硅胶表面罗丹明B分子印迹聚合物的制备及其性能研究

以表面接枝乙烯基的硅胶为载体,罗丹明B为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,制备了表面印迹聚合物微球。通过静态吸附对吸附性能进行分析。结果表明,该印迹聚合物对模板分子具有特异吸附性能,并将其应用于固相萃取,测得罗丹明B的加标回收率为94.4%~101.2%,相对标准偏差(n=3)的范围为2.4%~3.4%。     

阿司匹林分子印迹聚合物的制备及分子识别性能研究

以阿司匹林为模板、丙烯酰胺为功能单体制备了能特异性识别阿司匹林的分子印迹聚合物,研究结果表明,该分子印迹聚合物通过特异的氢键作用力对阿司匹林进行识别,静态分布系数为5 65,对阿司匹林与结构类似物如苯、苯甲酸、乙酸苯酯、水杨酸的分离因子均大于1 89,选择性高。该分子印迹聚合物对阿司匹林的最大表观吸附量Qmax为67 7mg/g,平衡离解常数Kd为2 33×10-4mol/L,对阿司匹林的选择性吸附可在30min内基本完成。     

人血红蛋白分子印迹聚合物的制备及分子识别性能

以丙烯酰胺和甲基丙烯酸为功能单体制备得到了具有选择性识别能力的人血红蛋白分子印迹聚合物. 该印迹聚合物的分子识别特性来自聚合物的结合基团与印迹分子的功能基团之间的氢键和静电作用,以及印迹孔穴的空间几何选择性. 研究表明,聚合单体中甲基丙烯酸的含量会影响聚合物对血红蛋白的特异结合能力及分子印迹聚合物的吸附容量和洗脱性能.     

核壳型磁性表面分子印迹聚合物的制备及对罗丹明6G吸附性能研究

以罗丹明6G为模板分子,多巴胺和3-氨基苯硼酸为功能单体和交联剂,采用表面分子印迹技术,首次制备了对罗丹明6G具有优良吸附作用的核壳型磁性表面分子印迹聚合物。应用扫描电镜、透射电镜、红外光谱等对其进行表征,通过静态、动态和选择性吸附实验,研究了该分子印迹聚合物的识别选择性和吸附容量,并建立了固相萃取-分光光度法测定实际样品中痕量罗丹明6G的新方法,检测限为2.7nmol/L,回收率为86.9%~105.0%。     

L-色氨酸分子印迹聚合物的手性识别性能研究

目的:研究L-色氨酸分子印迹聚合物的手性识别性能。方法:采用沉淀聚合法合成印迹聚合物,经洗脱后吸附已知浓度的L、D-色氨酸标准溶液,用茚三酮显色,用紫外法检测标准液被吸附前后的浓度变化,并计算出各自的吸附量。结果:模板聚合物对L-色氨酸的吸附量明显高于D-色氨酸。结论:聚合物具有良好的手性识别性能。     

分子识别和分子印迹聚合物微球

分子识别在许多分离、催化以及生物化学过程中常常起着决定性作用。例如 ,分子结构上的微小差别往往可以决定某个生化反应 (如酶反应 )是否能够顺利进行 ,所以长期以来 ,科学家们一直努力寻找分子识别的途径。二十多年前 ,Ｗｕｌｆｆ等采用一种全新的被称作分子印迹(Ｍｏｌｅｃｕｌａｒｉｍｐｒｉｎｔｉｎｇ)的技术合成出了对糖类和氨基酸衍生物有识别作用的聚合物。此聚合物被称为分子印迹聚合物 (亦称分子模板聚合物 )。分子印迹技术就是指以特定的分子为模板 ,制备对该分子有特殊识别功能和高选择性材料的技术。该技术在最近几年内发展极…     

分子印迹聚合物材料的制备及其应用

作为一种人工受体合成技术,分子印迹方法近年来取得了长足的进展。本文阐述了分子印迹方法的基本原理,详细地讨论了单体、交联剂和印迹分子的选择以及材料的制备等问题。通过讨论,分析了共价及非共价两种结合模式,指出了两者各自的适用范围;比较了3种不同印迹聚合物材料的制备方法;详细介绍了分子印迹聚合物材料在色谱分离、传感器技术、有机合成、催化材料及环境保护等各个领域的具体应用。     

表面分子印迹聚合物载体研究新进展

简述了表面分子印迹聚合物载体研究的最新进展。根据载体种类以及表面修饰方法的不同,分别介绍了以无机材料为载体和以壳聚糖为基质的表面分子印迹技术,并对表面分子印迹聚合物载体的发展前景进行了展望。对无机材料的表面修饰主要通过引入烷基化试剂的功能基团（如氨基、苄基等）,再通过分子印迹的方法制备出理想的表面分子印迹聚合物;而对壳聚糖的修饰主要通过各种交联方法,从而获得单一基质载体或者壳聚糖杂化材料载体。文中指出,与传统方法相比,新型材料的吸附性能优良、回收利用率高、颗粒均一。但是,该材料制备过程中的微观行为模式以及功能单体同目标分子的成键规律等还有待进一步研究。     

D-对羟基苯甘氨酸分子印迹聚合物的制备及分子识别能力

以D-对羟基苯甘氨酸（D-HPG）为模板分子,以丙烯酰胺（AM）和α-甲基丙烯酸（MAA）为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成了D-HPG分子印迹聚合物,并测定其对D-HPG的识别能力。静态吸附实验表明,以MAA为功能单体制备的分子印迹聚合物具有更强的分子识别能力,其饱和吸附量达43.9 μmol•g^-1,印迹因子α为3.6。分子力学计算表明,MAA与D-HPG形成复合物的结合能较大,且MAA在乙醇和水中的溶剂化能较小,因此与AM比较,MAA和模板分子D-HPG在乙醇和水中能形成更加稳定的复合物。紫外光谱分析表明,MAA与D-HPG之间的结合力比AM与D-HPG之间的结合力要强,与分子力学计算以及静态吸附实验得到的结果相一致。     

杀铃脲分子印迹聚合物制备及识别性能的研究

采用分子印迹技术,以杀铃脲为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,在四氢呋喃溶剂中制备了杀铃脲分子印迹聚合物。以平衡吸附及高效液相色谱的方法对聚合物进行了评价。印迹聚合物对模板分子有较强的吸附能力和良好的选择性,可以很好的分离模板分子及其结构类似物。     

Preparation of molecularly imprinted polymer for sinomenine and study on its molecular recognition mechanism

A sinomenine (SIN) molecule-imprinted monolithic stationary phase (MIMSP) with specific recognition for SIN was prepared by in situ technique, utilizing methacrylic acid (MAA) as a function monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linking agent, and low-polar solvents (toluene and dodecanol) as porogenic solvents. The selectivity of the polymers for SIN was evaluated by high performance liquid chromatography (HPLC). Some chromatographic conditions, such as the column temperature, the flow rate and the composition of the mobile phase, were changed in order to characterize the chromatographic procedure. SIN could be separated from some other structural analogues, including morphine, codeine, codethyline and magnoflorine, under optimized conditions. Scatchard analysis showed that two classes of binding sites existed in the SIN-imprinted polymers, with their dissociation constants estimated to be 7.257×10⁻⁵ and 3.828×10⁻³ mol l⁻¹, respectively. Compared with the SIN-imprinted polymers, the non-imprinted polymers prepared using the same method but in the absence of SIN did not exhibit the specific molecular selectivity, which suggests that the specific molecule-recognition ability of the SIN-imprinted polymers was largely ascribed to the imprinting effect.     

莱克多巴胺分子印迹聚合物的制备及吸附性能研究

采用沉淀聚合法制备莱克多巴胺分子印迹聚合物,并通过平衡吸附实验和Scatchard分析,考察其对模板分子莱克多巴胺的吸附性能和识别能力。结果表明,所制备的分子印迹聚合物对莱克多巴胺具有良好的识别能力和吸附性。     

焦性没食子酸分子印迹聚合物的制备与吸附特征研究

以焦性没食子酸为模板分子,丙烯酸为功能单体,甲醇为溶剂,研究了模板分子与功能单体的结合比例,表明焦性没食子酸和丙烯酸之间通过氢键形成1∶1型配合物。在模板分子与功能单体、交联剂物质的量的比为1∶4∶20的条件下,采用沉淀聚合法制备了焦性没食子酸分子印迹聚合物微球。静态吸附实验表明,可制备出吸附量大且特异性识别能力较高的分子印迹聚合物,对焦性没食子酸有较高的亲和性和选择性。采用恒温振荡平衡吸附法以及Scatchard分析研究了聚合物的吸附特征,结果表明焦性没食子酸分子印迹聚合物在水环境下存在2种吸附位点,最大表观吸附量分别为7.5516μg/mg和11.9225μg/mg,平衡离解常数分别为9.2720×10-3mmol/L和0.1892 mmol/L。     

Precipitation polymerization of molecularly imprinted polymers for recognition of melamine molecule

Molecular imprinted polymer microspheres (MIPs) were prepared by precipitation polymerization using melamine as template molecule, methacrylic acid (MAA) as functional monomer, trimethylol‐propane trimethacrylate (TRIM) as crosslinking agent, acetonitrile as solvent and dispersion medium. Release of the template was performed by continuous extraction with methanol containing 10% acetic acid. The microspheres were observed by scanning electron microscopy (SEM). The perfect microspheres were produced when the addition of crosslinker was 7.48 mmol. The binding capacity of MIPs was examined, Q_max = 68.36 μmol g^?1, and the dissociation constant at binding site of MIPs, K_d = 0.761, was estimated. Compared with the performance of conventional imprinted polymer, the imprinted microspheres showed high selectivity in special binding to template molecule. The imprinted microspheres could be used as the stationary phase in HPLC or SPE for selective extraction of melamine in daily products. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011     

表面印迹聚合物的制备与吸附性能

以L-色氨酸作为模板分子,丙烯酰胺为功能单体,N,N'-亚甲基双丙烯酰胺为交联剂,通过在聚甲基丙烯酸甲酯多孔基质球表面复合印迹层,制备了L-色氨酸表面印迹聚合物微球(SMIPs),探索了不同比表面积基质球及添加丙烯腈对SMIPs吸附性能的影响,结果表明基质球比表面积为432.0m~2/g时,SMIPs对L-色氨酸的吸附量较高;当n(AN):n(L-trp):3:1时,SMIPs的综合吸附性能较好.     

Synthesis and optimization of molecularly imprinted polymers for quercetin

Molecularly imprinted polymers (MIPs) were synthesized through solution polymerization using quercetin as the template molecule, N‐vinylpyrrolidone and acrylic acid as functional monomers, N,N′‐methylenebiacrylamide as crosslinker and the redox system L ‐ascorbic acid and hydrogen peroxide as initiator in the porogen of ethanol and water. During the optimization process an interference compound, genistein (5,7,4′‐trihydroxy isoflavone), which possesses the same skeleton and functional groups as quercetin, was adopted to optimize the preparation conditions, and the separation degree of the MIP to quercetin and genistein was chosen to optimize each factor. The synthesized MIP under optimal conditions showed a specific recognition of quercetin from a mixture of quercetin and genistein. Thereafter, the structure of the MIP was comparatively characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and Brunauer–Emmett–Teller analysis using non‐imprinted molecular polymers as control. In addition, the kinetics of the adsorption process were also studied, and Scatchard analysis revealed that heterogeneous binding sites were formed in the polymers. Copyright © 2012 Society of Chemical Industry     

格列美脲分子印迹聚合物应用于固相萃取的研究

以降血糖药物格列美脲为模板分子,ɑ-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,采用本体聚合法制备了格列美脲分子印迹聚合物(MIP),用于血浆样品前处理,建立了对加标兔血浆中格列美脲含量的固相萃取检测方法,通过高效液相色谱法测定,分子印迹固相萃取柱的回收率可达80%以上,有效地减少了基体中蛋白质等杂质对目标物检测的干扰,适用于生物样品中模板分子的富集和纯化。     

Comparison of molecular adsorption ability of the molecularly imprinted polymers prepared by ethylene glycol dimethacrylate and trimethylolpropane trimethacrylate as cross linkers

The target of this study was to synthesize the molecularly imprinted polymers (MIPs) of L ‐phenylalanine as the solid phases for characterization of molecular adsorption by molecularly imprinted solid phase extraction (MISPE). These MIPs, in microscale, were synthesized using thermal (40°C)‐compared with thermal (65°C)‐initiated polymerization process. Itaconic acid was chosen as the functional monomers, and either ethylene glycol dimethacrylate or trimethylolpropane trimethacrylate (TRIM) was used as the cross linker and was compared together. The influences of several parameters on the properties of the MIPs were investigated, especially physical robustness from the percentage yields and molecular adsorption from the percentage recovery by MISPE. The best yields were obtained from polymers made using TRIM and thermal (65°C)‐initiated polymerization. However, there were no significant differences in molecular adsorption. It was concluded that these parameters can be considered to synthesize MIPs for chiral separation in advance steps such as other related chromatographic techniques. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 103: 2325–2330, 2007     

硅胶表面罗红霉素分子印迹聚合物的制备及选择性吸附性能

以γ-氨丙基三甲氧基硅烷(AMPS)为硅胶表面改性剂,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,罗红霉素(ROX)为模板分子,制备得到罗红霉素分子印迹硅胶材料(ROX-MIP-PMAA/SiO₂),采用红外、扫描电镜和粒径测定等方法对其进行了表征.通过静态和动态吸附实验研究了ROX-MIP-PMAA/SiO₂对罗红霉素的吸附性能,并以红霉素为竞争底物,研究其选择吸附性能.结果显示,ROX-MIP-PMAA/SiO₂对罗红霉素的吸附能力明显大于非印迹硅胶(NIP-PMAA/SiO₂),其对罗红霉素和红霉素的分离因子为1.21,说明其对罗红霉素具有较好的选择吸附性能.