Cluster analysis of antioxidant compounds in dates (Phoenix dactylifera): Effect of long-term cold storage

Both soft (SD, Bam) and dry (DD, Kharak) date varieties were stored at 4 °C for six months followed by an additional one week storage at 18 °C. Antioxidant compounds (total phenolic content (TPC) and total flavonoid content (TFC)) of the dates increased following storage. Cluster analysis applied on TPC and TFC data before and after storage obtained two statistically significant clusters of SD and DD indicating that TPC and TFC had different behaviors according to the types of dates. As antioxidant concentration in dates was dependent on type of dates, selection of variety with high antioxidant compounds may be recommended. Further cold storage of up to six months followed by one week storage at 18 °C may further improve the level of antioxidant compounds.     

Effect of extrusion conditions on resistant starch formation from pastry wheat flour

Pastry wheat flour was extruded under various conditions of feed moisture (20%, 40%, and 60%) and screw speed (150, 200, and 250 rpm), at constant barrel temperature profile (40, 60, 80, 100, and 120 °C, feed port to exit die). The extruded samples were stored at 4 °C for 0, 7, or 14 days, at which times resistant starch (RS) formation was analyzed. Thermal and pasting properties of extruded samples stored for 14 days were analyzed using a differential scanning calorimeter and rapid visco analyzer (RVA), respectively. The RS content increased after extrusion compared to non-extruded pastry wheat flour. High significant positive correlations of feed moisture (P < 0.01) and storage period (P < 0.05) with RS formation were observed. The RS derived from extrusion and storage showed higher thermal stability with decreasing feed moisture and screw speed. Statistically significant differences in pasting properties were observed with feed moisture or screw speed. In particular, the setback value from RVA of the sample was significantly increased with increasing feed moisture. These results indicate that feed moisture and storage time were both important factors for the formation of RS from pastry wheat flour during extrusion.     

The effect of extrusion cooking using different water feed rates on the quality of ready-to-eat snacks made from food by-products

The effect of different levels of feed moisture (12–17%) during extrusion cooking, using a co-rotating twin-screw extruder on selected nutritional and physical properties of extruded products was investigated. Four different formulations were used based on wheat flour and corn starch with the addition of 10% brewer’s spent grain (BSG) and red cabbage (RC) trimming reducing the flour and starch. The samples were: wheat flour + BSG (WBSG), corn starch + BSG (CBSG), wheat flour + red cabbage (WRC) and corn starch + red cabbage (CRC). Process conditions utilised were: constant feed rate of 25 kg/h, screw speed 200 rpm and barrel temperature of 80 and 120 °C. The results indicated that increasing the water feed to 15% increased the level of total dietary fibre (TDF) in all the extrudates while extrusion processing increased the level of TDF in WBSG, CBSG and CRC but decreased in WRC products. Extrusion cooking increased the level of total antioxidant capacity (TAC) and total phenolic compounds (TPC) in WRC and CRC. In addition to water feed level affecting the TDF of the extrudates, also affected were the expansion ratio, bulk density, hardness, WSI, SME and colour. The protein level of the products and hardness of extrudates were related to the different formulations.     

Effects of extrusion conditions on secondary extrusion variables and physical properties of fish, rice-based snacks

A formulation containing rice flour, fish powder, menhaden oil and vitamin E was extruded at a feed rate of 10 kg/h using a co-rotating twin-screw extruder. Primary extrusion (independent) variables were temperature (125-145 °C), screw speed (150-300 rpm) and feed moisture (19-23 g/100 g db). Response surface methodology (RSM) was used to study the effects of extrusion conditions on secondary extrusion variables (product temperature, pressure at the die, motor torque, specific mechanical energy input and mean residence time) and physical properties of the extrudates. Second-order polynomial models were computed and used to generate contour plots. Increasing feed moisture and screw speed decreased pressure at the die. Increased screw speed increased product temperature at the die but increased feed moisture lowered it. Increased barrel temperature, feed moisture and screw speed decreased motor torque. Increased screw speed increased specific mechanical energy, while increased feed moisture reduced it. Longer mean residence times were observed at lower screw speeds. Product density increased as feed moisture increased, but decreased with screw speed. Increased feed moisture decreased radial expansion.     

Effects of cold extrusion process on thiamine and riboflavin contents of fortified corn extrudates

In this study, corn extrudates were produced from fortified corn flour by conventional and cold extrusion techniques at different barrel temperatures of 80, 110, 130 and 80 °C, respectively, and feed moisture contents. Thiamine and riboflavin contents of extrudates were determined by HPLC. Thiamine contents of the samples produced at feed moisture contents 20% and 25% decreased as temperature increased. There was no significant difference between riboflavin contents of conventional extrudates produced at both feed moistures at 80 and 110 °C barrel temperatures. However, riboflavin content of extrudates produced at 20% feed moisture was higher than the one produced at 25% feed moisture at 130 °C. In cold extrusion, there was no significant difference between riboflavin contents of samples. The samples produced by CO₂ injection had the lowest expansion index and uniform air cells. However, the samples produced by conventional extrusion had higher expansion index and size distributions of air cells were not uniform.     

Effects of binary solvent extraction system,extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia)

An investigation into the effects of ethanol concentration (0–100%, v/v), extraction time (20–120 min) and extraction temperature (25–65 °C) on the extraction of phenolic antioxidants from mengkudu (Morinda citrifolia) was performed using a single-factor experiment. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used for determination of phenolic compounds. Antioxidant capacity was evaluated by measuring the scavenging effect on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals. Experimental results showed that extraction conditions had significant effect on extraction of phenolic compounds and antioxidant capacities. The optimised conditions were 40% ethanol for 80 min at 65 °C, with values of 919.95 mg GAE/100 g DW for TPC, 472.73 mg CE/100 g DW for TFC, 791.71 μmol TEAC/100 g DW for ABTS and 1928.5 μmol TEAC/100 g DW for DPPH. TPC was significantly correlated with DPPH under the effects of ethanol concentration (r = 0.932) and extraction time (r = −0.938).     

Structural and textural characterization of corn–lentil extruded snacks

Structural and textural properties of extruded corn and corn–lentil mixtures were investigated as a result of process conditions, including extrusion temperature (170–230 °C), feed rate (2.52–6.84 kg/h) and feed moisture content (13–19% wb). Lentil was used in mixtures with corn flour at a ratio of 10–50% (legume/corn). Apparent density increased with feed rate, moisture content and material ratio and decreased with temperature, while expansion ratio showed an opposite behavior. Modulus of elasticity increased with feed composition and decreased with extrusion conditions. Number of peaks during compression decreased with extrusion temperature and feed composition. Similar behavior was also found for sensory evaluated structural and textural characteristics. The examination of macrostructure confirmed the effects of extrusion conditions and feed composition on structural and textural properties. The properties correlation revealed critical relationships among instrumental and sensorial characteristics. Hence, simple power model equations were developed, which enable their prediction and consequently the design of extruded snacks with acceptable quality characteristics.     

The effect of feed moisture and temperature on tannin content,antioxidant and antimicrobial activities of extruded chestnuts

This study focuses on the effect of extrusion processing on tannin reduction, phenolic content, flavonoid content, antioxidant and anitimicrobial activity. Extrusion temperature (120 and 140 °C) and feed moisture (25% and 28%) were used on the tannin content, antioxidant and antimicrobial activities. Extrusion cooking reduced tannin content up to 78%, and improved antioxidant activity from 12.89% to 21.17% in a concentration dependant manner without affecting its antimicrobial activity that varied from 250 to 500 mg. The time–kill assay confirmed the ability of extruded chestnut to reduce Pseudomonas aeruginosa count below detectable limit that reduced the original inoculum by 3log10 CFU/mL. Overall, the results showed that extrusion cooking might serve as a tool for tannin reduction and could improve the antioxidant and antimicrobial properties of chestnut, which might be helpful for chestnut related products in the food industry.     

Antioxidant and polyphenol oxidase activity of germinated barley and its milling fractions

Eight different hulled barley cultivars were evaluated before and after germination for total phenolic content (TPC), antioxidant activity (AOA) and polyphenol oxidase (PPO) activity. Germination for 12 h significantly decreased the TPC in whole flour (WF), bran (B) and refined flour (RF), however, further increase in germination to 24 h significantly increased the TPC in the fractions. Germination for 12 h duration significantly increased the AOA for WF, B and RF and further increase in germination to 24 h significantly increased the AOA in all the barley cultivars tested. A strong positive correlation was observed between AOA and TPC. Upon germination for 12 h the polyphenol oxidase activity of WF, B and RF significantly decreased by 13.31–59.22, 1.93–59.46 and 7.08–45.87%, respectively, however, the PPO activity increased upon germination for 24 h. The highest level of TPC and AOA was observed in the bran fraction of all the barley cultivars.     

Effect of heating conditions of grape seeds on the antioxidant activity of grape seed extracts

This study was carried out to evaluate the effect of heating and physical conditions of grape seeds on the antioxidant activity of their extracts. Two forms of grape seeds, whole and powdered forms, were heated at four different temperatures −50, 100, 150 and 200 °C. After heating, grape seeds were extracted with 70% ethanol (0.1 g grape seed/10 mL of 70% ethanol), and total phenol contents (TPC), radical scavenging activity (RSA) and reducing power of the extracts were determined. Thermal treatment of grape seed increased the antioxidant activity of extracts. The maximum TPC and RSA of whole grape seed extract (WGSE) were achieved when the seeds were heat-treated at 150 °C for 40 min, while that of powdered grape seed extract (PGSE) were at 100 °C for 10 min, and were greater than that of the non-treated control. Also, the reducing powers of WGSE and PGSE slightly increased at the conditions. According to the GC-MS analysis, several low-molecular-weight phenolic compounds such as azelaic acid, 3,4-dihydroxy benzoic acid, and o-cinnamic acid were newly formed in the WGSE heated at 150 °C for 40 min. There were slight differences in the kinds of phenolic compounds between non-heated and heated GSE. In HPLC analysis, the contents of gallocatechin gallate and caffeine in GSE significantly increased by heat treatment. These results indicated that antioxidant activity of GSE was affected by heating conditions (temperature and time) and physical conditions of grape seeds at the time of heat treatments.     

Effects of moisture,temperature and level of pea grits on extrusion behaviour and product characteristics of rice

A study was conducted to investigate the effect of feed moisture (18–24%), extrusion temperature (130–170 °C) and level of pea grits (0–30%) on the extrusion behaviour and extrudate properties of rice grits. The extruder die pressure, specific energy consumption, expansion ratio, density, water absorption index, and water solubility index were studied. Second-order polynomials were compared for extruder parameters and product characteristics as a function of feed moisture, extrusion temperature and pea grit level.     

Antioxidant potential of wheat flour chapattis as affected by incorporating barley flour

Whole wheat flour was replaced with whole barley flour at levels of 28, 56 and 84 g/100 g to prepare chapattis and their antioxidant properties were evaluated before and after baking. The total phenolic content (TPC) in wheat flour was 2062 μg ferulic acid equivalents/g, total flavonoid content (TFC) was 966 μg catechin equivalents/g and antioxidant activity was 12.3% and they increased by 57.1, 101 and 22.6%, respectively upon incorporating barley flour at the highest level. Baking significantly decreased the TPC by up to 17.0% and TFC by up to 30.7% however the AOA increased by 13%. Barley flour incorporation significantly increased the reducing power which upon baking showed an insignificant decrease. Polyphenol oxidase (PPO) activity significantly increased by up to 109% upon incorporating barley flour however baking lowered PPO activity by 67.9%. Chapattis containing barley flour can be used to deliver the bioactive components of barley.     

Extrusion of a hard-to-cook bean (Phaseolus vulgaris L.) and quality protein maize (Zea mays L.) flour blend

Heated extrusion was tested as an alternative process for incorporating “hard-to-cook” beans into food products. A 3² factorial design was used to evaluate extrusion conditions for a 40/60 (w/w) blend of “hard-to-cook” beans and quality protein maize. Tested extrusion variables were temperature (155, 170 and 185 °C) and moisture content (15.5, 17.5 and 19.5 g/100 g). Screw speed was fixed at 130 rpm. The extrudates obtained at 155 and 170 °C with 15.5% moisture had the best physical characteristics and were chosen for comparative analysis of nutritional changes between the unprocessed “hard-to-cook” bean/quality protein maize flour blend and the resulting extrudates. In vitro protein digestibility was higher in the extrudates (80%) than in the flour blend (76%). In vitro starch digestibility was higher at 155 °C (89%) and 170 °C (92%) than in the flour blend (12%). Processing conditions decreased dietary fibre content by 38% at 155 °C and 44% at 170 °C.     

A wild strain of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) from farm-stored maize in South Carolina: Development under different temperature, moisture, and dietary conditions

The purpose of this study was to determine the duration of immature development and survivorship of Plodia interpunctella (Hübner) on maize over a range of temperatures and grain moisture contents encountered in maize stored on farms in the southeastern states (USA). Laboratory cultures were established with moths collected from farm-stored maize in South Carolina and maintained on cracked maize at 30 °C and 60% r.h. The incubation period and percentage hatch of eggs was determined at 18 combinations of temperature and r.h. Hatch was <1% at 15 and 40 °C. In the range 20-35 °C, percentage hatch declined as temperature increased, and the mean incubation period ranged from 3.1 to 8.5 d. Neither percentage hatch nor incubation period were affected by r.h. between 43% and 76%. The relationship between mean developmental period (oviposition to adult eclosion) and temperature was well described by a quadratic polynomial that predicted a decline from 67.6 to 30.1 d as temperature increased from 20 to 31.1 °C, followed by an increase to 38.5 d as temperature increased further to 35 °C. The results suggest a lower temperature threshold for development near 15 °C and an upper limit slightly greater than 35 °C. Moisture content had a significant effect on developmental period at all the temperatures studied, but the pattern of variation with moisture depended upon the temperature.     

Impact of limited drying on Momordica cochinchinensis Spreng. aril carotenoids content and antioxidant activity

Food product based on gac fruit (Momordica cochinchinensis Spreng.) arils have a high potential due to the high carotenoids content of this fruit. Drying is a key preparation step for carotenoids extraction from gac fruit in a economically viable process. The impact of different drying technics, temperature, final product moisture content on the carotenoid content, hydrophilic and lipophilic antioxidant activity (evaluated with three methods) and color of the gac arils is discussed based on laboratory scale experimental tests. The results highlight an optimal temperature between 50 °C and 60 °C to conserve the color, the carotenoid content and the antioxidant activity. Also, these properties are better preserved by limiting the drying to dry based moisture content between 15% and 18% while the advantages of drying for further processing and for refrigerated conservation for a few months are achieved.     

The role of polyphenol oxidase and peroxidase in the browning of water caltrop pericarp during heat treatment

The mechanism of browning involving enzymatic browning was investigated in the pericarp of water caltrop, an Asian vegetable popular for its taste and medicinal properties. Polyphenol oxidase (PPO) and peroxidase (POD) activities were determined in pericarp at various times and temperatures. Water caltrop consisted of 44.22% moisture content, 37.23% crude fibre, and 2.63% crude protein. PPO and POD activities dropped from 62 and 38 units/g sample, respectively, as water temperature was increased from 30 to 80 °C. Optimum pH and temperature for PPO activity was at pH 5.0, 25–45 °C, and POD activity peaked at 60 °C. High PPO and POD activities at 40–50 °C resulted in degradation of phenolic compounds, which led to increased aggregation of browning pigments and discolouration (lower L-values) of the pericarp. Enzymatic browning was determined as the major factor in the browning discolouration of heat-treated water caltrop pericarp.     

Application of osmotic membrane distillation process in red grape juice concentration

The osmotic membrane distillation process was applied in dehydration of red grape juice (Most de Raïm Concentrat Negre) of different concentrations (5–20 °Brix). Experiments were performed using polytetrafluoroethylene (PTFE) hydrophobic membranes of different nominal pore diameter. Red grape juice solution and concentrated calcium chloride solution (50 wt.%) were used as feed and stripping solutions, respectively. The permeate flux, the initial and the final juice concentration, the total phenolic content (TPC) and the antioxidant activity (TEAC) were measured for each single experiment.