共查询到19条相似文献,搜索用时 109 毫秒
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以木犀草素为模板分子,偶氮二异丁腈为引发剂,丙烯酰胺为功能单体,N,N-亚甲基双丙烯酰胺为交联剂,在极性溶剂中合成了木犀草素分子印迹聚合物(LMIP)。考察了溶剂用量及种类和功能单体对合成的影响,得到了最佳合成工艺条件,简化了模板分子的洗脱工艺。红外光谱分析表明:洗脱前分子印迹聚合物中原木犀草素在1 270 cm-1处的O—H吸收峰移至1 300 cm-1处,说明模板分子被功能单体、交联剂经聚合交联固定在聚合物中,洗脱后该峰完全消失;扫描电子显微镜观察发现:洗脱后的分子印迹聚合物表面凹凸不平,产生了大量吸附模板分子的空穴。探讨了LMIP的吸附特性,用与木犀草素结构相似的槲皮素作对比实验,分子印迹聚合物对木犀草素的吸附量(33.653μmol/g)明显高于槲皮素的吸附量(8.654μmol/g),表明LMIP对模板分子具有较高的吸附选择性。 相似文献
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以对羟基苯甲酸乙酯为模板,α-甲基丙烯酸为功能单体,甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,用沉淀聚合法制备对羟基苯甲酸乙酯分子印迹聚合微球.通过一系列的单因素实验优化了聚合反应条件,通过正交实验得出,在模板分子、功能单体、交联剂物质的量比例为1∶6∶20,反应温度为65℃的条件下合成的印迹聚合物的吸附量最大.以紫外,可见分光光度计对印迹聚合物的吸附性、选择性进行了分析.以优化后的反应条件合成的印迹聚合物对对羟基苯甲酸乙酯的相对吸附量为866.55 μg/g,同等条件下非印迹聚合物的结果为406.72 μg/g. 相似文献
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以黄曲霉毒素结构类似物7-乙酰氧基-4-甲基香豆素为替代模板,甲基丙烯酸缩水甘油酯为单体,氯化苄为引发剂,氯化亚铜为过渡金属卤化物,2,2-联吡啶为配体,利用原子转移自由基聚合法合成分子印迹聚合物,通过一系列实验对其合成条件进行优化;对聚合物进行表征,并对其吸附黄曲霉毒素的能力进行研究。通过单因素实验和正交试验,得到最佳的合成条件:模板与功能单体的物质的量比为1∶80,引发剂的量为1.5%(单体的质量百分比),反应温度为60℃,过渡金属卤化物与配体的物质的量比为1∶3。在最优的合成条件下合成分子印迹聚合物,并将其作为填充材料制作固相萃取柱,用于选择性分离样品中的黄曲霉毒素,并与商品柱对比。 相似文献
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研究了Cr6+印迹聚合物的制备方法,以C(rVI)为模板分子,4-乙烯基吡啶和甲基丙烯酸羟乙酯为功能单体,二甲基丙烯酸乙二醇酯为交联剂,在偶氮二异丁腈引发下,采用溶液聚合法制备了3种C(rVI)离子印迹聚合物。研究了硫脲脱除模板离子的条件,并对印迹聚合物的吸附效果进行了初步评价。实验结果表明,3种印迹聚合物对C(rVI)具有很好的吸附效果,有望成为Cr6+污染治理的高效吸附剂。 相似文献
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以牛血红蛋白为模板分子,丙烯酰胺为功能单体,N - N甲基双丙烯酰胺为交联剂,通过固定在硅胶表面的引发转移终止剂二乙基二硫代氨基甲酸钠引发聚合反应,应用表面印迹法制备了牛血红蛋白(BHb)印迹聚合物.通过平衡吸附和选择性实验进行评价,结果表明印迹聚合物具有高吸附效率和选择性. 相似文献
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采用分子印迹技术,以2,2',4,4'-四羟基二苯甲酮为模板分子,2-乙烯基吡啶为功能单体,二甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁氰为引发剂,在乙腈溶液中合成2,2',4,4'-四羟基二苯甲酮分子印迹聚合物。通过平衡吸附和液相色谱的方法分析了印迹聚合物的识别特性。研究结果表明,聚合物对印迹分子具有很好的亲和性和特定的选择性,用作液谱固定相可将其与结构类似物2,4-二羟基二苯甲酮、2-羟基-4-甲氧基二苯甲酮快速基线分离。 相似文献
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Microsphere silica (AMS) was prepared by the sol–gel method and used for the synthesis of dye‐molecular‐imprinted polysiloxanes. These were prepared by the cohydrolysis and polycondensation of the AMS with functional organosilicone monomers (N‐phenylaminomethyl triethoxysilane, 3‐aminopropyl triethoxysilane, and 4‐aza‐6‐aminohexyl triethoxysilane) in the presence of a template molecule of disperse red (or disperse blue or water‐soluble dye) followed by the removal of the template molecule. The molecular‐imprinting and nonimprinting polysiloxanes were characterized by elemental analysis, diffuse‐reflectance Fourier transform infrared spectroscopy, and scanning electron microscopy. Their adsorption behavior toward the template molecule and nontemplate molecule was investigated. The molecular‐imprinting polysiloxane exhibited higher selective recognition properties toward the template molecule than the nonimprinting polysiloxane. In the experimental conditions, their adsorption isotherms were a good fit with the Langmuir model. The distribution coefficient value of water‐soluble‐dye‐imprinted polysiloxane was the highest among the three imprinting polysiloxanes under the experimental conditions. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 637–643, 2004 相似文献
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Elijan Amut Qiang Fu Qi Fang Rong Liu Aiping Xiao Aiguo Zeng Chun Chang 《Journal of Polymer Research》2010,17(3):401-409
Amlodipine is a first-line therapeutic drug for hypertension and angina pectoris and its vasodilating effect only resides
in the S-(-)-enantiomer. But mostly commercial chiral separating matrixes are not capable of enantio-separating of racemic amlodipine.
The amlodipine’s the chiral molecular imprinting polymers monolithic column (CMIPMC) was firstly and successfully synthesized
by in situ molecular imprinting technique, utilizing S-(-)-amlodipine as the template molecule, methacrylic acid (MAA) as a monomer, and the resulting monolithic colunm could be
directly integrated into the high performance liquid chromatography (HPLC) systems. The scanning electron microscope (SEM)
was employed to identify the micro-morphologic features of the obtained polymers. Further, the molecule-special recognition
mechanism and special selective absorbent properties for the corresponding template were studied by chromatographic system
and Scatchard analysis model, respectively. Meanwhile, the optimized chromatographic conditions for chiral separation of amlodipine
enantimoers were established. The results showed the obtained CMIPMC had a high specific affinity and selectivity for the
template molecule S-(-)-amlodipine, in which chiral separation of racemic amlodipine was achieved under optimized conditions. The mechanism investigation
results showed that two kinds of binding sites prevail in the CMIPMC, besides the molecular shape complementation of template
molecular and CMIPMC, hydrogen bond or ionic interaction seem to play an important role in the enantio-selective recognition
of the CMIPMC. The research laid fundament for the study of expanding the amlodipine chiral separating medium. 相似文献
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计量置换模型在分子印迹聚合物色谱分离中的应用 总被引:3,自引:1,他引:3
以N–叔丁氧羰酰–L–色氨酸为印迹分子,制备了分子印迹聚合物. 色谱评价的结果表明该聚合物对印迹分子有特异选择性的吸附作用. 依据液相色谱溶质计量置换保留模型(SDM–R),分析了印迹分子与固定相和流动相分子间的相互作用机理. 发现在以异丙醇、乙腈混合液为流动相时,印迹分子与固定相吸附时覆盖的活性位点数量是其对映体的1.2倍;印迹分子失去的溶剂分子数是其对映体的1.5倍. 可以认为, 在这种条件下,分子识别作用主要是由于分子印迹聚合物中的三维孔穴结构. 相似文献
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在壳聚糖表面通过希夫碱反应嫁接一层戊二醛,形成核-壳结构微球。然后在这个核-壳微球上,以胰蛋白酶为模板分子,3-氨基苯硼酸为功能单体,制备了胰蛋白酶分子印迹聚合物,通过静态吸附法,研究了聚合物的吸附性能。结果表明,印迹聚合物对模板分子有较高吸附容量和特异选择性。为从蛋白质混和溶液中分离富集胰蛋白酶提供了新的材料和方法。 相似文献
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分子印迹聚合物制备与应用 总被引:11,自引:0,他引:11
分子印迹作为制备对某一特定的分子 (印迹分子或模板分子 )具有特异性识别的聚合物的过程 ,在分离分析、仿生传感器和模拟酶催化等方面具有重要的应用前景。介绍了分子印迹技术的基本原理、分子印迹聚合物的制备和特性、分子印迹技术的应用场合及发展趋势 相似文献
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The aim of this study is to prepare flavone imprinted silica with good sphericity. Previous studies have demonstrated that imprinted silica can be synthesized using a simple sol‐gel method and a grinding‐sieving process. Despite the simplicity of the synthetic method, the appearance of the final product obtained is often very irregular and nonuniform. This product has many utilization issues and problems, e.g., residues and low operability. In this study, one type of novel spherical flavone imprinted silica was synthesized. A sol‐gel method and molecular imprinting technique were employed. By using luteolin as a template molecule, aminopropyltriethoxysilane (APTS) as the functional monomer, tetraethoxysilane (TEOS) as the cross‐linking agent, molecularly imprinted silica microspheres were prepared via a two‐stage reaction. The preparation conditions were monitored by the appearance of the products. SEM images show that under optimum conditions, the diameters of these microspheres are ca. 20–30 μm. The amino group adsorption peaks can be seen from the IR spectra. Results from elemental analysis indicate that 3.206 μmol of amino groups were successfully introduced into 1 g of product. This microspheric adsorbent has a high adsorption capacity to the template molecule, high adsorption selectivity and reproducibility. The adsorption capacity amounted to 32.987 mg/g and the separation factor was up to 84.3, while the adsorption capacity hardly decreased after 15 cycles. 相似文献