Prolactin-releasing effect of buspirone in developing and adult male and female rats

The prolactin-releasing effects of buspirone, an azaspirodecanedione anxiolytic drug unrelated to the benzodiazepines in structure and pharmacologic properties, was examined in developing and adult male and female rats. The possibility that effects of this drug on hormone release could be modulated by neonatal brain sexual differentiation was also evaluated. A single injection of buspirone, 2 or 10 mg/kg body wt, increased serum prolactin (PRL) levels in both sexes; the increase was significant from Day 12 onward. The PRL-releasing effect increased with age. No significant sexual differences were observed in younger rats, but in peripubertal and adult animals, the hyperprolactinemic response was higher in the female. Neonatal androgenization of females or orchidectomy of males failed to modify the PRL-releasing action of buspirone. Serum titers of luteinizing hormone and follicle-stimulating hormone were not modified by buspirone at any age. The present results show for the first time the ontogeny of the PRL-releasing effect of buspirone in male and female rats, and provide evidence that the response is higher in the female and that the effect does not depend on brain sexual differentiation.     

Endogenous reproductive hormones and nocturnal rhythms in partner preference and sexual behavior of ATD-treated male rats

Male rats received subcutaneously silastic capsules, containing the aromatase inhibitor 1,4,6-androstatriene-3,17-dione (ATD), shortly after birth. Control males were given silastic capsules containing cholesterol. The capsules were removed at the age of 21 days. In adulthood, blood serum was collected early and late in the dark phase of the light/dark cycle (experiment I). Testosterone and luteinizing hormone and follicle stimulating hormone (FSH) fluctuated nocturnally, both in ATD and control males, with highest levels late in the dark phase. FSH levels were significantly higher in ATD males. Nocturnal levels of inhibin, a selective suppressor of pituitary FSH secretion, also fluctuated in both ATD and control males, with lowest levels late in the dark phase. In experiment II, ATD and control males were tested for partner preference behavior in a three-compartment box (choice: sexually active male vs. estrous female) early and late in the dark phase. When gonadally intact, ATD males, but not controls, showed a clear nocturnal rhythmicity in partner preference behavior and sexual behavior. Early in the dark phase, such ATD males preferred the vicinity of and interaction with a sexually active male. Late in the dark phase, this preference for the active male shifted to a preference for the estrous female. Control males preferred the estrous female. After castration and subsequent treatment with testosterone via silastic capsules, which ensured constant blood serum levels, ATD males continued to show their nocturnal rhythms in partner preference behavior and in sexual behavior. Thus, the underlying mechanism of the nocturnal rhythmicity phenomenon is an organizational effect of neonatal ATD treatment rather than an activational effect of fluctuating serum hormone levels.     

Chronic cadmium exposure alters cocaine self-administration in adult male rats.

Adult male rats (Rattus norvegicus) were exposed to a water supply in the home cage containing 100 ppm cadmium chloride and sodium saccharin (.65% wt/vol; cadmium group) or water containing only the saccharin amendment (group control). On Day 65 of exposure, animals from each group received jugular catheter implants and were subsequently trained over the course of 15 daily 2-hr sessions to self-administer a .25 mg/kg/infusion of cocaine HCl under a fixed ratio 1 schedule. Immediately following acquisition training, the full dose-effect function was determined for all animals by using cocaine doses of .03, .06, .125, .25, .50, and 1.0 mg/kg. Cadmium-exposed animals executed more active (cocaine) lever responses during acquisition training but were not different from controls in depressing a pharmacologically inactive lever. For dose-effect testing, cadmium exposed animals exhibited greater self-administration than controls at the higher doses of cocaine, and there was evidence that the cocaine dose that produced maximum responding was higher in cadmium-exposed than control animals. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Ipriflavone does not alter bone apatite crystal structure in adult male rats

We have previously found that a short-term treatment with high doses of ipriflavone increased bone density and improved the biomechanical properties of adult male rat bones, without altering their mineral composition. To determine whether this effect can be associated with alterations of bone crystal structure, we have performed X-ray diffraction analysis of bones obtained from rats treated with ipriflavone at doses that were effective in inducing favorable changes on bone density and biomechanics. Eighteen-week-old male Sprague Dawley rats were treated by oral route with either ipriflavone (200 or 400 mg/kg/day), or its vehicle for 12 weeks. The treatment was well tolerated and body weight increased to the same extent in all animals. As a measure of bone crystallinity, we examined the (310) and (002) reflections of the X-ray diffraction patterns, corresponding to the directions perpendicular and parallel to the c-axis of the crystals, respectively. No major differences were observed between ipriflavone-treated and control animals for the broadening parameter beta(1/2) for (310) and (002) peaks, as well as for lattice parameters. Therefore, a 12-week treatment with ipriflavone at high doses does not induce significant modifications of bone crystallinity. Thus, the positive effect of ipriflavone on bone mineral density appears to be associated with an increased apatite crystal formation rather than an increase of crystal size. These results provide further evidence for the safety and usefulness of ipriflavone in the treatment of osteoporotic syndromes.     

Hormonal regulation of adult partner perference behavior in neonatally ATD-treated male rats.

Male rats, neonatally treated with ATD (1,4,6-androstatriene-3,17-dione), which blocks the aromatization of testosterone into estradiol (E?), were tested for adult partner preference behavior (PPB; estrous female vs active male). Castration caused a decrease in preference for the female partner in all males, with ATD males showing lower preference for the female partner than controls. Long-term castrated males did not show preference for either partner. Precastration levels of PPB in control males occurred after treatment with E? or dihydrotestosterone (DHT) plus E?. DHT alone had no effect on PPB. With E? alone, the ATD males clearly preferred the male partner. When DHT was added, these ATD males showed no preference for either partner or a low preference for the female partner. In conclusion, adult PPB in male rats is activated by endogenous testosterone or by both its metabolites (DHT and E?) or by E? alone. ATD males showed a much lower preference for the female. There was a differential effect of DHT and E?: DHT had no effect, but E? clearly caused ATD to prefer the male partner and control males to prefer the female partner. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Neonatal castration and adult responsiveness to testosterone in male rats: an interstrain comparison

We have previously demonstrated that the spontaneously hypertensive rat (SHR) has a lower central nervous responsiveness to testosterone than its normotensive counterpart the Wistar-Kyoto rat (WKY). The adult psychoendocrine response capacity depends on a neonatal testosterone surge. On that basis, we compared the effects of neonatal endocrine manipulation on the adult responsiveness to testosterone in the SHR, WKY, and yet another breed of Wistar (Wi) male rats. Interstrain differences in testosterone-induced copulatory behavior at three different doses of testosterone propionate (TP) were investigated. Neonatal treatments were as follows: TP (0.25 mg/animal) given on postnatal days (PND) 0, 2, and 4 (SHR and Wi only) or castration PND 0, 10, or 50. Neonatal TP treatment impaired copulatory performance in the adult SHR but not in the Wi. Neonatal castration improved the responsiveness to TP in the SHR but less so in WKY, whereas no evident effects were seen in the Wi. No significant interstrain differences in plasma testosterone were observed 2, 6-12, or 24 h postpartum. The demonstrated interstrain differences suggest not only that the adult responsiveness to testosterone is established on the basis of the neonatal gonadal secretion as such but that this secretion is kept to an optimal level with respect to subsequent hormone-sensitive mechanisms.     

Dose-dependent prolactin releasing activity of arginine vasotocin in intact and pinealectomized estrogen-progesterone treated adult male rats

Estrogen-progesterone (EP) treated adult male rats were injected intravenously (iv) with 0.1, 1 or 10 mug arginine vasotocin (AVT) or Ringers lactate solution. A significant dose-related rise in plasma prolactin was evident 10 min after injection with AVT. In a second experiment, sham-operated or pinealectomized EP-treated male rats were injected iv with 0.1 or 1 mug AVT or diluent. Plasma prolactin was significantly elevated in both sham-operated and pinealectomized groups at both 10 and 20 min post-injection of 1 mug AVT. These results indicate that AVT has prolactin-releasing activity and that this activity is not dependent upon the presence of an intact pineal gland.     

Effects of adolescent nicotine exposure on opioid consumption and neuroendocrine response in adult male and female rats.

Effects of adolescent nicotine exposure on illicit drug consumption and neuroendocrine functioning were examined in adult rats. Nicotine (NIC; 2 doses) or saline (SAL) was administered via osmotic minipumps to 30 male and 30 female adolescent Wistar rats for 19 days. After NIC/SAL cessation, oral opioid consumption was assessed in the home cage for 4 weeks. Plasma corticosterone and ACTH were measured at the end of the experiment. Low-NIC male rats consumed more fentanyl than did high-NIC male rats; opioid consumption among adult female rats was not altered by NIC exposure. Females consumed more fentanyl than did males, regardless of NIC history. NIC exposure increased adult corticosterone and ACTH levels in a dose-dependent manner. Results suggest important effects of adolescent NIC exposure, including altered neuroendocrine status and opioid consumption. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Hydronephrosis in male rats

Section of the right internal spermatic artery and vein where they overlie the ureter in Wistar-related male rats suggests that the right hydronephrosis found in these animals is not a consequence of simple obstruction by the blood vessels. The diverse effects of this operation on testicular weight presumably reflect variation in the relative importance of internal and external spermatic blood supply.     

The role of gonadal steroid receptor activation in the restoration of sociosexual behavior in adult male rats

This work tested the hypothesis that gonadal steroid receptor activation was necessary for the restoration of several sociosexual behaviors (such as copulatory behavior, partner preference, 50-kHz vocalizations, and scent marking) in testosterone-treated gonadectomized male rats. Gonadal steroid receptors were blocked by systemic administration of the antiandrogen hydroxyflutamide, the antiestrogen RU 58668, or both antagonists simultaneously in a restoration paradigm. Inhibiting androgen receptors with hydroxyflutamide blocked the restoration of male copulatory behavior, partner preference (time spent with a sexually receptive female over a nonreceptive female), 50-kHz ultrasonic vocalizations, and scent marking. On the other hand, we did not find that blocking estrogen receptors with RU 58668 inhibited the restoration of copulatory behavior or partner preference in testosterone-treated gonadectomized male rats, even though the level of brain nuclear estrogen receptor occupation was significantly reduced to the level found in gonadectomized males. However, the restoration of scent marking and 50-kHz vocalizations were impaired by RU 58668. Blocking both nuclear androgen and estrogen receptors with the two antagonists simultaneously did not have a greater inhibitory effect than treatment with each antagonist alone. Therefore, the activation of nuclear estrogen receptors is necessary for the restoration of some, but not all, sociosexual behaviors, which are also androgen receptor-dependent. Besides nuclear estrogen receptors, there are additional, but unknown, targets of estradiol that play a role in mediating copulatory behavior in adult male rats. Moreover, the signals from multiple gonadal steroid signaling pathways converge in the regulation of some sociosexual behaviors in adult male rats.     

Hyperactivity in hyposexual male rats

It is widely accepted that some male rats fail to copulate because of a decrease in arousability, measured as decreased general locomotor activity (hypoactivity). This relationship, however, failed to explain an observation made in our laboratory that rats that failed to copulate exhibited increased general locomotor behavior. To directly address this issue, we quantified open-field and male sexual behaviors in 360 rats from two different strains. Twenty-two out of 49 hyposexual males were also hyperactive; this was a significantly greater number than would be expected by chance (p < 0.002, binomial test). Interestingly, only 6 of the 49 hyposexual males were hypoactive; this number was actually significantly smaller than would be expected by chance (p < 0.02). There was no correlation between behavioral measures and plasma levels of testosterone or progesterone. A decrease in selective attention and a failure to be stimulated by amphetamine was apparent in all hyperactive rats--those normal-sexual as well as hyposexual. The hyperactive rats were not hypertensive. We conclude that a significant percentage of hyposexual rats are hyperactive, and that hypoactive rats generally exhibit normal levels of sexual behavior. Decreased selective attention and decreased responsiveness to amphetamine do not explain this result, which is also not related to blood pressure or androgen levels.     

Heritability of personality traits in adult male twins

Personality test data from the California Psychological Inventory were collected on 99 pairs of identical and 99 pairs of fraternal adult male twins. Heritabilities were comuted for all 18 scales and compared to the heritabilities for "pure" scales with overlapping items omitted. Two of the pure scales, Responsibility and Femininity, had zero heritabilities, whereas all of the full scales had moderate to high heritabilities. It was concluded that item overlap has contributed significantly to previous failures to find evidence for the differential heritability of personality traits as measured by the CPI. CPI items were classified into genetic or environmental categories and separate factor analyses of items in these categories revealed more differences than similarities in factor structure. The genetic personality factors included Conversational Poise, Compulsiveness, and Social Ease. Environmental factors included Confidence in Leadership, Impulse Control, Philosophical Attitudes, Intellectual Interest, and Exhibitionism. Compared to the genetic factors, each of the environmental factors accounted for only a very small percentage of the variance.     

Endogenous opioid facilitation of maternal memory in rats.

In rats, contact with pups at parturition establishes a form of maternal memory that enables female rats to respond rapidly to pups in the future. Treatment of pregnant female rats with the long-lasting μ opioid receptor antagonist, β-funaltrexamine (β-FNA), prior to parturition interfered with the establishment of maternal memory. Similar treatment 3 hr postpartum resulted in disrupted retention of maternal memory that appeared nonspecific, with both drug- and vehicle-treated rats displaying a deficit. However, infusion of the opioid antagonist 24 hr postpartum had no effect on the retention of maternal memory tested 7 days later. These findings indicate that the establishment of maternal memory is mediated by endogenous opioid activity around the time of parturition. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The effects of castration on body composition, adipose tissue cellularity and lipid and carbohydrate metabolism in adult male rats

Gonadal hormones affect body composition, food intake, weight gain and serum lipids in numerous species including man. In this study, mature male Sprague-Dawley rats were castrated or sham-operated at 16 weeks of age. During the 6-week observation period with weekly records of food intake and weight gain, these parameters were significantly lower in the castrated group. The decrease in food intake in this group could not account for the difference in body weight between the groups, indicating a lower feed utilisation in the castrates. At sacrifice accessory reproductive organs, the levator ani muscle, thymus and adrenals were dissected for determination of organ weight and histology, revealing significant reductions in the accessory reproductive organs and levator ani of the castrates. The thymus was significantly heavier in the castrated animals. No differences were found in the adrenals. Two of the sham-operated animals had signs of accidental functional castration. The proportion of body cell mass and total lipid of the carcass was the same in both groups. Significant reductions in adipocyte weights were found in the epididymal depots of the castrated rats. Blood samples taken at sacrifice in pentobarbital anaesthesia were analysed for glucose, insulin, triglycerides, cholesterol, FFA, glycerol and protein. Statistically significant reductions in triglycerides and protein were recorded in the castrated animals without any significant changes in the other parameters studied. The results are discussed with reference to the age of castration and the importance of the reduced food intake in castrated animals.     

Social and hormonal factors influencing infanticide and its suppression in adult male Long-Evans rats (Rattus norvegicus).

Six experiments investigated the effects of social experience and hormonal treatments on the rate of infanticide in male Long-Evans hooded rats that were either purchased from a breeder as adults or raised in the laboratory. Results show that males, but not females, purchased from a breeder exhibited infanticide at high rates, whereas laboratory-reared males were less likely to be infanticidal. Sexual experience reduced the rate of infanticide in laboratory-reared males, and cohabitation with a female through pregnancy and lactation (including exposure to pups) inhibited infanticide in previously infanticidal males. 14 days of cohabitation with a pregnant female following copulation inhibited infanticide in males purchased from a breeder, but copulation without cohabitation and copulation followed by cohabitation with a nonpregnant (ovariectomized) female did not inhibit infanticide. Castration of laboratory-reared males in adulthood did not reduce their rate of infanticide, whereas testosterone implants increased the number of males exhibiting infanticide. Findings are consistent with previous research on mice and gerbils in showing that infanticide is not a fixed trait in male rats but is modified by a number of factors, such as a male's association with a pregnant female prior to the birth of her pups. (35 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Blood pressure reactivity in adult male twins.

Examined possible genetic contributions to cardiovascular reactivity by contrasting patterns of association in 82 monozygotic (MZ) and 88 dizygotic adult male twin pairs (aged 21–61 yrs). Systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were recorded during baseline and during a mental arithmetic task. The task produced significant elevations in all 3 cardiovascular measures. Levels of SBP and DBP reactivity were significantly correlated in MZ pairs only. Statistical tests suggest a heritability estimate of about 50% that was marginally significant for SBP and DBP changes during the task. There was no indication of a genetic influence on HR reactivity. Resting level and static task period measures of SBP, DBP, and HR demonstrated statistically significant heritability estimates of 60–80%. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Endogenous CCK inhibits colonic contractions in unrestrained conscious rats

As cholecystokinin octapeptide (CCK-OP) and feeding have been reported to relax the circular muscle contractions of the proximal colon in unrestrained conscious rats under fasting conditions, the, action of cholecystokinin, released after duodenal infusion of a low residual diet of clinimeal, was studied on the motor activity of the proximal colon in unrestrained conscious rats. We used an implantable telemetry system with a miniature strain gauge force transducer introduced into the rat proximal colon. By using a specific radioimmunoassay system for CCK, plasma levels of CCK before and after duodenal infusion of clinimeal (0.5, 1.0, 2.0 ml) were determined at 0, 5, 10, 20, 30, 60 min in the portal blood. The clinimeal infusion caused a significant increase in CCK levels of the portal plasma during 5 and 30 min. This increase was in a dose-dependent manner. In accordance with this increase in plasma CCK, the motor activity of the proximal circular muscle was suppressed significantly. A bolus injection of the CCK A receptor antagonist, loxiglumide, CR 1505 (0.1, 0.5 and 1.0 mg/kg ip), prior to clinimeal blocked the inhibitory action of CCK on the motor activity in a concentration-dependent manner. These data suggest that endogenous CCK released by a residual diet is involved in the mechanism of inhibition of motor activity in the proximal colon.     

Endogenous vasopressin does not mediate hypoxia-induced anapyrexia in rats

The present study was designed to test the hypothesis that arginine vasopressin (AVP) mediates hypoxia-induced anapyrexia. The rectal temperature of awake, unrestrained rats was measured before and after hypoxic hypoxia, AVP-blocker injection, or a combination of the two. Control animals received saline injections of the same volume. Basal body temperature was 36.52 +/- 0.29 degreesC. We observed a significant (P < 0.05) reduction in body temperature of 1. 45 +/- 0.33 degreesC after hypoxia (7% inspired O2), whereas systemic and central injections of AVP V1- and AVP V2-receptor blockers caused no change in body temperature. When intravenous injection of AVP blockers was combined with hypoxia, we observed a reduction in body temperature of 1.49 +/- 0.41 degreesC (V1-receptor blocker) and of 1.30 +/- 0.13 degreesC (V2-receptor blocker), similar to that obtained by application of hypoxia only. Similar results were observed when the blockers were injected intracerebroventricularly. The data indicate that endogenous AVP does not mediate hypoxia-induced anapyrexia in rats.     

Effect of neonatal treatment with MSG (monosodium glutamate) on thyroid of the adult male rats

Percutaneous transluminal angioplasty (PTA) has been well described in the treatment of mesenteric artery stenoses but has met with limited success in ostial lesions. The authors describe a case of a 79-year-old woman diagnosed with chronic mesenteric ischemia associated with a 22-pound weight loss and postprandial pain. The celiac axis and inferior mesenteric artery were occluded. A high-grade, calcified stenosis was present in the proximal superior mesenteric artery. This was treated with primary stent placement using a Palmaz stent deployed from an axillary approach. A brief discussion of mesenteric ischemic and visceral artery PTA is included.     

Shock-elicited copulation and aggression in male rats.

Assigned 112 male Sherman naive albino rats to 12 experimental and 2 no-shock groups. Ss were presented with a receptive female, a nonreceptive female, or a male rat in a whole or 1/2 of a circular chamber. Copulatory and aggressive responses (fighting and attack) were elicited by electric shock applied to the tail. The probability of obtaining either response could be experimentally shifted by manipulating cage size, shock frequency, and sex of the stimulus animal. (PsycINFO Database Record (c) 2010 APA, all rights reserved)