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1.
菠菜提取物抗氧化性研究   总被引:3,自引:0,他引:3       下载免费PDF全文
本文研究了菠菜的抗氧化性,采用不同极性溶剂对菠菜中的抗氧化性物质进行提取,测定不同极性提取物中的总酚、总黄酮含量及DPPH自由基清除率,发现95%乙醇本本效果最好本95%乙醇本本率为4.96%;本本含量为39.60μg/mg样品;本黄酮含量为17.53μg/mg样品;终浓度0.5mg/mL时本DPPH自由基本清除率为62.09%。  相似文献   

2.
镇江香醋抗氧化活性成分来源分析   总被引:15,自引:0,他引:15  
文中研究了镇江香醋生产过程中抗氧化性成分酚类、黄酮类化合物含量及DPPH自由基清除活性的变化,并分析了总酚、总黄酮与DPPH自由基清除活性的相关性。研究表明,在醋酸发酵阶段,镇江香醋总酚含量及总黄酮含量开始明显增加。露底封醅阶段和煎煮过程对镇江香醋DPPH自由基清除作用有较明显的影响。总酚含量与总黄酮含量与DPPH自由基清除活性有较高的相关性,相关系数分别为0 9334和0 935 3。  相似文献   

3.
26种市售食醋抗氧化性的比较   总被引:5,自引:0,他引:5  
测定了不同品种食醋中酚类、黄酮类化合物含量及DPPH自由基清除活性,并分析了总酚、总黄酮含量与DPPH自由基清除活性的相关性。结果显示,不同品种食醋的自由基清除作用和它们含有的酚类及黄酮类成分相关,相关系数分别为0.952 3和0.869 1。总酚含量、总黄酮含量可作为评价食醋抗氧化性的指标,总酚含量、总黄酮含量、DPPH自由基清除率可作为鉴别不同品种食醋的指标。  相似文献   

4.
    
ABSTRACT:  Free radical scavenging activity of 21 tropical plant extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl assay (DPPH). Total phenolic compounds and flavonoids were determined using Folin–Ciocalteu and HPLC, respectively. Results of the study revealed that all the plants tested exhibited excellent antioxidant activity with IC50 in the range of 21.3 to 89.6 μg/mL. The most potent activity was demonstrated by  Cosmos caudatus  (21.3 μg/mL) and  Piper betle  (23.0 μg/mL) that are not significantly different than that of     -tocopherol or BHA.  L. inermis  extract was found to consist of the highest concentration of phenolics, catechin, epicatechin, and naringenin. High content of quercetin, myricetin, and kaempferol were identified in  Vitex negundo ,  Centella asiatica , and  Sesbania grandiflora  extracts, respectively. Luteolin and apigenin, on the other hand, were found in  Premna cordifolia  and  Kaempferia galanga  extracts. Strong correlation ( R  = 0.8613) between total phenolic compounds and total flavonoids ( R  = 0.8430) and that of antioxidant activity of the extracts were observed. The study revealed that phenolic, in particular flavonoids, may be the main contributors to the antioxidant activity exhibited by the plants.
Practical Application: Potent antioxidant from natural sources is of great interest to replace the use of synthetic antioxidants. In addition, some of the plants have great potential to be used in the development of functional ingredients/foods that are currently in demand for the health benefits associated with their use.  相似文献   

5.
6.
Five commercial lager beers were used to investigate the evolution of antioxidant activity and ageing compounds during storage. The relationships between antioxidant activity and ageing compounds were clarified by principal component analysis (PCA). Results showed that total phenolic content (TPC) and antioxidant activities of beers evaluated by different assays gradually decreased by 16.08–22.71% (average of 18.56%) and 1.99–78.79% (averages of 24.44–70.50%), which was accompanied by 100.50–269.35% (average of 178.77%) and 11.11–57.14% (average of 28.73%) of increases in total ageing compounds and thiobarbituric acid (TBA) value, respectively, during 6 months of storage at 25 °C. Results from PCA elucidated that there were negative correlations between antioxidant activities and ageing compounds of beers during storage, and satisfactory discrimination among beers with different storage time could be achieved on the basis of antioxidant activities and typical ageing compounds selected.  相似文献   

7.
    
ABSTRACT:  The effects of soaking, boiling, and steaming processes on the phenolic components and antioxidant activity of black beans were investigated. All processed beans exhibited significantly ( P < 0.05) lower antioxidant activities than raw beans in total phenolic content (TPC), DPPH free radical scavenging activity (DPPH), and oxygen radical absorbing capacity (ORAC). Steaming processes resulted in a greater retention of TPC and ORAC values than the boiling processes. Pressure boiling shortened processing time compared to regular boiling, resulted in insignificant differences in TPC, but significantly increased in ORAC as compared to the regular boiling method. Pressure steaming resulted in significant decreases in TPC, DPPH, while significantly increased in ORAC compared to regular steaming. Greater TPC, DPPH, and ORAC values were detected in boiling water than in the soaking and steaming water. Mass balance analysis showed that boiling caused more dry solid loss than steaming. All of these results indicated that processing methods significantly changed contents and activities of antioxidant components of black beans. Steam processing exhibited several advantages in appearance and texture of the cooked product, shortening processing time, and in greater retention of TPC and antioxidant activities. Steam processing may be used to develop high-quality health-promoting black bean products.  相似文献   

8.
9.
酚类物质具有预防疾病和抗氧化等功效,在食品中应用广泛,但是食品烹调手段可能会影响酚类物质的组成和特性,微波加热是新兴的食品烹调加工方法,本文对微波引起食品中酚类物质和食品抗氧化活性的影响进行了综述,展望了酚类提取物的应用情况。   相似文献   

10.
花色苷类色素抗氧化活性研究进展   总被引:2,自引:0,他引:2  
天然色素花色苷具有较强的抗氧化活性,本文对花色苷的抗氧化活性检测方法进行了总结,主要对体外方法像清除DPPH·的能力、ABTS+,ORAC等进行介绍,为进一步研究花色苷类色素的抗氧化活性提供便利.  相似文献   

11.
食醋抗氧化性初步研究   总被引:2,自引:0,他引:2  
李坚  刘东波  夏志兰 《中国酿造》2010,29(5):106-108
测定了不同品种食醋中总酚、总黄酮含量及DPPH自由基清除活性,结果显示,不同品种食醋中均含有丰富的总酚和总黄酮,其中香醋中含量最高,总酚、总黄酮含量分别达到2.212mg/ml和1.623mg/ml,白醋含晕最低,总酚、总黄酮含量分别达到0.604mg/ml和0.4138mg/ml,因此,总酚含量、总黄酮含量、DPPH自由基清除率可作为评价食醋抗氧化性的指标,来鉴别不同品种食醋.  相似文献   

12.
皇冠果抗氧化活性研究   总被引:1,自引:0,他引:1  
采用氧自由基吸收能力(oxygen radical absorbance capacity,ORAC)法和二苯基苦基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法,研究了皇冠果果肉和果核提取液的抗氧化能力.结果显示,ORAC法得出皇冠果肉与果核的平均ORAC值分别为934.OOμmol TE/g和375.59μmol TE/g,DPPH法得出不同浓度皇冠果肉和果核提取液的自由基清除率分别为80.58‰90.24%和65.26%~79.12%.结果表明,皇冠果肉和果核均有明显的抗氧化活性,果肉的抗氧化能力明显高于果核的抗氧化能力.  相似文献   

13.
    
Vinegars contain several bioactive compounds that are characterized according to the type of the raw material, such as grape vinegars and apple vinegars. Liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry was used for identification and quantification of phenolic compounds. Antioxidant properties of vinegars were determined by 2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐azino‐bis‐3‐ethylbenzthiazoline‐6‐sulphonic acid assays. Antimicrobial activities of vinegars were examined with an agar disc diffusion method with Staphylococcus aureus , Escherichia coli and Pseudomonas aeruginosa . Gallic acid and chlorogenic acid were found to be the major phenolic acids accounting for the largest proportion of the total phenolic acid contents in grape vinegars and apple vinegars. Within the flavonols, quercetin‐3‐O‐galactoside and quercetin were detected as the major compounds in grape vinegars. Apple vinegars were characterized by phloridzin, phloretin and high chlorogenic acid content. Antimicrobial activity results indicated that grape vinegars exhibited higher antimicrobial activity against tested bacterial strains correlated with their higher antioxidant capacity. Copyright © 2017 The Institute of Brewing & Distilling  相似文献   

14.
以ORAC法为评价指标优化制备大豆抗氧化肽   总被引:4,自引:0,他引:4  
以大豆分离蛋白为原料,用ORAC(oxyen radical absorbance capacity)法对3种商业蛋白酶(Alcalase、Neutrase、Pepsin)的酶解产物进行抗氧化活性评估,以制备具有抗氧化能力的大豆肽。结果表明:在其适宜酶解条件下,Alcalase酶解产物的ORAC值最高,Neutrase次之,而Pepsin酶解产物的最低。Alcalase酶解产物ORAC最高值可达Trolox当量1900.48μmol Trolox equivalent/g Peptide,谷胱甘肽当量1711.91mg Glutathione equivalent/g Peptide,说明该条件下所制备的大豆肽具有非常好的抗氧化活性。同时,研究发现,利用ORAC法测定大豆肽的抗氧化活性结果与DPPH(1,1-Diphenyl-2-Picrylhydrazyl)法测定的结果具有显著的相关性。  相似文献   

15.
The total sulphur dioxide (SO2) concentration of beers decreases during storage, essentially following first-order kinetics. Beers differ in the rate at which they lose SO2. Half-lives for total SO2 loss in 22 commercial beers stored at 25°C ranged from 37·7 days to more than 1000 days. The rate of loss could be predicted from beer compositional data using partial least squares (PLS) regression modelling. Initial concentrations of total SO2, sugars and ions were important factors in the models.  相似文献   

16.
不同品种甘薯汁抗氧化活性的研究   总被引:1,自引:0,他引:1       下载免费PDF全文
研究了9个品种甘薯汁的酚类物质及其抗氧化活性,旨在为甘薯汁的开发利用提供参考依据。分别采用Folin-酚法、亚硝酸钠法、pH示差法测定了甘薯汁的总酚、总黄酮、总花色苷含量。结果表明,甘薯汁中含有丰富的酚类物质,不同品种甘薯汁的总酚、总黄酮和总花色苷含量存在差异。通过4个体外抗氧化体系(FRAP法、DPPH自由基法、ABTS+自由基法、OH自由基法)评价了甘薯汁的抗氧化能力。结果显示,不同品种甘薯汁所呈现的抗氧化能力不同,酚类物质含量较高的甘薯汁其抗氧化能力较强。从抗氧化能力方面考虑,杭引薯1号、杭引薯3号、浙紫薯1、13号这4个抗氧化能力较强的品种较适合用于果汁加工以及功能性饮品的开发。   相似文献   

17.
赵金伟  李范洙  张先 《食品科学》2010,31(17):170-172
通过对DPPH 自由基、羟自由基(·OH)和亚硝酸根离子的清除能力实验,测定苹果梨幼果和成熟果果皮中提取的酚类物质抗氧化活性。结果表明:苹果梨酚类物质对DPPH 自由基、·OH 和亚硝酸根离子均有较好的清除效果,其中幼果中提取的酚类物质清除效果要好于成熟果果皮。  相似文献   

18.
蜂蜜中酚类物质及其抗氧化活性研究进展   总被引:1,自引:0,他引:1  
穆雪峰  徐响  孙丽萍  庞杰  沈新锋  黄兰  何伟 《食品科学》2011,32(21):278-282
酚类物质是蜂蜜中重要的生物活性成分,包括酚酸、黄酮及其衍生物,具有抗氧化活性,可以清除超氧阴离子自由基、过氧亚硝酸盐自由基、脂质过氧化自由基、DPPH自由基、ABTS+ ·、羟自由基等。本文综述了国内外蜂蜜中酚类物质的提取、检测、主要成分及其抗氧化活性的研究进展,旨在为蜂蜜中酚类物质的开发及应用提供依据。  相似文献   

19.
全谷物酚类化合物抗氧化活性研究进展   总被引:4,自引:0,他引:4  
李富华  郭晓晖  夏春燕  陈龙  令博  明建 《食品科学》2012,33(13):299-304
全谷物中含有多种生物活性成分,如酚类化合物、β-葡聚糖、膳食纤维等,其中,酚类化合物以其独特的抗氧化功能而备受人们的青睐。本文综述全谷物酚类化合物的组成、存在形式、影响其含量的因素及其抗氧化效果的评价方法,旨为全谷物酚类化合物的研究提供参考。  相似文献   

20.
    
In this work we have determined the phenolic composition of raw cowpeas (Vigna sinensis L) of the variety Carilla by HPLC/PAD/MS and have studied the effect of fermentation, both spontaneous and with Lactobacillus plantarum ATCC 14917, on the phenolic compounds. This variety contains mainly ferulic and p‐coumaric acids esterified with aldaric acids, together with the cis and trans isomers of the corresponding free acids. Hydroxybenzoic acids such as gallic, vanillic, p‐hydroxybenzoic and protocatechuic were also found, along with flavonols such as a myricetin glucoside, mono‐ and diglycosides of quercetin and a quercetin diglycoside acylated with ferulic acid. Fermentation, both spontaneous and inoculated, modifies the content of phenolic compounds, but differently in each case. The antioxidant activity as free radical‐scavenging activity has also been evaluated. Fermentation followed by heating has been shown to be a very effective process to increase the functionality of this variety of V sinensis. For this reason, this cowpea variety could be used as an ingredient to obtain high value‐added flours. Copyright © 2004 Society of Chemical Industry  相似文献   

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