芯片毛细管电泳激光诱导荧光检测系统的设计

介绍了毛细管电泳芯片的构造和原理，提出激光诱导荧光检测系统的设计方案及其主要器件的选择。分析了光电倍增管电压和共焦针孔对检测系统影响，并给出结果。实验表明，该系统主要技术指标达到了设计要求。     

集成毛细管电泳芯片荧光检测的研究

集成毛细管电泳芯片由于其显著的优点而倍受人们的关注。介绍了这种芯片的特点和应用及检测方法 ,从光源、主物镜和共焦针孔等几个方面详细讨论了检测光路的特点 ,并给出了实验结果。     

毛细管电泳与DNA序列分析

本文报导了毛细管电泳基本理论与技术,评述了DNA序列分析意义及其进展     

毛细管电泳的进展

在本综述中简要地介绍了毛细管电泳的发展，毛细管电泳的仪器，微型电泳仪，毛细管电泳检测器，毛细管电色谱，毛细管电泳柱，毛细管电泳的应用和毛细管电泳的手性分离。     

离子色谱、毛细管电泳及毛细管电泳离子色谱发展概况

毛细管电泳离子色谱是近年来发展迅速的一种分离检测技术,在无机阴阳离子检测方面有着广泛的应用前景。文章综述了离子色谱和毛细管电泳的发展概况,阐述了毛细管电泳离子色谱的研究现状。     

基于单片机的微型毛细管电泳芯片检测器的设计

采用共聚焦式激光诱导荧光检测法,设计了一种基于单片机的微型毛细管电泳芯片检测器,利用数字信号处理技术和面向对象的设计方法开发了一套与之匹配的信号处理软件。此检测器能够实现对芯片毛细管电泳信号的实时处理与分析。     

高效毛细管电泳(HPCE)的方法介绍

本文介绍了高效毛细管电泳,主要对缓冲溶液pH值,添加剂的选择和研究了高效和高重复性的方法。     

毛细管电泳法拆分二氧异丙嗪对映体的条件研究

采用毛细管区带电泳法,以咪唑类离子液体l-正乙基-3-甲基咪唑四氟硼酸盐[bmim]BF4)为手性分离添加剂,β-环糊精为手性选择剂,NaH2PO4为背景电解质,分离电压10kV,实现了二氧异丙嗪对映体的分离。考查了[bmim]BF4浓度、β-环糊精浓度、NaH2PO4浓度和分离电压对二氧异丙嗪对映体分离度的影响。当缓冲液中不含[bmim]BF4时,二氧异丙嗪对映体的分离度仅为0.28,当缓冲液中含[mim]BF4时,随着[bmim]BF4浓度的增大,二氧异丙嗪对映体的分离度增大,可达1.2。实验表明[bmim]BF4能够增强β-环糊精的手性拆分能力,对手性拆分有协同作用。     

PET-laminated thin-film capillary electrophoresis chips by sandwiching method

In this article, the sandwiching method, a novel, simple, and cost-effective capillary electrophoresis (CE) chip fabrication approach, is proposed. In this new approach, polymer PET-laminated film rather than the commonly used silicon wafer is chosen to be the material of the chip; a micro CNC machining center is adopted to cut the microchannels; and a temperature-controlled roll laminator is used to bond the microchannels in between two sealing PET films. Separation experiments using red Rhodamine B and blue Xylene Cyanole illustrate that the separation efficiency of the sandwiching CE chip is comparable to the semiconductor-based chip and the conventional capillary. However, the sandwiching method requires much less complicated fabrication processes and is more cost-effective.     

基于自然对流PCR与毛细管电泳技术的牙周病原菌检测

细菌种类的快速识别在牙病防治中具有重要的应用价值。以牙周病原菌为研究对象,建立了DNA快速扩增的自然对流聚合酶链式反应(PCR)方法及毛细管电泳荧光光学检测系统。研究表明,当毛细管有效长度为8cm、电场强度为100V/cm时,50bp DNA ladder在筛分介质为0.5%HEC(羟乙基纤维素)(1 300K)中的分离效果最佳。毛细管电泳结果表明,采用自然对流法可以在25min内在圆柱腔体中实现牙周病原菌的快速PCR。     

Combined AFM and confocal fluorescence microscope for applications in bio-nanotechnology

We present a custom-designed atomic force fluorescence microscope (AFFM), which can perform simultaneous optical and topographic measurements with single molecule sensitivity throughout the whole visible to near-infrared spectral region. Integration of atomic force microscopy (AFM) and confocal fluorescence microscopy combines the high-resolution topographical imaging of AFM with the reliable (bio)-chemical identification capability of optical methods. The AFFM is equipped with a spectrograph enabling combined topographic and fluorescence spectral imaging, which significantly enhances discrimination of spectroscopically distinct objects. The modular design allows easy switching between different modes of operation such as tip-scanning, sample-scanning or mechanical manipulation, all of which are combined with synchronous optical detection. We demonstrate that coupling the AFM with the fluorescence microscope does not compromise its ability to image with a high spatial resolution. Examples of several modes of operation of the AFFM are shown using two-dimensional crystals and membranes containing light-harvesting complexes from the photosynthetic bacterium Rhodobacter sphaeroides.     

荧光与荧光显微镜

从荧光的基础知识入手 ,简略描述荧光星微镜的特点。对荧光显微镜的主要部件 :光源、滤色片和光学系统作了介绍 ,从荧光特性提出设计要求 ,并对透射和落射荧光进行比较     

微流控分析芯片在线尿中蛋白及LDH同工酶检测

利用MEMS(微机电系统)标准光刻、湿法腐蚀以及低温键合技术制作了微流控分析芯片。在自制的紫外吸收微芯片生化分析仪上,利用该芯片对临床妊娠高血压和多发性骨髓瘤患者的尿液样本进行了在线芯片电泳分析,得到了与医院常规诊断方法一致的诊断结果,而此法进行一次分析仅需要2 min,且试剂消耗量少,这是常规方法不能比拟的。也以LDH同工酶标准品为分析对象,对芯片在线同工酶诊断进行了初步研究,初步实现了LDH₁和LDH₅混合样本的片上分离、孵育反应和产物检测。