乳铁蛋白对卵清白蛋白过敏小鼠外周血中Th1/Th2细胞平衡的影响

利用卵清白蛋白(OVA)建立BALB/C小鼠食物过敏模型,观察乳铁蛋白(lactoferrin,Lf)对该食物过敏机体的免疫调节作用。小鼠分别接受预防性乳铁蛋白处理和胃肠道刺激期间灌胃乳铁蛋白,最后一次处理1h后检测其外周血中IFN-γ、IL-4、TGF-β、IL-6、IL-10、IL-12p40和IgE的变化。结果表明:2mg/(kg.d)(以体质量计)的Lf在胃肠道刺激期间显著提高了IFN-γ和IL-12p40水平而降低了IL-4和IL-6水平,但20mg/(kg.d)的Lf降低了所有这些细胞因子。同时,Lf明显降低了血清中IgE浓度而提高了IFN-γ/IL-4比值,20mg/(kg.d)的Lf对IgE影响较大,2mg/kg的Lf对IFN-γ/IL-4比值影响较大。此外,预防性处理中的Lf均显著降低了IL-4、IL-6、IL-10、IL-12p40、IFN-γ、TGF-β和IgE水平,并且20mg/(kg.d)的Lf效果较好,且该剂量明显提高了IFN-γ/IL-4比值。因此,Lf能够改善OVA诱导的食物过敏小鼠Th1/Th2细胞的平衡,具有重要的免疫调节作用。     

卵转铁蛋白对免疫低下小鼠Th1/Th2细胞平衡的影响

目的:研究卵转铁蛋白(OVT)对免疫低下模型小鼠的免疫平衡恢复作用。方法:将健康小鼠随机分为正常对照组、模型对照组及OVT高剂量组、中剂量组和低剂量组,每组10只。OVT高、中、低剂量组分别以10、1、0.1mg/mL的OVT溶液每天灌胃,灌胃量为20mL/(kg.d)(以体质量计)。模型组对照组和正常对照组灌胃生理盐水20mL/(kg.d)。连续灌胃15d,于第15天灌胃后注射环磷酰胺(正常对照组除外),继续灌胃至第19天(由模型决定)进行检测。测定血清中IFN-γ和IL-4含量、IFN-γ/IL-4比值以及脾脏和胸腺指数。结果:模型对照组小鼠血清IFN-γ的浓度和IFN-γ/IL-4比值与正常对照组相比明显降低(P<0.01);高剂量组小鼠血清IFN-γ的浓度和IFN-γ/IL-4比值显著高于模型对照组(P<0.01),与正常对照组比较,无显著性差异;高剂量组的脾脏指数和胸腺指数与模型对照组相比明显提高(P<0.01)。结论:OVT能修复由环磷酰胺所致免疫低下模型小鼠的体液和细胞免疫失衡,增强免疫低下小鼠的免疫功能。     

A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)

Lycium barbarum has been used as a traditional Chinese medicine (TCM) to nourish liver and kidney, and brighten the eye. The fruits are dried or freshly squeezed for their juice and concentrated for beverages. Among various constituents, a group of polysaccharides (LBP) with a Glycan-O-Ser glycopeptide structure has been most researched and considered to be important for the efficacy of L. barbarum. Studies indicate effects of L. barbarum on aging, neuroprotection, general well-being, fatigue/endurance, metabolism/energy expenditure, glucose control in diabetics, glaucoma, anti-oxidant properties, immunomodulation, anti-tumor activity and cytoprotection. In addition to TCM, L. barbarum can be sold as a dietary supplement or classified as a food based upon the long and safe traditional usage. This review is to provide background and updated information of chemical constituents and efficacies with safety including a new direction for research and current regulatory situation of L. barbarum.