The effect of cooking methods on mineral and vitamin contents of African catfish

Proximate composition, mineral and vitamin contents (A, E, B₁, B₂, niacin and B₆) were investigated in cooked African catfish. Different cooking treatments were used (baking, grilling, microwaving and frying). The protein and ash contents increased in all cooked fish. The fat content increased only in fried fillets. The moisture content of cooked fish decreased. Mineral levels were affected by cooking methods, except for Cu Although the vitamin A content significantly increased in grilled and fried fish, vitamin E increased in all cooked fish. Vitamin B₁ content of cooked fish significantly decreased. Vitamin B₂ and niacin contents of grilled fish increased significantly. B₆ content of cooked fish significantly decreased but this did not occur for the grilled fish. High levels of vitamin and mineral contents were found in grilled catfish.     

Iodine content of cooked and processed fish in Turkey

This study evaluates the effects of different cooking (grilling, frying and steaming) and processing (smoking, salting, marinating and drying) methods on iodine contents of valuable marine and freshwater fish species commonly consumed in Turkey. The highest mean iodine content of cooked fish was determined to be 3.760 mg kg^?1, 2.788 mg kg^?1, 5.831 mg kg^?1 and 6.161 mg kg^?1 in grilled anchovy, horse mackerel, Atlantic Bonito and whiting, 0.914 mg kg^?1, 1.452 mg kg^?1, 3.106 mg kg^?1 in steamed bluefish, red mullet. The highest mean iodine content of processed fish was determined to be 2.149 mg kg^?1 in smoked mackerel, 0.701 mg kg^?1 in salted Bonito, 1.128 mg kg^?1 in dried horse mackerel and 7.283 mg kg^?1 in marinated anchovy. This iodine values are also above the Upper Tolerable Nutrient Level of 100–150 μg day^?1 iodine.     

Selenium Content of Raw and Cooked Marine Species Consumed in Portugal

The present study evaluates the effects of different cooking methods (grilling, frying and boiling) on selenium contents of six marine species commonly consumed in Portugal. Forty-two composite samples of sardine, horse mackerel, gilthead seabream, silver scabbardfish, hake and octopus were digested in a microwave system and analysed by electrothermal atomic absorption spectrometry. The described method is adequate for the analysis of selenium in marine species and meets the requirements of validation and quality control. Mean selenium contents in raw species ranged from 0.35 mg kg⁻¹ to 1.24 mg kg⁻¹. Cooked samples presented mean selenium contents from 0.38 mg kg⁻¹ to 1.85 mg kg⁻¹ in grilled fish, from 1.22 mg kg⁻¹ to 1.28 mg kg⁻¹ in fried fish and from 0.84 mg kg⁻¹ to 0.87 mg kg⁻¹ in boiled fish. No statistically significant differences were determined for selenium levels in raw and cooked samples and in different marine species. Estimated selenium intake agrees well with recommendations and is far below the Upper Tolerable Nutrient Level. This is the first study concerning the evaluation of the effects of cooking methods on selenium contents of marine species consumed in Portugal.     

Effect of Cooking Method,Distiller's Grains,and Vitamin E Supplementation on the Vitamin Content of Value Cuts from Beef Steers Fed Wet Distiller's Grains and Solubles and Supplemental Vitamin E

ABSTRACT: Vitamin E, thiamin, riboflavin, niacin, vitamin B₆, and vitamin B₁₂ concentrations of flat iron steaks and petite tenders from steers fed finishing rations containing 0% and 40% corn wet distiller's grains and solubles (WDGS) with and without supplemental vitamin E were determined. Feeding treatment groups were: 0% WDGS with basal vitamin E, 0% WDGS with supplemental vitamin E (500 IU daily), 40% WDGS with basal vitamin E, and 40% WDGS and supplemental vitamin E. Cattle can be fed 40% WDGS diets more economically than corn diets. The incorporation of 40% WDGS, with and without vitamin E, was hypothesized to have little effect on the vitamin concentrations of these value meat cuts. Flat iron steaks and petite tenders were broiled and/or grilled to 70 °C internal temperature. Mean cooking yields ranged from 68.7% to 78.2%. The majority of the vitamin concentrations of broiled and of grilled meat were significantly different (P < 0.05) from that of raw meat. Vitamin E concentrations of raw and cooked meat from steers that received supplemental vitamin E were significantly higher (P < 0.05) than those fed basal vitamin E. Significant differences in thiamin, riboflavin, vitamin B₆, and vitamin B₁₂ concentrations in raw flat iron steaks and in vitamin B₆ in raw petite tenders were observed by WDGS. Thiamin, vitamin B₆, and vitamin B₁₂ concentrations of broiled flat iron steaks were significantly different (P < 0.05) than grilled. A few differences in vitamin concentrations of the flat iron steaks and petite tenders were observed by WDGS, vitamin E supplementation, and cooking treatments, but most of the vitamin concentrations were statistically similar.     

Effects of different cooking methods on the mineral contents of wild and farmed sea bream (Sparus aurata)

The effects of different cooking methods (steaming, boiling, oven cooking, grilling, frying in olive oil, frying in soybean oil, frying in corn oil and frying in sunflower oil) on mineral contents (Ca, Mg, K, Na, Fe, Zn, Cu and Mn) of wild and farmed sea bream (Sparus aurata) were determined. The moisture rate decreased significantly (P < 0.05) after boiling, grilling, oven cooking and frying but remains stable after steaming. The fat content increased with different frying treatments. The Ca, Mg, Na, K and Zn contents of wild and farmed sea bream cooked by almost all methods significantly decreased (P < 0.05). The Cu content in grilled wild and farmed sea bream increased significantly (P < 0.05). Losses of mineral content in fried sea bream were higher than those of fish cooked by other methods. On comparing the raw and cooked fish, the results indicated that cooking had considerable effect on the mineral contents.     

Effect of sex, maturation stage and cooking methods on the nutritional quality and safety of black scabbard fish (Aphanopus carbo Lowe, 1839)

BACKGROUND: Black scabbard fish ( Aphanopus carbo Lowe, 1839) is a deep‐water fish resource that is highly appreciated in southern European countries and can accumulate high levels of mercury in the muscle. Currently, European legislation establishes limits for the presence of toxic contaminants in raw seafood, despite these products are generally cooked before consumption. In addition, there is still a lack of information concerning the nutritional quality and contaminants available in cooked products. Therefore, the aim of this study was to assess the effect of sex, maturation stages and three common cooking practices (steaming, grilling and frying) on the toxic elements (Hg, As, Cd and Pb) and nutritional value (chemical, elemental and fatty acid composition) of black scabbard fish. RESULTS: Few variations occurred between sexes and maturation stages, particularly in fatty acid and elemental content. Concerning cooked black scabbard fish, the greatest differences occurred in fried and grilled samples, attaining higher Hg levels, whereas steamed fish composition was closer to raw black scabbard fish. CONCLUSION: Raw and cooked black scabbard fish can be considered as a very good source of essential nutrients such as n‐3 PUFA, proteins, macro and trace elements. Yet, when the fish is grilled, the Hg content may be above the limits set by EU. Considering the alterations occurred during the cooking processes, steaming seems the best procedure to cook this species. Copyright © 2011 Society of Chemical Industry     

Milk Drink Using Whey Butter Cheese (queijo manteiga) and Acerola Juice as a Potential Source of Vitamin C

The production of a beverage from whey butter cheese (WBC) and acerola juice (AJ) presents good commercialization potential, uniting the benefits provided by the former with those of latter, including the ingestion of essential amino acids and increasing the vitamin C content, resulting in a product of differentiated nutritive value. Three beverage formulations were developed: B₁ (50% WBC–50% AJ v/v), B₂ (70% WBC–30% AJ, v/v), and B₃ (30% WBC–70% AJ, v/v). Lower protein and carbohydrate values were found for the formulations with less whey (p < 0.05). All the beverages presented an energy value below those declared for dairy beverages commercialized on the Brazilian market. The beverages B₁ and B₂ were considered food products that were sources of vitamin C. B₂ presented significant variations for all the sensory attributes evaluated (p < 0.05), although the results were similar to those obtained for B₁, suggesting the commercialization potential of these formulations.     

Vitamin B12 content in raw and cooked beef

The aim of this work was to evaluate the vitamin B₁₂ content and profile of cobalamin bioactive forms in raw and cooked beef. The study showed that vitamin B₁₂ distribution is unequal among beef muscles. The content of this nutrient ranges from 0.7 to 1.5 μg/100 g of tissue. In beef, three biologically active forms of vitamin B₁₂ were determined, i.e.: methylcobalamin, adenosylcobalamin and hydroxycobalamin; the dominating form of vitamin B₁₂ was adenosylcobalamin (68% of total cobalamins). The process of roasting and grilling had little effect on the vitamin B₁₂ content in the final product as compared to the raw meat. The fried product was characterised by about a 32% lower content of cobalamins than in raw meat.     

Comparison of the effects of four cooking methods on fatty acid profiles and nutritional composition of red mullet (Mullus barbatus) muscle

This study was performed to examine the effects of different cooking methods (frying, steaming, oven cooking, and microwave cooking) on proximate chemical composition, mineral contents, and fatty acids profiles of red mullet (Mullus barbatus) fillets. The frying process caused a significant increase in fat content (from 13.37±0.04 to 34.44±0.20 g/100 g d.w.) and reduced the proportion of the palmitic acid of 50.73% and saturated fatty acids of 56.9%. While, the proportion of polyunsaturated fatty acids (PUFA) increased from 4.04% of total fatty acids for raw fillet to 49.17% of total fatty acids for fried fillets. The steamed, oven-cooked, and microwave-cooked fillets showed the best proportion of fat content, PUFA, and ω-6/ω-3 ratio as compared with fried red mullet fillets. Na, Mg, and Zn contents of cooked fish fillets significantly decreased.     

Effect of chloride on the formation of 3-monochloro-1,2-propanediol fatty acid diesters and glycidol fatty acid esters in fish,meats and acylglycerols during heating

The effects of the presence of chloride on the formation of 3-monochloro-1,2-propanediol fatty acid esters (3-MCPDEs) and glycidol fatty acid esters (GEs) in saltwater fish, meats and acylglycerols (diacylglycerol and triacylglycerol) during heating were investigated in this study. Five saltwater fish species (salmon, saury, yellowtail, mackerel and Spanish mackerel) were grilled with a fish griller. 3-MCPDEs and GEs were detected in all of the grilled fish samples. The total amount of GEs was higher than 3-MCPDEs. Beef and pork patties with or without sodium chloride (1.5%) were cooked using gaseous fuel. The formation of 3-MCPDEs was significantly increased by the addition of sodium chloride to the meat patties, whereas the concentration of GEs in the cooked meat patties was not changed by the content of sodium chloride. Hexadecane solutions of diacylglycerol or triacylglycerol containing FeCl₃ were heated at 240°C. The formation of 3-MCPDEs was greatly increased by adding FeCl₃ to the solutions of triacylglycerol. The amounts of 3-MCPDEs decreased with the extension of the heating time. From these results, it is suggested that 3-MCPDEs and GEs are formed in saltwater fish and meats by cooking, and that the formation of 3-MCPDEs was affected by chloride in foodstuffs.     

Effect of different cooking methods on vitamins,minerals and nutritional quality indices of orange-spotted grouper (<Emphasis Type="Italic">Epinephelus coioides</Emphasis>)

This study aimed to evaluate the nutritional value [proximate, fatty acid profiles, vitamins and minerals and also nutritional quality indices (NQI)] of orange-spotted grouper (Epinephelus coioides) prepared according to common consumer techniques: raw, poached, steamed, microwaved, pan-fried and deep-fried (in olive oil). There was an increase in protein, lipid and ash contents in cooked fish as compared to raw fish fillets. Cooking treatment had no significant effect on total n-3 fatty acids except for deep-fried fillets. Cooking methods had no significant effect on total n-6 fatty acids. In all the cooking methods, n-6 fatty acids were well preserved. There was no significant difference in the Mn, Zn, Ca and Fe contents of fillet between raw and cooked samples. Vitamin D content in all cooking methods did not differ significantly. None of cooking methods had a significant effect on vitamin A except for deep-frying fillets. However, vitamin B1 and B3 contents of cooked fish significantly decreased. Considering the overall nutritional quality indices, vitamin and mineral contents, steaming is the best cooking method.     

Effects of cooking and reheating methods on the fatty acid profile of sea bream treated with rosemary extract

BACKGROUND: The effects of rosemary extract on the fatty acid profile of sea bream fillets cooked by different methods (oven baking, grilling and pan frying) as well as the effects of different reheating methods (microwave and conventional oven) on the fatty acid composition of fish after frozen storage for 4 months were investigated. RESULTS: The proportion of saturated fatty acids increased only slightly in fried samples but significantly in oven‐baked and grilled samples, while the proportion of polyunsaturated fatty acids (PUFAs) increased significantly in fried samples but only slightly in oven‐baked and grilled samples. The proportion of monounsaturated fatty acids remained relatively constant after cooking. Of the fatty acids analysed, the most significant increases (P < 0.05) were observed in C18:1n‐9 and C18:2n‐6 and the most significant decreases (P < 0.05) in C14:0, C16:1, C20:5n‐3 and C22:6n‐3. Although sea bream fillets fried in sunflower oil showed an increase in PUFAs, the lowest eicosapentaenoic and docosahexaenoic acid contents were found in fried samples. CONCLUSION: Sea bream fillets treated with rosemary extract showed slower oxidation than untreated fish. Neither conventional nor microwave reheating after frozen storage for 4 months had a detrimental effect on the fatty acid profile and its stability. Copyright © 2009 Society of Chemical Industry     

Seasonal changes in the proximate chemical compositions and fatty acids of chub mackerel (Scomber japonicus) and horse mackerel (Trachurus trachurus) from the north eastern Mediterranean Sea

The effects of seasonal variations on the proximate chemical compositions and fatty acid profiles of chub mackerel (Scomber japonicus) and horse mackerel (Trachurus trachurus) captured in the north‐eastern Mediterranean Sea were investigated. Protein fluctuations were observed in two species for all seasons. The lipid content of both species was lower in winter than in autumn and spring. In all seasons, the major fatty acids in both species were observed to be palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1 ω9), eicosapentaenoic acid (20:5 ω3) and docosahexaenoic acid (20:6 ω3). Chub mackerel and horse mackerel exhibited seasonal fluctuations in their fatty acid contents. The fatty acid profile of the two species had a higher degree of unsaturation during winter. The levels of EPA in chub mackerel in winter, spring and autumn were 5.96%, 4.86% and 4.33%, respectively, while those of DHA were 24.94%, 18.75% and 17.12%, respectively. The levels of EPA in horse mackerel in winter, spring and autumn were 5.42%, 5.03% and 4.86%, respectively, while those of DHA were 14.96%, 13.31% and 11.10%, respectively. The PUFA (polyunsaturated fatty acids) values and ω3/ω6 ratios in the two species were highest in winter. The results indicate that chub mackerel and horse mackerel captured in the north‐eastern Mediterranean Sea, which are among the most important fish in Turkey and of international commercial value, are a good source of nutrition for human consumption in terms of their proximate chemical composition and fatty acids.     

Effect of frozen storage on the quality of whole fish and fillets of horse mackerel (Trachurus trachurus) and mediterranean hake (Merluccius mediterraneus)

 Whole fish and fillets of horse mackerel (Trachurus trachurus) and mediterranean hake (Merluccius mediterraneus) were assessed for quality (physical, chemical and sensory attributes) changes throughout 12 months of frozen storage at −18 °C. The pH, expressible water (EXW), quantities of trimethylamine (TMA), dimethylamine (DMA), formaldehyde (FA), the total volatile base nitrogen (TVB-N) the thiobarbituric acid number (TBA), peroxide value (PV) and amount of free fatty acids (FFA) increased, while sensory attributes (odour, taste, texture) decreased during the frozen storage period. A comparison of quality scores between whole fish and fillets of horse mackerel and mediterranean hake showed that there were no significant differences (P>0.05) in attribute scores. There were, however, significant differences (P<0.05) in pH, EXW, TMA, DMA, FA, TVB-N, TBA, FFA and PV. Received: 19 April 1996/Revised version: 7 September 1996     

Effects of Soy and Corn Flour Addition on Batter Rheology and Quality of Deep Fat-Fried Shrimp Nuggets

In this paper, the effects of soy and corn flour (5 and 10% w/w) addition to the batter formulation on the quality of deep fat-fried shrimp nuggets were evaluated. Rheological properties of batters, coating pick-up, moisture content, and oil content of the samples were determined. Shrimp nuggets were fried at 150 °C, 170 °C, 190 °C, for 1, 2, 3, 4, and 5 min. The coating pick-up, oil and moisture content were found to be directly proportional to batter viscosity. All batters were found to show shear thinning behavior by exhibiting flow behavior index ≤ 1. The batters were modeled as power law fluids. Batter formulation, frying time, and temperature significantly (p < 0.01) affected moisture and oil content of shrimp nuggets. Soy flour added batters provided the highest consistency index, 7.595 and 10.635 Pa.sⁿ for 5% and 10% soy flour added, respectively. Soy flour was found to be an effective ingredient in decreasing oil content of fried nuggets. Batters containing 5% corn flour showed the lowest moisture content and the highest oil content among all the formulations. The mean moisture and fat content of shrimp nuggets coated with batter contained 10% soy and 5% corn flour, fried at 190 °C, for 5 min were 0.59 ± 0.022, 0.480 ± 0.029 and 0.149 ± 0.035, 0.346 ± 0.024 (g/g db), respectively.     

Modeling and Optimization of Enzymatic Degradation of Aflatoxin B1 (AFB1) in Red Chili Powder Using Response Surface Methodology

Response surface methodology was used to optimize the peroxidase-catalyzed enzymatic degradation of aflatoxin B₁ (AFB₁) from red chili powder. Twenty experiments were carried out using central composite rotatable design with three independent variables (enzyme concentration, substrate or AFB₁ concentration, and incubation time) and single response (% aflatoxin B₁ degradation). The optimum conditions achieved after numerical and graphical optimizations for maximum percent degradation were: AFB₁ (31.5 nM), enzyme (13.5 U/nM AFB₁), and time (26 h). The actual percent degradation achieved at these optimum conditions was 70.0% and predicted 74.5%. The reduced quadratic model developed for the experimental data was found adequate to describe the relationships between the operating variables (Model F = 21.61, p < 0.001; F _lof = 3.63, p > 0.05). The robustness of the model was tested by confirmation experiments, and difference was found insignificant between the actual and predicted values as confirmed by two-tailed t test (α = 0.05). The hepatotoxic effect of the AFB₁ pre- and post-detoxification was tested on Wistar rats. The detoxified powder was also tested for changes in ascorbic acid, β-carotene, ASTA color value, and capsaicin content. Capsaicin was resistant to enzymatic degradation under specified conditions, but loss of around 15% was reported in color and β-carotene content; the loss in ascorbic acid was 10%.     

Use of focused open vessel microwave-assisted extraction as prelude for the determination of the fatty acid profile of fish – a comparison with results obtained after liquid-liquid extraction according to Bligh and Dyer

 A method based on microwave-assisted extraction (MAE) was developed to extract lipids from fish for the determination of the fatty acid composition. Microwave-assisted extraction was performed with an open-vessel extraction apparatus similar to the system of Soxhlet. The solvent was an equivolume mixture of ethyl acetate and cyclohexane. The solvent forms a ternary azeotrope with water, and water is separated after re-condensation in a water trap. With this technique, extraction can be performed without pre-drying of fish tissues. After lipid extraction, the esterification was performed with trimethylsulfonium hydroxide (TMSH) and the fatty acid methyl esters (FAMEs) were determined by GC/FID. The results were compared with those obtained after liquid-liquid extraction according to Bligh and Dyer. Fillets of mackerel (Scomber scombrus) and livers of cod (Gadus morhua) were used as the sample material. The water content (n=7) of the cod livers was 36.2±1.6% and that of mackerel fillets was 74.5±0.5%. The lipid contents (n=5) using the MAE method were 5.6±0.4% and 62.6±3.1% in mackerel and cod liver, respectively. Relative levels of 58 fatty acids (two co-eluted) were determined with GC/FID on two capillary columns with different polarity. These were 10 saturated, 24 unsaturated, and 24 unidentified peaks which are most likely unsaturated or branched-chain fatty acids. After MAE the composition of the 57 peaks was virtually the same as that obtained with the Bligh and Dyer method. The advantages are, however, that MAE is faster, requires less solvent, and avoids the use of chlorinated solvents and so is more environmentally friendly. Our results confirm that MAE is a well-suited alternative to the Bligh and Dyer method for the extraction of lipids followed by determination of the fatty acid profile. Received: 1 March 2000 / Revised version: 23 May 2000     

Sterol Content of Fish,Crustacea and Mollusc: Effects of Cooking Methods

Sterol contents (desmosterol, cholesterol, stigmasterol, and sitosterol) of 36 fresh and cooked seafood and four freshwater fish species were investigated by high performance liquid chromatography (HPLC) method. Cholesterol and sitosterol were main sterols in seafood and freshwater fish species. Raw shellfish and molluscs contained cholesterol above 18.92 mg/100 g fish muscle, while the cholesterol content of marine fish ranged from 6.5 to 78.40 mg/100 g fish muscle. The cooking process resulted in significant effects on the sterol contents of seafood and freshwater species (p < 0.05). A remarkable increase in sitosterol (more than 3–4 fold compared to raw fish) was recorded for some fish species cooked in the oven. The highest desmosterol content was observed for fried fish, whereas the frying process resulted in a significant loss in cholesterol and sitosterol contents of marine fish (p < 0.05). The impacts of cooking methods on sterol content of seafood and their form varied depending on fish species and the cooking method used.     

The nutrient composition of three cuts obtained from P-class South African pork carcasses

The shoulder, loin and leg from P-class pork carcasses were used to determine the nutrient composition of both raw and cooked cuts. Significantly lower fat content were observed in the current study for the leg (5.21 g/100 g) and loin (6.99 g/100 g) compared to the shoulder cut (10.32 g/100 g). The overall percentage fat for all three cuts was less than 10% which is recommended by the South African Heart Mark. The cooked loin cut contained the most protein (27.50 g/100 g) of the three cooked cuts. When compared to other meat products (beef, mutton and chicken) it is clear that pork is a good source of B vitamins, especially vitamin B₃. The cooked loin cut contained the least vitamin B₁ (0.22 mg/100 g), B₂ (0.02 mg/100 g) but the most vitamin B₃ (7.09 mg/100 g), of the three cooked cuts. The 100 g cooked shoulder, loin and leg cuts provide on average 40.11% protein, 5.19% magnesium, 3.37% calcium, 24.29% phosphorus, 18.22% zinc, 22.33% iron and 22.50% vitamin B₁, 2.57% vitamin B₂ and 42.6% vitamin B₃ of Recommended Daily Allowances for males, age 25–50. Energy from a 100 g portion provides 5.81% of the Recommended Daily Allowances. To conclude, the pork cuts are undoubtedly a good source of nutrients that is required for good health because it is high in protein, have a low fat content and are a nutrient-packed choice for the family and compares favourably with the fat, energy, and cholesterol content of many other meats and poultry.     

Capability of the rosemary (Rosmarinus officinalis) extract on the oxidative stability of cooked sea bream (Sparus aurata) during frozen storage

The effects of rosemary extract addition on oxidative stability of cooked sea bream during frozen storage period were investigated. Sea bream fillets were fried, oven baked and grilled. Three groups with rosemary extract were also cooked by the same methods and cooked samples (with and without rosemary extract) were stored in −18 °C for 4 months. After cooking procedures, the FFA formation was quite similar in fresh-raw and fried sea bream fillets, but significantly increased oven baked (0.31–0.33% oleic acid) and grilled fillets (0.39–0.40% oleic acid). The highest value of PV was also obtained from grilled fillets (1.18–1.58 meq/kg). However, TBA values (1.92 mg MA/kg for fresh-raw fillet) did not increase in sea bream fillets after the frying and grilling procedure except oven baking without rosemary extract (2.41 mg MA/kg). A considerable increase in the FFA, PV and TBA was observed in all cooked groups during frozen storage period. The treated samples with rosemary extract generally showed slower PV and TBA formation than those of the untreated samples. However, the additions of rosemary extract have positive effect on sensory quality of baked sea bream.