Verification of radio frequency heating uniformity and Sitophilus oryzae control in rough,brown, and milled rice

Radio frequency (RF) heating is considered to be an alternative physical method for disinfesting postharvest agricultural products to replace chemical fumigation due to its inherent dangers on human health and environment. A pilot-scale, 27.12 MHz, 6 kW RF system was used to study the RF heating uniformity and validate the developed RF treatment protocols for disinfesting rough, brown, and milled rice. The results showed that the optimum RF heating uniformity in rough and brown rice was obtained by an appropriate electrode gap of 11 cm with a forced hot air heating at 50 °C, movement of the conveyor with a speed of 12.4 m/h, two mixings, and holding at 50 °C hot air for 5 min. Mortality of adult rice weevils, Sitophilus oryzae (L.), increased with increasing heating temperature and holding time, and reached 100% while RF heating at 50 °C holding in hot air for at least 6 min. There were no significant differences in quality parameters (moisture, protein, water activity, starch, free fatty acid, ash, and color) between RF treatments and untreated controls during storage (P > 0.05). The developed non-chemical alternative RF technology may hold potential for disinfesting rough, brown, and milled rice required by the growing organic market.     

Developing radio frequency technology for postharvest insect control in milled rice

Since methyl bromide fumigation has an adverse effect on human health and environment, it is urgently needed for developing a non-chemical method to replace chemical fumigation for disinfesting milled rice. The purpose of this research was to study possible applications of radio frequency (RF) energy for disinfesting milled rice without affecting product quality. A pilot-scale, 27.12 MHz, 6 kW RF system was used to study RF heating uniformity and develop a treatment protocol for achieving 100% insect mortality and finally evaluating quality attributes in RF treated milled rice during storage. The results showed that the heating time needed only 4.3 min to heat the 3.9 kg milled rice from 25 °C to 50 °C using RF energy, but 480 min for milled rice to reach 48 °C using hot air at 50 °C. After comparing three selected electrode gaps, an appropriate gap of 11 cm was obtained to achieve the heating rate of 5.8 °C/min for further heating uniformity tests. An RF treatment protocol was finally developed to combine 1.0 kW RF power with a forced hot air heating at 50 °C, movement of the conveyor with the speed of 12.4 m/h, two mixings, and holding at 50 °C hot air for 5 min, followed by forced room air cooling through single-layer (2 cm thick) samples. There were no significant differences in quality parameters (moisture, protein, fat, starch, hardness, and color) between RF treatments and untreated controls during storage (P > 0.05). Therefore, RF treatments may provide a practical, effective and environmentally friendly method for disinfesting milled rice.     

Investigation of the Feasibility of Radio Frequency Energy for Controlling Insects in Milled Rice

Many studies showed that radio frequency (RF) holds great potential to control insects in grains, while a few more technical issues need to be addressed to further make this technology ready for industrial application. Therefore, the effect of RF heating rate on rice fissure ratio and broken rate, RF heating lethality on immature rice weevils, and RF disinfestation treatment on cooking and eating quality of milled rice was investigated in this study. Results indicated that RF heating rate had no significant influence on rice broken rate, while fast RF heating rate (>7.2 °C/min) had adverse effects on rice fissure ratio, and mild RF heating rate (<5.8 °C/min) had no significant influence on fissure ratio of milled rice. RF treatment (50 °C, 5 min) could completely control immature rice weevils in rough, brown, and milled rice. Even though RF disinfestation treatment (50 °C, 5 min) influenced the cooking quality (water absorption, adhesive strength) of milled rice, it had no significant effect on overall sensory quality. This study provided valuable information for considering the application of RF energy in milled rice process to control insects.     

Improving radio frequency heating uniformity in milled rice with different packaging shapes by changing temperature of forced air

Radio frequency (RF) treatment is a promising physical method for disinfesting and pasteurizing foods but the non-uniform RF heating is still a major obstacle for industrial applications. In this study, a three-dimensional model was established with COMSOL software to simulate temperature distributions in milled rice with different packaging shapes and experiments using a 6 kW, 27.12 MHz RF system were carried out to evaluate the improvement of heating uniform of sample by changing temperature of forced air. The established model was validated by temperature-time histories at three points in the sample and temperature distributions at the top and bottom surfaces. The results showed that the cold spot of the regular shape sample was found at the part with small thickness, and the RF heating uniformity was improved by increasing temperature of forced hot air. When the temperature of forced hot air increased from 40 to 60 °C, all the sample temperatures were above 50 °C, which might meet the required temperature of disinfestation, and the heating uniformity index decreased from 0.113 to 0.099. For the cuboid shape sample, the corner/edge effect was observed and the heating uniformity index firstly decreased and then increased with reduced temperature of forced cold air. When the temperature of forced air decreased to 10 °C, the best heating uniformity of the cuboid shape sample was obtained. The results of this research can help to select packaging shape and improve the heating uniformity of samples for RF treatment in large-scale applications.     

Radio frequency heating as a disinfestation method against Corcyra cephalonica and its effect on properties of milled rice

Radio frequency (RF) heating has the potential to be developed as an alternative non-chemical disinfestation method. In contrast to microwave (MW), RF exhibited higher penetration depth, which helps RF to be a useful technique in disinfesting packaging foods. A 3 kW, 27.12 MHz RF system was used to validate the practical of radio frequency technology for rice moth (Corcyra cephalonica) control in milled rice. Rice samples were placed in the polystyrene bag and moved at a speed of 0.8 m/min, and heated in the RF system with intermittent mixing. Four electrode gaps and five sample thicknesses were chosen to confirm the optimal conditions of RF treatment. The results showed that the sample thickness of 15 mm and electrode gap of 40 mm could provide the optimum heating rate for rice. Mortality of each stage (adult, larva, egg) of C. cephalonica increased with increasing heating temperature and reached 100% while RF heated 180 s (45.8 °C), 300 s (56.9 °C), and 420 s (70 °C), respectively. No C. cephalonica was determined in the samples during 45 days storage incubation period at RF treatment to 70 °C. There were no significant differences between control and RF treated samples in quality parameters (moisture, protein, fat, gelatinization, and sensory attributes). Therefore, RF treatment may provide a practical and effective method for disinfesting milled rice without affecting product quality.     

Storage stability of pistachios as influenced by radio frequency treatments for postharvest disinfestations

There has been an increased interest in developing alternative physical methods for disinfesting postharvest nuts under growing international pressures to replace chemical fumigation due to its adverse effects on human health and environment. The present research explored the possibility of using radio frequency (RF) heating as a non-chemical treatment for disinfestations of pistachios. A pilot-scale, 27 MHz, 6 kW RF unit was used to study RF heating uniformity, develop a treatment protocol, and evaluate quality attributes and storage stability in treated samples. Only 5.6 and 5.5 min were needed to raise the centre temperature of 1.8 kg in-shell and 2.0 kg shelled pistachios to reach 55 °C using RF energy, respectively, compared to about 82 and 117 min when using hot air heating at 55 °C. RF heating uniformity in both types of pistachios was improved by adding forced hot air, sample movements on the conveyor and a single mixing in the middle of the treatment time. The final average temperatures on the surface and in the interior of both types of pistachio kernels exceeded 52 °C, following a holding step at 55 °C for 2 min using hot air. This provided a conservative and 100% mortality of fifth-instar Indianmeal moth (Plodia interpunctella [Hübner]). RF treated samples were not significantly different from control samples in weight loss, peroxide values, fatty acid values, fatty acid composition and kernel colour. RF treatments can, therefore, potentially provide an effective and rapid protocol against stored product pests in pistachios as an alternative to chemical fumigation.Industrial relevanceA pilot-scale 6 kW RF system with conveyor belt was used to determine the heating uniformity and quality changes of pistachios. For a combination with hot air surface heating and mixing, an effective and continuous RF disinfestation method could be developed for pistachios. The RF heating technology has been successfully demonstrated for disinfesting walnuts in California, USA. We tried to expand the industrial applications of RF heating for disinfesting pistachios, to replace the chemical fumigation. This research may provide potential industrial applications of RF treatments for disinfestations based on fast and uniform heating.     

Validation of radio frequency treatments as alternative non-chemical methods for disinfesting chestnuts

Chemical fumigation has been widely used to control insects in postharvest chestnuts but is inherent dangers when using fumigants. The purpose of this study was to validate application of radio frequency (RF) treatments for disinfesting chestnuts as an alternative to chemical fumigation. A practical process protocol was developed to control insect pests in chestnuts using a 27.12 MHz free-running oscillator RF system. Fifth-instar yellow peach moth, Conogethes punctiferalis, more heat tolerant than chestnut weevil, Curculio elephas, under three temperature and time combinations using a heating block system, was selected as the targeted insect to validate the RF treatment protocol. Mortality of fifth-instar C. punctiferalis increased with increasing holding time at 55 °C using RF heating and reached 100% while holding in hot air for at least 5 min. Furthermore, there was no significant quality difference in color, fat, firmness, moisture content, protein, and soluble sugar content of chestnuts observed between RF treatments and controls. RF treatment methods hold potential to scale up for industrial applications of disinfesting chestnuts.     

Application of radio frequency pasteurization to corn (Zea mays L.): Heating uniformity improvement and quality stability evaluation

Mildews caused grain losses and serious outbreaks have been becoming increasing concerns in domestic and international corn processing industry. This study intended to explore the possibility of using radio frequency (RF) heating as an effective treatment to eliminate the mold contamination and reduce the damage to corn quality. A pilot-scale, 27 MHz, 6 kW RF unit was used to study the heating uniformity in corn samples with five moisture contents (MC) and using three plastic material containers, and develop a treatment protocol for a corn sample with the MC of 15.0% w.b. and evaluate quality attributes and storage stability of treated samples. The results showed that only 7.5 min was needed to raise the central temperature of 3.0 kg corn samples from 25 °C to 70 °C using the RF energy, but 749 min for samples to reach 68.6 °C using hot air at 70 °C. The RF heating uniformity was improved by adding forced hot air, moving samples on the conveyor, and mixing during the treatment. An effective RF treatment protocol was finally developed to combine 0.8 kW RF power with a forced hot air at 70 °C, conveyor movement at 6.6 m/h, two mixings, and holding at 70 °C hot air for 14 min, followed by forced room air cooling through thin-layer (2 cm) samples. Corn quality was not affected by RF treatments since quality parameters of RF treated samples were better than or similar to those of untreated controls after the accelerated shelf life test. RF treatments may hold great potential as a pasteurization method to control molds in corns without causing a substantial loss of product quality.     

Development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) on rice milling components and by-products: Effects of diet and temperature

Development of Tribolium castaneum (Herbst), the red flour beetle, was assessed on different rice components and their various by-products (i.e., diets) commonly found in rice mills, in two separate experiments. In the first experiment, eggs did not develop through to the adult stage on rough rice hulls, paddy rice dust, and milled rice dust, while eggs developed to the adult stage to some extent on rice flour, milled whole kernels, brown rice, milled broken kernels, and bran. For the diets where development occurred, the lowest percentage was on brown rice, and adults that emerged on brown rice had smaller elytra compared to adults emerging on the other diets. In the second experiment, 1–2-day old neonates were exposed on the diets listed above and held at 22, 27, 32, and 37 °C. At all temperatures, development to the pupal and adult stages was slowest on rice flour. At 22 °C, development to those stages took about twice as long compared to development at 27 °C. As temperature increased developmental times were reduced. Even though neonates developed slowest on rice flour adult emergence rates were not affected. Predictive models were used to estimate potential population development on the diets. At 22 and 27 °C, adult size as measured by elytra length was greatest when they developed on bran, while body weight was generally lowest for adults that developed on brown rice. Results show that T. castaneum can utilize rice components and by-products produced during the rice milling process, although not all components or by-products were optimal for development, and emphasis should be placed on cleaning and sanitation to remove food sources to limit infestations. Mill managers can use these results to show the importance of sanitation, and potentially improve overall pest management programs inside the mill.     

Effect of radio frequency heating on the mortality of Rhizopertha dominica (F.) and its impact on grain quality

The use of radio frequency (RF) heating to reach temperatures lethal to stored product pests has significant potential. We examined the lethal effects of RF on the stored grain pest, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), under four different RFs, three types of grain, and different moisture contents of rice. The quality of two rice cultivars, after RF heating, was also tested. When heated to 58 °C by RF, R. dominica adult mortality reached 100%. The mortality of R. dominica adults reached 100%. The fastest mortality rate occurred at 50–52 °C. With an increase in RF, the time needed to attain a lethal temperature decreased. The heating time to reach 100% mortality differed among the three types of grain; for example, the heating time of wheat was faster than corn or rice. Kernel size and chemical composition was the main factor influencing the RF lethal rate in the different types of grain under the same experimental conditions. There was a significant linear relationship between moisture content (m.c.) and heating time (Y = −38.05 X + 15.501, R = 0.9803) in rice. RF heating of rice to 58 °C did not significantly affect milling quality, storage quality, or seed quality. Thus, heating time, types of grain, and the m.c. of the grains are three important factors that should be considered in RF heating to control stored product pests. These results indicate that RF heating could be an effective physical method for killing stored grain pests.     

Developing radio frequency heating treatments to control insects in mung beans

Radio frequency (RF) treatments are often used for disinfestations while maintaining the quality of postharvest agricultural products. The purpose of this research was to determine the efficacy of RF treatments for disinfesting mung beans. A pilot-scale, 27 MHz, 6 kW RF unit was used to study the heating uniformity of 6.0 kg mung beans in three different packaging methods heated from 25 °C to 54 °C, and the quality of RF treated mung beans was evaluated. The results showed that the best treatment was achieved by using an eight-layer polypropylene packaging method, an electrode gap of 12.5 cm for heating 5 min, 54 °C surface forced air heating, and 6 min 54 °C forced air holding due to the low heating uniformity index. Then a single layer of forced room air was used for cooling to avoid quality degradation. There was no significant difference in the moisture content, water activity, protein, starch, germination rate, and color values of mung beans before and after RF treatments. Therefore, RF treatments may hold the potential for the effective disinfestations of mung beans.     

Hot air-assisted radio frequency heating effects on wheat and corn seeds: Quality change and fungi inhibition

Hot air-assisted radio frequency (RF) heating has been proposed and studied as an alternative disinfestation method for grain seeds. In this study, hot air-assisted RF heating effects on fungi inhibition and its influence on physiological (seed vigor, germination rate) and biochemical (color, enzyme activity) qualities of wheat and corn seeds were further investigated. Results showed that high intensity hot air-assisted RF treatment (70 °C, 10 min) reduced seed vigor and germination rate for both wheat and corn seeds, but relatively mild intensity treatment (65 °C, 10 min) showed no significant influence on color and germination rate, and even enhanced enzyme activity (Superoxide dismutase-SOD, catalase-CAT and peroxidase-POD). Moreover, hot air-assisted RF treatment (65 °C, 10 min) reduced Aspergillus flavus by 2 and 3 log-scale (CUF/g) in wheat and corn seeds with the moisture content of 12.0%, and 3 and 4 log-scale for wheat and corn seeds when moisture content increased to 15.0%, respectively. These results indicated higher moisture content (a_w) of grain seeds would benefit fungi inhibition for hot air-assisted RF treatment, and with proper control of treatment conditions, hot air-assisted RF heating has the potential to inhibit fungi and maintain physiological and biochemical quality of grain seeds.     

Infrared Heating as a Disinfestation Method Against <Emphasis Type="Italic">Sitophilus oryzae</Emphasis> and Its Effect on Textural and Cooking Properties of Milled Rice

Infrared (IR) heating method against rice weevils (Sitophilus oryzae) in an egg stage was investigated. A kinetic model was developed to describe insect mortality in a temperature range of 40–60 °C. Effects of IR heating temperature (50–60 °C) and exposure time (1–3 min) on insect mortality and quality attributes of the treated rice were evaluated. The optimized condition obtained by means of the response surface method was used to analyze rice quality before and after IR treatment with storage. The results showed that the 0.5th-order thermal death kinetic equation was the most suitable model, and the S. oryzae eggs had less heat tolerance than the adults and some other species. Mortality achieved 100 % after 2 min for all temperatures. Both IR heating parameters significantly affected the treated milled rice qualities. The minimal changes in rice quality before and after storage could be obtained using optimized temperature and exposure time of 53.6 °C and 1.2 min, respectively.     

Impacts of Storicide II on internal feeders of Brown rice

Protectants applied to grain can reduce damage caused by insect feeding during storage. Although these protectants are effective against many external feeders, they may also reduce damage caused by internal feeders as they often interact with the exterior surface of the grain during their larval or adult stages to feed or oviposit. For this study, we investigated impacts of Storicide® II applied to brown rice on three different internal feeders: Rhyzopertha dominica (Fauvel), Sitophilus oryzae (L.), and Sitotroga cerealella (Olivier). We also investigated the effects of this protectant at three different temperatures and when it was combined with different percentages of untreated brown rice. Time-series clustering was also performed to determine whether treatments caused disruptions to the timing of progeny emergence. Overall, R. dominica was the most susceptible as mortality and knockdown were observed in mixtures containing 10% treated brown rice. In contrast, S. cerealella was the least susceptible as mixtures containing at least 50–75% treated brown rice were required to reduce progeny production. However, lowering the temperature to 22 °C did reduce the amount of treated brown rice required to reduce progeny emergence and also reduced the number of progeny that emerged synchronously, which would likely reduce mating and reduce population levels over time. Similar effects on progeny were observed for S. oryzae and R. dominica. Overall, these findings suggest that Storicide II can reduce population levels of internal feeders and that combining this protectant with cooler temperatures can provide additional protection.     

The use of modified atmospheres to control Sitophilus zeamais and Sitophilus oryzae on stored rice in Portugal

This study reports the efficacy of using CO₂ against Sitophilus zeamais and Sitophilus oryzae as an alternative treatment to fumigation for rice stored in a rice mill in Portugal. The trials were conducted in a silo containing 40 tonnes of polished rice and in four hermetic big bags of 1 tonne capacity; two with paddy and two with polished rice. The composition of the atmosphere ranged from 90 to 95% CO₂ and 0.7–2.1% O₂. Three trials were carried out at different temperatures and treatment times; stored rice in the silo at 29.6 ± 0.1 °C for 26 days (first trial), at 34.1 ± 0.2 °C for 10 days (second trial), and in big bags at 22 °C for 26 days (third trial).To evaluate the efficacy of each treatment, metal cages with 16 g of infested rice where placed at bottom, middle, top and surface of the polished rice in the silo. Four replications of each type of infested rice containing one-week-old S. zeamais adults, or eggs of S. zeamais or S. oryzae, were incubated in the laboratory, at the same temperature as in the silo, to serve as a control.In all modified atmosphere treatments adults of S. zeamais, and eggs of both S. oryzae and S. zeamais, showed mortality close to 100% and no F1 emergence was recorded in any treatment sample. This was the first time that a Portuguese rice mill used modified atmospheres.     

Characterization of endogenous enzymes of milled rice and its application to rice cooking

The relationship between the activities of endogenous enzymes in milled rice and accumulation of chemical components in the rice grains during cooking was investigated. Maltose and soluble starch were optimally hydrolyzed around 60 °C by both crude extracts and purified α-glucosidases of milled rice. Gelatinization onset temperature of rice flour was determined to be 63 °C with DSC analysis under usual cooking condition. Hydrolytic activities on carbohydrates and proteolytic activities of milled rice were enhanced at pH 5 compared to pH 7 at the range of 4–80 °C. When rice was cooked at pH 5, glucose and amino acids highly accumulated with soaking for 16 h before heating. We propose a new method of cooking with prolonged soaking at acidic pH, which is effective for increasing the amounts of chemical components in cooked rice.     

Characterisation of Brown and Milled Yellow Rice and Development of an Expanded Snack

Brown rice and yellow milled rice were characterised in relation to milling properties, cooking, processing quality and microbial testing, and utilised to develop an expanded snack. The extrusion process was done in a Mapimpianti single screw cooker-extruder. A flour sample feed rate of 70 g (dry matter) min⁻¹ was maintained by varying the force-feeder speed. A screw speed of 150 rev min⁻¹ and a die with 20 die-nozzle orifices (2 mm in dia) were used. The die zone was heated at 110°C by electrical resistance. Compressed air was circulate around the barrel to maintain precise control of the temperature. The moisture content of the samples was 120, 150 and 180 g kg⁻¹. The grains were classified as long-thin with an average size of 2·13 mm×6·79 mm. The milling yields obtained in the laboratory with paddy rice were 700 g kg⁻¹ brown rice and 600 g kg⁻¹ milled rice. Brown rice and yellow milled rice had similar amylose contents, 225 and 256 g kg⁻¹, respectively. Gel consistency was soft with low gelatinisation temperature (63–68°C) for both samples. Field fungi such as Helminthosporium oryzae and storage fungi as Aspergillus spp were present in paddy, yellow milled and commercial rice. Helminthosporium oryzae was not present in extruded products. The extruded products showed low density and a high degree of expansion, with the optimum degree of expansion obtained in flours processed with moisture at 150 g kg⁻¹ in both milled and brown rice. The highest values for water solubility index were obtained with flours from milled yellow rice and none of the extruded products showed significant differences on water absorption index. Sensory analyses carried out on the snack products showed them to be acceptable, with the bent acceptance for products made from milled yellow rice processed with 150 g kg⁻¹ moisture.     

Effect of radio frequency (RF) heating on the texture,colour and sensory properties of a comminuted pork meat product

Radio frequency (RF) cooking is a form of dielectric heating in which products are heated by subjecting them to an alternating electromagnetic field between two parallel electrodes. Although similar in some respects to Microwave heating, RF has been proposed to be more suitable for industrial heating of meats because of the greater penetration depths possible with this technology. In this study an RF cooking protocol was developed and its effect on selected quality attributes of pork based white pudding was examined. Whilst cooking of the product in air proved unfeasible due to arcing, use of a polyethylene cell with circulating hot water (80 °C) facilitated successful heating of the product. Application of RF using an optimised cooking protocol (RF power = 450 W, cell volume = 500 ml and continuous circulation) resulted in a mean end-point temperature of 73 °C after 7 min 40 s. Similar mean end-point temperatures in water bath and steam oven heated products were achieved after 29 and 33 min, respectively. A factorial experiment was conducted to assess selected quality attributes of the cooked puddings. Results show that RF heated puddings were not significantly different (P > 0.05) from water bath and steam oven heated products with regard to instrumental colour, instrumental texture (Kramer shear and texture profile analysis) and expressible fluid. Furthermore, results of a sensory similarity test involving 60 panellists indicated that panellists were not able to detect differences between puddings cooked by RF and conventional methods. Overall this suggests that RF heating technology could have potential in pasteurisation of meat products though further work is needed to verify this.     

Effects of layer arrangement on heating uniformity and product quality after hot air assisted radio frequency drying of carrot

The conventional drying technology prolongs the shelf life of carrots but may cause the serious loss of nutrients. The aim of this study was to investigate the effects of intermittently rearranging layers on the heating uniformity, drying characteristics and quality of carrot slices under the hot air assisted radio frequency (RF) heating. The carrot slices were dried for 270 min using hot air (60 °C) assisted RF heating at the electrode gap of 100 mm firstly, and then followed hot air drying to achieve the final moisture level (0.11 kg/kg (d.b.)). The results showed that the selected hot air assisted RF drying protocol for the carrot slices reduced 30% of the duration compared to the single hot air drying. The carrot slices dried by the combined drying method had the highest total carotenoid value (P ≤ 0.05) except for possessing accepted color and rehydration. Therefore, the combined drying method could improve the drying rate and maintain heat sensitive substances in carrot slices.     

Developing treatment protocols for disinfesting pine wood product using radio frequency energy

Numerous non-native insect pests are moved around the world via international trade and have caused major forestry losses, especially for pine trees. The purpose of this research was to study a potential application of radio frequency (RF) heating for disinfesting pine wood products to reduce forestry damage caused by exotic pests. A pilot-scale 6 kW, 27.12 MHz RF system was used to develop a treatment protocol for disinfesting pine wood products based on the heating uniformity studies. The results showed that the heating time needed was only 6.7 min to heat the wood blocks (250?×?400?×?60 mm³) with moisture content of 13.3% d.b. from 25 to 60?°C using RF energy, but 320 min to reach 56.2?°C using hot air at 60?°C with 1.6 m/s. Based on the heating uniformity study, the best condition occurred when the lower wood moisture content was used and the wood width approached the RF electrode one. The RF treatment protocol was finally developed to combine RF energy with forced hot air at 60?°C, movement of conveyor at 7.4 m/h and holding at 60?°C hot air for 1 min. Wood quality was not affected significantly by the RF treatments because quality parameters of treated wood samples were similar to those of controls. The RF treatment may provide a rapid, effective and environmentally friendly method to disinfest wood products.