共查询到19条相似文献,搜索用时 62 毫秒
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采集了3个孝感凤窝米酒曲样品,使用Miseq高通量测序技术对其真菌微生物多样性进行了评价。在属水平上,相对含量>0.5%的属分别为隶属于毛霉亚门(Mucoromycotina)的淀粉霉(Amylomyces)、小克银汉霉属(Cunninghamella)和毛霉属(Mucor)及隶属于子囊菌门(Ascomycota)的复膜孢酵母属(Saccharomycopsis)、曲霉属(Aspergillus)、念珠菌(Candida)、拟威尔酵母(Cyberlindnera)和接合酵母(Zygosaccharomyces)。在分类操作单元(OUT)水平上,发现26个核心OTU,包含的序列数占所有质控后合格序列数的94.32%。由此可见,孝感凤窝酒曲中的真菌微生物主要由隶属于毛霉亚门和子囊菌门的若干个真菌属构成,且米酒曲样品共有大量的核心真菌菌群。 相似文献
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白酒酒曲中9株酵母菌的分离与特性研究 总被引:1,自引:0,他引:1
从本地白酒酒曲中成功分离出9株酵母菌,依次命名为Y-1~Y-9。比较了这9个菌株的生理生化特性、耐高温能力和耐酒精能力等特性。结果表明:分离的9株酵母菌上述特性差别较大,其中,Y-4各方面均表现突出,该菌株能利用木糖在内的多种糖源,在45℃下生长良好,能耐14%的酒精度。对橘子汁为原料进行的发酵实验表明,上述菌株均能以橘子汁为原料生产酒精,其中,Y-4发酵能力最强,发酵84h后,CO2失重为12.913g。综上所述,Y-4是一株极具潜力的燃料乙醇生产工程菌株。 相似文献
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为研究番茄自然发酵液中的酵母菌及其特性,对发酵30 d番茄发酵液中的酵母菌分离纯化,通过菌落形态、镜检特征和26S rDNA序列对分离的菌株进行鉴定,并对该菌的生长特性和耐受性进行评价。结果表明,从番茄发酵液中分离出TY-1、TY-2和TY-3 3株酵母菌均为毕赤酵母属(Pichia)中的库德毕赤酵母种(P.kudriavzevii);其中TY-1和TY-2亲缘关系较近,且与P.kudriavzevii strain SLDY-035菌株的同源性为99%,故选取TY-1进行后续实验;TY-3与P.kudriavzevii small subunit ribosomal菌株的同源性为100%。TY-1和TY-3的最佳生长温度为29℃、pH5.5,对10%~30%葡萄糖和6%~9%酒精具有一定的耐受能力。结果表明,从番茄发酵液中分离得到的酵母菌株具有良好的生长性能,可为果蔬发酵剂的开发奠定基础。 相似文献
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目的:探明孝感米酒中乳酸菌的种类及其对黄酒品质的影响。方法:采用传统可培养方法使用MRS培养基、厌氧工作站,对孝感米酒中乳酸菌进行分离,并接入黄酒对黄酒发酵过程进行干预,进而通过分析黄酒有机酸与滋味等理化特征来评价乳酸菌的发酵特性。结果:从孝感米酒中分离到9株乳酸菌,基于16S rDNA基因对乳酸菌分离株进行了系统发育分析,结果显示它们属于魏斯氏菌属(Weissella)、片球菌属(Pediococcus),种水平上分为戊糖片球菌(Pediococcus pentosaceus)、融合魏斯氏菌(Weissella confusa)、食窦魏斯氏菌(Weissell acibaria)。将这9株菌应用于黄酒发酵制作,在菌株融合魏斯氏菌MJ3-1与MJ4-1、戊糖片球菌MJ6-1、食窦魏斯氏菌MJ8-1干预下,黄酒中乳酸含量显著增加(p<0.05),苹果酸含量显著减少(p<0.05);同时黄酒的滋味中咸味和丰度有显著增加(p<0.05)。总体上来说,乳酸菌对黄酒滋味的影响由酸味、鲜味与丰度引起。结论:融合魏斯氏菌MJ4-1能增加黄酒乳酸,并降低苹果酸含量作用最强,适用于黄酒复合型发酵剂的研发。 相似文献
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蜂蜜米曲发酵酒工艺的研究 总被引:1,自引:0,他引:1
将蜂蜜稀释,加入制备好的米曲汁30%和0.1%硫酸铵,灭菌后接入酵母,于25℃发酵15~25d,经过滤、澄清、灭菌后勾兑,再陈酿3个月即得到营养丰富、风味独特的蜂蜜米曲发酵酒。(丹妮) 相似文献
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Antonio Bevilacqua Luciano Beneduce Milena Sinigaglia Maria Rosaria Corbo 《Journal of food science》2013,78(5):M742-M751
Ninety‐nine yeasts were isolated from Bella di Cerignola table olives; first, the strains were studied in relation to their ability to produce biogenic amines in a laboratory medium and 49 strains were positive to this assay and cut off from the research. The remaining 50 strains were characterized for their enzymatic traits (β‐glucosidase, catalase, pectolytic, xylanolytic, and lipolytic activities) and for their ability to grow at different temperatures, pHs, with salt or lactic/acetic acids added. Data were used for the evaluation of growth index and submitted to cluster and principal component analyses to choose the most promising 4 strains. In the final step of the research, the strains were inoculated as a cocktail in a model brine, containing different amounts of salt (4% to 12%) and glucose (0% to 3%), and adjusted to different pHs (4.0 to 9.0). Data analysis through a multiple regression procedure highlighted that salt, glucose, and pH acted in a different way within the storage and NaCl affected yeast growth only for few days, and then glucose and pH played a major role. Practical Application Olive fermentation relies upon a complex microflora, including lactic acid bacteria and yeasts; the selection of suitable strains of yeasts intended as starter cultures, as well as their inoculation in brines, could improve the fermentation. 相似文献