离心分配色谱技术及其在天然产物分离中的应用

作为现代逆流色谱的一种，离心分配色谱(CPC)是一种新型的制备色谱技术。简单介绍了离心分配色谱的设备原理和操作方法，综述了离心分配色谱的研究现状和其应用于天然产物分离方面的相关报道，特别对离心分配色谱反应器和离子交换置换离心分配色谱等一些CPC研究领域出现的新技术进行了介绍；对离心分配色谱的研究前景进行了展望。     

色谱分离技术在食品添加剂中的应用

介绍了工业色谱分离技术在国内外食品添加剂工业中,尤其在甜味剂,氨基酸和发酵产品生产中的应用情况,概括了色谱技术在食品添加剂工业中的发展趋势和特点,同时也对在这方面开展的工作进行了初步介绍。     

现代色谱技术在除虫菊素研究中的应用

总结了TLC、GC、HPLC和GC-MS联用等现代色谱技术在除虫菊素各有效组分的分离、制备、结构鉴定和含量测定中的应用。     

现代色谱技术在除虫菊素研究中的应用

总结了TLC、GC、HPLC和GC-MS联用等现代色谱技术在除虫菊素各有效组分的分离、制备、结构鉴定和含量测定中的应用。     

色谱分离技术应用与进展

本文简述了近些年来提出几种比较流行的色谱基础理论，分析了色谱分离技术在实际中的广泛应用及其发展。     

高速逆流色谱在分离纯化中草药黄酮类成分中的应用

高速逆流色谱(HSCCC)技术是国际上较新型的无需固态支撑体的液-液分配色谱技术.介绍了高速逆流色谱的原理、优点、溶剂体系的选择原则、方法,并对其在中草药黄酮类成分分离纯化制备方面的应用进行综述.     

色谱技术进展与应用

本文简要综述了色谱技术及其应用的进展，并对常见的色谱法进行了评述。     

色谱技术在食品安全检测中的应用研究

介绍了色谱及联机技术的基本原理和特点,阐述了色谱及联机技术在食品安全领域的应用及发展.     

色谱分离技术及其应用研究进展

色谱技术作为分离分析的重要方法之一,是分析化学中最富活力的领域之一,能够分离物化性能差别很小的化合物,对蛋白质进行高效率和高灵敏度分离分析研究是生命科学研究的热点领域之一。本文介绍了几种常见色谱技术以及近期发展起来的一些新型色谱技术的研究进展及应用,简要综述了现代色谱技术在蛋白分离和分析中最新应用及进展。     

核酸适配体在蛋白质组学研究中的应用

蛋白质组学是大规模、高通量、系统化从整体水平上研究蛋白质组成、活动规律及蛋白质与蛋白质相互作用的新兴学科。随着高通量核酸适配体筛选技术的发展,具有与抗体结合解离常数相似的上千种酸性、碱性、中性蛋白质的核酸适配体得以筛选。这些核酸适配体广泛应用到固定化酶解反应器、蛋白质免疫印迹、免疫共沉淀、蛋白质与蛋白质相互作用及血清(血浆)、组织、细胞等蛋白质组学研究中。本文简要综述单个核酸适配体及核酸适配体微阵列在蛋白质组学研究中的应用。     

Recent Advances in Proteomic Studies of Adipose Tissues and Adipocytes

Obesity is a chronic disease that is associated with significantly increased levels of risk of a number of metabolic disorders. Despite these enhanced health risks, the worldwide prevalence of obesity has increased dramatically over the past few decades. Obesity is caused by the accumulation of an abnormal amount of body fat in adipose tissue, which is composed mostly of adipocytes. Thus, a deeper understanding of the regulation mechanism of adipose tissue and/or adipocytes can provide a clue for overcoming obesity-related metabolic diseases. In this review, we describe recent advances in the study of adipose tissue and/or adipocytes, focusing on proteomic approaches. In addition, we suggest future research directions for proteomic studies which may lead to novel treatments of obesity and obesity-related diseases.     

Advances of Proteomic Sciences in Dentistry

Applications of proteomics tools revolutionized various biomedical disciplines such as genetics, molecular biology, medicine, and dentistry. The aim of this review is to highlight the major milestones in proteomics in dentistry during the last fifteen years. Human oral cavity contains hard and soft tissues and various biofluids including saliva and crevicular fluid. Proteomics has brought revolution in dentistry by helping in the early diagnosis of various diseases identified by the detection of numerous biomarkers present in the oral fluids. This paper covers the role of proteomics tools for the analysis of oral tissues. In addition, dental materials proteomics and their future directions are discussed.     

Proteomic Studies of Blood and Vascular Wall in Atherosclerosis

The review is devoted to the analysis of literature data related to the role of proteomic studies in the study of atherosclerotic cardiovascular diseases. Diagnosis of patients with atherosclerotic plaques before clinical manifestations is an arduous task. The review presents the results of research on the new proteomic potential biomarkers of coronary heart disease, coronary atherosclerosis, acute coronary syndrome, myocardial infarction, carotid artery atherosclerosis. Also, the analysis of literature data on proteomic studies of the vascular wall was carried out. To assess the involvement of proteins in the pathological process of atherosclerosis, it is important to investigate the specific relationships between proteins in the arteries, expression and concentration of proteins. The development of proteomic technologies has made it possible to analyse the number of proteins associated with the development of the disease. Analysis of the proteomic profile of the vascular wall in atherosclerosis can help to detect possible diagnostically significant protein structures or potential biomarkers of the disease and develop novel approaches to the diagnosis of atherosclerosis and its complications.     

计算机辅助不同溶剂系统两次展开最优化分离

用计算机辅助 ,在两组不同溶剂系统中用两次展开技术与计算机扫描技术相结合来优化展开条件 ,取得了比较满意的结果。     

GC／FTIR技术及其进展

将气相色谱技术的优良分离能力和红外光镰 技术独特的结构鉴别能力相结合的联机检测技术－ＧＣ／ＦＴＩＲ,是一种很有使用价值的分离鉴定手段,本文讨论了气相色谱／傅 红外光谱联用系统的组成及其功能。阐述了发展听几种ＧＣ／ＦＴＩＲ新技术：体隔离技术;冷阳技术;预柱技术以及ＳＦＣ／ＦＴＩＲ技术。同时还论述ＧＣ／ＦＴＩＲ的定量方法。     

精对苯二甲酸装置精制工段浆料分离方法浅析

探讨了精对苯二甲酸(PTA)装置精制工段浆料分离曾使用过的主要方法,分析了几种方法的优缺点,比较了几种方法的综合能耗和成本。结果表明:相比传统离心分离,压力过滤分离在设备一次性投资和维护费用方面有较大优势,一次性投入金额可降低约2 000万元。压力过滤分离工艺中,一步法由于具有流程短、设备数量少的优点,在设备投资和维护成本方面优于二步法,综合能耗和运行费用更是大大降低,吨成品消耗可降低近10 kg标油,每年可节省运行成本600万~800万元,最后还探讨了一步法进一步优化的可能性。     

Urinary Proteomic Signature in Acute Decompensated Heart Failure: Advances into Molecular Pathophysiology

Acute decompensated heart failure (ADHF) is a life-threatening clinical syndrome involving multi-organ function deterioration. ADHF results from multifaceted, dysregulated pathways that remain poorly understood. Better characterization of proteins associated with heart failure decompensation is needed to gain understanding of the disease pathophysiology and support a more accurate disease phenotyping. In this study, we used an untargeted mass spectrometry (MS) proteomic approach to identify the differential urine protein signature in ADHF patients and examine its pathophysiological link to disease evolution. Urine samples were collected at hospital admission and compared with a group of healthy subjects by two-dimensional electrophoresis coupled to MALDI-TOF/TOF mass spectrometry. A differential pattern of 26 proteins (>1.5-fold change, p < 0.005), mostly of hepatic origin, was identified. The top four biological pathways (p < 0.0001; in silico analysis) were associated to the differential ADHF proteome including retinol metabolism and transport, immune response/inflammation, extracellular matrix organization, and platelet degranulation. Transthyretin (TTR) was the protein most widely represented among them. Quantitative analysis by ELISA of TTR and its binding protein, retinol-binding protein 4 (RBP4), validated the proteomic results. ROC analysis evidenced that combining RBP4 and TTR urine levels highly discriminated ADHF patients with renal dysfunction (AUC: 0.826, p < 0.001) and significantly predicted poor disease evolution over 18-month follow-up. In conclusion, the MS proteomic approach enabled identification of a specific urine protein signature in ADHF at hospitalization, highlighting changes in hepatic proteins such as TTR and RBP4.     

分子印迹聚合物在分离技术中的研究进展

由于分子印迹聚合物 (MIP)所具有的独特的预定选择性 ,它在分离技术中的应用研究已受到了普遍关注 ,本文将从MIP的基本概念、制备方法及其在分离技术中的应用等方面对MIP在分离技术中的研究进行简单的评述。引用文献 40篇     

食品中三聚氰胺的检测技术研究现状

三聚氰胺具有66.67%的含氮量,可作为食品中的非法添加物以造成蛋白质含量虚高。由于能够对人体健康造成威胁,且由三聚氰胺引起的相关食品安全事件时有发生,因此开发灵敏、高效和快速的检测方法十分必要。文章旨在综述国内外检测食品中三聚氰胺的相关技术,主要介绍了各种技术的原理和特点。     

电镀废水处理技术研究现状及趋势

分析了电镀废水的来源、特点和危害,介绍了当前常用的电镀废水处理技术,同时对螯合沉淀法和天然矿物污水处理剂在电镀废水治理方面的应用进行简单说明,并结合新的排放标准对电镀废水处理技术的发展趋势进行了展望.