酶工程研究及应用

酶工程是现代生物技术的重要组成部分，它作为一项高新技术将为各工业的发展起重要推动作用。介绍了酶固定化、基因工程菌（细胞）的固定化、植物细胞培养产酶、酶的化学修饰、核酸酶、抗体酶、酶标药物的理论和技术研究的最新进展以及酶工程在各工业中的应用，对酶工程的发展前景进行了探讨。     

酶工程研究的新进展

介绍了自然酶的开发、酶的化学和遗传修饰、酶的固定化、人工合成酶、酶基因的克隆和表达、酶的遗传设计等方面的理论和技术研究的最新进展。     

酶工程的研究进展

酶工程是现代生物技术的重要组成部分,它作为一项高新技术将为工业的发展起重要推动作用.介绍了自然酶的开发、酶的化学和遗传修饰、酶的固定化、人工合成酶、酶基因的克隆和表达、酶的遗传设计等方面的理论和技术研究的最新进展.     

酶工程在医药工业中的应用

酶工程是现代生物技术的重要组成部分,它作为一项高新技术将为各工业的发展起重要推动作用。介绍了酶固定化、基因工程菌(细胞)的固定化、植物细胞培养产酶、酶的化学修饰、核酸酶、抗体酶、酶标药物的理论和技术研究的最新进展以及酶工程在医药工业中的应用,对酶工程的发展前景进行了探讨。     

分离技术的进展

本文概述了基于生物技术的发展、超纯材料的制备等对发展分离科学和技术的需求,特别是从大量基体物或稀溶液中提取、分离、纯化和检测低含量物质要求发展高选择性的分离技术和相应的分离体系。在发展和完善原有分离技术(如萃取、离子交换、吸附、吸收等)的同时,超临界萃取和膜分离技术具有引入的应用前景。还应注意两种或多种分离技术相结合的分离方法和技术的研究。     

二氯苯生产技术进展与发展趋势

介绍了二氯苯生产方法，用途，市场需求及发展趋势，重点介绍了二氯苯异构体分离技术，针对国内对，邻二氯苯生产现状，提出一些发展建议。     

国外芳烃生产技术进展

阐述了国外芳烃生产技术在以下方面的进展：催化重整,轻烃转化为芳烃,芳烃间的转化,芳烃抽提。     

精馏技术进展与设计

本文主要介绍国内外近几年精馏设备的发展动向，同时介绍了新精馏方法和新精馏操作方法，并对精馏设计的发展方向做了论述。     

中药制剂分离与纯化技术创新进展

论述了深入研究中药制剂分离技术创新进展的意义,概述了固液分离技术在中药制剂中的重要作用,首次全面总结了14种制药分离与纯化主要设备的重大发展历程及创新特征,首次系统剖析了14种中药制剂提取分离与纯化高新技术原理特性和应用效果,首次研究阐明了现代中药制剂分离与纯化技术主要呈现的新老技术复合化、高技术超常化和新技术多样化三大发展趋势。     

油水重力分离设备技术及进展

结合作者工作实践,从油水分离技术工业设备应用的现状和生力分离设备的发展概况等方面,介绍了油水重力分离设备的发展过程。     

生物工程酶在化工中的应用进展

阐述了酶应用的历史起源及酶在化工中应用的发展过程,介绍了酶在化学工业中的覆盖面、地位和现状以及生物工程酶与化工融合发展的潜力和趋势.     

我国生物工程发展的现状与展望

我国在８０年代初即将生物工程确定为科技和产业发展的重要领域，制订了发展的战略目标和具体规划，组织了攻关队伍，采取各种措施，使我国生物工程的发展走上了有计划的迅速发展道路，取得了许多重大的突破性进展，为在本世纪末形成一定规模的生物工程新产业，跻身世界生物工程先进行列，打下了坚实基础。     

胶原与假体生物工程材料的对接研究进展

目录导言1什么是假体生物工程材料？2假体生物工程材料制备方法2．1金属粉末焙烧2．2造孔剂的应用2．3电场活性固化2．4复制方法     

生物工程技术的发展及其在石油化工中的应用

阐述了现代生物工程及其应用领域 ,论述了生物工程的现状及其发展趋势 ,展示了生物工程在石油化工领域的应用前景。     

酶防污涂料的探索及其发展前景

用酶防污,消除毒素对环境的危害,这是环境友好型防污涂料的重要发展方向之一。阐述了酶防污涂料的发展历程、防污机理以及就"留住"防污涂料中的酶及其发展前景作了粗浅的探索。     

Bioengineering of glass surface

The possibility of implementing a new scientific field—bioengineering of glass surface—was considered. The growth of different diatom algal cultures and the density of their immobilization on the surface of glass were studied. The experimental results that confirm the possibility of the bioengineering formation of glass surface micro- and nanostructures using diatom algae were represented.     

靶标酶在农药研发中的作用

靶标酶在农药研发中具有极其重要的作用,大多数新开发的超高活性农药的靶标都是各种重要的代谢酶系统,了解靶标酶的作用机理,将有助于开发新型高效、低毒、专一性好的农药.综述了除草剂和杀虫剂作用于靶标酶的机理、针对专一靶标酶开发的除草剂和杀虫剂品种、昆虫和杂草产生抗药性的机制,并对新型除草剂和杀虫剂的开发前景进行了展望.     

Bioengineering of hepatitis B vaccines

It has been said that ‘never in the history of human progress has a better and cheaper method of preventing illness been developed than immunisation’. This is well illustrated by the WHO Expanded Programme on Immunisation (EPI), which in developing countries is now preventing nearly a million deaths annually from measles, pertussis, and neonatal tetanus, and for which there is a commitment by the WHO and UNICEF to protect all children by immunisation by the end of the decade. This enormous undertaking will be facilitated by the rapid advances in molecular biology and recombinant DNA technology, in the understanding of immunological mechanisms and by the production and application of monoclonal antibodies so that the structure and location of important antigenic determinants or epitopes can be determined. Chemical synthesis of oligopeptides has been simplified, and computer programmes and X-ray crystallography provide the tools for the determination of three-dimensional structure of proteins, so that the structure and location of important antigenic determinants or epitopes can be predicted. These techniques have opened the way to the improvement of existing vaccines and to the development and production of new vaccines against infections for which vaccines are not available. New vaccines under development include vaccines against hepatitis B, hepatitis A, malaria, vaccines for typhoid, cholera, rotavirus infection and other diarrhoeal diseases, leprosy, rabies, fertility regulating vaccines and the acquired immune deficiency syndrome (AIDS).     

Development of an Enzyme Mimic Using Self-Selection

The development of a serine protease model using a self-selection protocol is described. The developed system mimics the regeneration step of an enzyme involved in covalent enzyme catalysis. A transition-state analogue of a transesterification reaction is used to self-select functional groups able to accelerate ester cleavage. It is shown that the insertion of a tertiary amine substituent flanking the reaction center reinforces transition-state stabilization by directing the reactive center towards the self-selected functionality. In addition, the tertiary amine activates a bland (solvent) nucleophile for attack on an ester bond similar to what occurs in a serine protease. A quantitative correspondence is observed between the amplification factors and catalytic activity, illustrating the potential of the dynamic covalent capture strategy to precisely detect and quantify weak noncovalent interactions.     

Bioengineering of Anti-Inflammatory Natural Products

Inflammatory processes occur as a generic response of the immune system and can be triggered by various factors, such as infection with pathogenic microorganisms or damaged tissue. Due to the complexity of the inflammation process and its role in common diseases like asthma, cancer, skin disorders or Alzheimer's disease, anti-inflammatory drugs are of high pharmaceutical interest. Nature is a rich source for compounds with anti-inflammatory properties. Several studies have focused on the structural optimization of natural products to improve their pharmacological properties. As derivatization through total synthesis is often laborious with low yields and limited stereoselectivity, the use of biosynthetic, enzyme-driven reactions is an attractive alternative for synthesizing and modifying complex bioactive molecules. In this minireview, we present an outline of the biotechnological methods used to derivatize anti-inflammatory natural products, including precursor-directed biosynthesis, mutasynthesis, combinatorial biosynthesis, as well as whole-cell and in vitro biotransformation.