酪蛋白磷酸肽(CPP)对生长期大鼠骨密度的影响

探讨4种不同酪蛋白磷酸肽（CPP）的不同CPP/Ca比值对生长期大鼠骨密度的影响.选用4周龄雄性SD大鼠,按体重随机分为低钙组、补钙组和CPP加钙实验组（A,B,C,D四种CPP按CPP/Ca比值为1,2,4,分别设低、中、高3个剂量组）,以灌胃方式给予补钙和CPP,12周后处死大鼠,取双侧股骨,称重,计算股骨指数;化学法测骨钙,双光能X线吸收法测骨密度.结果表明,4种CPP三个剂量组和补钙组大鼠骨密度均显著高于低钙组（P＜0.01）,其中C种CPP高剂量组的骨密度显著高于补钙组（P＜0.05）,其余各组CPP的骨密度也高于补钙组,但差异不显著.C种CPp在CPP/Ca比值为4时能显著促进大鼠钙的吸收和利用,提高股骨骨钙含量,增加骨密度,其他3种CPP增加骨密度的作用不显著.     

钙吸收增效剂酪蛋白磷酸肽

报导了人群缺钙需补钙的情况，介绍了钙吸收增效剂酪蛋白磷肽的制备方法，作用机理和实际应用的效果。     

生物活性物质：酪蛋白磷酸肽

生物活性物质：酪蛋白磷酸肽朱迅涛（无锡轻工大学·无锡市２１４０３６）关键词：酪蛋白磷酸肽，钙ＢｉｏａｃｔｉｖｅＦｏｏｄｓｔｕｆｆ：ＣａｓｅｉｎＰｈｏｓｐｈｏｐｅｐｔｉｄｅ￥Ｋｅｙｗｏｒｄｓ：ＣＰＰＣａｌｃｉｕｍ随着全球人口老龄化的倾向，老年人骨质疏松...     

酪蛋白磷酸肽、木糖醇等成分对乳铁蛋白抗大肠杆菌活性影响

乳铁蛋白是一种铁结合糖蛋白，具有多种生物活性功能。本文对酪蛋白磷酸肽(CPPs)、木糖醇、VC、低聚异麦芽糖等功能性食品常用成分对乳铁蛋白的抗大肠杆菌性能的影响进行研究。在研究过程中，通过对这些物质在不同浓度下对乳铁蛋白最小抑菌浓度影响变化比较，揭示了CPPs、木糖醇、VC、低聚异麦芽糖等成分对乳铁蛋白的抑制大肠杆菌作用的影响表观现象，结果表明木糖醇及VC对乳铁蛋白的抑菌作用有明显的增强作用。而CPPs和低聚异麦芽糖对乳铁蛋白的抑菌作用有减弱作用。     

酪蛋白磷酸肽的体外功能试验

对不同级别的酪蛋白磷酸肽（Casein Phosphopeptide,以下简称CPP）促进钙吸收的体外功能性质进行了研究,测定了CPP的结合钙量、CPP阻止磷酸钙沉淀形成的效果及持钙能力.结果表明CPP对钙的结合量随温度的升高而减少,随pH值的升高而增加;CPP对阻止磷酸钙沉淀的作用随温度升高和溶液钙磷比增加而下降;随氮磷摩尔比降低,CPP阻止磷酸钙沉淀形成的最低有效浓度下降;对于不同的钙磷比,CPP的氮磷摩尔比越小,保持在溶液中的钙量越多,但每摩尔磷保持钙的摩尔数随氮磷摩尔比的降低而降低.     

酪蛋白磷酸肽促进人体钙吸收的作用研究

报道了酪蛋白磷酸肽（ＣＰＰ）对人体钙吸收的影响。人体实验结果表明：实验组口服含ＣＰＰ的钙片后，２４ｈ尿钙均值达７６５．８＋２３４．２ｍｇ，比相应对照组的６３９．８＋２５３．８ｍｇ尿钙浓度提高近１９．７％。二者差异显著（Ｐ＜０．０５）。说明酪蛋白磷酸肽能显著促进人体胃肠道对食用钙的吸收。     

酪蛋白磷酸肽持钙能力的研究

通过体外模拟实验考察酪蛋白磷酸肽( CPP)阻止磷酸钙沉淀的能力,分析了水解度、CPP添加量、CPP的不同等级以及食品加工过程中的其他因素对CPP持钙能力的影响,为CPP功能特性的评价以及在食品中的应用提供了依据.     

模拟消化过程中酪蛋白磷酸肽持钙能力的研究

本文研究了在模拟消化过程中酪蛋白磷酸肽(Caseinophosphopeptides,CPP)抑制磷酸钙沉淀产生(即持钙能力)的作用,并在相同处理条件下对含CPP钙片A与不含CPP钙片B的持钙能力进行比较.结果表明,在弱碱性溶液中含CPP的钙片A能有效地延缓磷酸钙沉淀的产生.而不含CPP的钙片B则无此作用,但当钙片B被加入一定量的CPP后,同样能缓磷酸钙沉淀的产生.     

酪蛋白磷酸肽(CPPs)的分子量及持钙功能的分析

实验对不同来源的酪蛋白磷酸肽（CPPs）的氮与磷的摩尔比值（N：P）、分子大小及结合钙的最大量进行了分析和测定，初步探讨了CPPs的N：P、分子大小和结合钙的能力之间的关系。N：P较小则CPPs制品的纯度较高或磷酸基密度较大，也即CPPs制品中分子量较小部分占的比例较大，结合钙的能力也较强。N：P能较客观和综合地反映CPPs的肽链长度和磷酸基密度，因而可以作为CPPs制品的特性指标。     

酪蛋白磷酸肽-钙络合物对酸乳贮藏特性的影响

为探究酪蛋白磷酸肽-钙（CPP-Ca）络合物对酸乳贮藏特性的影响,研究CPP-Ca络合物不同添加量对酸乳21 d贮藏期内乳酸菌总数、pH值、滴定酸度、乳清析出率及黏度的影响。与对照组比较,当CPP-Ca络合物添加量为0.15 g/100 mL时,酸乳中乳酸菌存活率从32.92%提高至47.15%（P＜0.05）;滴定酸度增长率从9.96%降低至7.06%（P＜0.05）;乳清析出率降低了0.4%（P＜0.05）,黏度比提高了5 729 mPa·s （P＜0.05）。结果表明,CPP-Ca络合物的添加能增加酸乳贮藏期内的乳酸菌总数,延缓酸度积累,降低乳清析出率,增加黏度,改善酸乳贮藏期内的产品品质。     

Semi-preparative isolation of phosphopeptides derived from bovine casein and dephosphorylation of casein phosphopeptides

Semi-preparative isolation of casein phosphopeptides (CPP) from a tryptic hydrolysate of bovine casein was performed applying a three-step procedure consisting of solid phase extraction, reversed phase HPLC and ion exchange chromatography. Dephosphorylation of CPP was achieved using immobilized alkaline phosphatase. The purified phosphopeptides and their dephosphorylated forms obtained by these methods are suitable for comparative studies on biological activities, especially mineral binding and immunmodulation.     

酪蛋白磷酸肽(CPP)理化特性的研究

研究了CPP的溶解性、起泡性及乳化性。CPP在pH2.0～10.0范围内,溶解性除在pH4.0约为90％外,其它均高于90％,且随pH增加而增大。与酪蛋白相比,CPP具有更好的起泡性和泡沫稳定性。CPP对热及Ca²⁺具有较好的稳定性。CPP乳化力较酪蛋白下降约20％,乳化性下降2.89％,乳化稳定性下降1.45倍。      

Microdetermination of phosphorus from infant formulas, casein and casein phosphopeptides

A spectrophotometric method, which has been proposed for the determination of phosphorus in biological fluids based on the formation of a phosphomolybdate complex, is adapted and validated for the determination of phosphorus in milk-based infant formulas, casein, casein phosphopeptides (CPPs) and the soluble fractions resulting from their gastrointestinal digestion, as well as in the fractions resulting from the ion-exchange high-performance liquid chromatography (IE-HPLC) of CE90CPP and in the soluble fraction of infant formula. The detection and quantification limits (1.1 and 3.6 mg P/100 g sample, respectively) are low enough for the purpose described. The linearity (from 0.1 to 8 g of phosphorus in the assay) is adequate. The precision of the method, expressed as relative standard deviation, is lower than 1%, and the accuracy checked by the analysis of SRM 1846: milk-based powdered infant formula is good. The quality of the method, together with the low cost and ease of use, makes it suitable for routine analysis.     

Effect of casein phosphopeptides on bioavailability of the alimentary minerals

Caseinophosphopeptides (CPPs) are phosphorylated casein-derived peptides which possess the ability to bind and solubilise minerals, such as Ca2+. The high bioavailability of Ca2+ fram milk and dairy products has been attributed to the production of CPPs which are produced following digestion of casein by the action of gastrointestinal proteinases. CPPs, which appear to be resistant to extensive proteolytic degradation, accumulate in the distal small intestine where they are purported to play a role in enhancing the passive absorption of Ca2+ and other trace elements. A recent human feeding tral reported improved Ca2+ and Zn2+ absorption following CPPs incorporation into food. This review summarises the production, characterization and potential applications of CPPs.     

不同水解度酪蛋白磷酸肽的理化性质和持钙能力比较

目的 研究不同水解度(degree of hydrolysis, DH)酪蛋白磷酸肽(casein phosphopeptide, CPP)的理化性质和持钙能力。方法 实验室自制得到水解度分别为4.32%～26.76%的CPP样品,分别测定CPP含量[高效液相色谱法(high performance liquid chromatography, HPLC)和钡-乙醇沉淀法]、等电点不溶物含量、相对分子质量分布和持钙能力。结果 随着水解度的上升,通过HPLC测定的CPP标志峰的峰面积随之增加,等电点不溶物含量随之减少,而通过钡-乙醇沉淀法得到的CPP含量无明显变化,可将等电点不溶物含量作为评价产品质量的指标;目前的相对分子质量分布检测方法对不同水解度的CPP样品检测误差较大,需使用酸溶蛋白含量作为校正系数进行校正后方可使用;当CPP的质量浓度为0.6～0.8mg/mL时,水解度为9.22%和15.06%的CPP的持钙能力效果优于其他水解度的CPP。结论 水解度对酪蛋白磷酸肽的理化性质和持钙能力有明显的影响,在实际应用时应选用合适的水解度和检测方法。     

Effect of combined vitamin D and E deficiencies on calcium metabolism and bone tissue of the rat

In experiments on young rats a study was made of the influence of combined vitamin D and E deficiency on biochemical signs of rickets. It was shown that in intact animals vitamin E deficiency induced moderate hypocalcemia, a tendency to decreased Ca active transport in the small intestine and mineral saturation of the bone tissue, as well as a significantly increased Ca content in the skeletal muscles and heart. The concentration of 25-oxyvitamin D (25-OVD) in the blood serum of these animals was not changed. Combined vitamin D and E deficiency intensified disorders typical for rickets; sometimes synergy of such a negative effect was recorded. A delay in the recovery of Ca metabolism parameters, bone tissue condition and 25-OVD circulating concentration persisted, when the animals with vitamin D and E deficiency were injected cholecalciferol during 6 days. The data obtained have evidenced a possible role of vitamin E deficiency in the rickets development, that is, probably, mediated, to a certain extent, by its influence on metabolism and (or) biochemical function of vitamin D.     

响应曲面法优化酪蛋白磷酸肽的制备

采用Plackett-Burman设计法和响应面分析法相结合,以CPP（酪蛋白磷酸肽）得率作为指标,进行碱性蛋白酶水解酪蛋白的条件优化。结果表明,底物浓度、温度和pH是影响CPP得率的主要因素。最佳水解条件为温度55℃、底物浓度16%、pH10.8、酶底比1.5%,水解时间4.5h,在此条件下,CPP得率可达33.2mg/mL。