富硒大豆的灵芝菌丝体液态发酵条件优化

以富硒大豆为原料,采用液体发酵技术对富硒大豆的灵芝菌丝体液态发酵条件进行研究。富硒大豆经浸泡、磨浆、过滤、煮沸后接种灵芝菌种发酵,以水解度为指标,通过单因素和正交试验对温度、初始pH值、摇瓶转速及豆浆质量分数等发酵条件进行优化。结果表明：富硒大豆的灵芝菌丝体液态发酵最佳条件为发酵温度28℃、培养基初始pH5.8、摇瓶转速160r/min、豆浆质量分数5%、发酵时间132h。在最佳发酵条件下,大豆蛋白的水解度达27.8%、大豆多肽产量7.0178mg/L、多糖产量1.75mg/L、灵芝酸产量0.66mg/L。     

液体深层发酵条件对银耳菌丝体酸性多糖富集的影响

考察液体发酵培养基中不同碳源、碳源用量以及培养基pH 值对银耳菌丝体富集酸性多糖的影响。结果表明：1% 的蔗糖、pH 值为7.0 的培养基有利于银耳菌丝体酸性多糖的富集。并对所得银耳多糖进行DEAE-sepherosefast flow 柱层析,发现菌丝体与子实体相比,多糖组成发生显著改变。     

发酵型保健酒的研究

介绍百野助寿保健酒的生产过程。灵芝菌种经培养,形成成熟的灵芝菌丝体;选用的木耳、银耳、大枣、枸杞、香菇、金针菇等原料经前处理制成胶体状;再把糯米进行膨化后,加入酒曲进行前发酵。然后将三者进行混合,再次发酵,形成既有米香又有植物香的液体。     

银耳腐竹及其关键生产工艺研究

研究银耳腐竹生产的关键技术。在单因素试验基础上,确定银耳豆浆汁浓度、银耳与大豆质量比与银耳豆浆汁pH值为关键因素,以银耳腐竹产率和韧性为指标,采用L9（34）正交试验优化银耳腐竹生产工艺参数。结果表明银耳腐竹关键生产工艺参数的最佳组合为：银耳与大豆的质量比为5∶100（w·w-1）,银耳豆浆汁浓度为2.2°Bé,银耳豆浆汁pH值为7.5。以此最佳工艺参数组合为条件,并将其他影响因素设定在适宜参数范围,制备银耳腐竹,其产率可达5.11g·（100mL）-1,产品的韧性好,为0.716kg。     

银耳酸豆奶的生产工艺

银耳酸豆奶是以大豆为主要原料,经脱腥除臭处理,提取出蛋白质等营养成分,并添加适量的银耳提取液,接入有利于人体健康的乳酸菌进行发酵而成。银耳酸豆奶外观呈乳白色凝固状,酸甜适口,具有典型的乳酸发酵香味。经测定其蛋白质含量为2.5%     

银耳露

“银耳露”是利用啤酒生产排放的低糖麦芽汁为基本原料,加入银耳芽孢进行深层发酵,佐以名贵中药(如当归、党参等)配     

银耳发酵酸乳的研制

以银耳、乳粉为原料,经过预处理、添加适量稳定剂,进行乳酸发酵研制出具有特殊香气和营养价值的银耳酸乳。通过正交试验确定了银耳汁制备的最佳条件为料水比1：200、浸提温度100℃、浸捉时间90min;银耳汁乳酸发酵的最佳工艺条件为乳粉添加量为5％,乳酸菌接种量为6％,发酵温度为42℃,蔗糖添加量为7％。稳定剂用明胶0．05％效果最好。     

中式干酪——豆酪的研制

以大豆为原料,经浸泡、磨浆、滤浆、煮浆等工序,利用乳酸菌对豆浆进行发酵,使豆浆中的蛋白质凝固,并确定最佳菌株组合和发酵工艺再对豆浆凝块进行压榨、划坯,接种霉菌发酵,并对其进行风味的调整,从而得到色泽美观、质地紧密、咸淡适宜、香味醇厚的产品。     

灵芝菌发酵豆奶的研究

为了提高制备发酵型灵芝菌豆奶的营养保健作用,以豆浆为原料进行灵芝菌发酵。研究了不同豆浆浓度、葡萄糖和蔗糖对灵芝菌发酵的影响,同时,选取对灵芝菌发酵豆奶影响最大的两个因素进行中心组合旋转设计。结果表明：在促进灵芝菌的生长方面添加葡萄糖要优于蔗糖。利用响应面分析对豆浆浓度和葡萄糖的添加量进行优化结果表明：最佳豆浆浓度为100%,葡萄糖添加量为1.657%,灵芝菌发酵的生物量可达最理想值。     

银耳蒲菜保健酸奶的工艺研究

以优质的银耳、蒲菜为主要原料,选用保加利亚乳杆菌和嗜热链球菌以1︰1组成混合发酵剂进行乳酸发酵,制得酸奶。通过正交试验和响应面设计得出最佳发酵条件:接种量4%,发酵时间5 h,发酵温度42℃;最佳工艺配方为:银耳浆14%,蒲菜浆20%,白砂糖6%。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press