赣南脐橙皮番茄红素提取前皂化工艺

探讨赣南脐橙皮粉末的番茄红素提取前最佳皂化工艺。以赣南脐橙皮粉末为原料,0.5 mol/L碳酸钠溶液为皂化剂,采用分光光度法测定样品番茄红素含量,通过单因素试验探讨料液比、皂化时间、皂化温度对番茄红素提取的影响;然后通过正交试验得出最佳皂化工艺。结果表明,在皂化工艺中对番茄红素提取的影响程度大小依次是料液比、皂化时间、皂化温度;最佳皂化工艺为料液比1∶10(g/mL)、皂化时间40 min、皂化温度20℃。在最佳皂化条件下,番茄红素提取量为17.14 mg/100 g。因此,在最佳皂化条件下能从赣南脐橙皮粉末中提取更多的番茄红素。     

响应面法优化类球红细菌中番茄红素提取工艺

利用响应面法研究有机溶剂提取类球红细菌中番茄红素的工艺。以每克菌体提取所得番茄红素的量作为响应值,在单因素试验基础上,选取皂化时间、料液比、超声时间及提取次数为自变量,采用响应面法研究各因素及其交互作用对类球红细菌番茄红素提取量的影响并建立了番茄红素提取的回归模型,确定了番茄红素提取工艺的最佳条件为以丙酮为提取溶剂、皂化时间28 min、料液比1∶86（g/mL）、超声时间11 min、提取次数2 次。在此条件下,番茄红素提取量为9 326.48 μg/g。本实验所得工艺方法切实可行,为类球红细菌工业化发酵生产番茄红素的进一步研究提供技术参考。     

番茄的不同预处理对番茄红素提取效率的影响

考察了番茄的4种不同预处理及4种再处理方法对番茄红素提取效率的影响,结果表明,在番茄的4种不同预处理中,直接打浆的方法番茄红素的提取量最大,其次为打浆后离心脱水,再次为打浆后离心脱水再烘干,打浆后直接烘干提取量最低,而打浆后离心脱水,番茄红素提取效率相对高;番茄再处理中的番茄红素的提取量大小顺序为加果胶酶、皂化、微波、加BHT,但这4种预处理方法都大于对照组。     

番茄红素的提取及其在大豆油中稳定性、抗氧化性的研究

用有机溶剂从番茄原料中提取番茄红素油树脂,采用皂化法与重结晶相结合的方法来纯化番茄红素油树脂得到番茄红素晶体。考察了时间、温度、光照对溶于大豆油中的番茄红素稳定性的影响;并对不同添加量番茄红素对大豆油的抗氧化性进行了实验。结果表明,番茄红素在大豆油中的保存率随着贮存时间的延长而降低,低温避光贮存比高温光照贮存的保存率高。高添加量番茄红素对大豆油的抗氧化效果显著。     

原料前处理方法对番茄红素提取的影响

采用碱皂化法、加酶法和加酶皂化结合法对番茄酱进行了前处理,考察了原料前处理方法对番茄红素提取的影响。结果显示,碱皂化法、加酶法和加酶皂化结合法处理原料每公斤番茄酱的番茄红素得率分别为131.4、189.1、112.8mg。其中加酶法处理原料效果最好,番茄红素得率是原料未经处理的2.5倍。     

皂化对番茄红素提取的影响研究

用KOH溶液对番茄进行碱洗皂化可有效的除去番茄中的大部分脂肪酸甘油酯及各种游离脂肪酸 ,释放出其中的番茄红素。番茄皂化的最佳条件为 :KOH浓度 0 5mol/L ,温度 65℃ ,皂化时间 0 5h。皂化后的番茄经固液萃取后得到的番茄红素油树脂产品有部分晶体出现 ,色价提高到 40 2 5。     

赣南脐橙皮皂化后番茄红素提取工艺

探讨赣南脐橙皮粉末皂化后番茄红素提取最佳工艺。以赣南脐橙皮粉末为原料,0.5 mol/L碳酸钠溶液为皂化剂,乙酸乙酯为提取剂,采用分光光度法测定样品番茄红素含量。通过单因素试验探讨料液比、提取温度、提取时间对番茄红素含量的影响;然后通过正交试验得出最佳提取工艺。结果在提取工艺中对番茄红素含量的影响程度大小依次是料液比、提取温度、提取时间;最佳提取工艺为料液比(g/mL)1∶20、提取温度70℃、提取时间40 min。在最佳提取条件下,番茄红素提取量为18.63 mg/100 g。在最佳提取条件下能从赣南脐橙皮粉末中提取更多的番茄红素。     

秋橄榄果实中番茄红素有机溶剂的萃取及其稳定性研究

利用有机溶剂萃取秋橄榄中的番茄红素,对影响萃取的诸因素,如萃取剂的种类、料液比、萃取温度和萃取时间等进行研究,并用响应面法优化提取工艺条件;另外,还研究了温度、光照、金属离子对秋橄榄番茄红素稳定性的影响。结果表明:用丙酮作萃取剂时,秋橄榄果实中番茄红素的提取效果最佳,优化后的萃取温度为52℃,萃取时间为2.1 h,料液比为1:21(g:mL)。验证实验发现,萃取量为(218.03±9.10)μg/g鲜重,与预测值较接近。秋橄榄果实中的番茄红素在高温、阳光直照、Fe3+存在的条件下十分不稳定。     

皂化及微波法提取番茄红素的工艺

研究皂化及微波处理法对番茄红素提取效果的影响.采用正交试验优化皂化液浓度和微波处理功率等条件,结合考虑生产应用的经济性,确定番茄红素提取的最优工艺条件为:NaOH皂化溶液浓度0.6mol/L、微波处理功率为300 W、微波处理时间为80 s、液固比为3:1(mL:g),提取率可达65.33%.     

响应面法优化粗糙脉孢菌番茄红素的皂化提取工艺及其抗氧化活性

本文研究了皂化反应结合有机溶剂对粗糙脉孢菌番茄红素提取的影响。通过单因素实验和响应面法对皂化提取条件进行了优化,包括皂化温度、皂化时间及NaOH浓度;同时以β-胡萝卜素、V_C及V_E为对照,测定了粗糙脉孢菌番茄红素对DPPH·、O₂-·、·OH三种自由基的清除能力,并通过FRAP法测定其抗氧化能力。结果表明,最佳皂化工艺条件为:皂化温度58 ℃、皂化时间21 min、NaOH浓度0.06 mol/L,在此条件下番茄红素得率为(1.620±0.011) mg/g,比未皂化时提高了38.46%,抗氧化试验结果表明粗糙脉孢菌番茄红素具有良好的体外抗氧化活性,当其浓度为250 μg/mL时对DPPH·及O₂-·的清除活性最强,分别为86.43%、90.21%;当其浓度为1200 μg/mL时,对·OH的最大清除率为85.83%。     

番茄红素提纯工艺条件的研究

以番茄红素油树脂为原料,利用皂化法和重结晶法作为主要提纯工艺,纯化得出高含量的番茄红素晶体。通过单因素及正交试验,确定番茄红素提纯工艺的最佳提取条件为番茄红素油树脂∶丙三醇∶氢氧化钾∶无水乙醇比例4∶3∶1∶1（V/V）,皂化温度70 ℃,皂化时间2.5 h,重结晶时间48 h,在此条件下番茄红素纯度达到61.64%。 关键词：中图分类号：TS264.2 文章编号：0254-5071（20１6）03-0111-03 doi:     

Optimisation of biological and physical parameters for lycopene supercritical CO2 extraction from ordinary and high‐pigment tomato cultivars

BACKGROUND: Lycopene is used for several industrial applications. Supercritical CO₂ (SC‐CO₂) extraction from red‐ripe tomato fruits is an excellent technique to replace the use of harmful solvents. In this study, starting from red‐ripe tomatoes of ordinary and high‐lycopene cultivars, the effect of different agronomical and technical aspects on lycopene content, stability and yield was evaluated throughout the production process from fresh tomatoes to the final SC‐CO₂‐extracted oleoresin containing lycopene. RESULTS: Red‐ripe tomato cultivars differed in their lycopene content. Irrigation excess or deficit caused an increase in the amount of lycopene in the fruits. Fresh tomatoes were processed into a lyophilised matrix suitable for SC‐CO₂ extraction, which could be stored for more than 6 months at ? 20 °C without lycopene loss. Under the optimal extraction conditions, efficiencies of up to 80% were achieved, but the recovery of lycopene in the extracted oleoresin was very low (～24%). Co‐extraction of the tomato matrix mixed with a lipid co‐matrix allowed the recovery of ～90% of lycopene in the oleoresin. Using the high‐lycopene cultivars, the yield of total extracted lycopene increased by ～60% with respect to the ordinary cultivars. Lipids and other biologically active molecules were present in the oleoresin. CONCLUSION: A method for extracting, from a tomato matrix, a natural and solvent‐free oleoresin containing lycopene dissolved in a highly unsaturated vegetable oil has been described. The oleoresin represents an excellent product for testing on cancer and cardiovascular disease prevention. Copyright © 2010 Society of Chemical Industry     

番茄红素提取工艺及其性质比较

用番茄作原料,以V(丙酮):V(水)=10:1的丙酮溶液作为提取溶剂,以测定番茄红素紫外扫描光谱为依据,研究了溶剂浸提法、微波提取法提取番茄红素的工艺条件及番茄红素性质.结果表明:微波提取法比溶剂浸提法有较高的提取率,并且节省时间和提取试剂;番茄红素在472nm波长处有最大吸收峰;番茄红素对光、强酸、高温、Fe3+和Cu2+不稳定,对氧化剂、还原剂、弱酸、碱等较稳定.     

Supercritical carbon dioxide extraction of lycopene: A review

Lycopene is a powerful antioxidant and potential high-value compound for the nutraceutical and pharmaceutical sectors. Its low solubility in water and high solubility in toxic organic solvents has led to a number of studies being carried out on using supercritical carbon dioxide to extract lycopene, with a majority of the studies focusing on extraction from tomatoes or tomato-derived preparations. Parameters such as temperature, pressure, flow rate, extraction time, sample moisture content and sample particle size influence the yield of lycopene via the exertion of different mechanisms. The use of co-solvents and modifiers can greatly enhance the lycopene yield beyond optimization of the parameters previously mentioned, while the co-extraction method offers a new way of enhancing lycopene yields. The scope of this review covers the effects of various parameters on lycopene extraction by supercritical carbon dioxide.     

Enzyme aided extraction of lycopene from tomato tissues

Lycopene is a natural carotenoid pigment and a high value nutraceutical having wide use. The objective of the present work was to obtain a good yield of lycopene from tomato tissues, using cellulase and pectinase enzymes. Various parameters such as concentration of enzymes and time of incubation were optimised, to improve the yield of lycopene from tomatoes. Enzyme aided extraction of lycopene from whole tomatoes under optimised conditions resulted in an increase in the lycopene yield by 132 μg/g (198%) in cellulase treated sample and 108 μg/g (224%) in case of pectinase treated sample. Extraction from tomato peel under optimised conditions showed a remarkable increase in the yield of lycopene by 429 μg/g (107%) and 1104 μg/g (206%), for cellulase and pectinase treated samples, respectively. Likewise, the enzyme aided extraction of lycopene from fruit pulper waste and industrial waste of tomatoes was done to determine the potential for recovering the natural pigment from tomato waste.