共查询到20条相似文献,搜索用时 93 毫秒
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革兰阴性(Gram-negative,G-)菌的分泌系统至少有9种,依次命名为Ⅰ~Ⅸ型分泌系统,其中V型分泌系统(type V secretion system,T5SS)亦被称为自主转运分泌系统(autotransporters,AT),广泛存在于G-菌中.该系统分泌的蛋白占G-菌外泌蛋白的比例最高.该系统只含1或2... 相似文献
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简述了表面分子印迹聚合物载体研究的最新进展。根据载体种类以及表面修饰方法的不同,分别介绍了以无机材料为载体和以壳聚糖为基质的表面分子印迹技术,并对表面分子印迹聚合物载体的发展前景进行了展望。对无机材料的表面修饰主要通过引入烷基化试剂的功能基团(如氨基、苄基等),再通过分子印迹的方法制备出理想的表面分子印迹聚合物;而对壳聚糖的修饰主要通过各种交联方法,从而获得单一基质载体或者壳聚糖杂化材料载体。文中指出,与传统方法相比,新型材料的吸附性能优良、回收利用率高、颗粒均一。但是,该材料制备过程中的微观行为模式以及功能单体同目标分子的成键规律等还有待进一步研究。 相似文献
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纳米技术与纳米材料(Ⅰ)--纳米技术与纳米材料简介 总被引:18,自引:0,他引:18
介绍了纳米、纳米结构的基本概念和涵义,阐述了纳米技术的内涵及其产生、发展和前景,并对纳米技术的诞生起着先导性作用的扫描隧道显微镜、原子力显微镜的原理及应用作了简要描述。同时,对纳米科技中最为重要的研究领域--纳米材料的定义、分类和发展作了论述,并介绍了纳米材料与常规块体材料迥异的独特性能及其应用潜力,最后对如真空冷凝法、机械球磨法、气相沉积法、化学沉淀法、水热合成法、原位生成法以及溶胶-凝胶技术、模板技术及自组装技术等几种较为前沿的纳米材料的制备方法、制备原理和特点作了讨论。 相似文献
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Dietmar Pum Jose Luis Toca-Herrera Uwe B. Sleytr 《International journal of molecular sciences》2013,14(2):2484-2501
Crystalline S(urface)-layers are the most commonly observed cell surface structures in prokaryotic organisms (bacteria and archaea). S-layers are highly porous protein meshworks with unit cell sizes in the range of 3 to 30 nm, and thicknesses of ~10 nm. One of the key features of S-layer proteins is their intrinsic capability to form self-assembled mono- or double layers in solution, and at interfaces. Basic research on S-layer proteins laid foundation to make use of the unique self-assembly properties of native and, in particular, genetically functionalized S-layer protein lattices, in a broad range of applications in the life and non-life sciences. This contribution briefly summarizes the knowledge about structure, genetics, chemistry, morphogenesis, and function of S-layer proteins and pays particular attention to the self-assembly in solution, and at differently functionalized solid supports. 相似文献
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Naa Labajov Natalia Baranova Miroslav Jursek Robert Vcha Martin Loose Imrich Bark 《International journal of molecular sciences》2021,22(15)
DivIVA is a protein initially identified as a spatial regulator of cell division in the model organism Bacillus subtilis, but its homologues are present in many other Gram-positive bacteria, including Clostridia species. Besides its role as topological regulator of the Min system during bacterial cell division, DivIVA is involved in chromosome segregation during sporulation, genetic competence, and cell wall synthesis. DivIVA localizes to regions of high membrane curvature, such as the cell poles and cell division site, where it recruits distinct binding partners. Previously, it was suggested that negative curvature sensing is the main mechanism by which DivIVA binds to these specific regions. Here, we show that Clostridioides difficile DivIVA binds preferably to membranes containing negatively charged phospholipids, especially cardiolipin. Strikingly, we observed that upon binding, DivIVA modifies the lipid distribution and induces changes to lipid bilayers containing cardiolipin. Our observations indicate that DivIVA might play a more complex and so far unknown active role during the formation of the cell division septal membrane. 相似文献
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Dr. Eszter Csibra Dr. Marleen Renders Prof. Dr. Vitor B. Pinheiro 《Chembiochem : a European journal of chemical biology》2020,21(19):2844-2853
Directed evolution has been remarkably successful at expanding the chemical and functional boundaries of biology. That progress is heavily dependent on the robustness and flexibility of the available selection platforms, given the significant cost to (re)develop a given platform to target a new desired function. Bacterial cell display has a significant track record as a viable strategy for the engineering of mesophilic enzymes, as enzyme activity can be probed directly and free from interference from the cellular milieu, but its adoption has lagged behind other display-based methods. Herein, we report the development of SNAP as a quantitative reporter for bacterial cell display, which enables fast troubleshooting and the systematic development of the display-based selection platform, thus improving its robustness. In addition, we demonstrate that even weak interactions between displayed proteins and nucleic acids can be harnessed for the specific labelling of bacterial cells, allowing functional characterisation of DNA binding proteins and enzymes, thus making it a highly flexible platform for these biochemical functions. Together, this establishes bacterial display as a robust and flexible platform, ideally suited for the systematic engineering of ligands and enzymes needed for XNA molecular biology. 相似文献
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