共查询到18条相似文献,搜索用时 347 毫秒
1.
2.
对虎尾草药材地上部分用70%乙醇提取后,以大孔吸附树脂、聚酰胺和硅胶等分离材料进行分离和纯化,从中分得10个黄酮类化合物,利用氢谱、碳谱、质谱和颜色反应等手段对其结构进行确认,10个化合物依次为槲皮素(Ⅰ)、山奈酚(Ⅱ)、异鼠李素(Ⅲ)、槲皮素-3-O-β-葡萄糖苷(异槲皮苷)(Ⅳ)、槲皮素-3-O-α-L-鼠李糖苷(槲皮苷)(Ⅴ)、山奈酚-3-O-β-D-葡萄糖苷(Ⅵ)、山奈酚-3-O-β-D-半乳糖苷(Ⅶ)、金丝桃苷(Ⅷ)、异鼠李素-3-O-β-D-芸香糖苷(Ⅸ)和芦丁(Ⅹ),其中化合物Ⅴ、Ⅵ为首次从该植物中分离得到,化合物Ⅲ、Ⅸ为首次从该属植物中分离得到。 相似文献
3.
4.
沙棘叶黄酮糖苷生物转化黄酮苷元研究 总被引:8,自引:0,他引:8
沙棘叶中的黄酮类化合物主要以糖苷形式存在,现采用酶法水解糖苷中的糖基使其变成苷元,提高抗氧化活性.沙棘黄酮糖苷经过α-鼠李糖苷酶(1 IU)、β-葡萄糖苷酶(2 IU)、木聚糖酶(1.5 IU)、纤维素酶(15 IU)、果胶酶(0.5 IU)、α-淀粉酶(2 IU)组成的复合酶预处理后,黄酮糖苷转化率仅为0.23%,再用柚苷酶水解可获得高含量的黄酮苷元,转化率达85%以上,其中槲皮素占总黄酮苷元的23.31%,异鼠李素占35.11%,山奈酚占9.95%.沙棘黄酮苷元不易溶于水而溶于乙醇. 相似文献
5.
6.
该文对大孔吸附树脂纯化黄花蒿黄酮的工艺条件进行了研究与优化,并对纯化后的黄酮进行了抗氧化活性的研究。比较了AB-8,DM-101,DA-201,D-101及SD-401对黄花蒿中黄酮类物质的吸附及解吸附性能,结果显示,D-101的综合效果最佳。通过D-101大孔吸附树脂动态吸附解吸实验,获得较佳的纯化工艺:上样液黄酮质量浓度为1.55 g/L,流速为2 mL/min,上样100 mL后,用蒸馏水洗脱至溶液无色,再用250 mL体积分数70%乙醇以1 mL/min洗脱。在该条件下,黄酮质量分数从20.10%提高到80.32%,洗脱率达91.08%,黄酮回收率为68.25%。黄花蒿黄酮对油脂有明显的抗氧化性作用;黄花蒿黄酮对植物油的抗氧化能力强于柠檬酸和抗坏血酸,对动物油脂的抗氧化能力稍弱于抗坏血酸而略强于柠檬酸。 相似文献
7.
对国内几种大孔吸附树脂的丹酚酸B吸附性能进行了实验筛选。结果表明。YWD06树脂有较好的吸附和洗脱能力;适宜吸附条件:pH控制在4~5、流速为2BV/h;以70%乙醇为洗脱剂,pH=5、流速为3BV/h时,洗脱效果好。 相似文献
8.
9.
10.
对国内几种大孔吸附树脂的丹酚酸B吸附性能进行了实验筛选。结果表明。YWD06树脂有较好的吸附和洗脱能力;适宜吸附条件:pH值控制在4-5、流速为2BV/h;以70%乙醇为洗脱剂,pH值=5、流速为3BV/h时,洗脱效果好。 相似文献
11.
《分离科学与技术》2012,47(15):3717-3734
Abstract In the present study, the performance and adsorption characteristics of five macroporous resins for the separation of glabridin from Glycyrrhiza glabra L. have been evaluated. The adsorption and desorption properties of glabridin on macroporous resins including HPD100, HPD300, HPD800, NKA and H103 were compared. HPD100 resin offered the best adsorption and desorption capacities based on the research results. Both Langmuir and Freundlich isotherms were used to describe the interactions between the solutes and resins at different initial concentrations. Dynamic adsorption and desorption experiments on HPD100 resin packed column were conducted to optimize the separation process of glabridin from licorice extracts. After the treatment with stepwise elution on HPD100 resin, the content of glabridin in the product increased from 0.21% to 32.2% which is 153-fold higher than it in G. glabra L. roots and the recovery yield was 79.7%. The results indicated the good ability of HPD100 resin for separation glabridin and the study may provide scientific references for the large-scale glabridin production from G. glabra L. or other plants extracts. 相似文献
12.
13.
14.
F Yang L Yang W Wang Y Liu C Zhao Y Zu 《International journal of molecular sciences》2012,13(7):8970-8986
In order to screen a suitable resin for the preparative simultaneous separation and purification of syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus, the adsorption and desorption properties of 17 widely used commercial macroporous resins were evaluated. According to our results, HPD100C, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for syringin, eleutheroside E and isofraxidin than other resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions were as follows: for adsorption, processing volume: 24 BV, flow rate: 2 BV/h; for desorption, ethanol-water solution: 60:40 (v/v), eluent volume: 4 BV, flow rate: 3 BV/h. Under the above conditions, the contents of syringin, eleutheroside E and isofraxidin increased 174-fold, 20-fold and 5-fold and their recoveries were 80.93%, 93.97% and 93.79%, respectively. 相似文献
15.
微波法合成硫脲树脂及其吸附性能研究 总被引:5,自引:0,他引:5
研究了硫脲、甲醛、对苯二酚的聚合,比较了微波辐射和常规加热对树脂产率的影响,考察了不同条件下树脂对金属离子的吸附情况。用核磁共振和红外光谱对树脂可能含有的官能团进行表征。结果表明:微波辐射与常规加热相比具有反应时间短、产率高的优点。酸性条件下树脂对Ag 的最高吸附量可达2.2mmolg-1,而相同条件下对Ni2 、Cd2 、Co2 等过渡金属离子基本不吸附,体现了该树脂在对金属离子的选择分离方面有较高的应用价值。利用含8%硫脲的1molL-1HNO3溶液洗脱经吸附过的树脂,洗脱率可达95%以上,再生的树脂吸附量仍可达到前一次的98%。 相似文献
16.
大孔树脂对贯叶连翘中金丝桃素的静态吸附 总被引:1,自引:0,他引:1
考察了18种不同类型的大孔树脂对贯叶连翘中金丝桃素的静态吸附和解吸效果,并对3种效果较好的树脂进行了吸附动力学研究;比较了上样液pH对吸附效果的影响;探讨了NKA-9树脂在25℃等温吸附过程,并应用Langmuir方程进行了拟合。结果表明,弱极性树脂NKA-9对金丝桃素吸附解吸作用最好,到达吸附平衡时间为1h左右,吸附量可达12.45mg/g干树脂。上样液最佳pH=4,上样液pH对树脂的吸附有很大影响。NKA-9树脂对金丝桃素的吸附为单分子吸附,符合Langmuir吸附理论。 相似文献
17.
《分离科学与技术》2012,47(16):2601-2611
ABSTRACTIn this study, Monascus pigments were separated from the extractive fermentation broth by the combination of reverse micellar extraction and macroporous resins (MACR) adsorption with the alkalizing S-8 MACR (S-8 ALR). A 95.07% pigment adsorption was achieved together with a relative low Triton X-100 co-adsorption that was only 2.53%. The liquid intraparticle diffusion and film diffusion limit the pigment adsorption rate. A 79.48–84.03% of the pigment could be eluted from the resin using the methanol solution containing a 0.5% (w/v) hydrochloric acid. The S-8 ALR could be recovered for cycle use without a deficit of the adsorption or desorption efficiency.Abbreviations: ACR—Acidified resin; ALR—Alkalizing resin; HPLC—High performance liquid chromatography; PSM—pigment- surfactant micelle; TEM—Transmission electron microscopy. 相似文献
18.
ABSTRACTIn this study, the combination of macroporous adsorbent resins (MARs) and thin-film evaporation coupling distillation (TFECD) was studied systematically, with aim to obtain high value-added squalene from soybean oil deodorizer distillate (SODD). Five types of resins (X-5, D-101, D4020, DM301, and AB-8) were first used to evaluate the adsorption/desorption properties of squalene. D101 resin exhibited higher adsorption/desorption capacities and desorption ratios for squalene based on static adsorption results among the tested resins. We further investigated the adsorption kinetics, isotherms, and thermodynamics with D101 resin as adsorbent. The adsorption of squalene on D101 was best fitted to pseudo-second-order kinetic model and the Langmuir equation. The dynamic adsorption and desorption indicated that similar results were observed in the static adsorption test. The purity of squalene was increased from 7.5 to 82.5% with the recovery up to 88.5% after separation on D101 column. The resin-refined sample was directly subjected to TFECD purification. Under optimized process parameters, the final product with purity of 98.5% and recovery yield of 76.5% was confirmed by high-performance liquid chromatography (HPLC) analysis. The present study provided an effective method for large-scale production of high purity squalene. 相似文献