共查询到10条相似文献,搜索用时 0 毫秒
1.
Ogura S Kaneko K Miyajima S Ohshima K Yamaguchi K Mochizuki T 《Proteomics. Clinical applications》2008,2(12):1620-1627
Proteins secreted from specific cancer cells have a high potential for use as tumor markers. We identified secreted proteins produced by 15 different carcinoma cell lines grown in serum-free medium using MS/MS. Proneurotensin/neuromedin N (proNT/NMN) was found in conditioned medium from four of seven small cell lung carcinoma cell lines but not from eight nonsmall cell lung carcinoma cell lines. These results indicate proNT/NMN has potential as a specific tumor marker of small cell lung carcinoma. 相似文献
2.
Dihazi H Müller C Asif AR Flad T Elmaouhoub A Müller GA 《Proteomics. Clinical applications》2007,1(2):200-214
For most cancers, the patient's prognosis improves dramatically if the disease is detected at an early stage. Although advancements in imaging technology have improved early detection, many cancers remain undetected until it is too late for curative intervention. We have established, for the first time, expression difference mapping analysis of whole cell proteins from renal cell carcinoma (RCC) cell lines using ProteinChip technology. A total of 20 different RCC cell lines were cultured in vitro directly on ProteinChip arrays for 24 h. Direct MS analysis of proteins from the attached cells showed identical protein profiles by all analysed RCC lines. Comparative on‐chip analysis of isolated malignant cells from native tumour specimens revealed protein patterns highly similar to those from the continuous RCC lines. However, cultured primary cortex cells showed specific protein differences. Differential protein profiling of isolated cytosolic and enriched membrane fractions from the RCC lines revealed that the protein pattern of the membrane proteins included or were identical to those of the entire cells. Proteomics analysis of the chip‐binding membrane fractions allowed the identification of three forms of galectin‐1 as potential RCC marker. ProteinChip analysis with a bound‐specific antibody certified that galectin‐1 could be an RCC marker. Immunostaining methods confirmed the overexpression of galectin‐1 in renal carcinoma in comparison to healthy tissue. 相似文献
3.
Chang JW Kang UB Kim DH Yi JK Lee JW Noh DY Lee C Yu MH 《Proteomics. Clinical applications》2008,2(1):23-32
Comparative proteome analysis was performed on the cultured media of human nontumor and malignant breast cell lines, Hs578Bst and Hs578T, respectively, in search of a serological biomarker(s) for breast cancer. Proteins in the conditioned media were separated by 2‐D PAGE and then visualized by silver‐staining. Eight proteins changed differentially by more than two‐fold were identified by MALDI‐TOF/TOF MS. Among the proteins identified, the terminal laminin‐like globular (LG3) domain of endorepellin, which was recently reported as an antiangiogenesis factor, was decreased in the cancer cell line. We confirmed the bone morphogenic protein‐1 (BMP‐1) mediated cleavage site on the N‐terminus of endorepellin LG3 fragment. This finding suggests that the LG3 fragment is specifically released by a BMP‐1 driven limited proteolytic process. The protein was also detected in plasma by Western blot analysis and selected reaction monitoring (SRM). The plasma level of the endorepellin LG3 fragment was significantly lower in breast cancer patients compared to healthy donors (p = 0.017; n = 12). The LG3 protein concentration in the control plasma was measured at approximately 3.7 pmol/mL compared to 1.8 pmol/mL in plasma from the cancer patients. We suggest that these results support the potential use of the endorepellin LG3 fragment as a new serological biomarker for breast cancer. 相似文献
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Yoshihiro Kamada Hideki Fujii Hironobu Fujii Yoshiyuki Sawai Yoshinori Doi Naofumi Uozumi Kayo Mizutani Maaya Akita Motoya Sato Sachiho Kida Noriaki Kinoshita Nobuhiro Maruyama Takayuki Yakushijin Masanori Miyazaki Hisao Ezaki Naoki Hiramatsu Yuichi Yoshida Shinichi Kiso Yasuharu Imai Norifumi Kawada Tetsuo Takehara Eiji Miyoshi 《Proteomics. Clinical applications》2013,7(9-10):648-656
6.
Proteomic analysis as a means to approach limbal stem cell biology in a search for stem cell markers
The cornea consists of three main layers: an outer surface epithelium, the stroma, and the endothelium. A clear cornea is necessary for optimal vision and is maintained and repaired from limbal epithelial stem cells located in the limbus between the cornea and the sclera. Diseases and injury may result in deficiency of the stem cells impairing their ability to renew the corneal epithelium. Patients with limbal stem cell deficiency experience chronic pain and ultimately blindness. Attempts to treat the disease are based on replacement of the stem cells by transplantation or by culturing the stem cells. We here review the proteomic techniques that so far have been used to approach characterization of limbal stem cells and markers to identify them. It is apparent that the field is in a rather inchoate state due to the scarcity and relative inaccessibility of the stem cells. However, the importance of revealing limbal stem cell biology and identifying stem cell biomarkers calls for greater use of emerging methodology. Strategies for future studies are discussed. 相似文献
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Alvarez I Carrascal M Canals F Muixí L Abián J Jaraquemada D 《Proteomics. Clinical applications》2007,1(3):286-298
HLA class I molecules present peptides on the cell surface to CD8(+) T cells. The repertoire of peptides that associate to class I molecules represents the cellular proteome. Therefore, cells expressing different proteomes could generate different class I-associated peptide repertoires. A large number of peptides have been sequenced from HLA class I alleles, mostly from lymphoid cells. On the other hand, T cell immunotherapy is a goal in the fight against cancer, but the identification of T cell epitopes is a laborious task. Proteomic techniques allow the definition of putative T cell epitopes by the identification of HLA natural ligands in tumor cells. In this study, we have compared the HLA class I-associated peptide repertoire from the hepatocellular carcinoma (HCC) cell line SK-Hep-1 with that previously described from lymphoid cells. The analysis of the peptide pool confirmed that, as expected, the peptides from SK-Hep-1 derive from proteins localized in the same compartments as in lymphoid cells. Within this pool, we have identified 12 HLA class I peptides derived from HCC-related proteins. This confirms that tumor cell lines could be a good source of tumor associated antigens to be used, together with MS, to define putative epitopes for cytotoxic T cells from cancer patients. 相似文献
9.
This paper describes a spectral multigrid method for spatially periodic homogeneous and isotropic turbulent flows. The method uses the Navier–Stokes-αβ equations to accelerate convergence toward solutions of the Navier–Stokes equations. The Navier–Stokes-αβ equations are solved on coarse grids at various levels and the Navier–Stokes equations are solved on the “nest grid”. The method uses Crank–Nicolson time-stepping for the viscous terms, explicit time-stepping for the remaining terms, and Richardson iteration to solve linear systems encountered at each time step and on each grid level. To explore the computational efficiency of the method, comparisons are made with results obtained from an analogous spectral multigrid method for the Navier–Stokes equations. These comparisons are based on computing work units and residuals for multigrid cycles. Most importantly, we examine how choosing different values of the length scales α and β entering the Navier–Stokes-αβ equations influence the efficiency and accuracy of these multigrid schemes. 相似文献
10.
Antonio Ciampi Johanne Thiffault Uri Sagman 《Computer methods and programs in biomedicine》1989,30(4):283-296
The RECPAM methodology previously presented in part I (A. Ciampi et al., Comput. Methods Programs Biomed. 26 (1988) 239–256) is applied to the analysis of survival data on small cell carcinoma of the lung (SCCL). It is shown how RECPAM can help answer the following questions which occur frequently in the analysis of clinical data: Is it possible to find a classification of patients with a certain disease into distinct prognostic groups? Given a covariate of special interest, does it have an independent prognostic significance even after confounding is taken into account? Does the prognostic significance of a covariate of special interest vary across patient subgroups? For the SCCL data, a prognostic classification is obtained and the tumor marker
is treated as a variable of special interest. Many features of RECPAM are illustrated, including, among others, Forward and Backward (Pruning) Stopping Rules, treatment of missing data, and use of several dissimilarity measures. 相似文献