydryl concentration (SH) was evaluated in soy-wheat composite dough from 100-500 (g/kg) soy flour substitution for wheat flour. Raw soy flour (RSF) and physically modified soy flours (PMSF1 and PMSF2) were used for the preparation of the composite dough with wheat flour. The two physically modified soy flours were prepared by steam flushing (PMSF2) and water boiling (PMSF1) of raw soy beans before flour preparation. Using a timer, dough blends were manually mixed (at approximately 60 rpm) to dough development time after which, dough was sampled for the estimation of free SH groups. l-AA (0.05% w/w) was mixed with the dough after dough development and the dough was sampled after 1 h of resting the dough. The results showed that l-AA (0.05% w/w) acted as a reducing agent by increasing SH levels in all soy-wheat dough blends (P<0.05). After 1 h of resting, soy-wheat composite dough without l-AA had lower concentrations of SH than that with l-AA. A positive correlation was shown between soy flour concentrations and SH concentration before and after dough resting. A negative correlation existed between l-AA consumption and SH concentration for RSF-wheat, PMSF1-wheat and PMSF2-wheat doughs. The results indicated that soy flour weakened wheat flour dough by increasing SH concentration and that l-AA could have a synergistic effect on the reduction of gluten proteins and thus weakening the dough.     

Tempeh flour from chickpea (Cicer arietinum L.) nutritional and physicochemical properties

The effects of solid state fermentation (SSF) on physicochemical and nutritional properties of chickpea flour were studied. Fermented (tempeh) flour showed higher particle size index, gelatinization temperature, dispersability and resistant starch content, and lower gelatinization enthalpy and water solubility than unfermented flour. SSF increased the content of the essential amino acids (EAA) Ile, total sulphur (Met + Cys), total aromatic (Phe + Tyr), and Thr in 37, 41, 107, and 39 g kg⁻¹ protein, respectively; Trp content decreased 8 g kg⁻¹ protein. Total sulphur (EAA score = 0.87) was limiting in unfermented flour and Trp (0.93) in tempeh flour. SSP improved the in vitro and true protein digestibility (72.2–83.2% and 83.7–88.8%, respectively), protein efficiency ratio (PER, 1.59–2.31), cPER (1.54–2.21), and corrected protein digestibility (0.73–0.89). Chickpea tempeh flour may be considered for the fortification of widely consumed legume-based food products.     

Yeast leavened banana-bread: Formulation,processing, colour and texture analysis

Banana powder (BP) was added to hard-red spring wheat (HRSW) flour intended for yeast-leavened bread formulation. Five different formulations containing 10%, 15%, 20%, 25%, and 30% BP were prepared with varying amounts of base flour, while vital gluten was maintained at 25% in all blends. Based on the added BP amounts only, the prepared bread could deliver 42.87–128.6 mg potassium/30 g of bread (one regular slice) and 0.33–1.00 g of fibre. Although the dough water absorption was increased, due to BP addition, the dough mixing tolerance (MTI) decreased. The bread loaf volume was significantly higher than the control except for the 30% blend, where the loaf volume was similar to the control. Bread staling increased with BP levels due to the high sugar content but, this effect was limited to the first two days of storage. Blends exhibited darker colour due to the high sugar and protein, while the 25% and 30% blends had the lowest percent of freezable water. The amounts of acetic acid extractable proteins from the dry blends and the dough decreased with increase in BP. The linear rheological properties of the control, 10%, and 30% blends exhibited similar viscoelastic solid behaviour, where both G′ and G′′ had plateaus (G′ > G′′) and they were parallel to each other over three decades of the frequency. Blends showed higher moduli values than the control.     

Effect of guar gum on the serum lipid profile of Sprague Dawley rats

The aim of the study was to probe the hypocholesterolemic effect of legumes dietary fiber through “chapaties” a staple diet of the South Asia. Commercial wheat flour (atta) was blended with legumes i.e. lentil, chickpea and guar gum in various combinations to make composite flour for the preparation of chapaties. Maximum dietary fiber (8.85%) was observed in composite flour with 3g/100 g guar gum, which also gave whiter look and puffiness to the end product. Highest percent increase in dietary fiber (35.3%) was in flour with 3g/100 g guar gum followed by guar gum 2g/100 g (24.1%). The diets prepared from three best selected compositions along with control, were fed to male Sprague Dawley rats for 8 weeks. Lowest serum cholesterol (82.46 mg/dl) was observed in rats fed on guar gum 3g/100 g which showed a significant reduction (17.2%) as compared to control. Similarly, in case of LDL and triglycerides, guar gum 3 g/100 g showed highest decline i.e., 29.7% and 28.4% with reference to control, respectively. The present investigation suggests that five chapaties per day prepared from selected compositions provides an extra 5-8 g of dietary fiber that is helpful in lowering cholesterol in hypercholesterolemic individuals.     

A comparative study of the effects of three galactomannans on the functionality of extruded pea–rice blends

The present study evaluated the effects of three galactomannans on the physical and nutritional characteristics, and sensory acceptability of pea–rice based extruded products, targeted as nutritional snacks. A base blend of 70:30 pea and rice fortified with guar gum (GG), locust bean gum (LBG) and fenugreek gum (FG), at 5%, 10%, 15% and 20%, was extruded at pre-determined optimum processing conditions. All three gums resulted in good expanded products. Increasing the inclusion levels of gums, however, had no effect (P > 0.05) on the degree of expansion. Addition of 5% GG and LBG reduced (P < 0.05) the hardness, while the inclusion of GG and LBG at levels higher than 5%, and all inclusion levels of FG, increased (P < 0.05) the hardness of extruded products. Relative to other treatments, FG produced extrudates that were harder and crispier. The mean scores of sensory evaluation indicated that all products containing gums up to 15% were within the acceptable range. Extrusion increased (P < 0.001) the soluble fibre content and decreased the insoluble fraction; the magnitude of these changes were greater in GG and FG. The addition of 15% gums in the pea–rice blend reduced (P < 0.05) the glycaemic index to less than 55. Overall, the data suggest that all three galactomannans could be incorporated up to 15% in a pea–rice blend to develop nutritious, organoleptically acceptable, extruded snack products with low glycaemic index.     

Interaction effects of fermentation time and added asparagine and glycine on acrylamide content in yeast-leavened bread

The interaction effects of fermentation time and added asparagine and glycine on acrylamide precursors (asparagine and reducing sugars) in dough and content of acrylamide in yeast-leavened wheat bread were studied. Two experiments, with low and high levels of added asparagine (0–0.044 and 0.071–0.476 g/100 g flour, respectively), were performed. Glycine was added (0.042–0.380 g/100 g flour) only in the high asparagine addition experiment. The fermentation time, which was varied between 13 and 164 min, showed a reducing effect on acrylamide precursors in the dough in both experiments (p < 0.001). These effects of fermentation were more pronounced in the experiment with low asparagine levels, which resembled levels in ingredients. In contrast, fermentation time did not affect the content of glycine in the dough. Added asparagine increased the levels of asparagine in dough and of acrylamide in bread (p < 0.001). A strong correlation was found between the contents of asparagine in the fermented dough and acrylamide in breads at all levels of asparagine. Glycine significantly increased the colour intensity and reduced the acrylamide in bread (p < 0.001) with the latter effect being dependent on the level of asparagine.     

Evaluation of Lepidium sativum seed and guar gum to improve dough rheology and quality parameters in composite rice–wheat bread

The effects of hydrocolloids in rice–wheat flour were studied. Hydrocolloids at 0%, 0.3%, 0.6% and 1% w/w (flour basis) and guar (G), Lepidium sativum seed (L) and guar-L. sativum seed (GL) gum were tested as additives to the rice/flour in various combinations. The quality parameters for the experiment were assessed with farinography, extensography, amylography and texture profile analysis. The evaluation of dough rheology showed that water absorption, dough development time, dough stability and viscosity all increased with the addition of hydrocolloids alone or in a combination. It was demonstrated that G₁L₁ promoted the highest effect. The mixing tolerance index and gelatinization temperature decreased with an increased hydrocolloid concentration. Extensibility value for the dough that incorporated guar and L. sativum seed gum increased with increasing hydrocolloid concentration from 0.3% to 0.6% and then decreased at 1%. The water activity of all bread didn't have significant differences with increasing hydrocolloids concentration but this parameter 24 decreased during storage. Firmness decreased with increasing hydrocolloid concentration and increased with increasing storage time. The sensory evaluation by a consumer panel gave the higher score for overall acceptability to G_0.3L_0.3 and G_0.3L_0.6 samples. The results also showed that G₁L₁, G₁L_0.6 and G_0.6L₁ samples had high specific volume and porosity.     

Effect of substitution of aspartame for sucrose on instrumental texture profile of hydrocolloids gelled systems

The effect of substituting aspartame (0.04–0.16% w/w) for sucrose (5–25% w/w) on the instrumental texture profile of three hydrocolloid gelled systems: -carrageenan, gellan gum and -carrageenan/locust bean gum at three different concentrations (0.3, 0.75 and 1.2% w/w) was studied. In gellan gels hardness and chewiness increased with sucrose concentration but not so in either -carrageenan or -carrageenan/LBG gels where no changes in these parameters were detected. Addition of sucrose also produced a small but significant increase in cohesiveness in gellan gels. The effect of sucrose concentration on all texture parameters of gellan gels was higher as gellan concentration increased. Aspartame addition did not affect TPA parameters. TPA results obtained for aspartame gels did not differ from those corresponding to the unsweetened gels considered here. Summarising the above information it can be said that substituting aspartame for sucrose in this type of sweetened gel changed the textural properties and that the changes produced depended on the hydrocolloid type and concentration and on the sucrose concentration replaced.     

In vitro starch digestibility,expected glycemic index,and thermal and pasting properties of flours from pea,lentil and chickpea cultivars

In vitro starch digestibility, expected glycemic index (eGI), and thermal and pasting properties of flours from pea, lentil and chickpea grown in Canada under identical environmental conditions were investigated. The protein content and gelatinization transition temperatures of lentil flour were higher than those of pea and chickpea flours. Chickpea flour showed a lower amylose content (10.8–13.5%) but higher free lipid content (6.5–7.1%) and amylose–lipid complex melting enthalpy (0.7–0.8 J/g). Significant differences among cultivars within the same species were observed with respect to swelling power, gelatinization properties, pasting properties and in vitro starch digestibility, especially chickpea flour from desi (Myles) and kabuli type (FLIP 97-101C and 97-Indian2-11). Lentil flour was hydrolyzed more slowly and to a lesser extent than pea and chickpea flours. The amount of slowly digestible starch (SDS) in chickpea flour was the highest among the pulse flours, but the resistant starch (RS) content was the lowest. The eGI of lentil flour was the lowest among the pulse flours.     

Production of microbial exopolysaccharides in the sourdough and its effects on the rheological properties of dough

Exopolysaccharides (EPS) are exogenous microbial metabolites which are secreted mainly by bacteria and microalgae during growth. In addition to natural polysaccharides present in cereal grains flour and dough, microbial flora is usually involved in production of polysaccharide on sourdough fermentation. Total polysaccharides (microbial and flour) were extracted from sourdough and dough samples dehydrated and were added at the rate of 0%, 0.25%, 0.5%, 1%, 1.5%, 2% and 2.5% (w/w flour based) on the dough to investigate its effects on the rheological properties of the dough. Addition of polysaccharides to the dough increased the water absorption and decreased the dough softening after 20 min. Resistance to extension after 45, 90 and 135 min resting time was decreased by increasing the percentage of the added polysaccharides. Longer fermentation time for each level of polysaccharides led to greater stability. No significant differences were observed in the extensibility of dough. The overall effects of different levels of added polysaccharides resulted in a decrease in resistance to extension ratio of the samples. Energy input decreased in all cases. It seems therefore that addition of polysaccharides may be useful when bread is to be made with stronger flour and longer fermentation time is needed.     

Effects of food gums and pre-drying on fat content of fabricated fried chips

Deep-frying contributes to the unique taste and texture of fried products. However, they are low in nutritional value. Food industries actively trying to find ways to reduce the fat content while maintaining organoleptic properties of fried foods. In this work, effects of pre-drying and adding food gums on the moisture and fat contents of chips were evaluated. The chips were pre-dried for 60 and 90 min, and gellan gum, guar gum, methylcellulose and xanthan gum were added at the concentration of 0.25, 0.75, 1 and 2 % w/w. The xanthan gum was the most effective gum for fat reduction. The addition of 0.25 % w/w xanthan gum and at 90 min pre-drying reduced the fat content from 20 % (control) to 15 % w/w. The results also indicated that the reduction of moisture content after frying was not affected by the type of gums but the method of pre-drying.     

Effect of Arabic gum,xanthan gum and orange oil on flavor release from diluted orange beverage emulsion

The influence of main emulsion components namely Arabic gum (13–20% w/w), xanthan gum (0.3–0.20% w/w) and orange oil (10–14% w/w) on semi-quantitative headspace analysis of target volatile flavor compounds released from a model orange beverage (diluted orange beverage emulsion) was evaluated by using a three-factor circumscribed central composite design (CCCD). For optimization procedure, the peak area of 13 volatile flavor compounds (i.e. ethyl acetate, α-pinene, ethyl butyrate, β-pinene, 3-carene, myrcene, limonene, γ-terpinene, octanal, decanal, linalool, neral and geranial) were considered as response variables. The response surface analysis exhibited that the significant (p < 0.05) second-order polynomial regression equations were successfully fitted for all response variables except for ethyl butyrate. A satisfactory coefficient of determination (R²) ranged from 0.831 to 0.969 (>0.8) was obtained for the response variables studied. No significant (p > 0.05) lack of fit was indicated for the reduced models except for the models fitted for limonene and linalool. This observation confirmed an accurate fitness of the reduced response surface models to the experimental data. The multiple response optimizations indicated that an orange beverage emulsion containing 15.87% (w/w) Arabic gum, 0.5% (w/w) xanthan gum and 10% (w/w) orange oil was predicted to provide the minimum overall flavor release.     

Quality characteristics of biscuits prepared from finger millet seed coat based composite flour

Finger millet seed coat is an edible material and contains good proportion of dietary fibre, minerals and phytochemicals. The seed coat matter (SCM) forms a by-product of millet milling, malting and decortication industries and can be utilised as composite flour in biscuit preparation. The SCM from native, malted and hydrothermally treated millet contained 9.5–12% protein, 2.6–3.7% fat and 40–48% dietary fibre, besides 3–5% polyphenols and 700–860 mg/100 g of calcium. The biscuits prepared using the composite flour were of crisp texture and exhibited breaking strength of 1480–1690 g compared to control biscuits (1560 g). The biscuits were of mild grey colour (ΔE = 40–50) and exhibited higher protein, dietary fibre and calcium contents. The sensory evaluation of the biscuits indicated that 10% of SCM from native and hydrothermally processed millet and 20% from malted millet could be used in composite biscuit flour.     

The influence of fenugreek gum and extrusion modified fenugreek gum on bread

Fenugreek gum (extruded and non-extruded) was substituted for wheat flour at 0%, 5% and 10% (w/w) and the rheological effects and breadmaking characteristics were determined. Bread containing fenugreek gum (FG) at 5% and 10% showed volumes and texture comparable with a control bread. Quality was high due to sheeting the dough twice as well as the use of the three-fold-turn puff pastry laminating method during processing of dough, instead of the standard rounding of dough. The three-fold-turn method is believed to have caused a higher incorporation of air into the dough as well as facilitated better distribution and incorporation of the fenugreek gum into bread dough. Extruding FG also improved its solubility in bread. Fenugreek gum (FG) resulted in an increase in dough farinograph water absorption compared with the control, but extruding the gum caused an even greater increase in water absorption when compared with the non-extruded gum. The addition of FG to bread dough caused an increase in G′ and G″. Starch pasting using RVA showed an increase in peak viscosity, final viscosity, breakdown and setback in a dose-related response when compared with a control.     

Effect of salt and MTGase on the production of high quality gels from farmed sea bass

In order to obtain low salt fish products, the effects of the addition of 0.5% (w/w based on whole product, wp) MTGase and different levels (0.25%, 0.50%, 1.00% and 2.50%, w/w wp) of sodium chloride salt to heat-induced gels from farmed sea bass (Dicentrarchus labrax) trimmings were tested and compared with gels without salt and/or without MTGase.MTGase and salt addition, both, had a favourable effect on texture. However, only salt incorporation augmented water holding capacity (WHC).A synergistic effect was detected between MTGase and salt for several important textural properties, particularly, gel strength, which reached 64.3 ± 8.1 N mm, a value comparable to those of best quality surimi. Moreover, MTGase required addition of salt to the product for its effect to be felt, however only a minimal amount (0.25%, w/w wp) sufficed. In fact, the addition of MTGase enabled reduction in salt content to 1.0% (w/w wp), without significant loss of textural and overall quality.