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影响风电机组使用性能的重要因素是风电机组故障率,大型风电机组在我国发展时间不长,人们对于机组零部件、主要系统产生故障的规律和特征还缺少深入研究和经验积累。现分析了风电机组设备维护维修策略,并对机组各类故障进行了统计,以期能为风电机组关键部件的故障诊断和运行状态监测提供理论依据。 相似文献
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福建晋江天然气发电有限公司电厂4台机组为美国GE公司生产的S109FA燃气蒸汽联合循环机组,其中汽轮机型号为#158(D10优化型),为三压、一次中间再热、单轴、双缸双排汽、冲动式无抽汽纯凝式机组。由于机组常用于电网调峰,故启动次数较多,经常出现机组振动异常事件。为此,对机组振动异常事件进行了分析对比,提出了运行改进措施,为解决同类型机组的振动异常问题提供了参考。 相似文献
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通过对机组在线监测数据、现场实测数据和机组运行情况综合分析得出机组振动波动的主要原因,为检维修决策提供依据,保障了机组的快速修复。 相似文献
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1 000 MW超超临界机组系统复杂,每次启动消耗都很大。现以某厂1 000 MW机组为例,详细分析了影响该机组启动时间的因素,探讨了机组冷态启动方式的优化措施,并对优化前后的效果进行了对比,结果显示,优化机组启动方式在节省厂用电、燃油、燃煤、水、辅汽等方面经济效益显著。 相似文献
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Cadmium, zinc, selenium, and copper were administered, singly or in combination, orally or subcutaneously. Experiment I included 32 calves of both sexes; six received Cd (two groups), Zn, Cd, and Zn, and Cd and Se (two groups) and one group was a control. In Experiment II (21 bulls), three were given Cd, Cd, and Cu, and Cd and Zn, respectively, and one group was a control. For light microscopy, in Experiment I the highest amounts of silver granules were present in the samples of liver, small intestine, and vesicular gland of all the exposed groups; in Experiment II the most affected organs were liver, kidney, and small intestine. For electron microscopy, in Experiment I, after administration of Cd and Zn, the highest amounts of granules were seen in the cytoplasm of hepatocytes and cells of the proximal and distal renal tubules and the lowest amounts were found in glandular cells of the pancreas. Administration of Cd and Se resulted in the presence of large numbers of granules in the nuclei and nucleoli of spermatogonies. In Experiment II, ingestion of Cd and Zn in feed led to the appearance of highest amounts of granules in the nucleoli, nuclei, and cytoplasm of cells in testes, kidneys, and pancreas. Following Cd intake, the highest accumulation of granules was observed in the nucleoli of hepatocytes and cells of the proximal and distal renal tubules. Combined Cd and Cu produced the highest number of granules in cells of the proximal and distal renal tubules and in the nucleoli and nuclei of germinal epithelium. 相似文献
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In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind. Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes. This approach analyzes the final products of cellular regulatory processes, the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes. Microbial metabolomics has been widely used in different research fields, such as microbial phenotypic classification, mutant screening, metabolic pathways, microbial metabolic engineering, fermentation engineering monitoring and optimization, microbial environmental pollution, and so on. However, there are only a few reviews on microbial metabolomics of probiotics and pathogens. This review summarizes the main methodologies, including sample preparation, identification of metabolites, data processing, and analysis. Recent applications in microbial metabolomics of probiotics and pathogens are also described. This paper first summarized the research progress and application of microbial metabolomics from two aspects: probiotics and pathogenic bacteria. Probiotics and pathogenic bacteria do not exist independently most of the time; hence, these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria, which was subdivided into important microbial research fields closely related to human health, including the human gut, oral cavity, food, and nutrition-related microorganisms. Then, the main problems and trends associated with microbial metabolomics are discussed. 相似文献
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智能机床成为机床装备发展的趋势。在介绍国内外智能机床的研究现状的基础上,分析目前智能机床技术研究的三个问题,提出智能机床的狭义和广义定义;给出智能机床的包括人计机的协同性、整体与局部的协调性、智能的恰当性与无止性、自学习及其能力持续提高性、自治与集中的统一性、结构的开放性和可扩展性、制造和加工的绿色性、智能的贯穿性八个智能技术特征;阐述包括以人为中心的人计机动态交互功能、三个基本功能——执行智能功能、准备智能功能、维护智能功能的智能功能特征,以及功能之间的相互关系;建立八个理论与技术的智能机床技术理论框架,包括人计机一体化融合理论与技术、多源信息的感知理论与技术、智能决策理论与技术、智能执行理论与技术、智能维护理论与技术、智能机床综合能力评价理论与技术、智能机床标准化技术、智能机床支撑技术等,形成了智能机床的技术体系框架,同时指出上述内容将随着技术的进步和应用的深入不断充实和丰富。 相似文献
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Although the occurrence of microbial (algal, protozoan, bacterial, and fungal) epibionts on marine crustaceans and other invertebrates has been documented repeatedly, the ecological context and significance of these relationships generally are not well understood. Recently, several studies have examined the population and community ecology of algal and protozoan epibionts on freshwater crustaceans. Even so, the study of microbial epibionts in aquatic environments is still in its infancy. In this review, we summarize associations of microalgae, protozoans, and bacteria with marine crustaceans, especially copepods. We note differences and commonalities across epibiont taxa, consider host-epibiont cycling of nutrients, generate hypotheses relevant to the ecology of the host and the epibiont, and suggest future research opportunities. Microsc. Res. Tech. 37:116–135, 1997. © 1997 Wiley-Liss, Inc. 相似文献
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Semiautomated methods are used to measure elongated, curved and complex branching profiles and isolated perimeter segments in monochrome video images with a general-purpose analysis system. These methods are used to make the major primary measurements of bone histomorphometry. Accuracy and reproducibility of the image acquisition, processing and measurement system is documented by measuring a semicircular standard of known dimensions. Semiautomated applications of the Ar/Le method for measuring areas and perimeters, and calculating lengths and widths of osteoid seams, lengths of mineralization labels and mineral apposition rate, wall width, indirect measurements of eroded, osteoclastic and osteoblastic perimeters without tracing, and measurement of mineralized or total cancellous bone area and perimeter gave values comparable to measurements of the same parameters by tracing or grid counting techniques with equal or better reproducibility and much greater efficiency. Intraindividual variation in measuring multiple bone biopsies was comparable to that reported with current standard methods. Major sources of variability for semiautomated methods were image magnification and selection of profile edges by thresholding, and sources of variability for manual methods are image magnification, numbers of orthogonal intercepts, tracing speed and accuracy of the algorithm used to measure traced pixels. Semiautomated methods are accurate, reproducible and rapid methods suitable for bone histomorphometry. 相似文献
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Xiuchun ZHANG 《Biocell》2019,43(4):313-319
To investigate the effects of polydatin on the proliferation, migration, and invasion of ovarian cancer, the
change of proliferative ability, migration ability, and invasive ability of human ovarian cancer cell OVCAR-3, A2780,
and HO-8910 was detected by using polydatin and up-regulating PI3K. The anticancer activity and mechanism of
polydatin in ovarian cancer were analyzed. Polydatin could effectively inhibit the proliferation, migration, and invasion
of OVCAR-3, A2780, and HO-8910, and inhibit the expression of PI3K protein. After the expression level of PI3K
protein was up-regulated, the inhibitory effect of polydatin on the proliferative ability, migration ability, and invasive
ability of OVCAR-3, A2780, and HO-8910 significantly decreased, suggesting that PI3K was the target of polydatin.
Therefore, we concluded that polydatin could inhibit the proliferation, migration, and invasion of ovarian cancer cells
by inhibiting the expression of PI3K protein, which provides an experimental basis for polydatin in the treatment of
ovarian cancer. 相似文献
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Shafqat Ali Khan Ghulam Dastagir Noor Ul Uza Akhtar Muhammad Rahim Ullah 《Microscopy research and technique》2020,83(6):636-646
The present study was conducted on characterization of morpho‐anatomical, phytochemical, and bio‐elemental analysis of root, stem, and leaf of Verbascum thapsus. Morphologically Verbascum is a biennial plant that flowers for a month and a half in mid‐ to late summer. Various organoleptic features of root, leaf, and stem were recorded. Anatomically the T. S of the root, stem, and leaf showed a typical dicot histological differentiation. Leaf possessed anomocytic stomata, crescent shape vascular bundles, and covered with long and stellate type trichomes while, stem contained collateral type of vascular bundles and a well‐developed pith to store phytochemicals responsible for various pharmacological activities. The powder drug study through scanning electron microscopy revealed the presence of various types of tissues. Branched, tree like and stellate trichomes in root and leaf help in absorption and reduce loss of water. These anatomical features are responsible for the survival of the plant as biennial. Four macro elements (Na, K, Ca, and Mg) and seven microelements (Cr, Cu, Fe, Mn, Ni, Zn, and Cd) and their concentrations in ppm were also studied using Atomic Absorption Spectroscopy. Phytochemical screening of methanolic extract showed existence of various secondary metabolites, while mucilage and anthraquinones was not detected. The present study helps to understand the taxonomic identification of the plant based on morpho‐anatomical features and throws the attention of the researchers to carry out the work for developing its various formulations, which can ultimately be beneficial for the human beings as well as animals. 相似文献
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在长期进化过程中,自然界中的多种动物、植物形成了独特的轻质、高强结构,以此来抵抗外界的复杂冲击载荷,保护自身完整,满足生存需要。生物轻质高强结构的优越性,启发了科研和工程人员采用结构仿生学的方法来对管状和板状两大类吸能结构进行设计优化和改进。对竹子、茎秆/树干、羽轴、骨骼四类管状生物结构和甲虫鞘翅、贝壳、柚子皮、龟壳四类板状生物结构进行综述,阐述了分层、多孔、螺旋、中空等多种结构与轻质高强特性之间的关系。在此基础上,对比和分析了相应的结构元素在单胞管、多胞管、嵌套管、波纹管等管状吸能结构和蜂窝夹芯板、复合材料板、混合结构板等板状吸能机构中起到的作用。进一步对当前仿生吸能领域存在的结构复杂、质量大、缺乏普适性的机理和过渡“桥梁”等问题做出了分析;最后对仿生吸能技术的形式简单化、结构轻量化、理论通用化、“形神兼备”化发展趋势做出展望。 相似文献
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Y Wakayama M Inoue H Kojima M Murahashi S Shibuya H Oniki 《Microscopy research and technique》2001,55(3):154-163
In order to investigate the mode of existence of the sarcoglycan complex, neuronal nitric oxide synthase (nNOS), beta-dystroglycan, and dystrophin in the normal skeletal myofiber, we examined the ultrastructural localization and mutual spatial relationship of nNOS, beta-dystroglycan, dystrophin, and the individual components of the sarcoglycan complex by using triple immunogold labeling electron microscopy. Each molecule of alpha-, beta-, gamma- and delta-sarcoglycans is located intracellularly or extracellularly near the muscle plasma membrane mostly in accordance with the sarcoglycan antigenic sites against which the antibodies were generated. The association of different two and/or three sarcoglycan molecules out of alpha-, beta-, gamma- and delta-sarcoglycan molecules was frequently observed. Each molecule of nNOS, beta-dystroglycan, and dystrophin was ultrastructurally noted along the cell surface of normal skeletal myofibers. Moreover, the close relation of a sarcoglycan molecule with beta-dystroglycan and dystrophin, and the association of nNOS with dystrophin were also confirmed ultrastructurally. Thus, this study demonstrated that the constituting molecules of the sarcoglycan complex, nNOS, beta-dystroglycan, and dystrophin existed in the form of a cluster at the normal muscle plasma membrane. The association of nNOS with dystrophin and its associated glycoproteins may form a macromolecular signaling complex at the muscle plasma membrane. 相似文献
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Salvadori ML Lessa TB Russo FB Fernandes RA Kfoury JR Braga PC Miglino MA 《Microscopy research and technique》2012,75(10):1437-1444
In this work, we studied the embryology of mice of 12, 14, and 18 days of gestation by gross observation, light microscopy, and scanning electron microscopy. Grossly, the embryos of 12 days were observed in C‐shaped region of the brain, eye pigmentation of the retina, first, second, and third pharyngeal arches gill pit nasal region on the fourth ventricle brain, cervical curvature, heart, liver, limb bud thoracic, spinal cord, tail, umbilical cord, and place of the mesonephric ridge. Microscopically, the liver, cardiovascular system and spinal cord were observed. In the embryo of 14 days, we observed structures that make up the liver and heart. At 18 days of gestation fetuses, it was noted the presence of eyes, mouth, and nose in the cephalic region, chest and pelvic region with the presence of well‐developed limbs, umbilical cord, and placenta. Scanning electron microscopy in 18 days of gestation fetuses evidenced head, eyes closed eyelids, nose, vibrissae, forelimb, heart, lung, kidney, liver, small bowel, diaphragm, and part of the spine. The results obtained in this work describe the internal and external morphology of mice, provided by an integration of techniques and review of the morphological knowledge of the embryonic development of this species, as this animal is of great importance to scientific studies. Microsc. Res. Tech. 2012. © 2012 Wiley Periodicals, Inc. 相似文献