Effect of apple cultivar and enzyme treatment on phenolic compounds content during clear apple juice production

The aim of the investigation was evaluation of phenolic compounds content, measured by HPLC method at selected stages of clear apple juice production with pectolytic mash enzymation from ‘Shampion’ and ‘Idared’ apples. Sum of phenolic compounds in ‘Shampion’ mash was significantly higher than in ‘Idared’ mash, 520 and 352 mg kg^?1, respectively. Enzymation of ‘Idared’ mash resulted in a higher phenolic compounds decrease compared to ‘Shampion’, 23% and 14% respectively. Sum of phenolics in juices from ‘Shampion’ for Panzym at 50 °C (366 mg kg^?1) was significantly higher than in juices from ‘Shampion’ for Rohapect at 20 °C and ‘Idared’ for Panzym at 50 °C (256 and 234 mg kg^?1, respectively). The lowest phenolics (95 mg kg^?1) was in juices from ‘Idared’ for Rohapect at 20 °C. There was a positive correlation (R² = 0.933) between phenolics content and antioxidant activity of juices (ABTS^?+). In general, enzymation at 50 °C with Panzym lead to a higher content of the phenolic compounds in juices compared to Rohapect at 20 °C.     

Effect of 1-MCP on quality and antioxidant capacity of in vitro digests from ‘Sunrise’ apples stored at different temperatures

The antioxidant capacities of phenolic and non-phenolic fractions for in vitro digestates from ‘Sunrise’ apple were assessed after postharvest application of 1-methylcyclopropene (1-MCP), a ripening inhibitor, and three weeks storage at 5, 13, 15, 18 and 22 °C. An in vitro digestion system was used to generate the soluble bioaccessable digestate which was then fractionated into phenolic and non-phenolic fractions. The two fractions were assayed for Folin-Ciocalteu Reaction (FCR) reducing capacity and peroxyl radical scavenging capacity. Quality retention of the fruit was assessed by measuring internal ethylene concentration, firmness and titratable acidity. Treatment with 1-MCP inhibited internal ethylene concentration and better maintained the firmness and titratable acidity of ‘Sunrise’ summer apples as compared with untreated control apples at storage temperatures of 15 °C and above. The FCR reducing capacity of the phenolic fraction of the in vitro, simulated gastrointestinal digestates showed similar response as did the quality measures, with significantly higher activity in the 1-MCP treated fruit at higher storage temperatures. However, no consistent differences were found between 1-MCP and control treatments for the FCR reducing capacity of the non-phenolic fraction or for the peroxyl radical scavenging capacity of either fraction. The non-phenolic fractions consistently had higher levels of both types of antioxidant capacities. Treatment and storage of ‘Sunrise’ apples at elevated temperatures (> 13 °C) resulted in improved fruit quality and retention of reducing capacity in simulated gastrointestinal digestates.     

Effect of l-ascorbic acid addition on quality, polyphenolic compounds and antioxidant capacity of cloudy apple juices

Polyphenols are one of the most important dietary antioxidants, and the apples, being a major source of polyphenolic compounds. There is increasing evidence that the consumption of fruit and their preserves, which are rich sources of vitamins and phenolics, is negatively associated with lung cancer and coronary heart disease. The influence of ascorbic acid addition on the quality, polyphenolic profile and antioxidant capacity in 14 selected varieties of apple juices during the 3 years of study was examined. Evaluation of changes of ascorbic acid content allowed to demonstrate the differences in susceptibility of apples on the enzymatic oxidation. The study shows that the variations of the least susceptible to oxidation of ascorbic acid are ‘Shampion’ and ‘Topaz’, and to a lesser extent, ‘Szara Reneta’, ‘Arlet’ and ‘Jonafree’ apples. A total of 17 kinds of polyphenolic compounds including catechins, procyanidins, hydroxycinnamates, flavonols, anthocyanins and dihydrochalcones were identified in apple tissues by HPLC with DAD analysis and after phloroglucinolysis and LC–MS. During the 3 years of study, the addition of ascorbic acid positively affects on the polyphenol compounds and antioxidant capacity (DPPH, ABTS and FRAP), increasing their content in the analyzed samples by 65 and 433, 328 and 300 %, respectively. The correlation coefficient between DPPH, ABTS and FRAP antioxidant capacity and ascorbic acid was 0.71, 0.71 and 0.72, respectively.     

1-MCP对‘粉红女士’苹果果实采后生理及其品质的影响

为探讨1-甲基环丙烯(1-methylcyclopropene,1-MCP)处理对‘粉红女士’苹果采后生理特性及其品质的影响。用1.0 μL/L的1-MCP处理‘粉红女士’苹果24 h,以不处理为对照,20℃条件下贮藏,定期测定贮藏期间的主要品质及相关生理指标。结果表明:1-MCP处理可保持苹果贮藏过程中的硬度、可滴定酸含量,延缓果皮油腻化的发生,显著抑制果实的呼吸强度和乙烯释放速率,推迟果实过氧化氢酶、过氧化物酶两种酶活性峰值的出现,并在一定程度上提高了超氧化物歧化酶和过氧化氢酶的活性,有效抑制丙二醛含量的积累。因此,1-MCP处理可较好地调控‘粉红女士’苹果的相关生理代谢,改善果实的贮藏品质,从而有效延缓果实采后的成熟衰老进程。     

Effect of heat treatment on physiochemical,colour, antioxidant and microstructural characteristics of apples during storage

Changes in qualities of ‘Red Fuji’ and ‘Golden Delicious’ apples in response to different heat treatments (45 and 60 °C) following cold storage were investigated. Quality indices including firmness, titratable acidity, total phenolic content, antioxidant capacity, colour parameters and microstructure were measured. Results indicated heat treatment at both temperatures for 3‐h hastened loss of titratable acidity in ‘Red Fuji’ and ‘Golden Delicious’ apples, but maintained firmness in both cultivars. However, heat treatment at lower temperature (45 °C) best maintained total phenolic compounds and antioxidant capacity in ‘Red Fuji’ apples throughout storage, whereas there was no difference between heat treatments in ‘Golden Delicious’ apples. Examination by scanning electron microscope (SEM) revealed that the effect of heat treatment at different temperatures on ‘Red Fuji’ and ‘Golden Delicious’ apples was structurally different. Apple tissue that had been heat treated at elevated temperature (60 °C) for 3 h was associated with fractured cell walls and collapse of cells.     

Polyphenol content and antioxidative activity in apple purées with rhubarb juice supplement

The purpose of the present study was to evaluate the effect of rhubarb juice addition and peeling in apple purée production and storage on its phenolic composition, antioxidant activity and colour. The apple material used in this study was of two varieties: Sampion and Idared. The apple purées prepared in three variants (control purées, peeling purées and purées with 5% of rhubarb juice) were stored for 3 and 6 months at 30 °C. Apple purées were investigated for their antioxidant activity, change of colour and contents of phenolic compounds plus the polymer procyanidins and degree of polymerization (DP). The preparation of purées from apples without peeling and the addition of rhubarb juice during purée preparation had significant influence on polyphenol content. The purée prepared from peeled apples of Idared variety had phenolic content 2.2 times lower than the control sample (non‐peeled apples). The highest level of total polyphenols was found in Idared + rhubarb sample (129.82 mg 100 g^?1 purée). The content of this compound in Idared control sample was more than 2.8 times lower than in Idared purées with rhubarb. The use of non‐peeled Idared apples and 5% of rhubarb juice in purée preparation significantly increased polymeric procyanidins from 11.68 to 75.20 mg 100 g^?1 and other phenolics from 9.23 to 54.62 mg/100 g. This effect for Sampion apples was smaller. The procyanidins during purées storage were more stable in samples with rhubarb juice addition. The purée samples with the addition of rhubarb juice had higher antioxidant activity measured using ABTS (2,2′azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonic acid)) than purée samples without the addition (two times higher for Idared variety) of rhubarb juice. After 3 and 6 months of storage at temperature 30 °C, the antioxidant capacity decreased in all purées. Rhubarb juice had only high and positive effect on L* value in the case of Idared variety. The results showed that all Sampion apple purées had higher L* values than Idared.     

采后1-MCP和MAP处理对‘红富士’苹果冷藏和货架期品质的影响

对‘红富士’苹果进行1.0 μL/L 1-甲基环丙烯（1-methylcyclopropene,1-MCP）和自发气调包装（modified atmosphere package,MAP）处理,继而进行0 ℃冷藏和20 ℃货架贮藏。结果表明：贮藏期间,‘红富士’苹果果实硬度和可滴定酸含量下降,可溶性固形物含量升高,虎皮病和果心褐变增多。1-MCP处理能较好维持冷藏期间‘红富士’苹果果实硬度和可溶性固形物含量,降低了包装内CO2和乙烯含量。同时,1-MCP明显降低了冷藏期间虎皮病发病指数、果心褐变指数以及果柄端果肉褐变率,显著抑制果皮α-法尼烯及共轭三烯的生成。1-MCP＋MAP结合使用可较好维持果实可滴定酸含量和果皮色泽、抑制果柄端果肉褐变。综合分析认为,1-MCP＋MAP处理能较好维持‘红富士’果实冷藏和货架期间的品质,并显著抑制果实虎皮病的发生。     

MODELING THE SENSORY QUALITIES OF PROCESSED APPLES AFTER CONTROLLED ATMOSPHERE STORAGE AND FROZEN STORAGE1

Durational effects of controlled atmosphere storage (CA) and frozen storage on apple quality were studied for commercially processed pies made from Northern Spy, Idared and Nova Spy apples. Sensory tests on apple pie quality indicated that the duration of apples in CA significantly affected the quality of the processed apple. Extending the CA duration of the apples to 45 weeks resulted in a pie filling with reduced apple flavor, tartness and astringency, and with increased sweetness and off‐flavor. To minimize flavor/taste changes throughout the processing season, close attention must be given to the product's sugar/acid formulation: by week 27 for Idared apples and by week 33 for Northern Spy and Novaspy apples. The texture and appearance of processed Novaspy and Idared fruits were affected earlier in CA than Northern Spy apples. The frozen product was relatively stable for all three varieties, especially for apples processed early in the storage season (15–27 weeks). A strategy that utilizes short‐ to medium‐term CA with longer‐term frozen storage would optimize quality across the season.     

1-MCP、MAP对苹果冷藏期间品质及保鲜效果的影响

以华红苹果为材料,研究1-MCP和小包装自发气调包装(MAP)对苹果冷藏(0℃)期间品质的影响。结果表明：1-MCP和MAP均可有效控制华红苹果果实货架期返糖现象的发生,抑制果实货架期间呼吸强度和乙烯释放量的增加,减缓果实硬度、可溶性固形物含量和可滴定酸含量的下降,抑制果皮亮度和细胞膜透性的增加。其中,0.02mm PE袋包装和0.04mm PVC袋包装均未使华红苹果产生CO2伤害,为较耐CO2品种。果皮亮度和细胞膜透性可作为华红苹果衰老与否的衡量指标,可溶性固形物和可滴定酸含量可作为华红苹果品质维持好坏的衡量指标。包装方式延长贮藏时间的顺序为：1-MCP＞0.04mm PVC袋＞0.02mm PE袋＞对照;包装方式维持果实品质能力的顺序为：0.04mm PVC袋＞1-MCP＞0.02mm PE袋＞对照。     

Effect of 1-Methylcyclopropene on the Quality of Fresh-cut Apple Slices

ABSTRACT: The objective of this project was to treat fresh-cut apple slices with the ethylene inhibitor 1-methylcyclopropene (1-MCP) to retard ethylene-induced deterioration and senescence and to prolong the shelf life of such products. Intact Braeburn and Pacific Rose apples were treated at different times with 1-MCP, cut, and stored at 0 °C. Samples were analyzed initially and then weekly for ethylene, respiration, flesh firmness, tissue color, titratable acidity, and soluble solids content during 5 wk of storage. 1-MCP treatment was effective in reducing ethylene production, respiration, and loss of firmness and color of slices when applied to whole apples directly after harvest. Total sugar and acidity levels were not affected significantly.     

中早熟苹果品种的贮藏品质和酚类物质差异分析

中早熟苹果品种可以丰富水果市场,是苹果产业重要的组成部分,研究其贮藏特性及品质差异意义重大。本实验以‘鲁丽’‘鲁艳’两个苹果新品种为研究对象,以其亲本‘嘎啦’为参照,探究3种苹果的贮藏品质和酚类物质差异。结果表明：相比于‘嘎啦’苹果,‘鲁丽’苹果可溶性固形物质量分数最高,果皮a*值最大,外观品质最佳、适于鲜食,而‘鲁艳’苹果总酸度高、固酸比低,贮藏期间乙烯释放量高,不耐贮藏。与‘嘎啦’和‘鲁艳’苹果相比,‘鲁丽’苹果有着最高的总酚、总黄酮含量和1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基清除率,抗氧化能力最强。3种苹果主要的酚类物质也因品种、成熟度和果实部位存在差异,‘嘎啦’和‘鲁丽’苹果果肉均以绿原酸含量最高,而‘鲁艳’苹果表儿茶素含量最高,果皮酚类物质含量高于果肉。相关性和主成分分析表明果实硬度与a*值有高度显著负相关性（P<0.001）,可溶性固形物质量分数与总酚、总黄酮、绿原酸含量和DPPH自由基清除率存在高度显著正相关（P<0.001）,主成分1主要由总酚、总黄酮、绿原酸、表儿茶素含量等指标决定,成熟...     

Influence of apple purée preparation and storage on polyphenol contents and antioxidant activity

The polyphenolic components of Idared and Shampion apple purées were determined by HPLC; chlorogenic acid was the most abundant acid (20.0 mg/100 g in microwaved Idared); other polyphenols identified in high concentrations included (−)-epicatechin, procyanidin B1 and B2; quercetin and cyanidin glycosides were found in minor concentrations. The Shampion purées had higher total phenolics (142 mg/100 g) and procyanidin B2 concentrations (17.3 mg/100 g) than the Idared ones, and polymeric procyanidins represented 41% of all polyphenols. Antioxidant capacities of the samples, determined by spectrophotometric methods and EPR spectroscopy, correlated well with the concentration of polyphenols. The antioxidant properties could be better represented by EPR than by UV–vis measurements. The latter require transparent (clear) samples whereas EPR can be a method of choice in the assessment of radical-scavenging activity of dense and cloudy apple purées. Our results support the putative high antioxidant value of apple purées and define their capacity in terms of the major constituents. Apple purées are a rich source of natural antioxidants, especially of chlorogenic acid and procyanidins.     

1-MCP处理对苹果采后常温贮藏品质的影响

以‘红富士’和‘黄元帅’两个品种苹果为实验材料,研究1-甲基环丙烯（1-methylcyclopropene ,1-MCP）处理后苹果在20 ℃常温贮藏条件下果实品质的变化,探讨1-MCP对短期贮藏苹果品质的影响。结果表明,与对照相比,1 μL/L 1-MCP处理对‘红富士’和‘黄元帅’苹果的质量损失率、硬度及可溶性固形物含量无显著影响（P＞0.05）;处理组可滴定酸含量在7 d后显著高于对照组（P＜0.05）;‘红富士’苹果1-MCP处理在14 d后可以延缓pH值上升,而‘黄元帅’处理组7 d后即可延缓pH值升高;1-MCP处理对‘红富士’VC含量无显著影响（P＞0.05）,而‘黄元帅’中1-MCP组VC含量在第1天开始即显著高于对照组（P＜0.05）。由此可见,1-MCP处理对不同品种苹果不同品质指标的影响不同。     

不同品种苹果冷藏期间品质与生理变化

研究贮藏期间果实品质生理变化,探讨不同优质苹果品种的贮藏特性。以‘岳帅’、‘望山红’、‘岳阳红’、‘秋富红’苹果为试验试材,在温度（0±0.5）℃、相对湿度90%～95%冷库内贮藏,定期测定果实呼吸强度、硬度、可溶性固形物含量、可滴定酸含量、VC含量、果实质量损失率、多酚氧化酶活性、过氧化物酶活性,分析果实生理品质指标变化与贮藏特性的关系。结果表明,‘岳帅’苹果呼吸强度及酶活性较低,但呼吸高峰出现早,贮藏84 d后果实品质劣变迅速;‘岳阳红’苹果呼吸强度和酶活性较低,贮藏期间果实品质、风味保持最好;‘望山红’和‘秋富红’苹果呼吸高峰出现最晚,果实品质保持较好。通过理化指标和感官评价比较得出：‘岳帅’贮藏期较短,不宜超过84 d;‘岳阳红’、‘望山红’和‘秋富红’苹果适宜长期贮藏,其中‘岳阳红’苹果在贮藏过程中品质及营养成分损失最少,贮藏期可达180 d以上。     

The effect of postharvest 1-MCP treatment and storage atmosphere on ‘Cripps Pink’ apple phenolics and antioxidant activity

This study investigated the effect of a postharvest 1-methylcyclopropene (1-MCP) treatment, controlled atmosphere and storage time on the total antioxidant activity (TAA) and phenolic compounds in the peel and flesh of ‘Cripps Pink’ apples (Malus domestica Borkh.). Preclimacteric apples were harvested and treated with 1-MCP then stored in normal atmosphere (NA) or controlled atmosphere (CA) at 0 °C for up to 160 days. In general, the level of phenolics decreased by 9% in the peel and significantly increased twofold in the flesh during cold storage, regardless of storage atmosphere or 1-MCP treatment. However, treatment with 1-MCP resulted in significantly lower concentrations of chlorogenic acid and procyanidin B2 in apple flesh, and catechin and epicatechin in the peel compared to the control fruits. There was no significant effect of CA on the phenolic compounds during long-term storage, except for quercetin 3-galactoside and quercetin 3-glucoside, which both significantly increased under CA storage. Total antioxidant activity (TAA) is an important nutritional attribute of apples in the human diet. The results showed that TAA in the peel tissue was about eight times higher than that of the flesh, with mean values of 4.75 g TE/kg FW and 0.56 TE/kg FW, respectively. The TAA in both the peel and flesh tissue increased significantly during storage by 40% and 70%, respectively. The storage atmosphere did not significantly affect TAA in either the peel or flesh, whilst the 1-MCP treatment significantly reduced the TAA in the peel tissue only. These results show the beneficial combined effects of pre-storage 1-MCP treatment and CA on ‘Cripps Pink’ apple phenolic composition and antioxidant capacity during long term storage.     

Effect of 1-Methylcyclopropene on Superficial Scald Associated with Ethylene Production, α-Farnesene Catabolism,and Antioxidant System of Over-Mature ‘d’Anjou’ Pears After Long-Term Storage

To optimize storage quality of ‘d’Anjou’ pears (Pyrus communis L.) produced in the US Pacific Northwest regions, the recommended harvest maturity as indicated by flesh pressure is 67 to 58 N. Over-mature pears are more susceptible to superficial scald and inferior eating quality after storage. However, a significant portion of pears is harvested at an over-mature stage due to rapid on-tree maturation during years with labor shortages. 1-Methylcyclopropene (1-MCP) extends storage quality of pears but interferes their ultimate ripening capacity. The purpose of this work was to evaluate the effect of 1-MCP on over-mature ‘d’Anjou’ pears. Fruit from two orchards at fruit firmness (FF) 55.6 and 56.0 N were treated with 0.15 μL L^?1 1-MCP, held at ??1.1 °C for up to 7 months. 1-MCP treatment significantly inhibited ethylene synthesis and respiration rate, reduced superficial scald, and maintained high storage quality of over-mature pears from two orchards during 7 months of storage. After 7 months of storage at ??1.1 °C, some of the pears from Orchard 1 recovered ripening capacity after 5 days at 20 °C. The development of superficial scald in over-mature ‘Anjou’ pears was associated with the accumulation of α-farnesene and conjugated trienols (CTols). 1-MCP significantly inhibited the development of scald, perhaps by alleviating membrane lipid peroxidation and enhancing total antioxidant capacity, antioxidant metabolites (i.e., total polyphenols (TP) and total flavonoids (TF)), and related enzyme activities (i.e., superoxide dismutase (SOD), catalase (CAT)) in pear skin. Overall, this study provides the preliminary basis for commercial application of 1-MCP in over-mature pears and demonstrates its potential to maintain fruit quality and reduce the incidence of superficial scald.     

The Effect of 1-Methylcyclopropene (1-MCP) on Quality and Cell Wall Hydrolases Activities of Fresh-Cut Muskmelon (Cucumis melo var reticulatus L.) During Storage

The effects of 1-methylcyclopropene (1-MCP) on quality and cell wall hydrolases activities changes in fresh-cut muskmelon fruit stored at 4?±?1 °C for 7 days were investigated. The results showed that ethylene production of the fresh-cut fruit remained constant during refrigerated storage. 1-MCP treatments delayed the loss of fresh weight and the increase in electrolyte leakage and maintained the firmness of the fresh-cut fruit. The best results are showed in the fresh-cut fruit treated with 1.0 μL L^?1 1-MCP. The use of 1-MCP had no influence on the total soluble solids (TSS) content and pH of the fresh-cut fruit which they remained constant over storage. The titratable acidity (TA) of the fresh-cut fruit decreased during storage and the sample treated with 1.0 μL L^?1 1-MCP showed the lowest data. TSS/TA ratio increased during storage and the TSS/TA ratio was lower in 1-MCP-treated samples than that for the control one. The use of 1-MCP inhibited polygalacturonase (PG), β-galactosidase (β-Gal) and β-galactanase activities, whereas the increases in these enzymes activities were found in the control. Antioxidant capacity and total phenolics content of both 1-MCP-treated and untreated samples remained constant over storage. The reduction of total ascorbic acid content was detected after 7 days of storage. The ascorbic acid content in 1-MCP-treated sample was slightly higher, although not significantly different, than that of the control after 7 days. These results suggest that the loss of firmness was the key factor affecting quality of fresh-cut muskmelon fruit and the use of 1-MCP maintained the quality and inhibited the loss of firmness by retarding the increase in electrolyte leakage and inhibiting cell wall hydrolases activities.     

Effect of thermosonication on peroxidase,pectin methylesterase activities and on bioactive compounds in custard apple juice

Custard apple juice faces long term storage challenges such as pink discoloration and bitterness due to peroxidase activity. Therefore, inactivation of peroxidase becomes a necessity for longer shelf life of custard apple and its application in food preparations. Heat processing has certain pitfalls such as loss in nutritional contents of custard apple juice, thereby adversely affecting the sensory quality. In the present study, thermosonication of custard apple juice at a constant temperature of 30?±?1?°C (20 kHz, 67.84 W/cm²) from 0 to 40 min has been studied with respect to peroxidase and pectin methylesterase inactivation. Effect of thermosonication has also been studied on bioactive components such as total phenolics and flavonoids along with enzyme inactivation. Complete inactivation of peroxidase and pectin methylesterase with 0% residual activity was achieved within 40 min and 50 s, respectively. Total phenolics were found to increase from 70.9?±?1.6 to 81.7?±?2.5 mg GAE/g. Sonication was found to have no significant effect (P?>?0.05) on pH, °Brix, titratable acidity. Ascorbic acid content remained relatively unaffected during sonication. Hence, thermosonication may be employed as food processing technique where retention of bioactive components have been achieved along with safety and quality attributes.     

Effect of cultivar and fruit storage on basic composition of clear and cloudy pear juices

BackgroundPears due to low acidity are a suitable raw material for the production of multifruit nectars thus it is justified to determine the chemical composition and investigate the effect of cultivar and fruit maturity on juices quality.MethodsJuices from ‘Alexander Lucas’ and ‘Conference’ cultivars were produced after harvest and after 3 months of pears storage.ResultsAverage yield of clear juices was 76.4% for ‘Conference’ cultivar and 74.3% for ‘Alexander Lucas’. In the case of cloudy juices, the yield was lower by about 3%. Processing of ‘Conference’ cv. from stored fruit resulted in yield decrease compared to fruits after harvest; this was not in case of ‘Alexander Lucas’ in 2008, where juices produced from stored fruits were characterized by a higher yield compared to fresh fruit.Total solids content in clear juices was 125–135 g/L and 135–141 g/L for cloudy juices. ABTS^●+ and total phenolics analysis showed that cloudy juices were characterized by a higher antioxidant activity and phenolics content than the clear ones.ConclusionPear storage, on the contrary to apples, does not decrease their suitability for cloudy juice production. Cloudy juices as a rule have higher antioxidant activity than the clear ones.