共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
不同储存时间对普洱茶有机溶剂萃取物清除自由基活性的影响 总被引:2,自引:0,他引:2
本文以半数抑制浓度IC50的大小研究了十种茶叶(不同年份的生普和熟普)有机溶剂萃取物的体外抗氧化活性。结果表明,生茶有机溶剂萃取层清除DPPH自由基、羟基自由基、超氧自由基的IC50最小分别可达0.339 mg/mL、0.074 mg/mL、1.206 mg/mL,熟茶清除DPPH自由基、羟基自由基、超氧自由基的IC50最小分别可达0.554 mg/mL、0.164 mg/mL、1.927 mg/mL,生茶的抗氧化能力明显优于熟茶;各种普洱茶对自由基的清除能力主要取决于各萃取液中的多酚和黄酮含量。生茶乙酸乙酯层的茶多酚萃取量最多,在7.33-9.57%之间,正丁醇层的黄酮类物质的萃取量最多在7.08-10.99%之间,熟茶正丁醇层的茶多酚含量和黄酮类物质的含量在个有机溶剂层中均是最高的,分别在3.62-5.73%之间和4.75-5.83%之间,乙酸乙酯层和正丁醇层的萃取化合物具有较多的羟基,具有较强的清除自由基的能力;茶叶的储存时间对各萃取液的抗氧化能力无显著影响。 相似文献
4.
测定了茶饮料中的茶多酚(Tea Polyphenols,TP)、游离氨基酸总量、表没食子儿茶素没食子酸酯(Epigallocatechi Gallate,EGCG)、咖啡因(Caffeine,CAF)和茶氨酸(L-Theanine,TA)的含量.结果表明:目前市售茶饮料的品质与品牌无关,乌龙茶TP、EGCG和CAF含量最高,乌龙茶和花茶中TP含量完全符合国家标准,绿茶合格率为85.0%,而红茶合格率仅为50.0%.除了两个绿茶饮料样本低于国家标准外,其它样本中的CAF含量全部符合国家标准. 相似文献
5.
不同茶饮产品的抗氧化能力分析及感官评价 总被引:1,自引:0,他引:1
以总还原能力、DPPH·自由基清除率、·OH清除率、O2-·清除率作为指标,评价了不同茶饮产品的抗氧化能力,并按照《GB/T 23776-2009茶叶感官评审方法》的冲泡方法进行10种茶饮产品的感官评价,以讨论其在消费人群中的接受程度。结果表明,总还原能力由强到弱依次为绿毛峰>坨藏茶>竹叶青>昆仑雪菊>康砖>杭白菊>苦荞胚芽茶>干柠檬>苦荞全株茶>鲜柠檬;对DPPH·自由基清除率最高的为干柠檬,达到了93.58%,最低仅为30.13%(鲜柠檬);对·OH清除率最高的为干柠檬95.87%,最低的为苦荞全株茶17.64%;对O2-·清除率最高的为坨藏茶85.86%,最低的为苦荞全株茶9.08%。在感官评价中,对总分以及汤色、香气、滋味三种因子分别分析,竹叶青分值均最高。结果表明,利用不同抗氧化指标表明的抗氧化能力有所区别,总体而言,干柠檬、竹叶青2种茶饮产品表现出较高的抗氧化活性,而苦荞茶较低。此研究结果可为消费者提供对保健茶饮的消费参考。 相似文献
6.
7.
8.
9.
市售茶饮料中主要功效成分分析 总被引:1,自引:0,他引:1
目的研究市售茶饮料中主要品质成分含量情况,并分析其差异原因。方法采用酒石酸铁比色法测定茶多酚(teapolyphenols,TP);采用水合茚三酮比色法测定游离氨基酸总量;采用高效液相色谱法(high performance liquid chromatography, HPLC)法检测6种儿茶素以及咖啡碱(caffeine, CAF)含量。结果目前市售茶饮料以调味茶饮料居多,不同种类、不同品牌茶饮料中的茶多酚、儿茶素、CAF以及游离氨基酸含量具有明显差异。结论绿茶饮料各种主要品质成分含量较高,其次为乌龙茶饮料,红茶饮料较低。CAF含量全部样品符合国家标, TP含量仅有一款红茶饮料低于国家标准,其余均符合国家标。 相似文献
10.
11.
12.
以灵芝红茶为研究对象,应用DPPH·、ABTS~+·和FRAP铁离子还原能力研究灵芝红茶的抗氧化活性,并对灵芝红茶中茶多酚和多糖含量进行测定。结果发现灵芝红茶有清除DPPH自由基、ABTS~+自由基和还原FRAP的作用,表明灵芝红茶具有较强的抗氧化活性。灵芝红茶中的茶多酚和多糖含量测定方法中,没食子酸浓度在4.08μg/mL~16.50μg/mL范围内线性良好,平均回收率分别为99.6%(RSD=2.52%,n=9);葡萄糖浓度在40.33μg/mL~181.50μg/mL范围内线性良好,平均回收率为100.5%(RSD=1.08%,n=9)。所建立的灵芝红茶中茶多酚和多糖的含量测定方法简便快捷,线性、重复性、精密度和回收率良好。此含量测定方法可用于灵芝红茶生产过程中质量控制。 相似文献
13.
14.
E. JOUBERT 《International Journal of Food Science & Technology》1990,25(3):339-343
The effects of extraction temperature (23, 50, 60, 70, 80 and 90°C), mass ratio of extract: dry tea leaf (5:1 and 10:1) and flow rate of water (0.1 and 0.2m3 /h) on extraction of total polyphenols, flavonoids and non-flavonoid phenols from rooibos tea were determined with a single-stage batch-extraction system. Extraction of the different phenolic groups from tea leaves increased significantly with increasing temperature. The increase in total polyphenol extraction was due mainly to increasing extraction of flavonoids. Increasing mass ratio and decreasing flow rate resulted in better extraction of the different phenolic groups. The phenolic content of extract soluble solids also increased significantly with temperature. At extraction temperatures 23 and 50°C, increasing mass ratio resulted in a significant increase in total polyphenol and flavonoid content of the soluble solids. The non-flavonoid phenol content increased significantly with increasing mass ratio at temperatures above 23°C. Flow rate did not significantly affect the total phenolic and flavonoid content of soluble solids. 相似文献
15.
The optimal synchronous conditions to extract tea polysaccharides (TPS) and tea polyphenols (TPP) from Ya'an Tibetan tea were investigated, and the antioxidative capacity of TPS and TPP was measured, and the tea was analyzed to identify the polyphenol compounds it contained. On the basis of single‐factor experiments, a Box–Behnken design and response surface methodology were applied to optimize the hot water extraction conditions. The optimal extraction technology was determined as extraction temperature of 83°C, time of 104 min, and liquid‐to‐material ratio of 41 ml/g, yielding TPP and TPS at 42.70 ± 2.38 mg/g and 53.86 ± 3.79 mg/g, respectively. The TPS and TPP in Ya'an Tibetan tea have high eliminating activities on DPPH and strong reducing power, with TPP showing a higher antioxidant activity than TPS. UHPLC‐QqQ‐MS/MS analysis identified EGCG, GCG, and ECG as major polyphenol components in Ya'an Tibetan tea. These findings might promote the application of Ya'an Tibetan tea in the food industry. 相似文献
16.
17.
建立了食用植物油中5种儿茶素类抗氧化剂的HPLC检测方法。样品经正己烷溶解,再用饱和正己烷的乙腈萃取,振荡,超声25 min,离心后取乙腈层,定容,过0. 45μm滤膜。然后采用Waters XTerra RP-C18色谱柱(4. 6 mm×250 mm,5μm),选择甲醇-水(体积比1∶9)+乙腈作为流动相,流速为1 mL/min,梯度洗脱。综合分析各化合物吸收光谱,选择216、230、280 nm作为检测波长。结果表明:建立的HPLC检测橄榄油中5种儿茶素类化合物的检测方法,化合物分离度好,峰型尖锐,加标回收率在80%~101. 3%之间;方法通用性和精密度实验数据均表明该方法满足检测需要。 相似文献
18.
研究花果山云雾茶中茶多酚和茶多糖的超声辅助同步提取工艺并评价提取物的抗氧化活性。在单因素试验的基础上,响应面分析法优化的提取工艺为:超声功率500 W,提取温度80℃,提取时间37 min,液料比62∶1(mL/g)。在优化的条件下,茶多酚和茶多糖的得率分别为(137.08±3.37)mg/g和(57.71±1.94)mg/g。固态提取物的得率为(2.51±0.27)%,其中茶多酚和茶多糖的含量分别为(548.31±13.48)mg/g和(227.28±6.44)mg/g。通过测定提取物的还原力、抗氧化力和对DPPH自由基、羟自由基及超氧阴离子自由基的清除能力评价其抗氧化活性,结果显示提取物具有较强的还原力和总抗氧化力及清除DPPH自由基、羟自由基和超氧阴离子自由基的活性。 相似文献
19.
《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2013,30(8):1234-1242
Products containing natural additives, including antioxidants, are usually perceived by consumers as safer than those with synthetic ones. Natural antioxidants, besides having a preservative activity, may exert beneficial health effects. Interactions between antioxidants may significantly change their antioxidant activity, thus in designing functional foods or food/cosmetic ingredients knowledge about the type of interactions could be useful. In the present study, the interactions between ascorbic acid (AA; vitamin C) and different black and green tea extracts and the influence on their antioxidant activities were investigated. The antioxidant activities of tea extracts and their mixtures with AA prepared in several different weight ratios were measured using the trolox equivalent antioxidant capacity (TEAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric-reducing antioxidant power (FRAP) methods. The type of interaction was determined by interaction indexes and isobolograms. It was found that the weight ratio of extracts to AA significantly influenced the antioxidant activity of a mixture and the type of interaction between these components. The weight ratio of tea extract to AA can cause the change of interaction, e.g. from antagonism to additivism or from additivism to synergism. The observed differences in the type of interactions were probably also a result of different extracts’ polyphenol composition and content. The type of interaction may also be affected by the medium in which extracts and AA interact, especially its pH and the solvent used. To obtain the best antioxidant effect, all these factors should be taken into account during the design of a tea extract–AA mixture. 相似文献
20.
采用喷雾干燥法,以生物可降解高分子材料羟丙基甲基纤维素邻苯二甲酸酯(HPMCP)为骨架材料,制备茶多酚微球。制备茶多酚微球的最佳条件为:进风温度100℃,进料速度6.25mL/min,芯壁材比为2:1,总固形物含量为3%。茶多酚微球和未包埋的茶多酚有相似的相变峰,说明在微球制备过程中茶多酚未发生变性。茶多酚微球对食用油有较好的抗氧化效果,且随着添加量的增加效果更加明显。 相似文献